The gene of Helicobacter pylori can encode three to four type IV secretory systems,of which a new gene region has been found in the H.pylori plasticity region.The coding products of this region can form a new T4SS nam...The gene of Helicobacter pylori can encode three to four type IV secretory systems,of which a new gene region has been found in the H.pylori plasticity region.The coding products of this region can form a new T4SS named tfs3,but its function is unclear.This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H.pylori clinical strain SBK on GES-1 cells.We observed changes in cell morphology after VirD4 treatment.Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB.VirD4 can also inhibited proliferation,and induced migration of cells.Moreover,VirD4 caused apoptosis in GES-1 cells in caspase and ERK1/2/Ras dependent signaling events.Our study laid a foundation for further research on the biological function of VirD4 and the detection and treatment of H.pylori-related diseases.展开更多
Tea polyphenols have been shown to have anticancer activity in many studies.In the present study,we investigated effects of theaflavin-3-3'-digallate(TF3),one of the major theaflavin monomers in black tea,in combi...Tea polyphenols have been shown to have anticancer activity in many studies.In the present study,we investigated effects of theaflavin-3-3'-digallate(TF3),one of the major theaflavin monomers in black tea,in combination with ascorbic acid(AA),a reducing agent,and(-)-epigallocatechin-3-gallate(EGCG),the main polyphenol presented in green tea,in combination with AA on cellular viability and cell cycles of the human lung adenocarcinoma SPC-A-1 cells.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay showed that the 50% inhibition concentrations(IC50) of TF3,EGCG,and AA on SPC-A-1 cells were 4.78,4.90,and 30.62 μmol/L,respectively.The inhibitory rates of TF3 combined with AA(TF3+AA) and EGCG combined with AA(EGCG+AA) at a molar ratio of 1:6 on SPC-A-1 cells were 54.4% and 45.5%,respectively.Flow cytometry analysis showed that TF3+AA and EGCG+AA obviously increased the cell population in the G0/G1 phase of the SPC-A-1 cell cycle from 53.9% to 62.8% and 60.0%,respectively.TF3-treated cells exhibited 65.3% of the G0/G1 phase at the concentration of its IC50.Therefore,TF3+AA and EGCG+AA had synergistic inhibition effects on the proliferation of SPC-A-1 cells,and significantly held SPC-A-1 cells in G0/G1 phase.The results suggest that the combination of TF3 with AA or EGCG with AA enhances their anticancer activity.展开更多
基金This work was supported by the Natural Science Foundation of Jiangsu Province(H2018072)Taizhou Social Development Project(TS201623).
文摘The gene of Helicobacter pylori can encode three to four type IV secretory systems,of which a new gene region has been found in the H.pylori plasticity region.The coding products of this region can form a new T4SS named tfs3,but its function is unclear.This study investigated the effect of VirD4 recombinant protein in the tfs3 secretory system of the H.pylori clinical strain SBK on GES-1 cells.We observed changes in cell morphology after VirD4 treatment.Further analysis indicated that VirD4 increased inflammation by increasing the activation of NF-κB.VirD4 can also inhibited proliferation,and induced migration of cells.Moreover,VirD4 caused apoptosis in GES-1 cells in caspase and ERK1/2/Ras dependent signaling events.Our study laid a foundation for further research on the biological function of VirD4 and the detection and treatment of H.pylori-related diseases.
基金supported by the Key Program of Science and Technology of Zhejiang Province(No.2007C12068)of Chinathe National Natural Science Foundation of China(No.30901002)
文摘Tea polyphenols have been shown to have anticancer activity in many studies.In the present study,we investigated effects of theaflavin-3-3'-digallate(TF3),one of the major theaflavin monomers in black tea,in combination with ascorbic acid(AA),a reducing agent,and(-)-epigallocatechin-3-gallate(EGCG),the main polyphenol presented in green tea,in combination with AA on cellular viability and cell cycles of the human lung adenocarcinoma SPC-A-1 cells.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay showed that the 50% inhibition concentrations(IC50) of TF3,EGCG,and AA on SPC-A-1 cells were 4.78,4.90,and 30.62 μmol/L,respectively.The inhibitory rates of TF3 combined with AA(TF3+AA) and EGCG combined with AA(EGCG+AA) at a molar ratio of 1:6 on SPC-A-1 cells were 54.4% and 45.5%,respectively.Flow cytometry analysis showed that TF3+AA and EGCG+AA obviously increased the cell population in the G0/G1 phase of the SPC-A-1 cell cycle from 53.9% to 62.8% and 60.0%,respectively.TF3-treated cells exhibited 65.3% of the G0/G1 phase at the concentration of its IC50.Therefore,TF3+AA and EGCG+AA had synergistic inhibition effects on the proliferation of SPC-A-1 cells,and significantly held SPC-A-1 cells in G0/G1 phase.The results suggest that the combination of TF3 with AA or EGCG with AA enhances their anticancer activity.
