The effects of two different nitrogen fertilizers (urea and NH4Cl) with monocalcium phosphate (MCP) on the movement and transformation of fertilizer P in soil microsites along with soil pH changes at different distanc...The effects of two different nitrogen fertilizers (urea and NH4Cl) with monocalcium phosphate (MCP) on the movement and transformation of fertilizer P in soil microsites along with soil pH changes at different distances from the fertilizer application site were studied in an incubation experiment. A highly acidic red soil (Ultisol, pH 4.57) from south China with MCP fertilizer alone or in combination with NH4Cl or urea was added to the surface of soil cylinders and packed in wax blocks. After 7 and 28 days, the extraction and analysis of each 2 mm layer from the interface of the soil and fertilizer showed that added NH4Cl or urea did not change the movement distance of fertilizer P. However, P transformation was significantly affected (P < 0.05). After 7 days, at 0-8 mm distance from the fertilizer site the addition of urea significantly decreased the water-extractable P concentration; however, after 28 days the effect of N addition had disappeared. Also, at limited distances close to the fertilizer site NH4Cl application with MCP significantly increased acid-extractable P and available P, while with the addition of urea they significantly decreased. Compared with application of MCP alone, addition of urea significantly increased soil pH in fertilizer microsites, whereas the addition of NH4Cl significantly decreased soil pH.展开更多
Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of ea...Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV(SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast twohybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus.展开更多
Tectonic movements and climate changes are two main controllers on the development of landform. In order to reconstruct the history of the evolution of the landform in the Fenhe drainage basin d...Tectonic movements and climate changes are two main controllers on the development of landform. In order to reconstruct the history of the evolution of the landform in the Fenhe drainage basin during middle-late Quaternary comprehensively, this paper has provided a variety of geomorphological and geologic evidences to discuss how tectonic movements and climate changes worked together to influence the landform processes. According to the features of the lacustrine and alluvial terraces in this drainage basin, it is deduced that it was the three tectonic uplifts that resulted in the three great lake-regressions with an extent of about 40-60 m and the formation of the three lacustrine terraces. The times when the tectonic uplifts took place are 0.76 MaBP, 0.55 MaBP and 0.13 MaBP respectively, synchronous with the formation of paleosol units S 8 , S 5 and S 1 respectively. During the intervals between two tectonic uplifts when tectonic movement was very weak, climate changes played a major role in the evolution of the paleolakes and caused frequent fluctuations of lake levels. The changes of the features of lacustrine sediment in the grabens show the extent of such fluctuations of lake level is about 2-3 m.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 40071051) and the National Key BasicResearch Support Foundation of China (No. G1999011802).
文摘The effects of two different nitrogen fertilizers (urea and NH4Cl) with monocalcium phosphate (MCP) on the movement and transformation of fertilizer P in soil microsites along with soil pH changes at different distances from the fertilizer application site were studied in an incubation experiment. A highly acidic red soil (Ultisol, pH 4.57) from south China with MCP fertilizer alone or in combination with NH4Cl or urea was added to the surface of soil cylinders and packed in wax blocks. After 7 and 28 days, the extraction and analysis of each 2 mm layer from the interface of the soil and fertilizer showed that added NH4Cl or urea did not change the movement distance of fertilizer P. However, P transformation was significantly affected (P < 0.05). After 7 days, at 0-8 mm distance from the fertilizer site the addition of urea significantly decreased the water-extractable P concentration; however, after 28 days the effect of N addition had disappeared. Also, at limited distances close to the fertilizer site NH4Cl application with MCP significantly increased acid-extractable P and available P, while with the addition of urea they significantly decreased. Compared with application of MCP alone, addition of urea significantly increased soil pH in fertilizer microsites, whereas the addition of NH4Cl significantly decreased soil pH.
基金supported by the grants from the National Natural Science Foundation of China(32072386 and 31801700)the Key Research and Development Project of Anhui Province,China(202004a06020013)the Postdoctoral Science Fund of Anhui Province,China(2019B360)。
文摘Although the complete nucleotide sequence of strawberry vein banding virus(SVBV) has been determined and bioinformatic analysis has revealed that the SVBV genome could encode seven proteins, the precise function of each protein is unclear. This study provided evidence that the P1 protein of SVBV(SVBV-P1) possesses the following features. Bioinformatic and subcellular localization analyses showed that SVBV-P1 is localized in the cytoplasm and cell walls of epidermal cells in Nicotiana benthamiana, and it forms inclusion bodies associated with microtubules and the endoplasmic reticulum. Dilution experiments demonstrated that SVBV-P1 could move from the original agro-infiltrated cells to adjacent cells in N. benthamiana leaves. Further trans-complementation experiments demonstrated that SVBV-P1 could facilitate the intercellular movement of a movement-deficient potato virus X mutant in N. benthamiana leaves. Finally, yeast twohybrid and bimolecular fluorescence complementation assays revealed that SVBV-P1 could interact with the SVBV coat protein, which is a major component of Caulimovirus virions. Results of the electrophoretic mobility shift assay indicated that SVBV-P1 lacks DNA-binding capability. In summary, the results suggest that SVBV-P1 is probably a movement protein of SVBV, providing new insights into the function of movement proteins of the Caulimovirus genus.
基金National Natural Science Foundation of China No.40071016
文摘Tectonic movements and climate changes are two main controllers on the development of landform. In order to reconstruct the history of the evolution of the landform in the Fenhe drainage basin during middle-late Quaternary comprehensively, this paper has provided a variety of geomorphological and geologic evidences to discuss how tectonic movements and climate changes worked together to influence the landform processes. According to the features of the lacustrine and alluvial terraces in this drainage basin, it is deduced that it was the three tectonic uplifts that resulted in the three great lake-regressions with an extent of about 40-60 m and the formation of the three lacustrine terraces. The times when the tectonic uplifts took place are 0.76 MaBP, 0.55 MaBP and 0.13 MaBP respectively, synchronous with the formation of paleosol units S 8 , S 5 and S 1 respectively. During the intervals between two tectonic uplifts when tectonic movement was very weak, climate changes played a major role in the evolution of the paleolakes and caused frequent fluctuations of lake levels. The changes of the features of lacustrine sediment in the grabens show the extent of such fluctuations of lake level is about 2-3 m.
