The Influence of Advanced Paternal Age on Sperm Chromatin Integrity and Early Embryo Morphological Development during ICSI

The study determined the impact of advanced male ageing (≥50 years) on sperm chromatin integrity and early embryo morphological development in intra-cytoplasmic sperm injection (ICSI) technique cycles. Six hundred subfertile men were age-grouped;X1 (50 to 59 years), X2 (60 to 69), and X3 (≥70), were compared with 600 fertile males of known fertility (Y, age 25 - 35 years). Oocytes from 254 women, aged ≤ 30 years, were included. Sperm were analyzed using acridine orange fluorescence test (AOT) and categorized: “low”, “inter-mediate” and “high” damage. After ICSI, embryos were evaluated and categorized as “good”, “fair” or “poor” quality. Embryonic morphological development was assessed at three stages: fertilization, early and late paternal effect. The AOT results were: X1: low = 29, intermediate = 53 and high = 118;X2: low = 11, intermediate = 42 and high = 147;X3: low = 8, intermediate = 24 and high = 168;Y: Low = 486, intermediate = 71 and high = 43. The fertilization rate was: X1, 329/350 (93.7%);X2, 298/350 (85.1%);X3, 225/350 (64.1%) and, Y, 350/350 (100%). Associations between increasing age and sperm chromatin damage (χ2 (723.249, 6) p < 0.0001), increasing age and inability to fertilize (χ2 (210.990, 3) p < 0.0001) were observed. Associated with increasing age was the significant proportion of morphologically poor quality embryos over the five days after fertilization. Male age ≥ 50 years, is highly associated with abnormal sperm chromatin organization, an inability to adequately fertilize with ICSI methodology, an increase in the number of poor quality embryos and, a corresponding decrease in the number of good quality embryos five days after fertilization.

Effects of seawater acidification on the early development of sea urchin Glyptocidaris crenularis

In this study, we evaluated the effects of CO_2-induced seawater acidification on fertilization, embryogenesis and early larval development in the sea urchin Glyptocidaris crenularis, that inhabits subtidal coastal areas in northern China. The range in seawater p H used in experiments was based on the projections of the Intergovernmental Panel on Climate Change(IPCC), to the year 2100. A natural seawater treatment(p H_(nbs) =7.98±0.03) and three laboratory-controlled acidified treatments(OA_1, ΔpH_(nbs) =-0.3 units; OA_2, ΔpH_(nbs) =-0.4 units; OA_3, ΔpH_(nbs) =-0.5 units) were used in experiments. Results show that:(1) there was a negative effect of seawater acidification on fertilization and on the percentage of abnormal fertilized eggs;(2) the size of early cleavage stage embryos decreased in a dose-dependent manner with decreasing p H;(3) both the hatching rate of blastulae and the survival rate of four-armed pluteus larvae decreased as pH declined;(4) larval abnormalities including asymmetrical development, changes in the length of skeletal elements, and corroded spicules were observed in all seawater acidified-treatments compared with the control. These data indicate that seawater acidification has a negative impact on the early development of G. crenularis, and supports the hypothesis that the response of echinoderms to ocean acidification(OA) varies among species. Further research is required to clarify the specific cellular mechanisms involved.

Radiation dose effects on the morphological development of M_(1) generation pea(Pisum sativum)

We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose range of 0.51-9.27 Gy,with the increase in neutron-absorbed dose,the morphological development parameters of M_(1) generation peas at the initial seedling stage showed an obvious trend with three fluctuations.With the development of pea,this trend gradually weakened.Further analysis and verification showed that the main trend in the M_(1) generation of pea seeds was an inhibitory effect induced by neutron irradiation and there was a good linear correlation between the inhibitory effect and neutron absorption dose We successfully demonstrated the background removal of mutant plants and defined morphological developmen parameters for peas that match the overall development of plants.Our results will positively impact neutron mutation breeding and automatic agriculture.

