The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant insects.In the construction of CRISPR/Cas9-mediated dsLmRNase2^(–/–)mutant locusts,we...The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant insects.In the construction of CRISPR/Cas9-mediated dsLmRNase2^(–/–)mutant locusts,we found that the tanned eggs which experienced a 20-min contact with the oocyst exhibited a higher success rate compared to fresh newly-laid eggs that were less tanned.However,the heritable efficiency of the dsLmRNase2 deletion to the next generation G_(1 )progeny was similar between adults derived from the tanned or less tanned engineered eggs.Further,the similar effective mutant ratios in the normally developed eggs and G_(0) adults of tanned and less tanned eggs also indicated that tanning did not reduce the absolute mutation efficiency induced by CRISPR/Cas9.Moreover,we found that the syncytial division period,which was longer than the time for tanning,conferred a window period for microinjection treatment with efficient mutation in both tanned and less tanned eggs.We further found that tanned eggs exhibited a higher hatching rate due to a reduced infection rate following microinjection.Both the anti-pressure and ultrastructure analyses indicated that the tanned eggs contained compressed eggshells to withstand increased external pressure.In summary,tanned eggs possess stronger defense responses and higher efficiency of genome editing,providing an improved model for developing Cas9-mediated gene editing procedures in locusts.展开更多
With the ever-expanding applications of vehicles and the development of wireless communication technology,the burgeoning unmanned aerial vehicle(UAV)assisted vehicular internet of things(UVIoTs)has emerged,where the g...With the ever-expanding applications of vehicles and the development of wireless communication technology,the burgeoning unmanned aerial vehicle(UAV)assisted vehicular internet of things(UVIoTs)has emerged,where the ground vehicles can experience more efficient wireless services by employing UAVs as a temporary mobile base station.However,due to the diversity of UAVs,there exist UAVs such as jammers to degenerate the performance of wireless communication between the normal UAVs and vehicles.To solve above the problem,in this paper,we propose a game based secure data transmission scheme in UVIoTs.Specifically,we exploit the offensive and defensive game to model the interactions between the normal UAVs and jammers.Here,the strategy of the normal UAV is to determine whether to transmit data,while that of the jammer is whether to interfere.We then formulate two optimization problems,i.e.,maximizing the both utilities of UAVs and jammers.Afterwards,we exploit the backward induction method to analyze the proposed countermeasures and finally solve the optimal solution.Lastly,the simulation results show that the proposed scheme can improve the wireless communication performance under the attacks of jammers compared with conventional schemes.展开更多
<strong>Introduction</strong><strong>:</strong> The key to success is finding the perfect mixture of tactical patterns and sudden breaks of them, which depends on the behavior of the opponent t...<strong>Introduction</strong><strong>:</strong> The key to success is finding the perfect mixture of tactical patterns and sudden breaks of them, which depends on the behavior of the opponent team and is not easy to estimate by just watching matches. According to the specific tactical team behavior of “attack vs. defense” professional football matches are investigated based on a simulation approach, professional football matches are investigated according to the specific tactical team behavior of “attack vs. defense.” <strong>Methods:</strong> The formation patterns of all the sample games are categorized by SOCCER<span style="white-space:nowrap;">©</span> for defense and attack. Monte Carlo-Simulation can evaluate the mathematical, optimal strategy. The interaction simulation between attack and defense shows optimal flexibility rates for both tactical groups. <strong>Approach: </strong>A simulation approach based on 40 position data sets of the 2014/15 German Bundesliga has been conducted to analyze and optimize such strategic team behavior in professional soccer. <strong>Results:</strong> The results revealed that both attack and defense have optimal planning rates to be more successful. The more complex the success indicator, the more successful attacking player groups get. The results also show that defensive player groups always succeed in attacking groups below a specific planning rate value.<strong> Conclusion:</strong> Groups are always succeeding. The simulation-based position data analysis shows successful strategic behavior patterns for attack and defense. Attacking player groups need very high flexibility to be successful (stay in ball possession). In contrast, defensive player groups only need to be below a defined flexibility rate to be guaranteed more success.展开更多
基金This work was supported by the National Natural Science Foundation of China(32070502,31730074,32072419 and 31501905).
