Effects of electro-acupuncture on 5-HT,NOS and gastric mucosa in stress rats

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Protective effect of Weikang decoction and partial ingredients on model rat with gastric mucosa ulcer

AIM:To investigate the protective mechanisms of Weikang (WK) decoction on gastric mucosae.METHODS: Ninety rats were randomly divided into nine groups of 10 each, namely group, model group, group with large WK dosage, group with medium WK dosage,group with small WK dosage, group with herbs of jianpiyiqi (strengthening the spleen and replenishing qi), group with herbs of yangxuehuoxue (invigorating the circulation of and nourishing the blood), group with herbs of qingrejiedu (clearing away the heat-evils and toxic materials), group with colloidal bismuth pectin (CBP) capsules. According to the method adopted by Yang Xuesong, except normal control group, chronic gastric ulcer was induced with 100% acetic acid. On the sixth day after moldmaking, WK decoction was administered, respectively at doses of 20,10 and 5 g/kg to rats of the WK groups, or the groups with herbs of jianpiyiqi, yangxuehuoxue and qingrejiedu,10 mL/kg was separately administered to each group every day. For the group with CBP capsules, medicine was dissolved with water and doses 15 times of human therapeutic dose were administered (10 mL/kg solution containing 0.35% CBP). Rats of other groups were fed with physiological saline (10 mL/kg every day).Administration lasted for 16 d. Rats were killed on d 22after mold making to observe changes of gastric mucosa.The mucus thickness of gastric mucosa surface was measured. Levels of epidermal growth factor (EGF) in gastric juice, nitric oxide (NO) in gastric tissue, endothelin (ET) in plasma, superoxide dismutase (SOD) in plasma,malondialdehyde (MDA) in plasma and prostaglandin I2(PGI2) were examined.RESULTS: Compared with control group, ulceration was found in gastric mucosa of model group rats. The mucus thickness of gastric mucosa surface, the levels of EGF,NO, 6-K-PGF1α and SOD decreased significantly in the model group (EGF: 0.818±0.18 vs2.168±0.375, NO:0.213±0.049 vs 0.601±0.081, 6-K-PGF1α: 59.7±6.3 vs 96.6±8.30, SOD: 128.6±15.0 vs196.6±35.3, P＜0.01), the levels of ET (179.96±37.40 vs 46.64±21.20, P＜0.01) and MDA (48.2±4.5 vs 15.7±4.8, P＜0.01) increased. Compared with model group, the thickness of regenerative mucosa increased, glandular arrangement was in order, and cystic dilative glands decreased, while the mucus thic kness of gastric mucosa surface increased (20 g/kg WK: 51.3±2.9vs23.2±8.4, 10 g/kg WK: 43.3±2.9 vs 23.2±8.4, 5 g/kg WK:36.1±7.2 vs 23.2±8.4, jianpiyiqi: 35.4±5.6 vs 23.2±8.4,yangxuehuoxue: 33.1±8.9 vs 23.2±8.4, qingrejiedu:31.0±8.0 vs 23.2±8.4 and CBP: 38.2±3.5 vs 23.2±8.4,P＜0.05-0.01). The levels of EGF (20 g/kg WK: 1.364±0.12vs 0.818±0.18, 10 g/kg WK: 1.359±0.24 vs 0.818±0.18,5 g/kg WK: 1.245±0.31 vs 0.818±0.18, jianpiyiqi: 1.025±0.45 