Culture teaching is an inseparable part of International Chinese Language Education,and it has great significance in international Chinese language teaching.Students can enhance their interest in Chinese language lear...Culture teaching is an inseparable part of International Chinese Language Education,and it has great significance in international Chinese language teaching.Students can enhance their interest in Chinese language learning and improve their learning effect through understanding the excellent traditional Chinese culture,at the same time,culture teaching can help to disseminate the Chinese culture and promote the development of a multicultural world.Taking the teaching of Chinese New Year culture as an example,we analyze the current teaching materials involving Chinese New Year culture with high popularity and acceptance,put forward effective teaching methods matching the feasibility of teaching Chinese New Year culture,and then present the specific teaching design of Chinese New Year culture,in order to provide references for the teaching of culture in International Chinese Language Education.展开更多
The classroom is an important place for“three guarantees”education.Classroom culture refers to a group culture with both teachers and students as the main body,the classroom space as the carrier,and spiritual connot...The classroom is an important place for“three guarantees”education.Classroom culture refers to a group culture with both teachers and students as the main body,the classroom space as the carrier,and spiritual connotation as the core.The creation of classroom culture with school characteristics,characteristics of the times,and Chinese characteristics can reflect the aesthetic perspective and value orientation of the school,which has far-reaching significance for the growth of students and their future development.This paper takes the construction of classroom culture in colleges and universities as the research subject,obtains real data,and conducts variable analysis through questionnaire survey and structured interview,with the aim of enriching the educational function of the classroom.It involves how the classroom culture pays attention to the current life,needs,and desires of college and university students,along with the problems and functions that should be improved in the traditional construction.This paper also discusses and reshapes the new paradigm of classroom culture construction in colleges and universities based on the combination of student growth,subject areas,school culture,and advanced technology.展开更多
AIM:To investigate the performance of the microcapillary culture method(MCM) in Helicobacter pylori(H.pylori) isolation and diagnosis.METHODS:Microcapillary culture(MC),classical culture(CC),rapid urease(CLO) test,and...AIM:To investigate the performance of the microcapillary culture method(MCM) in Helicobacter pylori(H.pylori) isolation and diagnosis.METHODS:Microcapillary culture(MC),classical culture(CC),rapid urease(CLO) test,and histopathologic examination(HE) were performed with biopsy samples.Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 ℃ without providing a microaerophilic environment.Additionally,three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium(Becton Dickinson,Helicobacter Agar,Modified) specific for the isolation of H.pylori and incubated at 37 ℃ in a microaerophilic atmosphere provided by Campy Gen(Becton Dickinson,Gas Pack).Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content.Results obtained by CC,CLO test,and HE were compared with those of MC.The diagnostic performances of the methods used in this study were evaluated for specificity,sensitivity,positive predictive value(PPV),negative predictive value(NPV),and CI.RESULTS:H.pylori was found positive by CLO test +HE and/or CC culture in 26 patient antrum and corpus biopsy samples.In 25(25/26) patient biopsy samples,H.pylori was isolated by MCM,whereas in only 14(14/26) patient biopsy samples,H.pylori was isolatedby CC.CLO test and HE were found positive in 17(17/26) patient biopsy samples.Comparing the results of the isolation of H.pylori by MCM,CC,CLO test,and HE,the sensitivity of the MCM was found as 96%,the specificity as 80%,the PPV as 83%,the NPV as 95%,and the 95%CI as 0.76(χ2 =31.51,P < 0.01) whereas the sensitivity of the CC was found as 54%(χ2 =19.15,P < 0.01),and the sensitivity of the CLO test and HE were found as 65%(χ2 =25.26,P < 0.01).CONCLUSION:This new microcapillary cultivation method for H.pylori has high diagnostic sensitivity compared with CC,HE,and CLO tests.展开更多
