The development of the socialist market economy demands the furtherintensification of the reform of the management operating system (MOS)of the state-owned assets, the strengthening of the efficiency in the man-agemen...The development of the socialist market economy demands the furtherintensification of the reform of the management operating system (MOS)of the state-owned assets, the strengthening of the efficiency in the man-agement of state-owned assets,and the improvement of the operating bene-展开更多
On Sept.27, 2005, China Certification and Accreditation Association was set up, which was a sign of standardization and legalization for China certification and accreditation. Furthermore, its main task includes suppo...On Sept.27, 2005, China Certification and Accreditation Association was set up, which was a sign of standardization and legalization for China certification and accreditation. Furthermore, its main task includes supporting fairness, gaining credit, promoting development and building a harmonious society.展开更多
With the adoption of the policy of reform and opening to the outside world, more and more foreign enterprises (including overseas Chinese, Hongkong and Macao ones) wish to establish permanent representative offices in...With the adoption of the policy of reform and opening to the outside world, more and more foreign enterprises (including overseas Chinese, Hongkong and Macao ones) wish to establish permanent representative offices in China. In order to help foreign businessmen understand the rules and regulations and the procedures relating to the establishment of展开更多
Objective To establish stable prostate cancer bone metastasis cell line overexpressing microRAN-145 (miR-145)for the study of the mechanism of miR-145 in bone metastasis.Methods pMSCV-miR-145 plasmids and retroviruses...Objective To establish stable prostate cancer bone metastasis cell line overexpressing microRAN-145 (miR-145)for the study of the mechanism of miR-145 in bone metastasis.Methods pMSCV-miR-145 plasmids and retroviruses of pMSCV-vector展开更多
On November 30, 2007, the China Association for Standardization (CAS) held a press conference at Beijing Diaoyutai State Guest House. Leaders from the China Household Electric Appliance Research Institute, the Chi... On November 30, 2007, the China Association for Standardization (CAS) held a press conference at Beijing Diaoyutai State Guest House. Leaders from the China Household Electric Appliance Research Institute, the China Household Electric Appliance Association, and the China Consumers' Association attended and made speeches.……展开更多
Objective To establish a SOI model of human renal carcinoma and a high metastatic cell subline. Methods A human renal cell line RCC-9863 has been established by inoculating a human renal tumor tissue into nude mice s....Objective To establish a SOI model of human renal carcinoma and a high metastatic cell subline. Methods A human renal cell line RCC-9863 has been established by inoculating a human renal tumor tissue into nude mice s. c.. When RCC-9863 passaged for 20 times, the tissue from the same xemotransplant tumor were used to construct SOI model. Cultured the metastatic tissue in vitro, the tumor cell suspension was then injected orthotopically, The metastatic tissue obtained underwent the same procedure again. At last, the metastatic tumor was cultured in vitro and cloned. Results 15 days later, a tumor mass sized 1. 7 cm × 0. 6 cm in the nude mouse’s renal parenchyma was grown which lobulated, rude, and with multiply blood vessels and 55 days later later the mouse became moribund and metastases in the lungs were formed. The transplanted renal tumor in the SOI model grew fast and invasively and metastasized to lungs, lymphatic node and liver. A subline, MRCC, with metastatic ability to the lung was selected.展开更多
To investigate exogenous PTEN gene transfected human breast cancer cell line MDA-MD-468.Methods Using the lipofectamine 2000 transfection technique,wild type PTEN gene was transducted into an in vitro cultured highly ...To investigate exogenous PTEN gene transfected human breast cancer cell line MDA-MD-468.Methods Using the lipofectamine 2000 transfection technique,wild type PTEN gene was transducted into an in vitro cultured highly metastatic breast cancer cell line MDA-MD-468.After transfection,the cells were selected by G418.The resistant clones were chosen and expanded in DMEM culture medium.RT-PCR,immunohistochemical method and western blot were used to determine the expression of target genes.Results An anti-G418 cell clone was established and expanded in culture.The transfected PTEN gene MDA-MD-468 cells showed expression of PTEN mRNA and PTEN protein.Conclusion Human breast cancer cell line MDA-MB-468 established in this study expresses consistently exogenous PTEN genes.4 refs,6 figs.展开更多
It is known to all that traditional Chinese medicine and pharmacy, as the summary of Chinese people fighting against various kinds of disease for thousands of years, has a summate theoretical system possessing the ess...It is known to all that traditional Chinese medicine and pharmacy, as the summary of Chinese people fighting against various kinds of disease for thousands of years, has a summate theoretical system possessing the essence of Chinese classical philosophy, intensively reflects deep thinking and pondering of Chinese culture on the human being himself and dialectical relation between human and culture, making indelible contribution to the offspring reproduction and prosperity.展开更多
For the purpose of promoting the integration of traditional Chinese medicine and Western medicine on teaching, clinical and fundamental study, a tablet unveiled ceremony and academic congress was held in Beijing on Ap...For the purpose of promoting the integration of traditional Chinese medicine and Western medicine on teaching, clinical and fundamental study, a tablet unveiled ceremony and academic congress was held in Beijing on April 12, 2008.展开更多
For carrying out the Industrial Policy of the Motor Industry, competent authorities concerned are working intensely in the transition from mandatory standards to technical regulations, which includes the formulation o...For carrying out the Industrial Policy of the Motor Industry, competent authorities concerned are working intensely in the transition from mandatory standards to technical regulations, which includes the formulation of auto products (including motorcycles) according to the technical regulations, any products without type approval shall not be permitred to sell, import and use. To expedite the展开更多
文摘The development of the socialist market economy demands the furtherintensification of the reform of the management operating system (MOS)of the state-owned assets, the strengthening of the efficiency in the man-agement of state-owned assets,and the improvement of the operating bene-
文摘On Sept.27, 2005, China Certification and Accreditation Association was set up, which was a sign of standardization and legalization for China certification and accreditation. Furthermore, its main task includes supporting fairness, gaining credit, promoting development and building a harmonious society.