vs 0.818±0.18, yangxuehuoxue: 1.03±0.29 vs 0.818±0.18, qingrejiedu: 1.02±0.47 vs 0.818±0.18 and CBP:1.237±0.20 vs 0.818±0.18, P＜0.05-0.01), NO (20 g/kg WK:0.480±0.026 vs 0.213±0.049, 10 g/kg WK: 0.390±0.055vs 0.213±0.049, 5 g/kg WK: 0.394±0.026 vs 0.213±0.049,jianpiyiqi: 0.393±0.123 vs 0.213±0.049, yangxuehuoxue:0.463±0.077 vs 0.213±0.049, qingrejiedu: 0.382±0.082vs 0.213±0.049 and CBP: 0.395±0.053 vs 0.213±0.049,P＜0.05-0.01), 6-K-PGF1α (20 g/kg WK: 86.8±7.6 vs 59.7±6.3, 10 g/kg WK: 77.9±7.0 vs 59.7±6.3, 5 g/kg WK:70.0±5.4 vs 59.7±6.3, jianpiyiqi: 73.5±12.2 vs 59.7±6.3,yangxuehuoxue: 65.1±5.3 vs 59.7±6.3, qingrejiedu:76.9±14.6 vs 59.7±6.3, and CBP: 93.7±10.7 vs 59.7±6.3,P＜0.05-0.01) and SOD (20 g/kg WK: 186.4±19.9 vs 128.6±15.0, 10 g/kg WK: 168.2±21.7 vs 128.6±15.0, 5 g/kg WK: 155.6±21.6 vs 128.6±15.0, jianpiyiqi: 168.0±85.3vs 128.6±15.0, yangxuehuoxue: 165.0±34.0 vs 128.6±15.0,qingrejiedu: 168.2±24.9 vs 128.6±15.0, and CBP: 156.3±18.1vs 128.6±15.0, P＜0.05-0.01) significantly increased. The levels of ET (20 g/kg WK: 81.30±17.20 vs 179.96±37.40,10 g/kg WK: 83.40±25.90 vs 179.96±37.40, 5 g/kg WK:93.87±20.70 vs 179.96±37.40, jianpiyiqi: 130.67±43.66vs 179.96±37.40, yangxuehuoxue: 115.88±34.09 vs 179.96±37.40, qingrejiedu: 108.22±36.97 vs179.96±37.40,and CBP: 91.96±19.0 vs 179.96±37.40, P＜0.01) and MDA (20 g/kg WK: 21.6±7.4 vs48.2±4.5, 10 g/kg WK:32.2±7.3 vs 48.2±4.5, 5 g/kg WK: 34.2±6.2 vs 48.2±4.5,jianpiyiqi: 34.9±13.8 vs 48.2±4.5, yangxuehuoxue:35.5±16.7 vs 48.2±4.5, qingrejiedu: 42.2±17.6 vs 48.2±4.5, and CBP: 30.1±6.1 vs 48.2±4.5, P＜0.05-0.01)obviously decreased. The 20 g/kg WK group was better than 10 g/kg (the mucus thickness: 51.3±2.9 vs43.3±2.9,NO: 0.480±0.026 vs 0.390±0.055, SOD: 186.4±19.9 vs 168.2±21.7, P＜0.01) and 5 g/kg (the mucus thickness:51.3±2.9 vs 36.1±7.2, NO: 0.480±0.026 vs 0.394±0.026,SOD: 186.4±19.9 vs 155.6±21.6, P＜0.01) groups and CBP group (the mucus thickness: 51.3±2.9 vs 38.2±3.5, NO:0.480±0.026 vs 0.395±0.053, SOD: 186.4±19.9 vs 156.3±18.1, P＜0.01) in the mucus thickness, NO and SOD levels and better than 10 g/kg (86.8±7.6 vs 77.9±7.0,P＜0.05) and 5 g/kg (86.8±7.6 vs 70.0±5.4, P＜0.05)groups in 6-K-PGF1α level, 10 g/kg WK group was better than 5 g/kg WK (the mucus thickness: 43.3±2.9 vs 36.1±7.2,P＜0.01, SOD: 168.2±21.7 vs 155.6±21.6, P＜0.05) and CBP groups (the mucus thickness: 43.3±2.9 vs 38.2±3.5,P＜0.01, SOD: 168.2±21.7 vs 156.3±18.1, P＜0.05) in the mucus thickness and SOD level. In compound group,jianpiyiqi group, yangxuehuoxue group, qingrejiedu group,the level of ET was decreased, NO contents were increased in gastric tissue of ulcers in rats.CONCLUSION: WK decoction and separated recipes have significantly protective effect on ethanol-induced gastric mucosal injury. They can increase the content of EGF in gastric juice, PGI2 SOD in plasma and NO in gastric tissues,thicken the mucus on the gastric mucosa, and decrease the impairing factor MDA, ET in plasma.