Effect of Different Sowing Dates and Densities on Individual Morphological Development of Super Short-season Insect-resistant Cotton

[Objective] The paper was to explore the effect of different sowing dates and densities on individual morphological development of super short-season insect-resistant cotton,confirm their effects on vegetative and reproductive growth of cotton,so as to provide theoretical and practical guidance for sowing date and density management of cotton planting in Jidong cotton growing region in Yellow River Basin.[Method] With super short-season insect-resistant cotton＂546＂as materials,the effects of different sowing dates（sowing dateⅠ：May 20;sowing dateⅡ：June 2;sowing date Ⅲ：June 14）and densities（low density：120 000 plants/hm2;middle density：150 000 plants/hm2;high density：180 000 plants/hm2）on individual morphological development of super short-season insect-resistant cotton were explored.[Result] Different sowing dates and density treatments significantly affected the individual morphological development of super short-season insect-resistant cotton＂546＂.The effectiveness of sowing date was higher than the effectiveness of density,and the effectiveness of sowing date on development of number of individual fruit branches was higher than that on plant height and stem diameter.[Conclusion] The regulation of sowing date and density during the cultivation process of super short-season insect-resistant cotton ＂546＂ in Jidong cotton growing region in Yellow River Basin could effectively promote vegetative and reproductive growth of cotton,strengthening its production base.

Simulation of Morphological Development of Soil Cracks in Yuanmou Dry-hot Valley Region, Southwest China

Soil cracking is an important process influencing water and solutes transport in the Yuanmou Dry-hot Valley region of Southwest China. Studying the morphological development of soil cracks helps to further reveal the close relationship between the soil cracking process and water movement in such semi-arid regions. Here we report regular changes on surface morphology of soil cracks with decreasing water in four different soils (Typ-Ustic Ferrisols,Ver-Ustic Ferrisols,Tru-Ustic Vertisols and Typ-Ustic Vertisols) through simulation experiments. Our results indicate the following: 1) Different soils ultimately have different development degrees of soil cracks,according to their various values of crack area density. Soil cracks in Typ-Ustic Ferrisols can only develop to the feeble degree,while those in the other three soils are capable of developing into the intensive degree,and even into the extremely intensive degree. 2) Soil crack complexity,as expressed by the value of the area-weighted mean of crack fractal dimension (AWMFRAC),is found to continuously decrease as a whole through the whole cracking process in all the studied soils. 3) Soil crack connectivity shows a uniform trend in the studied soils,that is to say,connectivity gradually increases with soil crack development.

The early life immune dynamics and cellular drivers at single-cell resolution in lamb forestomachs and abomasum

Background Four-chambered stomach including the forestomachs(rumen,reticulum,and omasum)and abomasum allows ruminants convert plant fiber into high-quality animal products.The early development of this four-chambered stomach is crucial for the health and well-being of young ruminants,especially the immune development.However,the dynamics of immune development are poorly understood.Results We investigated the early gene expression patterns across the four-chambered stomach in Hu sheep,at 5,10,15,and 25 days of age.We found that forestomachs share similar gene expression patterns,all four stomachs underwent widespread activation of both innate and adaptive immune responses from d 5 to 25,whereas the metabolic function were significantly downregulated with age.We constructed a cell landscape of the four-chambered stomach using single-cell sequencing.Integrating transcriptomic and single-cell transcriptomic analyses revealed that the immune-associated module hub genes were highly expressed in T cells,monocytes and macrophages,as well as the defense-associated module hub genes were highly expressed in endothelial cells in the four-stomach tissues.Moreover,the non-immune cells such as epithelial cells play key roles in immune maturation.Cell communication analysis predicted that in addition to immune cells,non-immune cells recruit immune cells through macrophage migration inhibitory factor signaling in the forestomachs.Conclusions Our results demonstrate that the immune and defense responses of four stomachs are quickly developing with age in lamb's early life.We also identified the gene expression patterns and functional cells associated with immune development.Additionally,we identified some key receptors and signaling involved in immune regulation.These results help to understand the early life immune development at single-cell resolution,which has implications to develop nutritional manipulation and health management strategies based on specific targets including key receptors and signaling pathways.

Early Intervention Facilitates Neuropsychological Development in Children with Autism and Attention Deficit Hyperactivity Disorder

Objective:This study aims to investigate the impact of early intervention on neuropsychological development in children with autism and attention deficit hyperactivity disorder(ADHD),providing effective intervention strategies for clinical practice.Methods:A total of 130 children with autism and ADHD who visited the hospital between June 2023 and June 2024 were selected as study subjects and randomly divided into an intervention group and a control group,with 65 children in each group.The intervention group received a one-year early comprehensive intervention,including behavioral therapy,cognitive training,and family guidance,while the control group only received routine medical care.The neuropsychological development assessment scale was used to evaluate both groups before and after the intervention to compare changes in their neuropsychological development levels.Results:Children in the intervention group showed significant improvements in cognitive function,social skills,language ability,and attention concentration,with an average improvement score of 23.5 points.Children in the control group did not show significant improvements in these areas,with an average improvement score of only 5.8 points.The difference between the two groups was statistically significant(P<0.05).Conclusion:Early comprehensive intervention has a significant promoting effect on the neuropsychological development of children with autism and ADHD.Targeted behavioral therapy,cognitive training,and family guidance can effectively enhance children’s cognitive,social,language,and attention abilities,laying a solid foundation for their future overall development.Therefore,it is recommended to actively promote and apply early intervention strategies in clinical practice.

