BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth fa...BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth factorl65(VEGF165) and observe its expression in vascular smoothmuscles (VSMCs).METHODS: The primers were designed and synthesizedaccording to the gene sequences of human VEGF165. TheVEGF165 gene was obtained from umbilic artery tissue bythe method of RT-PCR, then it was cloned to eukaryoticexpression plasmid pBudCE4.1 by recombination strategy.The eukaryotic expression plasmid named pBudCE4.1/VEGF165 was identified by restriction enzyme digestion,and was sequenced. The pBudCE4.1/VEGF165 was trans-fected into VSMCs by using lipofection. The VEGF165 ex-pression of mRNA and protein was detected by RT-PCRand Western blot respectively.RESULTS: VEGF165 was shown about 576bp by RT-PCR.Sequencing revealed the amplified VEGF165 gene was iden-tical with that in the GeneBank. Restrictive enzyme (HindBam HI) digestion analysis showed that recombinantexpression plasmid pBudCE4. l/tVEGF165 had been con-structed successfully. The expression of VEGF165 at mRNAand protein levels in the transformed VSMCs had beendemonstrated by RT-PCR and Western blot.CONCLUSIONS: The recombinant eukaryotic expressionplasmid pBudCE4.1/VEGF165 has been successfully con-structed and expressed in transformed VSMCs. The presentstudy has laid a foundation for VEGF165 gene therapy ofvascular stenosis in the transplant organ.展开更多
The United States Congress set a benchmark in US anti-human trafficking policy with the Trafficking Victims Protection Act of 2000 （TVPA 2000J. The TVPA 2000 and its subsequent reauthorizations in 2003, 2005, and 200...The United States Congress set a benchmark in US anti-human trafficking policy with the Trafficking Victims Protection Act of 2000 （TVPA 2000J. The TVPA 2000 and its subsequent reauthorizations in 2003, 2005, and 2008 were put forth to increase the scope, focus, and effectiveness of US efforts to combat human trafficking. In-depth analyses of each reauthorization provide an understanding of the aims of the TVPA. The evolution of the TVPA reauthorizations dearly demonstrates that the US is committed to thwarting human trafficking both domestically and internationally. The analyses examine strengths and weaknesses of the legislation and provide insight as to how the TVPA reached its current state as the William Wilberforce Trafficking Victims Protection Reauthorization Act of 2008 （TVPRA 2008）. Drawing from the evolution of TVPA legislation, the future of the human trafficking phenomenon and legislation is discussed.展开更多
The theme of trafficking of human beings treats one of the most severe and dangerous criminal phenomena of the organized crime in Albania. The objects of this paper are： human trafficking; ways of trafficking; risk o...The theme of trafficking of human beings treats one of the most severe and dangerous criminal phenomena of the organized crime in Albania. The objects of this paper are： human trafficking; ways of trafficking; risk of Albanian society; and investment of the criminal benefits deriving from trafficking in legal economic activities, it is of great importance for the treatment of proactive investigations to the trafficking of human beings. Another important aspect of the trafficking investigations is the investigation of criminal assets and the way how they are used in the economy of the country. In this paper, we stress that the investigation of criminal assets benefited by the crime of trafficking human beings as well as other wealth financed by these assets is important as the investigation of the criminal activity of trafficking itself. Also, we aim to evaluate the necessity of acting of all law enforcement agencies and other state institutions in order to be successful in the fight against the crime of trafficking of human beings. At the end of this paper, we do treat the necessity and the ways of collaboration of law enforcement agencies, NGOs, and structures of local government for rehabilitation of the victims of trafficking and their integration in the social life.展开更多
To regulate human organ transplant, guarantee the quality and safety of the operation, protect the health of the people, the Health Ministry of China issued Regulations governing Clinical Application of Human Organ Tr...To regulate human organ transplant, guarantee the quality and safety of the operation, protect the health of the people, the Health Ministry of China issued Regulations governing Clinical Application of Human Organ Transplant on March 27, 2006, which is to be put into effect on the coming July 1. The regulations contain 5 chapters and 47 articles including general principles, registration of diagnosis and treatment, clinical management, supervision, and supplementary items.展开更多
Human leukocyte antigen(HLA)compatibility is very important for successful transplantation of solid organs.In this paper,we focused on the humoral arm of immunity in the clinical setting of organ transplantation:how H...Human leukocyte antigen(HLA)compatibility is very important for successful transplantation of solid organs.In this paper,we focused on the humoral arm of immunity in the clinical setting of organ transplantation:how HLA antibodies develop,how they can be detected,and what they can do to injure organ transplants.Specifically,we explore the technical perspectives of detecting donor-specific antibodies(DSA)in HLA laboratories,and use real-life clinical cases to explain the principles.Currently there are many tools in our HLA antibody detection toolbox:conventional cytotoxicity cross match,flow cross match,and solid phase assays using beads conjugated with single or multiple HLA antigens.Single antigen bead(SAB)assay is the most sensitive tool available for detecting HLA antibodies and assessing the immunological risk for organ transplant.However,there are intrinsic limitations to solid-phase assays and they are prone to both false negativity and importantly,false positivity.Denatured antigens on single antigen beads might be the most prominent source of false positive reactivity,and may have been underestimated by many HLA experts.No single assay is perfect and therefore multiple methods,including the less sensitive assays,should be employed to determine the clinical relevance of detected HLA antibodies.Thoughtful process,including knowledge of HLA systems,cross reactivity,epitopes,and the patient's clinical history should be employed to correctly interpret data.The clinical team should work closely with HLA laboratories to ensure accurate interpretation of information and optimal management of patients before and after organ transplantation.展开更多
基金This study was supported by grants from the 973 National Basic ResearchProgram of China ( 2003CB515501 ) and the National Natural ScienceFoundation of China (No. 30270514).
