The Method for Measuring the Loop Resistance and the Temperature Rise Calculation of GIS Conductor Pole

This paper studies the method for measuring the loop resistance of GIS conductor pole based on the super capacitor producing impulse current up to several thousand amperes. This method overcomes the limitations of conventional diagnostic method. Typical GIS conductor poles are chosen. Based on FEA and lab tests, the effect of different forms of current and contact condition, relationship between the temperature of contact and the loop resistance is researched. In full- scale testing under realistic operating conditions on the new 220 kV GIS using prototype instrumentation a very good sensitivity in an early stage was obtained.

Upgrade to the front-end electronics of the BESⅢ muon identification system

Resistive Plate Chambers(RPCs) built from a new type of Bakelite developed at Institute of High Energy Physics(IHEP),Chinese Academy of Sciences have been used in the BESⅢ Muon identification system for several years without linseed oil coating,but characteristic aging performances were observed.To adapt to the RPCs in the aging state,the front-end electronics have been upgraded by enhancing the front-end protection,improving the threshold setting circuit,and separating power supplies of the comparator and the field programmable gate array(FPGA).Improvements in system stability,front-end protection and threshold consistency have been achieved.In this paper,the system upgrade and the test results are described in detail.

Improving the Performance of Urban Waste Management Systems in the Context of a Closed-Loop Supply Chain

The saving of non-renewable energies, as well as the reduction of emissions into the environment, are two crucial objectives of industrial production. The recovery of post-consumer products associated with the use of end-of-life products is part of a context of optimization of these objectives. This recovery maximizes the use of resources from end-of-life products in a circular logic while recording the environmental footprint. This study considers a recycling strategy adapted to the need and urgency to reduce greenhouse gas emissions caused by global warming. The proposed model aims to optimize the profits of the circular manufacturing strategies while minimizing operational costs (collection, sorting, recycling), transport, GHG emissions and recycling. In this paper, a compromise between the gains of CM and the costs associated with it was studied. The robustness of the designed model was tested using a case study based on real-world scenarios. A sensitivity analysis was carried out to study the impact of the emission cost on the overall objective, considering the two options currently offered to industries. The obtained results support companies to take the ecological aspect into account and integrate sustainable development into their strategic axes for their logistics supply chains.

Destructive field tests on mobilization of end resistance of cast-in-situ bored piles

A series of well-designed full-scale destructive load tests were conducted on six bored piles to investigate the influence of loose debris at the pile tip on end resistance. The results show that soft debris below the pile tip will weaken the mobilization of end resistance. The ultimate tip resistance of post-grouted pile is 2.05 times that of the pile without post-grouting and the ultimate tip resistance in the second load cycle is 2.31 times that of pile in the first load cycle. The relationship between unit end resistance and displacement follows a linear model and a bilinear mode in the first load cycle and the second load cycle, respectively, whereas the unit end resistance-displacement response of post-grouted bored pile can be simulated using a bilinear mode. The critical end resistance ranges between 2 000 kN and 3 000 kN and the critical displacement ranges between 2.5 mm and 4.5 mm in the bilinear mode. As for piles rested on moderately-weathered peliticsiltstone, the socketed length has no effect on the end resistance because of the existence of loose debris.

Study of the Bearing Capacity at the Variable Cross-Section of A Riser- Surface Casing Composite Pile

Reducing the cost of offshore platform construction is an urgent issue for marginal oilfield development.The offshore oil well structure includes a riser and a surface casing.The riser,surface casing and oil well cement can be considered special variable cross-section piles.Replacing or partially replacing the steel pipe pile foundation with a variable cross-section pile to provide the required bearing capacity for an offshore oil platform can reduce the cost of foundation construction and improve the economic efficiency of production.In this paper,the finite element analysis method is used to investigate the variable cross-section bearing mode of composite piles composed of a riser and a surface casing in saturated clay under a vertical load.The calculation formula of the bearing capacity at the variable section is derived based on the theory of spherical cavity expansion,the influencing factors of the bearing capacity coefficient N_(c) are revealed,and the calculation method of N_(c) is proposed.By comparing the calculation results with the results of the centrifuge test,the accuracy and applicability of the calculation method are verified.The results show that the riser composite pile has a rigid core in the soil under the variable cross-section,which increases the bearing capacity at the variable cross-section.