PROTECTIVE EFFECT OF MOXIBUSTION AT SHENQUE(RN 8)ON GASTRIC MUCOSA

Male S.D rats weighing 200g were used(as experimental animals).Moxibus-tion at Shenque(RN 8)was performed for 15 minutes each day.The course lasted for weeks.In ratsundergoing oral infusion of taurocholate,index of gastric mucosal injury were 9.0±6.1 and 4.6±2.5 in two and four week groups respectively.Compared with control group(index:16.8±7.6),Pwas less than 0.05 and 0.01.This indicated that moxibustion had protective effect on gastric mucosalinjury.Time-effect relationship also existed.In experiment of analysis about mechanism of moxibustion,we found that moxibustion.1.stim-ulated the secretion of gastric mucus(P【0.001);2.increased the PGE 2 content in gastric mucosa(P【0.05);3.increased the number of β-endorphin-like immunoactive cells in gastric mucosa(P【0.05);4.enhanced the transformation of lymphocytes(P【0.01);5.reinforced the function of an-tibody-producing cells in the spleen.The results suggested that protective effect of moxibustion might result from the increase in mu-cus,the PGE 2 and the endogenous β-endorphin.It might also be related to the reinforcing effect ofmoxibustion on transformation of lymphocytes and antibody-producing function of spleen cells.

Molecular forms of trefoil factor 1 in normal gastric mucosa and its expression in normal and abnormal gastric tissues

AIM: To study the molecular forms of trefoil factor 1 (TFF1) in normal gastric mucosa and its expression in normal and abnormal gastric tissues (gastric carcinoma, atypical hyperplasia and intestinalized gastric mucosa) and the role of TFF1 in the carcinogenesis and progression of gastric carcinoma and its molecular biological mechanism underlying gastric mucosa protection. METHODS: The molecular forms of TFF1 in normal gastric mucosa were observed by Western blot. The expression of TFF1 in normal and abnormal gastric tissues (gastric carcinoma, atypical hyperplasia and intestinalized gastric mucosa) was also assayed by immunohistochemical method. The average positive AO was estimated by Motic Images Advanced 3.0 software. RESULTS: Three patterns of TFF1 were found in normal gastric mucosa: monomer, dimmer, and TFF1 compound whose molecular weight is about 21 kDa. The concentration of TFF1 compound was the highest among these three patterns. TFF1 was expressed mainly in epithelial cytoplasm of the mucosa in gastric body and antrum, especially around the nuclei. The closer the TFF1 to the lumen, the higher the expression of TFF1. The expression of TFF1 in peripheral tissue of gastric carcinoma (0.51 ± 0.07) was higher than that in normal gastric mucosa (0.44 ± 0.06, P < 0.001). The expression of TFF1 in gastric adenocarcinoma was positively related to the differentiation of adenocarcinoma. The lower the differentiation of adenocarcinoma was, the weaker the expression of TFF1. No TFF1 was expressed in poorly- differentiated adenocarcinoma. The expression of TFF1 in moderately-well differentiated adenocarcinoma (0.45 ± 0.07) was a little lower than that in normal mucosa (P > 0.05). The expression of TFF1 in gastric mucosa with atypical hyperplasia (0.57 ± 0.03) was significantlyhigher than that in normal gastric mucosa (P < 0.001). No TFF1 was expressed in intestinalized gastric mucosa. There was no statistically significant difference between the expressions of TFF1 in gastric mucosa around the intestinalized tissue (0.45 ± 0.07) and normal gastric mucosa (P > 0.05). CONCLUSION: TFF1 is expressed mainly in epithelial cytoplasm of the mucosa in gastric body and antrum. Its main pattern is TFF1 compound, which may have a greater biological activity than monomer and dimer. The expression of TFF1 in peripheral mucosa of gastric ulcer is higher than that in mucosa 5 cm beyond the ulcer, indicating that TFF1 plays an important part in protection and restitution of gastric mucosa. The expression of TFF1 is increased in peripheral tissues of gastric carcinoma and gastric mucosa with atypical hyperplasia, but is decreased in cancer tissues, implying that TFF1 may be related to suppression and differentiation of carcinoma. The weaker expression of TFF1 in poorly-differentiated carcinoma may be related to the destruction of glands and cells in cancer tissues and the decrease in secretion of TFF1.

