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百年韩师办学传统与经验
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作者 孟广军 《韩山师范学院学报》 2013年第4期37-42,共6页
1903年建校的韩师是我国第一批、广东省第一所专门培养师资的学校。百年韩师办学传统与经验可以高度概括为四个方面,即:以校训"勤教力学,为人师表"为贯穿始终的办学理念;传承潮汕文化教育的地方特色;与时俱进的科类与专业设置... 1903年建校的韩师是我国第一批、广东省第一所专门培养师资的学校。百年韩师办学传统与经验可以高度概括为四个方面,即:以校训"勤教力学,为人师表"为贯穿始终的办学理念;传承潮汕文化教育的地方特色;与时俱进的科类与专业设置;侨资、社会各界、海外校友的捐资助学。 展开更多
关键词 百年韩师 办学传统与经验 潮汕
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重组pEGFP—hSnu 114质粒的构建及表达
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作者 李晓冬 方建飞 +3 位作者 东莉洁 葛林 何津岩 杨洁 《医学分子生物学杂志》 CAS CSCD 2009年第1期6-8,共3页
目的将hSnu114基因定向连人pEGFP质粒GFP的下游,使hSnu114蛋白可与绿色荧光蛋白在真核细胞内融合表达。从而为进一步研究hSnu114蛋白的功能奠定实验基础。方法本实验已经构建tr1E57R/T—hSnu114和pcDNA3.1(-)-hSnu114重组质粒,想... 目的将hSnu114基因定向连人pEGFP质粒GFP的下游,使hSnu114蛋白可与绿色荧光蛋白在真核细胞内融合表达。从而为进一步研究hSnu114蛋白的功能奠定实验基础。方法本实验已经构建tr1E57R/T—hSnu114和pcDNA3.1(-)-hSnu114重组质粒,想要构建pEGFP—hSnu114重组质粒,需通过引入SalⅠ酶切位点,调整N端起始密码子与绿色荧光蛋白(green fluorescence protein,GFP)基因之间的序列,使其不破坏读码框,融合蛋白正确表达。PCR扩增出hSnu114的第一部份(SalⅠ~MunⅠ基因序列),从hSnu114-peDNA3.1(-)重组质粒回收hSnu114的第二部分(MunI~BamHI片段),定向克隆至T/A载体,构建hSnu114全长(SalI~BamHI)pTZ57R/T重组质粒。采用SalI和BamHI双酶切方法,从重组质粒中获得hSnu114全长,将该基因片段连接入pEGFP质粒。将构建成功的pEGFP—hSnu114质粒,转染人HeLa细胞,荧光显微镜下可观察绿色荧光蛋白表达。进行Western印迹检测。结果①hSnu114Sal1~MunI目的片段获取。②将该pEGFP—hSnu114质粒进行双酶切鉴定可见hSnu114全长片段。③转染重组质粒后可观察到绿色荧光蛋白的表达。④Western印迹可检测到pEGFP—hSnu114融合蛋白:结论①hSnu114全长成功载入pEGFP质粒。②hSnu114蛋白可与绿色荧光蛋白在真核细胞中融合表达。 展开更多
关键词 hsnu114蛋白 重组质粒 融合蛋白
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Construction of daily precipitation series and the observational characteristics of extreme precipitation in Tianjin,China during 1888-2022
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作者 Peng SI Qing-Xiang LI +2 位作者 Xiao-Yang CHEN Min WANG Chuan-Jun LUO 《Advances in Climate Change Research》 SCIE CSCD 2024年第1期52-61,共10页
Given the difficulties in rescuing and ensuring the quality of long-term climate data,current studies on century-scale climate change are usually limited to annual and monthly data,resulting in the poor detection of e... Given the difficulties in rescuing and ensuring the quality of long-term climate data,current studies on century-scale climate change are usually limited to annual and monthly data,resulting in the poor detection of extreme climate events and their changes before 1950.In this study,we reconstructed a daily precipitation series for Tianjin from 15 September 1887 to 31 December 2022 on the basis of the most comprehensive daily precipitation records collected from the Tianjin Meteorological Archive,China,and in reference to the precipitation analysis results based on the datasets developed by the Climatic Research Unit Time-Series version 4.06,Global Precipitation Climatology Centre and University of Delaware along with the application of various homogenisation methods for climate series.Our approach provides a complete and reliable century-long daily precipitation series for the study of regional or local extreme weather and climate events.The reconstructed daily dataset reveals that the annual precipitation amount and R95 intensity in Tianjin during 1888-2022 lack significant trends and have values of 0.74±6.99 and-1.84±3.22 mm per decade,respectively.On the annual and seasonal scales,the precipitation amount and R95 intensity,particularly those in autumn,have increased since the latter half of the 20th century relative to those in 1888-1950.However,the increase in precipitation amount and R95 intensity is relatively limited compared with that in atmospheric water vapour content due to surface warming,indicating the highly sensitive response of extreme precipitation events to warming.In addition,the estimates for the return periods of 5,10,20,50 and 100 years covering 1888-2022,1888-1950 and 1951-2022 depict that the intensity of heavy rain and above magnitude was highest in 1888-1950 and decreased in 1951-2022. 展开更多
关键词 Over a century-long timescale Daily precipitation series Extreme precipitation Return period
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Creating a New Form of Rule of Law Civilization:A Historical Narrative of the Communist Party of China
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作者 Fu Zitang Li Guanghui 《Social Sciences in China》 2023年第3期4-19,共16页
The modernization path of the Chinese rule of law reflects the glorious process of the Communist Party of China(CPC)’s continuous exploration of the rule of law development.It has created a new form of rule of law ci... The modernization path of the Chinese rule of law reflects the glorious process of the Communist Party of China(CPC)’s continuous exploration of the rule of law development.It has created a new form of rule of law civilization for humanity,one that has not only profoundly changed China but is also deeply influencing the world historical process.Under the leadership of the CPC,the Chinese people have achieved the innovation of legal mechanisms,text,and discourse in their century-long endeavor for the rule of law.This highlights the distinctive features of the Chinese rule of law civilization.The innovation of legal mechanisms is the foundation of the modernization of the Chinese rule of law,indicating that through the Party’s century-long endeavor for the rule of law,a legal leadership mechanism has been formed,in which the Party exercises overall leadership and coordinates the efforts of all sides;the innovation of legal text is the manifestation of modernization of the Chinese rule of law,indicating that through the Party’s century-long endeavor for the rule of law,the socialist legal system with Chinese characteristics is gradually moving toward a new stage of codification in which the promulgation and implementation of the Civil Code of the People’s Republic of China is a milestone;and the innovation of legal discourse is the core of modernization of the Chinese rule of law,indicating that through the Party’s century-long endeavor for the rule of law,the continuous refinement of the Chinese legal discourse system is playing a key role in providing theoretical support and legal reasoning. 展开更多
关键词 20th CPC National Congress Xi Jinping Thought on the Rule of Law century-long endeavor for the rule of law a new form of rule of law civilization modernizationof theChineseruleoflaw
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