Developing digital culture industries is one of the important social planning strategies in China in the new era.China’s digital culture industries has continued to grow in recent years,but there are still a series o...Developing digital culture industries is one of the important social planning strategies in China in the new era.China’s digital culture industries has continued to grow in recent years,but there are still a series of problems such as imperfect industrial ecological chain structure,lack of development and innovation content,and small scale of ecological platform.At this stage,in order to build a sustainable ecological system for the development of digital culture industries in the new era,we should focus on the development of key areas related to digital culture industries,guide the development of industrial agglomeration,cultivate large-scale leading enterprises,strengthen the support of industry chain,build an innovative ecological model,and increase policy support.Only in this way can the digital culture industries be pushed into a benign ecological system as soon as possible and cultivated into a new momentum for the development of China’s culture industries in the new era.展开更多
With the increasing popularity of new media,new media marketing plays an increasingly important role in the marketing of tourism scenic spots,bringing a new marketing opportunity to tourism scenic spots.Based on the 4...With the increasing popularity of new media,new media marketing plays an increasingly important role in the marketing of tourism scenic spots,bringing a new marketing opportunity to tourism scenic spots.Based on the 4R theory in marketing theory,this paper takes Quzhou Jianglang Mountain as the research object and finds that the following problems exist in the new media marketing of Quzhou Jianglang Mountain:1.The new media users are not closely enough connected;2.the response to the new media market changes is not fast enough;3.the new media marketing relationship interaction is not timely enough;and 4.the return of new media marketing is not ideal.The new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot is proposed from four perspectives:association,reaction,relationship,and return.Finally,the research on the new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot based on the 4R theory is summarized.展开更多
Since the 18 th CPC National Congress,the Party Central Committee,with Comrade Xi Jinping as the core,has formed Xi Jinping Thought on Socialism with Chinese Characteristics( Xi's TSCC) for a New Era in the proces...Since the 18 th CPC National Congress,the Party Central Committee,with Comrade Xi Jinping as the core,has formed Xi Jinping Thought on Socialism with Chinese Characteristics( Xi's TSCC) for a New Era in the process of upholding and developing the great practice of Socialism with Chinese Characteristics( SCC). As an important part of Xi's TSCC for a new era, cultural thought takes the persistence of cultural confidence as its basic foundation,t he promotion of outstanding traditional culture as its important measure,the enhancement of cultural soft power as its strategic goal,t he cultivation &practice of core socialist values as its guide, and the improvement of clean government culture as its fundamental significance,t hus constituting a scientific system with complete structure, rich content and rigorous exposition. This important thought provides a powerful theoretical and practical guide to the work of building a socialist culture with Chinese characteristics in the new era.展开更多
Beautiful country of Yuezhao Community,Zhongshan District is taken as the case.By digging cultural characteristics and connotation of historic buildings and integrating historical culture resources,protection region o...Beautiful country of Yuezhao Community,Zhongshan District is taken as the case.By digging cultural characteristics and connotation of historic buildings and integrating historical culture resources,protection region of historic site is formed.By analyzing construction condition and environment of status quo and combining surrounding natural environment,greening and activity places are arranged in the area unsuitable for construction,and landscape node rich in local culture characteristics is created.In the planning,it should sufficiently respect layout of the existing buildings,making planning region become a new rural residential community in the new era that local culture atmosphere is rich;the planned buildings have the same skin textures with original buildings and harmoniously coexist with natural environment.展开更多
Nowadays,the communication of traditional culture is facing various challenges,including limitations of traditional culture itself,decrease of inheritors,poor construction of structure,and heterization of connotation,...Nowadays,the communication of traditional culture is facing various challenges,including limitations of traditional culture itself,decrease of inheritors,poor construction of structure,and heterization of connotation,as well as conflicts between Chinese and foreign cultures in the context of globalization.New media has provided more opportunities for the communication of outstanding Chinese traditional culture.This paper analyzed the role of new media in completing the structure and expanding the scope of communication of outstanding Chinese traditional culture,and proposed strategy for optimizing the communication route from the perspective of improving environment,changing content,and adjusting strategy.The contributions of new media to the communication of Chinese traditional culture should be highly appreciated.展开更多