Relationship between trefoil factor 1 expression and gastric mucosa injuries and gastric cancer

AIM: To determine whether trefoil factor 1 (TFF1) is associated with mucosa healing and carcinoma suppression,we assess the expression of trefoil factor 1 in normal and pathologic gastric mucosa.METHODS: TFFI in normal and pathologic gastric mucosa was assessed by immunohistochemical method, and the average positive A was estimated by Motic Images Advanced 3.0 software.RESULTS: Increased TFF1 was detected in gastritis,gastric ulcer and duodenal ulcer compared with normal mucosa. The same result could be seen in multiple and compound ulcer compared with simple ulcer. There was no significant difference between gastric ulcer and duodenal ulcer, gastritis and simple ulcer respectively.Increased TFF1 was detected in the peripheral mucosa of the gastric adenocarcinoma compared with normal mucosa. The expression of TFF1 in gastric adenocarcinoma was related to the differentiation of adenocarcinoma. The lower the differentiation of adenocarcinoma, the weaker the expression of TFF1. There was no TFF1 expressed in low-differentiated adenocarcinoma. The expression of TFF1 in middle and highly differentiated adenocarcinoma was a little lower than that in normal mucosa. But there was no significant difference. No TFF1 was assessed in esophageal squamous carcinoma and peripheral tissue. There was no significant difference between male and female.CONCLUSION: The expression of TFF1 was higher in gastritis and peptic ulcer than that in normal mucosa,and was also higher in multiple and compound ulcer than in simple ulcer. It seems that TFF1 plays a role in gastric mucosa protection and epithelial restitution. Increased expression of TFF1 in peripheral tissue suggests that TFF1 is associated with mechanism of carcinoma suppression and differentiation. Decreased expression of TFFI in carcinoma and its relativity to the differentiation suggests that TFF1 is related to gland and cell destruction of carcinoma.

Association of high expression in rat gastric mucosal heat shock protein 70 induced by moxibustion pretreatment with protection against stress injury

AIM:To study the effect of moxibustion on Zusanli or Liangmeng point on gastric mucosa injury in stress-induced ulcer rats and its correlation with the expression of heat shock protein 70 (HSP70). METHODS:Sixty healthy SD rats (30 males,30 females) were divided into control group,injury model group,Zushanli point group,Liangmeng point group. Stress gastric ulcer model was induced by binding cold stress method. Gastric mucosa ulcer injury (UI) index was calculated by Guth method. Gastric mucosa blood flow (GMBF) was recorded with a biological signal analyzer. Protein content and gene expression in gastric mucosal HSP70 were detected by immunohistochemistry (IHC) and reverse transcription polymerase chain reaction (RT-PCR). Thiobarbital method was used to determine malondialdehyde (MDA) content. Gastric mucosal endothelin (ET) and prostaglandin E2 (PGE2) were analyzed by radioimmunoassay. RESULTS:High gastric mucosal UI index,high HSP70 expression,low GMBF and PGF2,elevated MDA and ET were observed in gastric mucosa of rats subjected to cold stress. Moxibustion on Zusanli or Liangmeng point decreased rat gastric mucosal UI index,MDA and ET. Conversely,the expression of HSP70,GMBF,and PGE2 was elevated in gastric mucosa after pretreatment with moxibustion on Zusanli or Liangmeng point. The observed parameters were significantly different between Zusanli and Liangmeng points. CONCLUSION:Pretreatment with moxibustion on Zusanli or Liangmeng point protects gastric mucosa against stress injury. This protection is associated with the higher expression of HSP70 mRNA and protein,leading to release of PGE2 and inhibition of MDA and ET,impairment of gastric mucosal index.