THE STUDY OF EARLY DEVELOPMENT AND EVOLUTION OF Torrentophryne aspinia

Torrentophryne aspinia is a new kind of toads discovered in China recently, it lives in the forest floor in West Yunnan, the most important things for this kind of species is that they have a kind of torrent-adapted tadpoles which have abdominal suckers and can adhere to the surfaces of rocks in moving waters. In this article, we described the forming procedures of abdominal sucker and some breeding behaviors, habitats and habits. After comparing with those of other toads, it is confirmed that Torrentophryne should be a new valid genus, it is derived from ancestor toads because of adaptation to the torrent habitats, by the forming of torrent -adapted organ-abdominal sucker in the early development.

Enantiornithine Bird with Diapsidian Skull and Its Dental Development in the Early Cretaceous in Liaoning, China

A Iarge number of enantiornithine birds are discovered from the Early Cretaceous Jiufutang Formation in western Liaoning, China. They are all small-sized birds with a few small teeth. The enantiornithine bird from the Jiufutang Formation in the Shangheshou area, Chaoyang, Liaoning Province reported in this paper is the largest individual known in all enantiornithine birds of the Early Cretaceous. However, its teeth possess a feature of pseudoheterodont. Some different development stages of the new teeth substitute the earlier stages and the stages of development are preserved in this specimen. This development pattern is similar to that of Archaeopteryx and alligator but not dinosaur. A well-developed postorbital was also preserved in the skull, which was a diapsidian skull like that of Confuciusornis. Additionally, the distinctive preservation of its prefrontal distinguishes it from other enantiornithine birds of the Early Cretaceous.

The role of Dby mRNA in early development of male mouse zygotes

Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte during fertilization by reverse transcription-polymerase chain reaction even though no DBY protein expression is detected. The cellular location ofDby mRNA is seen in the post-acrosome region, and it comprises nearly half of the mouse spermatozoa in in situ hybridization. In contrast, transcripts of the control gene, Smcy, are not detected in capacitated mouse spermatozoa, although the H-Y antigen encoded by Smcy is expressed on the surface of the spermatozoa. In our microinjection experiment, the zygotic development rate of the as-Dby male pronucleus injection group was significantly lower than that of the as-Smcy male pronucleus injection group （35.9% vs. 95%, P = 0.001） and the as-Dby female pronucleus injection group （35.9% vs. 93.8%, P = 0.001）. The rate of male-developed zygotes was also lower than that of the as-Smcy male pronucleus injection group （17.4% vs. 57.9%, P = 0.002） and the as-Dby female pronucleus injection group （17.4% vs. 54.1%, P = 0.002）. Thus, we conclude that Dby mRNA is selectively retained in capaci- tated mouse spermatozoa, and it has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction.

The response of the early developmental stages of Laminaria japonica to enhanced ultraviolet-B radiation

The responses of the early development of Laminaria japonica collected from Kiaochow Bay in China to enhanced ultraviolet-B radiation (UV-B, 280—320 nm) were studied in the laboratory. The low UV-B ra-diations (11.7—23.4 J·m-2·d-1) had no significant effects on zoospores attachment, but when the UV-B dose > 35.1 J·m-2·d-1 the attachment decreased significantly compared with the control. Germination of embryospores was >93% under the low (11.7—35.1 J·m-2·d-1) doses, and in the range of 78.5%—88.5% under the high (46.8—70.2 J·m-2·d-1) UV-B doses, indicating a significant radiation effect. Under the higher UV-B exposure (35.1—70.2 J·m-2·d-1), all of the few gametophytes formed from embryospores died 120 h post-release. After exposure to the low UV-B radiation (11.7—23.4 J·m-2·d-1), the formation of sporophytes decreased and the female gametophyte clones increased compared with the control. However, the sex ratio and the relative growth of female gametophytes/sporophytes had not signifi-cantly changed. According to the results, enhanced UV-B radiation has a significant effect on the early development of L. japonica under laboratory conditions, suggesting that the UV-B radiation could not be overlooked as one of the important environmental factors influencing the ontogeny of macroalgae living in marine ecosystems.

