The response of roots and the rhizosphere environment to integrative cultivation practices in paddy rice

Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use efficiency.However,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly understood.In this study,four rice varieties were produced in the field.Each variety was treated with six different cultivation techniques,including zero nitrogen application(0 N),local farmers’practice(LFP),nitrogen reduction(NR),and three progressive ICP techniques comprised of enhanced fertilizer N practice and increased plant density(ICP1),a treatment similar to ICP1 but with alternate wetting and moderate drying instead of continuous flooding(ICP2),and the same practices as ICP2 with the application of organic fertilizer(ICP3).The ICPs had greater grain production and nitrogen use efficiency than the other three methods.Root length,dry weight,root diameter,activity of root oxidation,root bleeding rate,zeatin and zeatin riboside compositions,and total organic acids in root exudates were elevated with the introduction of the successive cultivation practices.ICPs enhanced nitrate nitrogen,the activities of urease and invertase,and the diversity of microbes(bacteria)in rhizosphere and non-rhizosphere soil,while reducing the ammonium nitrogen content.The nutrient contents(ammonium nitrogen,total nitrogen,total potassium,total phosphorus,nitrate,and available phosphorus)and urease activity in rhizosphere soil were reduced in all treatments in comparison with the non-rhizosphere soil,but the invertase activity and bacterial diversity were greater.The main root morphology and physiology,and the ammonium nitrogen contents in rhizosphere soil at the primary stages were closely correlated with grain yield and internal nitrogen use efficiency.These findings suggest that the coordinated enhancement of the root system and the environment of the rhizosphere under integrative cultivation approaches may lead to higher rice production.

Determinants of Tobacco Cultivation and the Associated Impacts of Adoption in Hurungwe District, Zimbabwe

Tobacco is an essential cash crop in Zimbabwe and a strategic livelihood option for hundreds of thousands of rural households. However, the crop is linked to negative environmental, economic, and social impacts. The existing studies on tobacco cultivation in Zimbabwe present contradictory findings on the determinants and impacts of adoption, leaving unanswered questions about the crop’s sustainability impact in the country. This article investigates the determinants of smallholder farmers’ decisions to grow tobacco and the associated impacts of adoption. Random and purposive sampling were used to select 273 household surveys, including tobacco and non-tobacco smallholder farmers, and 56 expert interviews to answer the research questions. We employed regression models alongside expert interviews and document analysis to identify the determinants influencing the decision-making process of smallholder farmers in Zimbabwe regarding tobacco cultivation. Additionally, our investigation aimed to elucidate the perceived impacts associated with the adoption of this agricultural practice. The regression analysis indicated that the farmer’s age, education level, farming experience, family size, household income, and perceived high farm profitability are significant drivers of tobacco adoption. We also discovered divergent and convergent perceptions of the critical impacts of tobacco cultivation. The study highlights the need for proactive multi-stakeholder collaboration and sustainable financial arrangements to address the negative impacts of tobacco production. As the primary stakeholder responsible for regulating and promoting agricultural activities, the Zimbabwean government should provide meaningful financial support, increase access to credit, and ensure better market facilities for alternative crops to reduce the over-dependence on tobacco.

Strategic insights into the cultivation of pancreatic cancer organoids from endoscopic ultrasonography-guided biopsy tissue

BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.

Entrepreneurial Spirit for China in the New Era and Strategies for Cultivation

What type of entrepreneurial spirit is needed for China’s new era,and how can we unlock its innovation potential?Answers to these questions will unleash vital forces to drive China’s high-quality economic development.After reviewing the theories,dimensions,features,and limitations of the entrepreneurial spirit in the Western context,we concluded that the entrepreneurial spirit in China’s new era,which is characterized by patriotism,innovation,trustworthiness,adherence to the law,fulfillment of social responsibilities,and a global horizon,has overcome the limitations that come with self-interested entrepreneurship in the context of Western economics.This is a major step forward in the development of theories on entrepreneurial spirit.In the development context of the new era,this paper argues that the entrepreneurial spirit should become an important force at the individual level to implement the new development concept,foster the new development paradigm,and promote high-quality development in the new stage.This paper provides answers to the question of what type of entrepreneurial spirit China’s new era requires.Finally,this paper presents basic strategies for cultivating entrepreneurial spirit in the context of China’s new era,emphasizing the importance to promote the healthy growth of young entrepreneurs,ensure their business autonomy,develop a multi-tiered capital market,implement the factor market reforms,improve the business climate,and accelerate the digital transition.