Nutritional factors and gastric cancer in Zhoushan Islands, China

AIM: To investigate the association between nutrient intakes and high incidence rate of gastric cancer among residents in Zhoushan Islands.METHODS: A frequency-matched design of case-control study was used during the survey on dietary factors and gastric cancer in Zhoushan Islands, China. A total of 103 cases of gastric cancer diagnosed in 2001 were included in the study and 133 controls were randomly selected from the residents in Zhoushan Islands. A food frequency questionnaire was specifically designed for the Chinese dietary pattern to collect information on dietary intake. A computerized database of the dietary and other relative information of each participant was completed. Total calories and 15 nutrients were calculated according to the food composition table and their adjusted odds ratios (ORs) and 95% confidence intervals (CIs) were estimated by gender using unconditional logistic regression models.RESULTS: High intakes of protein, saturated fat, and cholesterol were observed with the increased risk of gastric cancer particularly among males (ORQ4 vsQ1 were 10.3, 3.24, 2.76 respectively). While carbohydrate was a significant high-risk nutrient (ORQ4 vsQ1 = 14.8; Pfor linear trend = 0.024) among females. Regardless of their gender, the cases reported significantly higher daily intake of sodium mainly from salts. As to the nutrients of vitamins A and C, an inversed association with the risk of GC was found. Baseline characteristics of participants were briefly described.CONCLUSION: The findings from this study confirm the role of diet-related exposure in the etiology of gastric cancer from the point of view of epidemiology. An increased risk of gastric cancer is associated with high intakes of protein, saturated fat, cholesterol and sodium,while consumption of polyunsaturated fat, vitamin A and ascorbic acid may have a protective effect against gastric cancer.

解酒护肝胶囊对大鼠酒精性肝损伤保护作用研究

目的评价解酒护肝胶囊对酒精性肝损伤的防治作用以及增强酒精代谢功效研究。方法各实验组灌胃给予不同浓度受试样品后,建立酒精性肝损伤及急性酒精中毒模型。测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、谷氨酰氨基转移酶(GGT)、乙醇和乙醛含量;检测肝脏中三酰甘油(TG)、超氧化物歧化酶(SOD)水平;HE染色观察肝脏和胃组织的病理变化。结果与模型组比较,解酒护肝胶囊中剂量组能显著降低血清中ALT含量(P<0.05),中、高剂量组能显著降低AST和GGT含量(P<0.01),低、中、高剂量组均能显著降低肝脏组织中TG含量(P<0.01),高剂量组显著提高SOD水平(P<0.01)。肝脏组织病理切片显示,模型组大鼠肝细胞大小不均,肝索排列紊乱,有较多小泡性脂肪空泡,同时伴有炎症浸润、细胞坏死,各剂量给药组均可不同程度改善大鼠肝脏组织病变情况;与模型组比较,解酒护肝胶囊高剂量组在醉酒后8 h内乙醇和乙醛含量均显著下降(P<0.01)。解酒护肝胶囊低、高剂量组肝脏和胃组织的病变情况均有所缓解。结论解酒护肝胶囊可抑制氧化应激反应,降低脂质过氧化,加速酒精代谢,对肝组织和胃黏膜具有一定的保护作用。

瑞巴派特联合三联疗法治疗老年消化性胃溃疡的临床效果分析

目的探讨老年消化性胃溃疡采用瑞巴派特联合三联疗法治疗的临床效果。方法选择丹徒区人民医院2020年4月至2023年10月收治的老年消化性胃溃疡患者94例,按随机数表法分为两组,各47例。对照组采用常规三联疗法,观察组在此基础上加用瑞巴派特。对比两组临床治疗效果、症状改善时间、胃黏膜形态学与不良反应。结果较之对照组,观察组治疗总有效率更高,差异有统计学意义(P<0.05)。较之对照组,观察组恶心、嗳气、反酸及腹痛症状改善时间均更短,差异有统计学意义(P<0.05)。观察组治疗后胃黏膜厚度、腺体密度评分均较对照组低,差异有统计学意义(P<0.05)。两组不良反应总发生率对比,差异无统计学意义(P>0.05)。结论采用瑞巴派特联合三联疗法治疗老年消化性胃溃疡的效果确切、安全性高,对患者症状、胃黏膜形态的改善效果明显。

Experimental study on mechanism and protection of stress ulcer produced by explosive noise