文摘With the adoption of the policy of reform and opening to the outside world, more and more foreign enterprises (including overseas Chinese, Hongkong and Macao ones) wish to establish permanent representative offices in China. In order to help foreign businessmen understand the rules and regulations and the procedures relating to the establishment of
文摘Objective To establish stable prostate cancer bone metastasis cell line overexpressing microRAN-145 (miR-145)for the study of the mechanism of miR-145 in bone metastasis.Methods pMSCV-miR-145 plasmids and retroviruses of pMSCV-vector
文摘 On November 30, 2007, the China Association for Standardization (CAS) held a press conference at Beijing Diaoyutai State Guest House. Leaders from the China Household Electric Appliance Research Institute, the China Household Electric Appliance Association, and the China Consumers' Association attended and made speeches.……
文摘Objective To establish a SOI model of human renal carcinoma and a high metastatic cell subline. Methods A human renal cell line RCC-9863 has been established by inoculating a human renal tumor tissue into nude mice s. c.. When RCC-9863 passaged for 20 times, the tissue from the same xemotransplant tumor were used to construct SOI model. Cultured the metastatic tissue in vitro, the tumor cell suspension was then injected orthotopically, The metastatic tissue obtained underwent the same procedure again. At last, the metastatic tumor was cultured in vitro and cloned. Results 15 days later, a tumor mass sized 1. 7 cm × 0. 6 cm in the nude mouse’s renal parenchyma was grown which lobulated, rude, and with multiply blood vessels and 55 days later later the mouse became moribund and metastases in the lungs were formed. The transplanted renal tumor in the SOI model grew fast and invasively and metastasized to lungs, lymphatic node and liver. A subline, MRCC, with metastatic ability to the lung was selected.
文摘To investigate exogenous PTEN gene transfected human breast cancer cell line MDA-MD-468.Methods Using the lipofectamine 2000 transfection technique,wild type PTEN gene was transducted into an in vitro cultured highly metastatic breast cancer cell line MDA-MD-468.After transfection,the cells were selected by G418.The resistant clones were chosen and expanded in DMEM culture medium.RT-PCR,immunohistochemical method and western blot were used to determine the expression of target genes.Results An anti-G418 cell clone was established and expanded in culture.The transfected PTEN gene MDA-MD-468 cells showed expression of PTEN mRNA and PTEN protein.Conclusion Human breast cancer cell line MDA-MB-468 established in this study expresses consistently exogenous PTEN genes.4 refs,6 figs.
文摘It is known to all that traditional Chinese medicine and pharmacy, as the summary of Chinese people fighting against various kinds of disease for thousands of years, has a summate theoretical system possessing the essence of Chinese classical philosophy, intensively reflects deep thinking and pondering of Chinese culture on the human being himself and dialectical relation between human and culture, making indelible contribution to the offspring reproduction and prosperity.
文摘For the purpose of promoting the integration of traditional Chinese medicine and Western medicine on teaching, clinical and fundamental study, a tablet unveiled ceremony and academic congress was held in Beijing on April 12, 2008.
文摘For carrying out the Industrial Policy of the Motor Industry, competent authorities concerned are working intensely in the transition from mandatory standards to technical regulations, which includes the formulation of auto products (including motorcycles) according to the technical regulations, any products without type approval shall not be permitred to sell, import and use. To expedite the
