Aim To investigate the role of the Chinese herbal medicine Xianhuayin on the reversal of 7,12-dimethylbenz[a]anthracene （DMBA）-induced premalignant mucosal lesions in the oral buccal pouch of golden hamsters. Method...Aim To investigate the role of the Chinese herbal medicine Xianhuayin on the reversal of 7,12-dimethylbenz[a]anthracene （DMBA）-induced premalignant mucosal lesions in the oral buccal pouch of golden hamsters. Methodology The animals were randomly divided into a non-diseased control group （n=5） and an experimental group including 50 animals in which the buccal mucosa had been painted with DMBA （0.5% in acetone） to generate an oral mucosa premalignant lesion. Animals in the experi- mental group were further divided into Xianhuayin-treated group （n=30）, untreated prem＇alignant lesion group （n=10） and normal saline （NS）-treated group （n=10）. The cheek （buccal） pouch mucosa of the golden hamsters in each group was observed with light and electron microscopy eight weeks after intragastric administration with NS or Xianhuayin. Results In the non-diseased control group, the buccal mucosa was keratinized and stratified squamous epithelium under a light microscope. In the untreated premalignant lesion group, variable degrees of epithelial dysplasia was observed. The irregular epithelial mucosa gradually became distinct in the Xianhuayin-treated group. Scanning electronic microscopic （SEM） analysis showed that surface of the cells exhibited honeycomb structures in the hamster of untreated- group. The cells were morphologically irregular, overlapped and loosened in the untreated premalignant lesion group. Most of the cell surface exhibited honeycomb structure in the Xianhuayin-treated group. Transmission electronic micro- scopic （TEM） analysis showed that buccal mucosal epithelial cells were morphologically regular in the non-diseased control group. Desmosomes and tonofibrils were reduced and the nucleus was morphologically irregular in the untreated premalignant lesion group. In the Xianhuayin-treated group, the widening intercellular gap was gradually reduced, desmosomes and the cells becoming morphologically regular. No significant difference was observed between the hamsters in NS-treated group and those in the untreated premalignant lesion group. Significant therapeutic efficacy was observed in the group receiving Xianhuayin. Conclusion Xianhuayin is effective in the reversal of DMBA-induced premalignant lesions in the buccal pouch of golden hamsters.展开更多
AIM:To explore the model of methotrexate(MTX)-induced mic e oral mucosa lesions METHOD:A total of 282 mice were randomly d ivided into seven groups as follows:control,MTX10,20,30,40,50,75 mg/ml The physiological salin...AIM:To explore the model of methotrexate(MTX)-induced mic e oral mucosa lesions METHOD:A total of 282 mice were randomly d ivided into seven groups as follows:control,MTX10,20,30,40,50,75 mg/ml The physiological saline solution (05 ml) was ip injecte d into mice in control group The dose of MTX (05 ml) was ip injected int o mic e in other groups only once During 10 consecutive days after administration of MTX,3 mice were sacrificed in each day in all the groups and the patholog ical chang es of oral mucosa were examed under the optical microscope RE SULT: The incidence(45%～63%) and the integral rate(194%～56%) of mice ora l mu cosa lesions in group of intermediate-dose MTX (IDMTX) (20～40 mg/kg) were sign ifi cantly higher than those in other groups The death rate (not more than 5%) in group of IDMTX was lower than that in other groups CONCLUSION:As a result of induction of higher lesion rate and lower death rate of mice oral mucosa by IDMTX (2 0～40 mg/kg),the model of IDMTX-induced oral mucosa lesion could be applie d into clinical or basic展开更多
基金supported by grants from the highly distinguished Oncology Subject Foundation of Colleges in Hebei Province
文摘Aim To investigate the role of the Chinese herbal medicine Xianhuayin on the reversal of 7,12-dimethylbenz[a]anthracene （DMBA）-induced premalignant mucosal lesions in the oral buccal pouch of golden hamsters. Methodology The animals were randomly divided into a non-diseased control group （n=5） and an experimental group including 50 animals in which the buccal mucosa had been painted with DMBA （0.5% in acetone） to generate an oral mucosa premalignant lesion. Animals in the experi- mental group were further divided into Xianhuayin-treated group （n=30）, untreated prem＇alignant lesion group （n=10） and normal saline （NS）-treated group （n=10）. The cheek （buccal） pouch mucosa of the golden hamsters in each group was observed with light and electron microscopy eight weeks after intragastric administration with NS or Xianhuayin. Results In the non-diseased control group, the buccal mucosa was keratinized and stratified squamous epithelium under a light microscope. In the untreated premalignant lesion group, variable degrees of epithelial dysplasia was observed. The irregular epithelial mucosa gradually became distinct in the Xianhuayin-treated group. Scanning electronic microscopic （SEM） analysis showed that surface of the cells exhibited honeycomb structures in the hamster of untreated- group. The cells were morphologically irregular, overlapped and loosened in the untreated premalignant lesion group. Most of the cell surface exhibited honeycomb structure in the Xianhuayin-treated group. Transmission electronic micro- scopic （TEM） analysis showed that buccal mucosal epithelial cells were morphologically regular in the non-diseased control group. Desmosomes and tonofibrils were reduced and the nucleus was morphologically irregular in the untreated premalignant lesion group. In the Xianhuayin-treated group, the widening intercellular gap was gradually reduced, desmosomes and the cells becoming morphologically regular. No significant difference was observed between the hamsters in NS-treated group and those in the untreated premalignant lesion group. Significant therapeutic efficacy was observed in the group receiving Xianhuayin. Conclusion Xianhuayin is effective in the reversal of DMBA-induced premalignant lesions in the buccal pouch of golden hamsters.
文摘AIM:To explore the model of methotrexate(MTX)-induced mic e oral mucosa lesions METHOD:A total of 282 mice were randomly d ivided into seven groups as follows:control,MTX10,20,30,40,50,75 mg/ml The physiological saline solution (05 ml) was ip injecte d into mice in control group The dose of MTX (05 ml) was ip injected int o mic e in other groups only once During 10 consecutive days after administration of MTX,3 mice were sacrificed in each day in all the groups and the patholog ical chang es of oral mucosa were examed under the optical microscope RE SULT: The incidence(45%～63%) and the integral rate(194%～56%) of mice ora l mu cosa lesions in group of intermediate-dose MTX (IDMTX) (20～40 mg/kg) were sign ifi cantly higher than those in other groups The death rate (not more than 5%) in group of IDMTX was lower than that in other groups CONCLUSION:As a result of induction of higher lesion rate and lower death rate of mice oral mucosa by IDMTX (2 0～40 mg/kg),the model of IDMTX-induced oral mucosa lesion could be applie d into clinical or basic