Objective:To conduct a comparative analysis of the VP4 gene sequences of Indian wild type (06361,0613158,061060 and 0715880) and cell culture adapted(06361-CA,0613158-CA.061060- CA and 0715880-CA) G1P[8]rotavirus stra...Objective:To conduct a comparative analysis of the VP4 gene sequences of Indian wild type (06361,0613158,061060 and 0715880) and cell culture adapted(06361-CA,0613158-CA.061060- CA and 0715880-CA) G1P[8]rotavirus strains.Methods:Full-length VP4 genes of each of the four wild type G1P[8]rotavirus strains and their cell culture adapted counterparts displaying consistent cytopathic effect were subjected to RT-PCR amplification and nucleotide sequencing. Results:All four cell culture adapted G1P[8]rotavirus strains showed nucleotide and amino acid substitutions in the VP4 gene as compared to their wild type strains.The number of substitutions however,varied from 1-64 and 1-13 respectively.The substitutions were distributed in both VP5* and VP8* subunits of VP4 gene respectively of permeabilizalion and hemagglutinaling activity. The presence of unique amino acid substitutions was identified in two of the four wild type(V377G. S387N in 061060 and 1644L in 0715880) and all four cell culture adapted(A46V in 0613158-CA. T60R in 06361-CA,L237V.G389V and Q480H in 061060-CA and S615G and T625P in 0715880-CA) strains for the first time in the VP4 gene of P[8]specificity.Amino acid substitutions generated increase in the hydrophilicity in the cell culture adapted rotavirus strains as compared to their corresponding wild type strains.Conclusions:Amino acid substitutions detected in the VP4 genes of G1P[8]rotavirus strains from this study together with those from other studies highlight occurrence of only strain and/or host specific substitutions during cell culture adaptation. Further evaluation of such substitutions for their role in attenuation,immunogenicity and conformation is needed for the development of newer rolavirus vaccines.展开更多
In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studi...In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10-15 μmol·L-1 CuSO4 and 0.50-1.00 mg·L-1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol·L-1. When the concentration of CuSO4 was 15 μmol·L-1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol·L-1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg·L-1 and for Longjing 24 was 1.00 mg·L-1.展开更多
The Chongqing Tourism and Culture Carnival Gala was held on the afternoon of May 1 0 in the New Zealand National Museum. It was co-hosted by China Cultural Center in New Zealand, the Wellington City Council, Chongqing...The Chongqing Tourism and Culture Carnival Gala was held on the afternoon of May 1 0 in the New Zealand National Museum. It was co-hosted by China Cultural Center in New Zealand, the Wellington City Council, Chongqing Culture and Tourism Development Committee and Chongqing Cultural Exchange Center. It was part of the 2019 China Tourism and Culture Week and the China-New Zealand Year of Tourism for the cooperation and communication in culture and tourism.展开更多
On January 16,"Happy Chinese New Year" 2019 Launching Ceremony and Press Conference was held in Beijing."Happy Chinese New Year" 2019 has carried out more than 1500 activities in 133 countries and ...On January 16,"Happy Chinese New Year" 2019 Launching Ceremony and Press Conference was held in Beijing."Happy Chinese New Year" 2019 has carried out more than 1500 activities in 133 countries and regions in over 40 forms such as temple fairs, exhibitions, performances, lectures, forums and interaction in intangible cultural heritage programs. Taking the opportunity of "Happy Chinese New Year" 2019, Chinese Embassies and Consulates in foreign countries, overseas China culture centers and overseas Chinese tourism offices have explored the integration of culture and tourism and focused on promoting the integration of ideas, with market and local culture. This contributes to showing Chinese New Year culture and telling China's story, making Chinese New Year an international festival shared by the world.展开更多
文摘Culture teaching is an inseparable part of International Chinese Language Education,and it has great significance in international Chinese language teaching.Students can enhance their interest in Chinese language learning and improve their learning effect through understanding the excellent traditional Chinese culture,at the same time,culture teaching can help to disseminate the Chinese culture and promote the development of a multicultural world.Taking the teaching of Chinese New Year culture as an example,we analyze the current teaching materials involving Chinese New Year culture with high popularity and acceptance,put forward effective teaching methods matching the feasibility of teaching Chinese New Year culture,and then present the specific teaching design of Chinese New Year culture,in order to provide references for the teaching of culture in International Chinese Language Education.