Ontogenetic development in the morphology and behavior of loach(Misgurnus anguillicaudatus) during early life stages

Loach （Misgurnus anguillicaudatus） is a commercially important fish in China and an ideal aquaculture species. However, culturists experience high larval and juvenile mortality during mass production. To provide insight into ways to improve larviculture techniques, we describe the morphological characteristics and behavior of loach during the larval and early juvenile stages. Yolksae larvae ranged from 2.8 to 4.0 mm body length （BL） between days 0 to 4; preflexion larvae ranged from 3.6 to 5.5 mm BL between days 4 to 6; flexion larvae ranged from 4.8 to 8.1 mm BL between days 5 and 14; and post-flexion larvae ranged from 7. l to 15.7 mm BL between days 11 to 27; the minimum length and age of juveniles was 14.1 mm BL and 23 d, respectively. Loach are demersal from hatch through to the early juvenile stages. A suite of morphological characteristics （e.g., external gill filament and ventral mouth opening） and behavioral traits have developed to adapt to demersal living. We observed positive allometric growth in eye diameter, head length, head height, and pectoral fin length during the early larval stages, reflecting the priorities in the development of the organs essential for survival. Our results provide a basis for developing techniques to improve the survival of larval and juvenile loach during mass production.

Antibodies against the C-terminal peptide of rabbit oviductin inhibit mouse early embryo development to pass 2-cell stage

A full-length rabbit oviductin cDNA(1909bp) was cloned. It consists of a 5’-UTR of 52bp, an open reading frame (ORF) of 1374bp and a 3’-UTR of 483bp and has more than 80% homology with that of other mammal oviductins. N-terminal peptide (NTP) (384 residues) and C-terminal peptide (CTP) (73 residues) of deduced protein precursor has about 80% and 50% identity with that of other mammals respectively. Fusion proteins GST-NTP 368(1R-368N)and GST-CTP73 (369F-441A) were expressed and purified. NH2-terminal of CTP sequencing reveals that the purified protein is consistent with the deduced one. In order to study the function of NTP and CTP the mouse anti-NTP and rabbit anti-CTP antisera were prepared. Tissue-specific (skeleton muscle, oviduct, uterus, ovary, liver, heart and brain) analysis indicated that rabbit oviductin was only found in oviduct. The conditioned medium derived from the rabbit oviduct mucosa epithelial cells has a function of overcoming the early embryonic development block of Kunming mous e cultured in vitro. Anti-CTP antiserum could totally inhibit the early embryo development at 2-cell stage cultured in the conditioned culture medium, but anti-NTP antiserum couldn’t. There was a positive relationship between the ratio of early embryos at development block and the dosage of anti-CTP antiserum added in the conditioned culture medium. These results suggest that oviductin has a function not only on fertilization, but also on the release of early embryonic development block, and the later function domain of rabbit oviductin may be situate in its C-terminal.

Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole(Cynoglossus semilaevis)

Differential expression of genes is crucial to growth and development of fish. To select the appropriate genes for gene normalization during Cynoglossus semilaevis early developmental process, eight candidate reference genes （ACTB, B2M, EF1A, GADPH, RPL7, TUBA, UBCE and 18S） were tested for their adequacy by using quantitative real-time PCR. The results showed that the expression of all the examined genes exhibited tissue dependent variations in the mature C. semilaevis. EFIA was listed as the most stable reference among the 14 tissues by RefFinder. Furthermore, the recommended comprehensive ranking of the stability determined by RefFinder showed that 18S was the most stable gene during the early developmental stages （from oosphere to 90 days old） in this study. However, when divided the Ct value data of the above mentioned early developmental stages into two separate periods （embryo and post-hatching periods）, TUBA and 18S represented the most stable references of these two developmental periods, respectively. Consequently, the reference gene should be carefully and accurately chosen even for studies of the same species at various developmental processes. The relevant data may help in selecting appropriate reference genes for mRNA expression analysis, and is of great value in the studies of fish growth and development.