ITO thin films prepared by electron beam evaporation with End-Hall ion source assisted without heating to the substrate

ITO （indium oxide doped with tin） thin films were deposited on glass substrates by using ITO pellet with a composition of w（In2O3）=90% and w（SnO2）=10% by electron beam evaporated with End-Hall ion source assisted without extra heating. The rate of deposition and flow rate of oxygen were measured and changed to obtain the best properties of ITO thin films. Furthermore, the post annealing process was done in vacuum at different annealing temperatures for 2 h and at 400℃ for different keeping time, respectively. The relation between optical, electrical properties and structure was discussed in detail.

Current concepts and future strategies in the antimicrobial therapy of emerging Gram-positive spontaneous bacterial peritonitis

Spontaneous bacterial peritonitis(SBP) is the most common infection in end-stage liver disease patients.SBP is defined as an ascitic fluid infection with a polymorphonuclear leucocyte count ≥ 250/mm^3 without an evident intra-abdominal surgically treatable source.Several mechanisms contribute to SBP occurrence,including translocation of gut bacteria and their products,reduced intestinal motility provoking bacterial overgrowth,alteration of the gut's barrier function and local immune responses.Historically,Gram-negative enteric bacteria have been the main causative agents of SBP,thereby guiding the empirical therapeutic choice.However,over the last decade,a worryingly increasing prevalence of Gram-positive and multi-drug resistant(MDR) SBP has been seen.Recently,the microbiological spectrum of SBP seems to have changed in Europe due to a high prevalence of Gram-positive bacteria(48%-62%).The overall proportion of MDR bacteria is up to 22%-73% of cases.Consequently,empirical therapy based on thirdgeneration cephalosporins or amoxicillin/clavulanic acid,can no longer be considered the standard of care,as these drugs are associated with poor outcomes.Theaim of this review is to describe,with an epidemiological focus,the evidence behind this rise in Gram-positive and MDR SBP from 2000 to present,and illustrate potential targeted therapeutic strategies.An appropriate treatment protocol should include daptomycin plus ceftaroline and meropenem,with prompt stepdown to a narrower spectrum when cultures and sensitivity data are available in order to reduce both cost and potential antibiotic resistance development.

MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer

Chemotherapy resistance plays a pivotal role in the prognosis and therapeutic failure of patients with colorectal cancer(CRC).Cisplatin(DDP)-resistant cells exhibit an inherent ability to evade the toxic chemotherapeutic drug effects which are characterized by the activation of slow-cycle programs and DNA repair.Among the elements that lead to DDP resistance,O^(6)-methylguanine(O^(6)-MG)-DNA-methyltransferase(MGMT),a DNA-repair enzyme,performs a quintessential role.In this study,we clarify the significant involvement of MGMT in conferring DDP resistance in CRC,elucidating the underlying mechanism of the regulatory actions of MGMT.A notable upregulation of MGMT in DDP-resistant cancer cells was found in our study,and MGMT repression amplifies the sensitivity of these cells to DDP treatment in vitro and in vivo.Conversely,in cancer cells,MGMT overexpression abolishes their sensitivity to DDP treatment.Mechanistically,the interaction between MGMT and cyclin dependent kinase 1(CDK1)inducing slow-cycling cells is attainted via the promotion of ubiquitination degradation of CDK1.Meanwhile,to achieve nonhomologous end joining,MGMT interacts with XRCC6 to resist chemotherapy drugs.Our transcriptome data from samples of 88 patients with CRC suggest that MGMT expression is co-related with the Wnt signaling pathway activation,and several Wnt inhibitors can repress drug-resistant cells.In summary,our results point out that MGMT is a potential therapeutic target and predictive marker of chemoresistance in CRC.

A Loop Thermosyphon Type Cooling System for High Heat Flux

With rapid development of the semiconductor technology, more efficient cooling systems for electronic devices are needed. In this situation, in the present study, a loop thermosyphon type cooling system, which is composed mainly of a heating block, an evaporator and an air-cooled condenser, is investigated experimentally in order to evaluate the cooling performance. At first, it is examined that the optimum volume filling rate of this cooling system is approximately 40%. Next, four kinds of working fluids, R1234ze(E), R1234ze(Z), R134a and ethanol, are tested using a blasted heat transfer surface of the evaporator. In cases of R1234ze(E), R1234ze(Z), R134a and ethanol, the effective heat flux, at which the heating block surface temperature reaches 70°C, is 116 W/cm2, 106 W/cm2, 104 W/cm2 and 60 W/cm2, respectively. This result indicates that R1234ze(E) is the most suitable for the present cooling system. The minimum boiling thermal resistance of R1234ze(E) is 0.05 (cm2·K)/W around the effective heat flux of 100 W/cm2. Finally, four kinds of heat transfer surfaces of the evaporator, smooth, blasted, copper-plated and finned surfaces, are tested using R1234ze(E) as working fluid. The boiling thermal resistance of the blasted surface is the smallest among tested heat transfer surfaces up to 116 W/cm2 in effective heat flux. However, it increases drastically due to the appearance of dry-patch if the effective heat flux exceeds 116 W/cm2. On the other hand, in cases of copper-plated and finned surfaces, the dry-patch does not appear up to 150 W/cm2 in effective heat flux, and the boiling thermal resistances of those surfaces keep 0.1 (cm2·K)/W.