The Innovation and Practice of the “Tourism Management + English” Talent Cultivation Model Based on the New Liberal Arts Concept

In the context of globalization, there is an urgent need to cultivate interdisciplinary talents with knowledge of tourism management and proficiency in English. Guided by the New Liberal Arts concept, the “Tourism Management + English” talent cultivation model has emerged. Taking the tourism management talent cultivation of Hainan province as an example, this article analyzes the issues present in traditional tourism management programs and proposes a construction framework that involves conducting in-depth industry research, improving management systems, increasing financial support, implementing strict and standardized management, optimizing talent cultivation plans, highlighting distinctive training features, and strengthening industry services.

Innovation and Practice of the Cultivation Mode of Application-Oriented Undergraduate Talents in Agricultural and Forestry Colleges under the Guidance of the Spirit of the Great Northern Wilderness

Heilongjiang reclamation area has made great progress since its development and construction, among which the agricultural and forestry colleges in China have played a key role in the input of talents for the production of reclamation area, and the spirit of the Great Wilderness has important strategic significance for the cultivation of agricultural and forestry talents. Taking Heilongjiang Bayi Agricultural University as an example, this paper analyzes the ways of the Great Northern Wilderness Spirit for cultivating application-oriented undergraduate talents in agricultural and forestry colleges, improving the curriculum system of undergraduate talents through innovation, strengthening the cultivation of students’ innovative ability, leading students’ scientific research and academic level, and enhancing students’ practical ability. To innovate the cultivation mode of application-oriented undergraduate talents in agricultural and forestry colleges under the guidance of the spirit of the Great Northern Wilderness, and to provide a reference mode for the cultivation of undergraduate talents in agricultural and forestry colleges in China.

Farmers’ Knowledge and Awareness towards the Use of Agrochemicals in Vegetable Cultivation, Fako Cameroon

Agrochemicals are contemporary, omnipresent tool used in vegetable cultivation. Farmers’ knowledge and awareness of the proper usage of agrochemicals are critical for mitigating the negative effects on human health. This cross-sectional study was aimed at assessing the usage knowledge, risk awareness of toxicological and chemical classes, proper handling and use practices for agrochemicals homologated for use in vegetable farming, and the occurrence of health-related symptoms as a result of exposure among these farmers. The study included 93 vegetable growers from agricultural hotspot towns in Fako, southwest Cameroon. The field study, ran from November 2021 to December 2023, using a questionnaire to collect information on farmers demographic, and their knowledge of pesticide classes, and the related risk of associated with the handling of agrochemicals. Results show that all vegetable farmers, particularly those engaged in agribusiness, employ pesticide inputs to maximize production. Six pesticides, two fertilizer types, and one unknown substance were identified. While 23 active compounds were found, the most utilized were abamectin, emamectin (10.46%), dimethoate (9.30%,) and ethoprophos (8.13%). Two active chemicals, dimethoate and methalaxyl, are illegal yet remain in circulation. Toxicological classes I and II, with the greatest harmful effect on human health, were the most commonly utilized (64.27%). Thirty-nine percent of farmers never use personal protection equipment when working with agrochemicals, demonstrating a significant gap in knowledge and awareness of agrochemicals and their various applications and handling procedures in the field. The government should implement an intensive specialized educational program for on-field farmers with incentives in order to promote sustainable agriculture methods that ensure environmental and human safety.

Research on the Cultivation of Aesthetics among Art Students under the New Era Background

Through understanding the history and current situation of aesthetic education in China,this paper explains some misunderstandings in the aesthetics of contemporary university art students,such as the insufficiency of aesthetic value and ability,as well as the inadequacy of students’creativity due to traditional art education.It is also pointed out that the cultivation of core literacy of art students is a multidimensional and comprehensive process,and these problems need to be solved by schools,teachers,and students,so as to strengthen the inner aesthetic education of students and improve their aesthetic awareness and ability.Aesthetic education is not only an important part of the art classroom but also a key part of fostering students’all-round development and enhancing their humanistic literacy.It is necessary to cultivate students’observation,imagination,image-thinking ability,and creativity,so that they can become artistic talents with independent thinking and innovation.