文摘BACKGROUND: The highly specific vascular endothelialgrowth factor (VEGF) induces the growth of vascular en-dothelial cell. This study was to construct the eukaryoticexpression plasmid of vascular endothelial growth factorl65(VEGF165) and observe its expression in vascular smoothmuscles (VSMCs).METHODS: The primers were designed and synthesizedaccording to the gene sequences of human VEGF165. TheVEGF165 gene was obtained from umbilic artery tissue bythe method of RT-PCR, then it was cloned to eukaryoticexpression plasmid pBudCE4.1 by recombination strategy.The eukaryotic expression plasmid named pBudCE4.1/VEGF165 was identified by restriction enzyme digestion,and was sequenced. The pBudCE4.1/VEGF165 was trans-fected into VSMCs by using lipofection. The VEGF165 ex-pression of mRNA and protein was detected by RT-PCRand Western blot respectively.RESULTS: VEGF165 was shown about 576bp by RT-PCR.Sequencing revealed the amplified VEGF165 gene was iden-tical with that in the GeneBank. Restrictive enzyme (HindBam HI) digestion analysis showed that recombinantexpression plasmid pBudCE4. l/tVEGF165 had been con-structed successfully. The expression of VEGF165 at mRNAand protein levels in the transformed VSMCs had beendemonstrated by RT-PCR and Western blot.CONCLUSIONS: The recombinant eukaryotic expressionplasmid pBudCE4.1/VEGF165 has been successfully con-structed and expressed in transformed VSMCs. The presentstudy has laid a foundation for VEGF165 gene therapy ofvascular stenosis in the transplant organ.
文摘The United States Congress set a benchmark in US anti-human trafficking policy with the Trafficking Victims Protection Act of 2000 （TVPA 2000J. The TVPA 2000 and its subsequent reauthorizations in 2003, 2005, and 2008 were put forth to increase the scope, focus, and effectiveness of US efforts to combat human trafficking. In-depth analyses of each reauthorization provide an understanding of the aims of the TVPA. The evolution of the TVPA reauthorizations dearly demonstrates that the US is committed to thwarting human trafficking both domestically and internationally. The analyses examine strengths and weaknesses of the legislation and provide insight as to how the TVPA reached its current state as the William Wilberforce Trafficking Victims Protection Reauthorization Act of 2008 （TVPRA 2008）. Drawing from the evolution of TVPA legislation, the future of the human trafficking phenomenon and legislation is discussed.
文摘The theme of trafficking of human beings treats one of the most severe and dangerous criminal phenomena of the organized crime in Albania. The objects of this paper are： human trafficking; ways of trafficking; risk of Albanian society; and investment of the criminal benefits deriving from trafficking in legal economic activities, it is of great importance for the treatment of proactive investigations to the trafficking of human beings. Another important aspect of the trafficking investigations is the investigation of criminal assets and the way how they are used in the economy of the country. In this paper, we stress that the investigation of criminal assets benefited by the crime of trafficking human beings as well as other wealth financed by these assets is important as the investigation of the criminal activity of trafficking itself. Also, we aim to evaluate the necessity of acting of all law enforcement agencies and other state institutions in order to be successful in the fight against the crime of trafficking of human beings. At the end of this paper, we do treat the necessity and the ways of collaboration of law enforcement agencies, NGOs, and structures of local government for rehabilitation of the victims of trafficking and their integration in the social life.
文摘To regulate human organ transplant, guarantee the quality and safety of the operation, protect the health of the people, the Health Ministry of China issued Regulations governing Clinical Application of Human Organ Transplant on March 27, 2006, which is to be put into effect on the coming July 1. The regulations contain 5 chapters and 47 articles including general principles, registration of diagnosis and treatment, clinical management, supervision, and supplementary items.
文摘Human leukocyte antigen(HLA)compatibility is very important for successful transplantation of solid organs.In this paper,we focused on the humoral arm of immunity in the clinical setting of organ transplantation:how HLA antibodies develop,how they can be detected,and what they can do to injure organ transplants.Specifically,we explore the technical perspectives of detecting donor-specific antibodies(DSA)in HLA laboratories,and use real-life clinical cases to explain the principles.Currently there are many tools in our HLA antibody detection toolbox:conventional cytotoxicity cross match,flow cross match,and solid phase assays using beads conjugated with single or multiple HLA antigens.Single antigen bead(SAB)assay is the most sensitive tool available for detecting HLA antibodies and assessing the immunological risk for organ transplant.However,there are intrinsic limitations to solid-phase assays and they are prone to both false negativity and importantly,false positivity.Denatured antigens on single antigen beads might be the most prominent source of false positive reactivity,and may have been underestimated by many HLA experts.No single assay is perfect and therefore multiple methods,including the less sensitive assays,should be employed to determine the clinical relevance of detected HLA antibodies.Thoughtful process,including knowledge of HLA systems,cross reactivity,epitopes,and the patient's clinical history should be employed to correctly interpret data.The clinical team should work closely with HLA laboratories to ensure accurate interpretation of information and optimal management of patients before and after organ transplantation.