Parasitic Effects on the Performance of DC-DC SEPIC in Photovoltaic Maximum Power Point Tracking Applications

This paper presents an analysis of the effect of parasitic resistances on the performance of DC-DC Single Ended Pri- mary Inductor Converter (SEPIC) in photovoltaic maximum power point tracking (MPPT) applications. The energy storage elements incorporated in the SEPIC converter possess parasitic resistances. Although ideal components significantly simplifies model development, but neglecting the parasitic effects in models may sometimes lead to failure in predicting first scale stability and actual performance. Therefore, the effects of parasitics have been taken into consideration for improving the model accuracy, stability, robustness and dynamic performance analysis of the converter. Detail mathematical model of SEPIC converter including inductive parasitic has been developed. The performance of the converter in tracking MPP at different irradiance levels has been analyzed for variation in parasitic resistance. The converter efficiency has been found above 83% for insolation level of 600 W/m2 when the parasitic resistance in the energy storage element has been ignored. However, as the parasitic resistance of both of the inductor has increased to 1 ohm, a fraction of the power managed by the converter has dissipated;as a result the efficiency of the converter has reduced to 78% for the same insolation profile. Although the increasing value of the parasitic has assisted the converter to converge quickly to reach the maximum power point. Furthermore it has also been observed that the peak to peak load current ripple is reduced. The obtained simulation results have validated the competent of the MPPT converter model.

基于R-loop靶向编辑技术的R-loop功能位点高通量筛选系统

【目的】开发哺乳动物细胞R-loop靶向编辑技术,探究其在肿瘤细胞耐药性研究中的应用。【方法】构建无酶切活性的Cas9与具有R-loop水解活性的RNase H1融合蛋白dCas9-RNaseH1表达载体,用于实现对R-loop的靶向编辑。在HeLa细胞中稳定表达该融合蛋白,建立R-loop靶向编辑细胞模型。转染覆盖全基因组转录起始区域的sgRNA文库,构建R-loop筛选细胞库,筛选影响紫杉醇和顺铂耐药性的R-loop功能位点。【结果】筛选获得744个影响HeLa细胞耐药性的R-loop功能位点,覆盖了细胞周期、凋亡、信号传导等关键生物通路。其中,26个位点使HeLa细胞对这两种药物产生耐药性,8个位点使其对这两种药物敏感,提示可能存在共享的生物通路。功能验证显示,部分R-loop功能位点通过调节相关基因(如ZBTB20、SPON2、ACTRT1等)的表达来影响HeLa细胞对抗肿瘤药物的敏感性。【结论】成功开发出适用于哺乳动物细胞的R-loop靶向编辑系统,并建立高通量筛选平台。

A study on the integration of adeno-associated virus inverted terminal repeat fold structure in eukaryotic chromosome in vitro

Adeno-associated virus(AAV) is a single-stranded linear defective parvovirus. Using the model proposed by Cavalier-Smith for replication of ss-DNA virus. the linear fragment of inverted terminal repeats(ITRs) from PAAV/neo was isolated, denatured and refolded over. The daughter DNA was synthesized by using 3'-OH terminus as a primer with the new strand covalently linked to the parental strand. After the palindromic structure with neomycin resistance gene controlled by SV40 early promoter into eukaryotic cells. Southern blot showed that the molecule of hairpin structure could facilitate the copies into mammalian cells in vitro. The experiment supports the Cavalier-Smith's replication model of ss-DNA virus and provides another new system for DNA transduction.