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沉香汤治疗胃脘痛疗效及对中医证候、胃黏膜的影响

目的 研究沉香汤联合常规西医治疗胃脘痛患者的临床疗效,及其对患者中医证候和胃黏膜的影响。方法 选取惠州市中心人民医院2020年9月1日—2021年3月31日治疗的120例胃脘痛患者,将其按随机数字表法分为观察组和对照组,每组60例,对照组患者接受常规西医药物治疗,观察组患者在对照组基础上联合沉香汤治疗,比较两组患者的临床疗效、中医证候积分、胃黏膜情况以及不良反应等。结果 观察组患者治疗总有效率显著高于对照组患者[91.67%(55/60)vs 73.33%(44/60)](P<0.05);接受治疗后,两组患者各中医证候积分均显著降低(P<0.05),且观察组患者治疗后各中医证候积分均显著低于对照组(P<0.05);观察组患者治疗前后胃黏膜糜烂、出血及萎缩分别减少7例、17例、18例,改善率分别为70.00%、85.00%、87.50%,对照组患者治疗前后胃黏膜糜烂、出血及萎缩分别减少5例、16例、12例,改善率分别为55.56%、69.57%、63.64%,两组胃黏膜糜烂、出血及萎缩改善率差异无统计学意义(P>0.05);两组患者治疗期间不良反应发生率差异无统计学意义[6.67%(4/60)vs 5.00%(3/60)](P>0.05)。结论 沉香汤联合常规西医治疗胃脘痛患者临床疗效和安全性较好,可有效改善患者症状及胃黏膜情况。

芍药甘草汤对大鼠酒精性胃溃疡的保护作用及其可能机制研究

目的:本研究旨在评估芍药甘草汤(SGD)对大鼠酒精性胃溃疡(GU)的保护作用及其可能机制,以期为临床治疗提供参考。方法:采用单次灌胃10 mL/kg 75%乙醇建立大鼠胃溃疡模型,对胃组织进行组织病理学检查。用双抗体夹心法测定胃组织中肿瘤坏死因子-α(TNF-α)、表皮生长因子(EGF)、前列腺素E2(PGE2)、超氧化物歧化酶(SOD)和血浆硫代巴比妥酸反应物质(TBARS)水平。应用网络药理学研究方法,进一步研究SGD治疗GU的可能机制。结果:组织学特征显示,酒精性GU大鼠的胃组织在整个胃黏膜层均有明显的损伤,而SGD治疗组大鼠的胃黏膜损伤明显减轻。标志物检测显示,SGD(9.90 g/kg)组和SGD(19.80 g/kg)组TNF-α的水平显著下降(分别为模型组的87.17%和86.50%,P<0.01)。SGD(9.90 g/kg)组和SGD (19.80 g/kg)组PGE2水平均显著高于模型组(分别为模型组的172.40%和220.86%,P<0.01)。与模型组比较,SGD(19.80 g/kg)组SOD水平均显著升高(为模型组的162.13%,P<0.01)。而在脂质过氧化方面,SGD(9.90 g/kg)组、SGD(19.80 g/kg)组TBARS水平显著下降(分别为模型组的78.66%、71.83%,P<0.01)。结论:SGD对乙醇所致GU有改善作用。此外,本研究还揭示了SGD治疗GU的多组分、多靶点、多途径的特点,为进一步的药物开发研究提供了基础。