文摘The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant insects.In the construction of CRISPR/Cas9-mediated dsLmRNase2^(–/–)mutant locusts,we found that the tanned eggs which experienced a 20-min contact with the oocyst exhibited a higher success rate compared to fresh newly-laid eggs that were less tanned.However,the heritable efficiency of the dsLmRNase2 deletion to the next generation G_(1 )progeny was similar between adults derived from the tanned or less tanned engineered eggs.Further,the similar effective mutant ratios in the normally developed eggs and G_(0) adults of tanned and less tanned eggs also indicated that tanning did not reduce the absolute mutation efficiency induced by CRISPR/Cas9.Moreover,we found that the syncytial division period,which was longer than the time for tanning,conferred a window period for microinjection treatment with efficient mutation in both tanned and less tanned eggs.We further found that tanned eggs exhibited a higher hatching rate due to a reduced infection rate following microinjection.Both the anti-pressure and ultrastructure analyses indicated that the tanned eggs contained compressed eggshells to withstand increased external pressure.In summary,tanned eggs possess stronger defense responses and higher efficiency of genome editing,providing an improved model for developing Cas9-mediated gene editing procedures in locusts.
基金This work is supported in part by NSFC(nos.U1808207,U20A20175)the Project of Shanghai Municipal Science and Technology Commission(18510761000).
文摘With the ever-expanding applications of vehicles and the development of wireless communication technology,the burgeoning unmanned aerial vehicle(UAV)assisted vehicular internet of things(UVIoTs)has emerged,where the ground vehicles can experience more efficient wireless services by employing UAVs as a temporary mobile base station.However,due to the diversity of UAVs,there exist UAVs such as jammers to degenerate the performance of wireless communication between the normal UAVs and vehicles.To solve above the problem,in this paper,we propose a game based secure data transmission scheme in UVIoTs.Specifically,we exploit the offensive and defensive game to model the interactions between the normal UAVs and jammers.Here,the strategy of the normal UAV is to determine whether to transmit data,while that of the jammer is whether to interfere.We then formulate two optimization problems,i.e.,maximizing the both utilities of UAVs and jammers.Afterwards,we exploit the backward induction method to analyze the proposed countermeasures and finally solve the optimal solution.Lastly,the simulation results show that the proposed scheme can improve the wireless communication performance under the attacks of jammers compared with conventional schemes.
文摘<strong>Introduction</strong><strong>:</strong> The key to success is finding the perfect mixture of tactical patterns and sudden breaks of them, which depends on the behavior of the opponent team and is not easy to estimate by just watching matches. According to the specific tactical team behavior of “attack vs. defense” professional football matches are investigated based on a simulation approach, professional football matches are investigated according to the specific tactical team behavior of “attack vs. defense.” <strong>Methods:</strong> The formation patterns of all the sample games are categorized by SOCCER<span style="white-space:nowrap;">©</span> for defense and attack. Monte Carlo-Simulation can evaluate the mathematical, optimal strategy. The interaction simulation between attack and defense shows optimal flexibility rates for both tactical groups. <strong>Approach: </strong>A simulation approach based on 40 position data sets of the 2014/15 German Bundesliga has been conducted to analyze and optimize such strategic team behavior in professional soccer. <strong>Results:</strong> The results revealed that both attack and defense have optimal planning rates to be more successful. The more complex the success indicator, the more successful attacking player groups get. The results also show that defensive player groups always succeed in attacking groups below a specific planning rate value.<strong> Conclusion:</strong> Groups are always succeeding. The simulation-based position data analysis shows successful strategic behavior patterns for attack and defense. Attacking player groups need very high flexibility to be successful (stay in ball possession). In contrast, defensive player groups only need to be below a defined flexibility rate to be guaranteed more success.