Isolation and Characterization of NBS-LRR Class Resistance Homologous Gene from Wheat

One resistance gene analog fragment named RGA-CIN14 was isolated from TcLr19 wheat,which contains kinase-2,kinase-3a,and the GLPL motif of the NBS-spanning region,using degenerated primers according to the nucleotide binding site （NBS） conserved domain.Based on the RGA-CIN14,a full-length cDNA,CIN14,which was 2 987 bp encoding 880 amino acids,was obtained by using the method of the rapid amplification cDNA ends （RACE）.Bioinformatics analysis showed that the deduced amino acids of CIN14 protein consisted of a NB-ARC conserved domain and many leucine-rich repeats （LRR） domains.The phylogenetic tree analysis indicated a considerable identity of the protein encoded by CIN14 with that of wheat leaf rust resistance gene Lr1,but a lower similarity with Lr21.The expression profile of the CIN14 gene detected by semi-quantitative RT-PCR showed that the CIN14 gene was not induced by Puccinia triticina and it was a constitutive gene with low abundance in the wheat leaf tissue.The resistance homology sequence was successfully obtained,which provides the shortcut for cloning of the resistance gene in TcLr19 wheat.

Calculation of all-time apparent resistivity of large loop transient electromagnetic method with very fast simulated annealing

In large loop transient electromagnetic method(TEM),the late time apparent resistivity formula cannot truly reflect the geoelectric model,thus it needs to define the all-time apparent resistivity with the position information of measuring point.Utilizing very fast simulated annealing(VFSA) to fit the theoretical electromagnetic force(EMF) and measured EMF could obtain the all-time apparent resistivity of the measuring points in rectangular transmitting loop.The selective cope of initial model of VFSA could be confirmed by taking the late time apparent resistivity of transient electromagnetic method as the prior information.For verifying the correctness,the all-time apparent resistivities of the geoelectric models were calculated by VFSA and dichotomy,respectively.The results indicate that the relative differences of apparent resistivities calculated by these two methods are within 3%.The change of measuring point position has little influence on the tracing pattern of all-time apparent resistivity.The first branch of the curve of all-time apparent resistivity is close to the resistivity of the first layer medium and the last branch is close to the resistivity of the last layer medium,which proves the correctness of the arithmetics proposed.

Modeling of Continuous Cross-flow Microfiltration Process in an Airlift External-loop Slurry Reactor

New modified combination mathematical models including the pores blocking models and the cake layer models were developed to describe the continuous cross-flow microfiltration in an airlift external loop slurry reactor. The pores blocking models were created based on the standard blocking law and the intermediate blocking law, and then the cake layer models were developed based on the hydrodynamic theory in which the calculation method of porosity of cake layer was newly corrected. The Air-Water-FCC equilibrium catalysts cold model experiment was used to verify the relevant models.Results showed that the calculated values fitted well with experimental data with a relative error of less than 10%.

Development and mapping of SSR markers linked to resistance-gene homologue clusters in common bean

Common bean is an important but often a disease-susceptible legume crop of temperate,subtropical and tropical regions worldwide. The crop is affected by bacterial, fungal and viral pathogens. The strategy of resistance-gene homologue(RGH) cloning has proven to be an efficient tool for identifying markers and R(resistance) genes associated with resistances to diseases. Microsatellite or SSR markers can be identified by physical association with RGH clones on large-insert DNA clones such as bacterial artificial chromosomes(BACs). Our objectives in this work were to identify RGH-SSR in a BAC library from the Andean genotype G19833 and to test and map any polymorphic markers to identify associations with known positions of disease resistance genes. We developed a set of specific probes designed for clades of common bean RGH genes and then identified positive BAC clones and developed microsatellites from BACs having SSR loci in their end sequences. A total of 629 new RGH-SSRs were identified and named BMr(bean microsatellite RGH-associated markers). A subset of these markers was screened for detecting polymorphism in the genetic mapping population DOR364 × G19833. A genetic map was constructed with a total of 264 markers,among which were 80 RGH loci anchored to single-copy RFLP and SSR markers. Clusters of RGH-SSRs were observed on most of the linkage groups of common bean and in positions associated with R-genes and QTL. The use of these new markers to select for disease resistance is discussed.