Research on Foreign Language Talent Cultivation Mode Based on the Concept of Language Security

Language security is an important part of national security,and in the current complex international environment,the issue of language security is becoming more and more prominent and has become an unignorable part of national security strategy.As the bridge and link of international communication,foreign language talents’language skills and the level of national security awareness directly affect our country’s international image and the effect of international communication.Therefore,the establishment of a foreign language talent cultivation mode based on the concept of language security is not only an important way to improve the quality of foreign language education but also a realistic need to safeguard national security and promote international exchanges.Starting from the influence of the language security concept on foreign language talent cultivation,this paper analyzes the foreign language talent cultivation mode based on the language security concept,with a view to providing new ideas and methods for the development of foreign language education.

Exploring the Role of Narrative Medicine in the Cultivation of Humanistic Spirit among Respiratory Medicine Interns

Objective:To explore the role of narrative medicine in cultivating the humanistic spirit of respiratory medicine interns.Methods:Thirty interns were selected,all of whom were received by the respiratory department of our hospital from September 2023 to June 2024 and underwent narrative medicine teaching.Scores on narrative medicine knowledge,empathy ability,humanistic care ability,and teaching recognition were compared before and after the internship.Results:Compared to before the internship,the interns’scores on narrative medicine knowledge were significantly higher after the internship,as were their empathy and humanistic care scores(P<0.05).Among the 30 interns,25 expressed interest in narrative medicine,and 29 were willing to continue with the narrative medicine teaching model.Conclusion:The application of narrative medicine in respiratory medicine internship teaching is highly effective.It cultivates the humanistic spirit of interns,improves their empathy and humanistic care abilities,and is worthy of wider implementation.

Research on the Generation of Professional Skill Modules for Talent Cultivation in the Landscape Architecture Profession for Industry-Education Integration of Small and Medium-Sized Enterprises

Due to industry characteristics or regional limitations,many students in certain majors tend to seek employment in small and medium-sized enterprises(SMEs).SMEs face certain challenges in implementing industry-education integration,and there is a lack of mature methods in specific implementation processes and talent cultivation plan generation.Taking the landscape architecture profession as an example,this paper conducts a correlation analysis and cross-analysis of the industry-required professional skills based on surveys of over 300 industry practitioners employed in SMEs.It provides professional skill cultivation modules based on market feedback.This research process and analysis method have certain reference significance for the rapid production of adaptive talent cultivation professional skill modules in other industries targeting SMEs.

Research on the Reform and Practice of Blended Teaching in “Etiquette and Cultural Cultivation” Courses: Taking Communication University of Zhejiang as an Example

Taking Communication University of Zhejiang as an example,this paper summarizes the overview of the“Etiquette and Cultural Cultivation”course and analyzes the problems existing in the teaching of the course.It also explores the ideas and practical strategies of the teaching reform of the course and sums up the innovative results achieved,to provide a reference for relevant teachers.

The Reform and Practice of Multicultural Cultivation Mode of Art Talents in Colleges and Universities Under the Strategy of “Three Highs and Four New”

As an important link in the development of modern quality education,art education is one of the methods to guide students to have a diversified vision.Under the background of multiculturalism,we need to explore the connotation and value of art education with the concept of“three highs and four new,”so that students can realize interdisciplinary and cross-cultural cognition through an immersive experience and practical operation in a wide range of multicultural situations,and to develop the knowledge of art education with the concept of“three highs and four new.”Based on the multicultural background,this paper focuses on the integration of multiculturalism into the reform practice of“student-centered”in colleges and universities,so as to cultivate art talents adapted to the multicultural background,in order to achieve the great mission of“three highs and four new”and better lead the innovative development of art education in colleges and universities.

Discussion on the Talent Cultivation Strategy for Interior Design in Vocational Colleges from the Perspective of Industry-Education Integration

To further enhance the effectiveness of talent cultivation for interior design in vocational colleges,it is necessary to vigorously promote the construction of an industry-education integration model.Through this model,the roles of both enterprises and schools can be leveraged to jointly facilitate the continuous improvement of students’professional abilities and practical skills,providing a steady stream of high-quality talents for the development of the interior design field.Therefore,this paper analyzes the current issues in interior design talent cultivation in vocational colleges from the perspective of industry-education integration and proposes corresponding improvement measures.