文摘The classroom is an important place for“three guarantees”education.Classroom culture refers to a group culture with both teachers and students as the main body,the classroom space as the carrier,and spiritual connotation as the core.The creation of classroom culture with school characteristics,characteristics of the times,and Chinese characteristics can reflect the aesthetic perspective and value orientation of the school,which has far-reaching significance for the growth of students and their future development.This paper takes the construction of classroom culture in colleges and universities as the research subject,obtains real data,and conducts variable analysis through questionnaire survey and structured interview,with the aim of enriching the educational function of the classroom.It involves how the classroom culture pays attention to the current life,needs,and desires of college and university students,along with the problems and functions that should be improved in the traditional construction.This paper also discusses and reshapes the new paradigm of classroom culture construction in colleges and universities based on the combination of student growth,subject areas,school culture,and advanced technology.
基金Supported by Yildiz Technical University Scientific Research Projects Coordinatorship,No.2012-07-04-KAP09
文摘AIM:To investigate the performance of the microcapillary culture method(MCM) in Helicobacter pylori(H.pylori) isolation and diagnosis.METHODS:Microcapillary culture(MC),classical culture(CC),rapid urease(CLO) test,and histopathologic examination(HE) were performed with biopsy samples.Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 ℃ without providing a microaerophilic environment.Additionally,three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium(Becton Dickinson,Helicobacter Agar,Modified) specific for the isolation of H.pylori and incubated at 37 ℃ in a microaerophilic atmosphere provided by Campy Gen(Becton Dickinson,Gas Pack).Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content.Results obtained by CC,CLO test,and HE were compared with those of MC.The diagnostic performances of the methods used in this study were evaluated for specificity,sensitivity,positive predictive value(PPV),negative predictive value(NPV),and CI.RESULTS:H.pylori was found positive by CLO test +HE and/or CC culture in 26 patient antrum and corpus biopsy samples.In 25(25/26) patient biopsy samples,H.pylori was isolated by MCM,whereas in only 14(14/26) patient biopsy samples,H.pylori was isolatedby CC.CLO test and HE were found positive in 17(17/26) patient biopsy samples.Comparing the results of the isolation of H.pylori by MCM,CC,CLO test,and HE,the sensitivity of the MCM was found as 96%,the specificity as 80%,the PPV as 83%,the NPV as 95%,and the 95%CI as 0.76(χ2 =31.51,P < 0.01) whereas the sensitivity of the CC was found as 54%(χ2 =19.15,P < 0.01),and the sensitivity of the CLO test and HE were found as 65%(χ2 =25.26,P < 0.01).CONCLUSION:This new microcapillary cultivation method for H.pylori has high diagnostic sensitivity compared with CC,HE,and CLO tests.
文摘Developing digital culture industries is one of the important social planning strategies in China in the new era.China’s digital culture industries has continued to grow in recent years,but there are still a series of problems such as imperfect industrial ecological chain structure,lack of development and innovation content,and small scale of ecological platform.At this stage,in order to build a sustainable ecological system for the development of digital culture industries in the new era,we should focus on the development of key areas related to digital culture industries,guide the development of industrial agglomeration,cultivate large-scale leading enterprises,strengthen the support of industry chain,build an innovative ecological model,and increase policy support.Only in this way can the digital culture industries be pushed into a benign ecological system as soon as possible and cultivated into a new momentum for the development of China’s culture industries in the new era.