Early Childhood Development (0 - 6 Years Old) from Healthy to Pathologic: A Review of the Literature

Early childhood development involves an appropriate global intervention concerning the different areas of development such as: physical, cognitive, language and socio-emotional [1]. Especially during the first six years of life in an adequate environment rich in exchange and interaction. We will present in this article a review of the literature concerning normal early childhood development, focus firstly in normal development’s steps, the importance of first experiences and first aid with a stable and caring attachment figure. second, we will describe the various developmental disorders, occurring during this age group which extends from birth to 6 years and based on the DSM5 classification, namely that developmental delay may concern only one aspect of development (e.g. language delay or motor delay specifically) or concern the different aspects of development (overall development delay). The dynamics of development is also important to clarify because schematically we contrast the pathologies fixed to progressive pathologies (where we find the concepts of stagnation or regression in the development of the child). We will discuss in the third chapter, the interest of early detection as well as the multiple clinical and psychometric diagnostic tools, which aim to detect any delay or disorder in order to initiate appropriate care and minimize the long-term consequences.

A dynamic regulation of nitrogen on floret primordia development in wheat

Nitrogen(N)fertilization is critical for spike and floret development,which affects the number of fertile florets per spike(NFFs).However,the physiological regulation of the floret development process by N fertilization is largely unknown.A high temporal-resolution investigation of floret primordia number and morphology,dry matter,and N availability was conducted under three N fertilization levels:0(N0),120(N1)and 240(N2)kg ha^(−1).Interestingly,fertile florets at anthesis stage were determined by those floret primordia with meiotic ability at booting stage:meiotic ability was a threshold that predicted whether a floret primordium became fertile or abortive florets.Because the developmental rate of the 4th floret primordium in the central spikelet was accelerated and then they acquired meiotic ability,the NFFs increased gradually as N application increased,but the increase range decreased under N2.There were no differences in spike N concentration among treatments,but leaf N concentration was increased in the N1 and N2 treatments.Correspondingly,dry matter accumulation and N content of the leaf and spike in the N1 and N2 treatments was increased as compared to N0.Clearly,optimal N fertilization increased leaf N availability and transport of assimilates to spikes,and allowed more floret primordia to acquire meiotic ability and become fertile florets,finally increasing NFFs.There was no difference in leaf N concentration between N1 and N2 treatment,whereas soil N concentration at 0–60 cm soil layers was higher in N2 than in N1 treatment,implying that there was still some N fertilization that remained unused.Therefore,improving the leaf’s ability to further use N fertilizer is vital for greater NFFs.

Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

Objective:To investigate the effects of coenzyme Q10(CoQ10)supplementation on post-vitrification embryo development and gross morphology.Methods:Balb/c mouse embryos were cultured in potassium simplex optimised medium(KSOM)with varying CoQ10 concentrations[0(control),20,40,and 60μM].The most effective CoQ10 concentration(40μM)was selected for subsequent post-vitrification morphology study.Embryos were randomly divided into four groups:Group A(non-vitrified without CoQ10),Group B(non-vitrified with CoQ10),Group C(vitrified without CoQ10),and Group D(vitrified with CoQ10),followed by vitrification at the 8-cell stage.Survival rates and development until the blastocyst stage were evaluated through morphological examinations using ASEBIR's system,distinguishing normal and abnormal embryos.Results:Supplementation of 40μM CoQ10 significantly increased blastocyst formation(95%)compared to the control group(92%),20μM(62%),and 60μM(56%)(P<0.001).Following vitrification,Group D exhibited a significant increase in blastocyst formation(92%)compared to Group C(82%)(P<0.05).Morphological assessments indicated superior embryo quality in Group B over Group D during the cleavage stage,morula,and blastocyst(P<0.05).Conclusions:CoQ10 supplementation exhibits promising potential to enhance preimplantation embryo development,increase blastocyst formation rates,and improve embryo quality post-vitrification.This offers a promising approach to mitigate oxidative stress on embryos,potentially improving overall assisted reproductive technology outcomes.

Morphological and histological changes in the brains of turbot(Scophthalmus maximus)with gonadal development

The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and North China.Previous research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm production.However,the morphology and histology of the organs in the HPG axis have not been studied.In this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal development.The results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory bulbs.The telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal portion.The torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal portion.The actual total brain volume in mature males was significantly greater(p<0.05)than that of females with gonadal development.Notably,the pituitary volume in male turbot significantly increased(p<0.05)from immature to mature stage,but this difference did not occur in females.The data together illustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes.

The impacts of timing and insecticides at early stage of late rice on the population development of brown plant hopper(BPH)

Field experiments were carried out in northern Zhejiang Province. Three insecticides (methamidophos,Shachongshuang and triazophos) were used to control the rice leaffolder Cnaphalocrocis medinalis and rice striped stemborer Chilo suppressalis in rice fields during 18—27 days after transplantating. The impacts of timing and insecticides on the population development of brown planthopper Nilaparavata lugens was surveyed by direct counting on the plants or by tapping method.