The Impact of The Three-Dimensional Cultivation Model on the Development of the Suzhou Tea Industry:Estimating Value of Output Per Acre and Diversified Sales Models

This article explores the impact of the three-dimensional cultivation mode on the development of the Suzhou tea industry,focusing on the diversified estimation of the value of output per acre and sales mode.It introduces the history and traditional cultivation practices of tea in Suzhou,as well as the current challenges and problems faced by the industry.An in-depth analysis was conducted on the overview and improvement plans of the three-dimensional cultivation mode,covering relevant technical methods.Based on this analysis,the impact of the three-dimensional cultivation on the value of output per acre was studied and predicted.Its potential and advantages were explored and compared with the effectiveness of traditional cultivation models.Additionally,the impact of the three-dimensional cultivation mode on sales was analyzed,examining its market adaptability and competitiveness,as well as its advantages in expanding sales channels and market coverage.The study also focused on the promoting effect of diversified sales models on the Suzhou tea industry,including direct consumption market development,tea processing product development and promotion,and the integration of tea culture and the tourism industry.To ensure sustainable development,the article evaluates the environmental impact,economic feasibility,social benefits,and farmer benefits of the three-dimensional cultivation model.Finally,the prospects for the development of the Suzhou tea industry were discussed,and the positioning and response strategies of the threedimensional cultivation model were proposed.

Exploration of the Talent Cultivation Path of New Higher Vocational Compound Childcare Integration

With the continuous development of society,people’s concept of early childhood education is also gradually upgrading.In the report of the 19th National Congress of the Communist Party of China(CPC),“childcare”is listed as one of the seven national key livelihood tasks,and the model of“integration of childcare and early childhood”is gradually being widely used in infant and toddler education.The application of this model not only promotes the development of infant and toddler education but also brings new challenges to the training of talents specialized in childcare in higher vocational colleges.Higher vocational colleges and universities should adjust the talent training mode according to the requirements of the childcare integration mode,so as to build a high-quality skilled personnel team and promote the further development of infant and toddler education.

The Anylasis of Fitness on Maize Cultivation Based on RS and GIS

Maize is one of our country quite important grain crops,its height output is an important pillar ensure that grain of our country is safe,if speedy science ascertaining that maize expediency area cultivating,it can provide the most reliable decision-making reference to jobs such as agricultural regionalization,crop layout,accurate agriculture of essence.The article carries out a maize cultivating expediency research make use of the minute RS and GIS means,it can guarantee the results is timeliness and scientificalness.

Effects of Cultivation Methods on Yield Formation and Photosynthetic Characteristics of Zhuliangyou 4024 and H You 159 as Doublecropping Rice

To explore the high-yield cultivation methods of Zhuliangyou 4024 and H You 159 when planted as double-cropping rice, the effects of two cultivation meth- ods, i.e., seedling broadcasting and transplanting, were studied on SPAD value, photosynthetic rate, grain yield and yield components. The results showed that the seedling broadcasting cultivation was conducive to Zhuliangyou 4024 and H You 159 to obtain higher yield. The higher chlorophyll content and photosynthetic rate and their slower decrease of flag leaf after full heading of the seedling broadcasting cultivation were thought to be the physiological basis of high yield.

Effects of Planting Density, Duration of Disclosing Plastic Film and Nitrogen Fertilization on the Growth Dynamics of Rapeseed under No-tillage Cultivation

[ Objective] This study was to understend the optimized combination of planting density, duration of disclosing plastic film and nitrogen fertilization under no-tillage cultivation. [ Method] Quadratic polynomial regression and saturated D-optimal design were employed to investigate the effects of planting density, duration of disclosing plastic film and nitrogen fertilization on the dynamics growth of rapeseed under no-tillage cultivation.[ Result] Within the experimental range, the growth dynamics of no-tillage cultivated rapeseed assumed a rise-fall tend. For the effects to the growth dynamics of no-tillage cultivated rapeseed, nitrogen application amount was higher than planting density and duration of disclosing plastic film. The interaction effect between planting density and duration of disclosing plastic film was higher than that between nitrogen application amount and planting density, and between nitrogen application amount and duration of disclosing plastic film. [ Conclusion] The optimized combination of these factors for dynamic growth of rapeseed under no-tillage cultivation was determined to be： planting density of per hectare 154 925 individuals, duration of disclosing plastic film of 110 d, nitrogen application amount of 315 kg/hm^2.

On the Cultivation & Maintenance of Gardening Plants in Residential Areas of Liuzhou City

According to the analysis of the specific condition of green space of residential area in Liuzhou,effective suggestions to the design,cultivation and maintenance were put forward.