RNA-seq and single-cell genomic research emerge as an important research area in the recent years due to its ability to examine genetic information of any number of single cells in all living organisms.The knowledge g...RNA-seq and single-cell genomic research emerge as an important research area in the recent years due to its ability to examine genetic information of any number of single cells in all living organisms.The knowledge gained from RNA-seq and single-cell genomic research will have a great impact in many aspects of plant biology.In this review,we summary and discuss the biological significance of RNA-seq and single-cell genomic research in plants including the single-cell DNA-sequencing,RNA-seq and single-cell RNA sequencing in woody plants,methods of RNA-seq and single-cell RNA-sequencing,single-cell RNA-sequencing for studying plant development,and single-cell RNA-sequencing for elucidating cell type composition.We will focus on RNA-seq and single-cell RNA sequencing in woody plants,understanding of plant development through single-cell RNAsequencing,and elucidation of cell type composition via single-cell RNA-sequencing.Information presented in this review will be helpful to increase our understanding of plant genomic research in a way with the power of plant single-cell RNA-sequencing analysis.展开更多
为筛选出提取梨叶片总RNA的最佳方法,以梨极矮化突变体和苹果梨为材料,采用柱式植物RNAout试剂盒、SDS法和改良CTAB法提取其叶片总RNA并比较提取效果。结果表明,改良CTAB法能有效抑制酚类物质和多糖对总RNA提取的影响,获得纯度高、完整...为筛选出提取梨叶片总RNA的最佳方法,以梨极矮化突变体和苹果梨为材料,采用柱式植物RNAout试剂盒、SDS法和改良CTAB法提取其叶片总RNA并比较提取效果。结果表明,改良CTAB法能有效抑制酚类物质和多糖对总RNA提取的影响,获得纯度高、完整性好的总RNA,其28 S rRNA条带亮度高于18 S rRNA,所提取的RNA适合下一步的研究使用。展开更多
为了提高植物不同组织提取质量和效率,降低成本,在热硼酸法的基础上探索出一种适于植物不同组织总RNA提取方法。利用该方法从植物根、茎、叶、不同成熟期果实、不同成熟期种子和不同成熟期果柄中提取的总RNA经紫外分光光度计和琼脂糖凝...为了提高植物不同组织提取质量和效率,降低成本,在热硼酸法的基础上探索出一种适于植物不同组织总RNA提取方法。利用该方法从植物根、茎、叶、不同成熟期果实、不同成熟期种子和不同成熟期果柄中提取的总RNA经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性。所得RNA的A260/A230大于2.3,A260/A280为1.8～2.0,并且RNA产量高,可以满足RT-PCR和Real time RT-PCR的实验需要。该方法能够满足一般分子生物学下游操作的要求,是一种理想的植物不同组织总RNA提取方法。展开更多
文摘RNA-seq and single-cell genomic research emerge as an important research area in the recent years due to its ability to examine genetic information of any number of single cells in all living organisms.The knowledge gained from RNA-seq and single-cell genomic research will have a great impact in many aspects of plant biology.In this review,we summary and discuss the biological significance of RNA-seq and single-cell genomic research in plants including the single-cell DNA-sequencing,RNA-seq and single-cell RNA sequencing in woody plants,methods of RNA-seq and single-cell RNA-sequencing,single-cell RNA-sequencing for studying plant development,and single-cell RNA-sequencing for elucidating cell type composition.We will focus on RNA-seq and single-cell RNA sequencing in woody plants,understanding of plant development through single-cell RNAsequencing,and elucidation of cell type composition via single-cell RNA-sequencing.Information presented in this review will be helpful to increase our understanding of plant genomic research in a way with the power of plant single-cell RNA-sequencing analysis.
文摘为筛选出提取梨叶片总RNA的最佳方法,以梨极矮化突变体和苹果梨为材料,采用柱式植物RNAout试剂盒、SDS法和改良CTAB法提取其叶片总RNA并比较提取效果。结果表明,改良CTAB法能有效抑制酚类物质和多糖对总RNA提取的影响,获得纯度高、完整性好的总RNA,其28 S rRNA条带亮度高于18 S rRNA,所提取的RNA适合下一步的研究使用。
文摘为了提高植物不同组织提取质量和效率,降低成本,在热硼酸法的基础上探索出一种适于植物不同组织总RNA提取方法。利用该方法从植物根、茎、叶、不同成熟期果实、不同成熟期种子和不同成熟期果柄中提取的总RNA经紫外分光光度计和琼脂糖凝胶电泳分析证明具有较高的纯度和完整性。所得RNA的A260/A230大于2.3,A260/A280为1.8～2.0,并且RNA产量高,可以满足RT-PCR和Real time RT-PCR的实验需要。该方法能够满足一般分子生物学下游操作的要求,是一种理想的植物不同组织总RNA提取方法。