艳山姜总黄酮改善无水乙醇致胃黏膜细胞损伤的作用及机制

目的探讨艳山姜总黄酮通过微RNA-146a-5p(miR-146a-5p)对无水乙醇诱导的胃黏膜细胞损伤的改善作用及潜在机制。方法以人胃黏膜细胞GES-1为对象,以无水乙醇诱导建立急性胃溃疡细胞模型,在考察不同质量浓度艳山姜总黄酮对细胞活力影响及筛选作用浓度的基础上,检测细胞中miR-146a-5p的相对表达量,细胞中肿瘤坏死因子受体关联因子6(TRAF6)、核因子κB p65(NF-κB p65)、肿瘤坏死因子α(TNF-α)蛋白的表达水平,以及细胞上清液中白细胞介素1β(IL-1β)、IL-6、前列腺素E2(PEG2)水平;验证miR-146a-5p与TRAF6的靶向关系,并观察过表达miR-146a-5p、敲减TRAF6对模型细胞上清液中IL-1β、IL-6、PEG2水平的影响,以及敲减miR-146a-5p对艳山姜总黄酮抗胃溃疡作用的影响。结果与空白组比较,模型组细胞中miR-146a-5p的相对表达量和上清液中PGE2水平均显著降低(P<0.01),细胞中TRAF6、NF-κB p65、TNF-α蛋白的表达水平和上清液中IL-1β、IL-6水平均显著升高(P<0.01)。与模型组比较,模型+艳山姜总黄酮(60 mg/L)组细胞中miR-146a-5p的相对表达量和上清液中PGE2水平均显著升高(P<0.01),细胞中TRAF6、NF-κB p65、TNF-α蛋白的表达水平和上清液中IL-1β、IL-6水平均显著降低(P<0.05或P<0.01)。miR-146a-5p与TRAF6存在靶向关系且呈负相关;过表达miR-146a-5p或敲减TRAF6后,细胞上清液中IL-1β、IL-6水平均显著降低,PGE2水平显著升高(P<0.05)。敲减miR-146a-5p后,艳山姜总黄酮作用下的细胞上清液中IL-1β、IL-6水平和细胞中TRAF6蛋白的表达水平均显著升高,PGE2水平显著降低(P<0.05)。结论艳山姜总黄酮对无水乙醇致胃黏膜细胞损伤有一定的改善作用,其机制可能与上调miR-146a-5p的表达、抑制TRAF6的表达,进而抑制相关炎症因子的分泌有关。

分析内镜黏膜下剥离术治疗30例早期胃癌的临床疗效

目的对早期胃癌患者采用黏膜下剥离术进行临床治疗进行回顾性分析与讨论。方法对于本院2020年1月进行早期胃癌治疗的30例患者采用内镜黏膜下剥离术进行临床手术治疗,从术前、术后患者的病情变化发展进行讨论与分析,并对其进行为期3年的随访工作,探讨术后胃癌复发率,对各项观察指标进行统计学分析。其中观察指标主要包括对并发症情况、血清因子水平、TGFβ1蛋白及幽门螺杆菌-lg G水平、复发情况以及生存质量进行数据统计分析,对其术后治疗情况及其早期胃癌的病情影响因素进行讨论与分析。结果针对30例早期胃癌患者进行内镜黏膜下剥离术,其术后并发症发生率仅为10%,且血清CA19-9、CEA、CA12-5、miR-125水平较术前显著降低(P<0.05)。TGFβ1蛋白、幽门螺杆菌-lgG水平也较术前显著降低(P<0.05)。在术后胃癌复发率的调查中,仅有2例患者于术后三年内复发胃癌,其复发率为6.67%,且术后患者生活质量较术前均得到了显著改善(P<0.05)。结论针对早期胃癌患者的病症对病灶进行内镜黏膜下剥离术的手术治疗,能够有效降低患者并发症的发生率,有助于进行长期治疗,提高预后治疗效率,具有良好治疗效果。

200∶1库拉索芦荟凝胶冻干粉对大鼠胃黏膜的保护作用研究

本文以50只SPF级SD大鼠为试验对象,设置0.167 g/kg BW低剂量组、0.333 g/kg BW中剂量组、1.000 g/kg BW高剂量组、空白对照组(CK1)、模型对照组(CK2)5个处理,每个处理10只大鼠,其中:低、中、高剂量组分别灌胃200∶1库拉索芦荟凝胶冻干粉,空白对照组(CK1)和模型对照组(CK2)分别灌胃相同体积的蒸馏水。各处理灌胃体积均为10 mL/kg BW,每天1次,连续灌胃30 d。之后,除空白对照组(CK1)外,其他处理以无水乙醇作为急性胃黏膜损伤剂给予大鼠。对大鼠进行解剖,比较各处理大鼠胃黏膜损伤积分指数、胃黏膜病理组织学检查病变总积分,研究200∶1库拉索芦荟凝胶冻干粉对无水乙醇引起的大鼠急性胃黏膜损伤能否产生治疗效果。结果表明:200∶1库拉索芦荟凝胶冻干粉0.167 g/kg BW低剂量组、0.333 g/kg BW中剂量组、1.000 g/kg BW高剂量组对大鼠体质量无明显影响;200∶1库拉索芦荟凝胶冻干粉0.333 g/kg BW中剂量组大鼠胃黏膜损伤积分指数、胃黏膜病理组织学检查病变总积分显著低于模型对照组(CK2)(P<0.05)。可见,200∶1库拉索芦荟凝胶冻干粉对于大鼠胃黏膜有着辅助保护功能。