基金sponsored by the following projects:Multi-modal Discourse Analysis of the Quzhou Government Affairs New Media(Grant No.202111488039).
文摘With the increasing popularity of new media,new media marketing plays an increasingly important role in the marketing of tourism scenic spots,bringing a new marketing opportunity to tourism scenic spots.Based on the 4R theory in marketing theory,this paper takes Quzhou Jianglang Mountain as the research object and finds that the following problems exist in the new media marketing of Quzhou Jianglang Mountain:1.The new media users are not closely enough connected;2.the response to the new media market changes is not fast enough;3.the new media marketing relationship interaction is not timely enough;and 4.the return of new media marketing is not ideal.The new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot is proposed from four perspectives:association,reaction,relationship,and return.Finally,the research on the new media marketing strategy of Quzhou Jianglang Mountain Scenic Spot based on the 4R theory is summarized.
基金This paper is the achievement of the special topic of the National Social Science Foundation of the 19th National Congress of the CPC-“The study on the theoretical innovation of Xi Jinping Thought on Socialism with Chinese Characteristics for a New era”(18VSJ001)
文摘Since the 18 th CPC National Congress,the Party Central Committee,with Comrade Xi Jinping as the core,has formed Xi Jinping Thought on Socialism with Chinese Characteristics( Xi's TSCC) for a New Era in the process of upholding and developing the great practice of Socialism with Chinese Characteristics( SCC). As an important part of Xi's TSCC for a new era, cultural thought takes the persistence of cultural confidence as its basic foundation,t he promotion of outstanding traditional culture as its important measure,the enhancement of cultural soft power as its strategic goal,t he cultivation &practice of core socialist values as its guide, and the improvement of clean government culture as its fundamental significance,t hus constituting a scientific system with complete structure, rich content and rigorous exposition. This important thought provides a powerful theoretical and practical guide to the work of building a socialist culture with Chinese characteristics in the new era.
基金Sponsored by 2016 Provincial Public Welfare Technology Application Research Plan Project from Department of Science and Technology of Zhejiang Province(2016C33251)Fumin Plan Project,Ningbo Municipal Science and Technology Bureau(2015C50002)Project from Ministry of Housing and Urban Rural Development(2015-K7-008)
文摘Beautiful country of Yuezhao Community,Zhongshan District is taken as the case.By digging cultural characteristics and connotation of historic buildings and integrating historical culture resources,protection region of historic site is formed.By analyzing construction condition and environment of status quo and combining surrounding natural environment,greening and activity places are arranged in the area unsuitable for construction,and landscape node rich in local culture characteristics is created.In the planning,it should sufficiently respect layout of the existing buildings,making planning region become a new rural residential community in the new era that local culture atmosphere is rich;the planned buildings have the same skin textures with original buildings and harmoniously coexist with natural environment.
基金the research findings of the research project of Sichuan International Studies University(Serial number:Sisu2019016)Project Title The Impact of New Media Hotspots on Urban Tourism
文摘Nowadays,the communication of traditional culture is facing various challenges,including limitations of traditional culture itself,decrease of inheritors,poor construction of structure,and heterization of connotation,as well as conflicts between Chinese and foreign cultures in the context of globalization.New media has provided more opportunities for the communication of outstanding Chinese traditional culture.This paper analyzed the role of new media in completing the structure and expanding the scope of communication of outstanding Chinese traditional culture,and proposed strategy for optimizing the communication route from the perspective of improving environment,changing content,and adjusting strategy.The contributions of new media to the communication of Chinese traditional culture should be highly appreciated.
