The concentrations of bovine serum albumin,gliadin,bovine hemoglobim,mucin,lysozyme and proteins mixture in solutions were determined by spectrophotometric assay at 230 nm(A230 assay).For all the sample solutions of p...The concentrations of bovine serum albumin,gliadin,bovine hemoglobim,mucin,lysozyme and proteins mixture in solutions were determined by spectrophotometric assay at 230 nm(A230 assay).For all the sample solutions of pure proteins,the linear range of A230assay was 25～250μg·mL-1,and salt had little influence on this method.For the solutions containing two kinds of proteins,the mass ratio of the proteins affected the result of determination.For the solutions of proteins mixture,the average recovery rate(n=6)of A230assay was 101.6%(99.04%～109.1%)with RSD=3.82%.As a result,a new effective method to determine protein concentration was suggested.展开更多
目的:测定硫酸阿托品胶浆剂中硫酸阿托品的含量。方法:采用二阶导数分光光度法,测定波长为258 nm。结果:硫酸阿托品在0.1～1.4 mg·mL^(-1)浓度范围内与峰-零振幅值 D 呈良好的线性关系(r=0.9999),平均回收率为99.6%(n=6)。结论:该...目的:测定硫酸阿托品胶浆剂中硫酸阿托品的含量。方法:采用二阶导数分光光度法,测定波长为258 nm。结果:硫酸阿托品在0.1～1.4 mg·mL^(-1)浓度范围内与峰-零振幅值 D 呈良好的线性关系(r=0.9999),平均回收率为99.6%(n=6)。结论:该方法简便、快速、结果准确,适于该制剂的质量控制。展开更多
文摘The concentrations of bovine serum albumin,gliadin,bovine hemoglobim,mucin,lysozyme and proteins mixture in solutions were determined by spectrophotometric assay at 230 nm(A230 assay).For all the sample solutions of pure proteins,the linear range of A230assay was 25～250μg·mL-1,and salt had little influence on this method.For the solutions containing two kinds of proteins,the mass ratio of the proteins affected the result of determination.For the solutions of proteins mixture,the average recovery rate(n=6)of A230assay was 101.6%(99.04%～109.1%)with RSD=3.82%.As a result,a new effective method to determine protein concentration was suggested.
文摘目的:测定硫酸阿托品胶浆剂中硫酸阿托品的含量。方法:采用二阶导数分光光度法,测定波长为258 nm。结果:硫酸阿托品在0.1～1.4 mg·mL^(-1)浓度范围内与峰-零振幅值 D 呈良好的线性关系(r=0.9999),平均回收率为99.6%(n=6)。结论:该方法简便、快速、结果准确,适于该制剂的质量控制。