End points of clinical trials in metastatic castration-resistant prostate cancer:A systematic review

AIM： To review the defnition and performance of the commonly used end points in trials of systemic thera-pies in metastatic castration-resistant prostate cancer patients. METHODS： A literature search was undertaken on PubMed database to identify studies meeting estab-lished criteria, with the aim of selecting randomized clinical trials and study definition and performance of their end points. The end points were grouped into three categories： overall survival （OS）, time-to-event end points, and response end points. A special analysis was performed for secondary end points of the studies which documented a beneft in OS in the experimental arm. Finally, publishes analyses for surrogacy of the in-cluded end points were also reported. RESULTS： OS, time-to-event and response end points in 31 selected trials were analyzed. OS was the pri-mary end point in 14 trials, and the secondary end point in 17. A time-to-event end point was the primaryend point in 8 studies, and the secondary end point in 22; the most reported time-to-event end points were composite end points, and the events changed among trials. A response end point was the primary end point in 9 studies, in 3 it was prostate-specifc antigen （PSA）-related, in 3 pain-related and in 3 mixed. A response end point was the secondary end point in 19 studies： PSA response and radiologic response were the most frequently used secondary end points in 19 and 11 tri-als, respectively, while pain response was used in 5 studies.CONCLUSION： A homogeneous defnition of progres-sion in future trials is mandatory. Among response end points, pain-response and PSA-response appear to be the most reliable.

Difficult Conversations and Painful Decisions: When Should Patients with Progressive Cancer Stop Chemotherapy?

Introduction: The decision to stop anti-cancer treatment is fraught with many challenges for the oncologist, the patient, and their caregivers. This review examines the special considerations surrounding the decision to cease chemotherapy in terminally ill cancer patient. Methods: A comprehensive literature search was conducted to find relevant publications on chemotherapy cessation. A total of 2700 records were retrieved and 141 were identified as eligible for inclusion in this review. Results: Palliative chemotherapy does not achieve the goal of tumor-related symptom reduction for patients who have experienced progressive disease with more than two prior lines of chemotherapy. ECOG performance status is a crucial predictor of response to therapy and chemotherapy-related complications. Challenges to stopping chemotherapy at the end of life are multifactorial and are both patient and physician-driven. Racial, ethnic, and income-based disparities are seen in the timing and quality of end-of-life conversations offered by physicians to their patients. Conclusions: The decision to cease chemotherapy is one that should be approached with careful consideration and accurate information. Clear communication, compassion and empathy are important components to the therapeutic relationship. Early involvement of palliative care and clear conversations about prognosis and the expected utility of further chemotherapy is essential to conduct the best possible care for cancer patients at the end of life.

基于端部绕组模块的定子励磁无刷电机冷却方法

为了提升定子励磁无刷电机的冷却能力,提出了一种基于端部绕组模块的定子励磁无刷电机冷却方法.在电机端部绕组和定子铁芯叠片端部的间隙中插入端部绕组冷却(EWC)模块,以减小定子绕组热点区域和冷却介质之间的间隙,从而提高端部绕组的冷却能力.对非重叠式集中绕组长度计算模型进行改进,评估采用EWC模块前后电机定子电枢绕组长度的变化,分析EWC模块的电导率对电机定子损耗的影响.采用计算流体动力学(CFD)模型,对比EWC模块在不同铜耗和励磁源损耗密度下的冷却性能.结果表明,采用EWC模块可以使端部绕组的稳态温升降低约50%,热态时的绕组电阻降低7.51%,从而改善了定子的冷却条件.

左室压力-应变环评估终末期肾病透析患者左室收缩功能的临床价值

目的探讨左室压力-应变环(LV-PSL)评估终末期肾病血液透析和腹膜透析患者左室收缩功能的临床应用价值。方法选取于我院就诊的79例慢性肾脏病5期患者,其中血液透析组29例、腹膜透析组27例及未透析组23例,均行常规超声心动图检查并获取相关参数,应用自动心肌功能成像分析软件获取左室整体纵向应变(GLS)及心肌做功指数(MWI),包括整体做功指数(GWI)、整体有效功(GCW)、整体无效功(GWW)、整体做功效率(GWE),比较各组上述参数的差异。分析MWI与常规超声心动图参数、GLS、血压、透析时间、透析方式的相关性。结果各组常规超声心动图参数和GLS比较差异均无统计学意义。血液透析组GWI、GCW均低于腹膜透析组和未透析组,GWW高于腹膜透析组和未透析组,GWE低于腹膜透析组,差异均有统计学意义(均P<0.05)。相关性分析显示,GWI、GCW与GLS、左室射血分数(LVEF)、收缩压、舒张压均呈正相关,与室间隔厚度(IVS)、透析时间、透析方式均呈负相关(均P<0.05);GWW与透析方式呈正相关,与GLS呈负相关(均P<0.05);GWE与GLS、LVEF均呈正相关,与IVS、左室后壁厚度、透析方式均呈负相关(均P<0.05)。结论血液透析对终末期肾病患者左室收缩功能的影响较腹膜透析更大,LV-PSL能准确评估该类患者左室收缩功能,具有较好的临床应用价值。