基金supported by National Institute of Virology(NIV,Indian Council of Medical Research, Govt.of India),Pune
文摘Objective:To conduct a comparative analysis of the VP4 gene sequences of Indian wild type (06361,0613158,061060 and 0715880) and cell culture adapted(06361-CA,0613158-CA.061060- CA and 0715880-CA) G1P[8]rotavirus strains.Methods:Full-length VP4 genes of each of the four wild type G1P[8]rotavirus strains and their cell culture adapted counterparts displaying consistent cytopathic effect were subjected to RT-PCR amplification and nucleotide sequencing. Results:All four cell culture adapted G1P[8]rotavirus strains showed nucleotide and amino acid substitutions in the VP4 gene as compared to their wild type strains.The number of substitutions however,varied from 1-64 and 1-13 respectively.The substitutions were distributed in both VP5* and VP8* subunits of VP4 gene respectively of permeabilizalion and hemagglutinaling activity. The presence of unique amino acid substitutions was identified in two of the four wild type(V377G. S387N in 061060 and 1644L in 0715880) and all four cell culture adapted(A46V in 0613158-CA. T60R in 06361-CA,L237V.G389V and Q480H in 061060-CA and S615G and T625P in 0715880-CA) strains for the first time in the VP4 gene of P[8]specificity.Amino acid substitutions generated increase in the hydrophilicity in the cell culture adapted rotavirus strains as compared to their corresponding wild type strains.Conclusions:Amino acid substitutions detected in the VP4 genes of G1P[8]rotavirus strains from this study together with those from other studies highlight occurrence of only strain and/or host specific substitutions during cell culture adaptation. Further evaluation of such substitutions for their role in attenuation,immunogenicity and conformation is needed for the development of newer rolavirus vaccines.
基金Supported by Program in 12th Five-year Plan Rural Areas National Science and Technology Plan(2013BAD20B04)Tackle Key in Science and Technology of Chinese Science and Technology Ministry(2011BAD35B02-01)Program in Sience and Technology of Chinese Science and Technology Ministry(2011BAD16B11)
文摘In order to establish the system of high frequency plant regeneration for japonica rice mature embryos, the effects of different concentrations of CuSO4 and uniconazole on in vitro culture of mature embryos were studied using three rice cultivars of Kongyu 131, Longjing 24, and Dongnong 425 as test materials. The results showed that callus induction and differentiation of japonica rice mature embryos were apparently improved on the medium with 10-15 μmol·L-1 CuSO4 and 0.50-1.00 mg·L-1 uniconazole. Induction and differentiation rates of different genotype rice mature embryos displayed different sensitivities to CuSO4 and uniconazole. For the callus induction frequency of three varieties, the optimal concentration of CuSO4 was 15.0 mol·L-1. When the concentration of CuSO4 was 15 μmol·L-1, the plantlet differentiation rates of Kongyu 131 and Dongnong 425 got to the highest, while the concentration of CuSO4 was 10 μmol·L-1 for Longjing 24. For the callus induction and plantlet differentiation rates of Kongyu 131 and Dongnong 425, the ideal concentration of uniconazole was 0.50 mg·L-1 and for Longjing 24 was 1.00 mg·L-1.
文摘The Chongqing Tourism and Culture Carnival Gala was held on the afternoon of May 1 0 in the New Zealand National Museum. It was co-hosted by China Cultural Center in New Zealand, the Wellington City Council, Chongqing Culture and Tourism Development Committee and Chongqing Cultural Exchange Center. It was part of the 2019 China Tourism and Culture Week and the China-New Zealand Year of Tourism for the cooperation and communication in culture and tourism.
文摘On January 16,"Happy Chinese New Year" 2019 Launching Ceremony and Press Conference was held in Beijing."Happy Chinese New Year" 2019 has carried out more than 1500 activities in 133 countries and regions in over 40 forms such as temple fairs, exhibitions, performances, lectures, forums and interaction in intangible cultural heritage programs. Taking the opportunity of "Happy Chinese New Year" 2019, Chinese Embassies and Consulates in foreign countries, overseas China culture centers and overseas Chinese tourism offices have explored the integration of culture and tourism and focused on promoting the integration of ideas, with market and local culture. This contributes to showing Chinese New Year culture and telling China's story, making Chinese New Year an international festival shared by the world.