Three hundred thirty-six Ross 308 male broiler chicks were used in a 21-d study to explore performance and gut function when treated with a proton pump inhibitor(PPI;0 or 89 mg/kg)in a 2×2 factorial arrangement w...Three hundred thirty-six Ross 308 male broiler chicks were used in a 21-d study to explore performance and gut function when treated with a proton pump inhibitor(PPI;0 or 89 mg/kg)in a 2×2 factorial arrangement with a xylanase(Xyl;0 or 0.1 g/kg)to determine if the beneficial activity of arabinoxylan(AX)depolymerisation,through arabinoxylo-oligosaccharides(AXOS)production,starts in the upper gastrointestinal tract.Treatment with the PPI started from d 14,and by d 21 animal performance had deteriorated(P<0.001).An interaction was observed between PPI and Xyl for feed conversion ratio(FCR)(P<0.05),whereby the combination reduced the negative effect of PPI on FCR.Application of PPI raised digesta pH in the gizzard and caecum(P<0.05),increased protein concentrations in the lower gut(P<0.05)and reduced intake of digestible nutrients(P<0.05).Caecal concentrations of indole,p-cresol,ammonia and the ratio of total volatile fatty acid(VFA)to butyric acid were increased with PPI(P<0.05),indicating enhanced protein fermentation.Xylanase activity in the digesta were greatest in the caeca,especially when Xyl was supplemented(P<0.001).The concentration of total soluble AX was greater in the gizzard and ileal digesta with Xyl supplementation(P<0.05),supporting the depolymerisation action of xylanase even under acidic conditions.These data suggest xylanase may function in the gizzard even though pH is not optimal for activity and emphasises the importance of chlorohydric acid secretions in ensuring overall optimum gut function.AX depolymerisation benefits animal performance although it is still unknown how the AXOS produced with xylanase supplementation in the upper gastrointestinal tract influence the microbial populations and overall gut functionality.展开更多
研究利用毕赤酵母GS115表达的小麦木聚糖酶抑制蛋白,探索了该XIP抑制蛋白对GH10和GH11家族木聚糖酶的抑制作用。结果显示,该抑制蛋白分子量约为37 k Da,高于氨基酸序列计算所得分子量30.2 k Da,可能是由于该蛋白发生了糖基化作用。该蛋...研究利用毕赤酵母GS115表达的小麦木聚糖酶抑制蛋白,探索了该XIP抑制蛋白对GH10和GH11家族木聚糖酶的抑制作用。结果显示,该抑制蛋白分子量约为37 k Da,高于氨基酸序列计算所得分子量30.2 k Da,可能是由于该蛋白发生了糖基化作用。该蛋白分别作用于黑曲霉GH10木聚糖酶和哈茨木霉GH11木聚糖酶,得到最适抑制比例分别为1.2??1和8??1,最适抑制时间分别是50 min和30 min,最适抑制p H值均为6.0,最适作用温度均是40℃。展开更多
基金This research was financially supported by AB Vista.The authors wish to thank the Physiology Professor Dra.Patri Vergara,from the Veterinary School of the Universitat Autonoma de Barcelona(Spain)for the technical advice in the selection of the best model to apply in chickens at the beginning of the project.
文摘Three hundred thirty-six Ross 308 male broiler chicks were used in a 21-d study to explore performance and gut function when treated with a proton pump inhibitor(PPI;0 or 89 mg/kg)in a 2×2 factorial arrangement with a xylanase(Xyl;0 or 0.1 g/kg)to determine if the beneficial activity of arabinoxylan(AX)depolymerisation,through arabinoxylo-oligosaccharides(AXOS)production,starts in the upper gastrointestinal tract.Treatment with the PPI started from d 14,and by d 21 animal performance had deteriorated(P<0.001).An interaction was observed between PPI and Xyl for feed conversion ratio(FCR)(P<0.05),whereby the combination reduced the negative effect of PPI on FCR.Application of PPI raised digesta pH in the gizzard and caecum(P<0.05),increased protein concentrations in the lower gut(P<0.05)and reduced intake of digestible nutrients(P<0.05).Caecal concentrations of indole,p-cresol,ammonia and the ratio of total volatile fatty acid(VFA)to butyric acid were increased with PPI(P<0.05),indicating enhanced protein fermentation.Xylanase activity in the digesta were greatest in the caeca,especially when Xyl was supplemented(P<0.001).The concentration of total soluble AX was greater in the gizzard and ileal digesta with Xyl supplementation(P<0.05),supporting the depolymerisation action of xylanase even under acidic conditions.These data suggest xylanase may function in the gizzard even though pH is not optimal for activity and emphasises the importance of chlorohydric acid secretions in ensuring overall optimum gut function.AX depolymerisation benefits animal performance although it is still unknown how the AXOS produced with xylanase supplementation in the upper gastrointestinal tract influence the microbial populations and overall gut functionality.
文摘研究利用毕赤酵母GS115表达的小麦木聚糖酶抑制蛋白,探索了该XIP抑制蛋白对GH10和GH11家族木聚糖酶的抑制作用。结果显示,该抑制蛋白分子量约为37 k Da,高于氨基酸序列计算所得分子量30.2 k Da,可能是由于该蛋白发生了糖基化作用。该蛋白分别作用于黑曲霉GH10木聚糖酶和哈茨木霉GH11木聚糖酶,得到最适抑制比例分别为1.2??1和8??1,最适抑制时间分别是50 min和30 min,最适抑制p H值均为6.0,最适作用温度均是40℃。
基金Supported by the National Natural Science Foundation of China(Nos.30971702 and 31271632)Science and Technology Department of Zhejiang Province,China(Nos.2013C32018 and 2016C32086)
基金National Natural Science Foundation of China (30971702, 31271632, and 31672462)Research Grants from the Science and Technology Department of Zhejiang Province,China (LGN19C130004)。