银杏叶提取物的胃粘膜保护作用

采用大鼠束缚－冷冻应激模型和小鼠无水乙醇损伤模型观察银杏叶提取物（ＧｂＥ）对胃粘膜损伤指数的影响；采用幽门结扎法收集胃液，观察ＧｂＥ对胃液分泌量，胃液酸度和胃蛋白酶活性的影响；采用硫代巴比妥酸（ＴＢＡ）法测定胃粘膜及血清中丙二醛（ＭＤＡ）含量。结果表明，ＧｂＥ（２５，５０，１００ｍｇ／ｋｇ，ｂｉｄ×５ｄ，ｉｇ）可剂量依赖性地抑制束缚－冷冻应激（ＲＣＳ）和无水乙醇引起的胃粘膜损伤。用药组应激后的胃粘膜损伤指数分别为对照组的５８．１１％，４２．９９％和３１．２３％；用药组乙醇诱发的胃粘膜损伤指数降至对照组的６１．５４％，３６．０６％和２５．６４％；ＧｂＥ尚能增强西米替丁对胃粘膜的保护作用。但对大鼠胃液分泌量、胃液酸度及胃蛋白酶活性ＧｂＥ并无明显影响。小鼠经无水乙醇ｉｇ后１ｈ，胃粘膜和血清中的ＭＤＡ含量显著升高（Ｐ＜０．０１），而ＧｂＥ（２５，５０，１００ｍｇ／ｋｇｉｇ）预处理则可以明显抑制ＭＤＡ的升高。因此认为ＧｂＥ具有胃粘膜保护作用，并且与西米替丁在治疗胃溃疡方面具有协同作用，这些作用可能与其抗氧化作用相关。

乙醇性胃粘膜损伤及其保护作用的研究进展

本文主要对乙醇的特点及其在胃肠道中的代谢,乙醇对胃粘膜的损伤作用,对胃粘膜防御因子和自由基的影响,胃粘膜保护机制,以及保护胃粘膜的药物和生物活性物质的研究进展作简要介绍。

鳕鱼皮胶原肽对大鼠胃黏膜损伤的保护作用及其机制

目的:探究不同分子质量段的鳕鱼皮胶原肽对酒精性胃黏膜损伤的保护效果及相关机理。方法:从鳕鱼皮中提取胶原蛋白并用复合酶酶解,制备不同分子质量段的胶原肽,采用高体积分数酒精诱导的大鼠急性胃黏膜损伤模型,观察统计胃黏膜损伤程度并对胃部组织及血清的生理生化指标进行检测。结果;与模型组相比,胶原肽能够减轻大鼠胃腺的出血损伤,降低胃组织中Ca^(2+)含量及髓过氧化物酶活力(P<0.05);其中,中分子质量胶原肽效果最好,该组大鼠胃黏膜损伤指数降至9.21%(P<0.01),损伤抑制率为57.16%;胶原肽能提高胃组织中糖蛋白含量(P<0.05),降低胃组织中丙二醛含量,提高胃组织超氧化物歧化酶活力及血清中谷胱甘肽的含量(P<0,05)。结论:胶原肽能够通过增强大鼠胃黏膜屏障及机体抗自由基氧化水平,产生对急性胃黏膜损伤的保护作用。

心理性应激状态下针刺对大鼠DMV放电、胃电和胃粘膜损伤的影响

目的 探讨针刺对心理性应激状态下大鼠胃动力障碍及胃粘膜损伤的防治作用 .方法 雄性 SD大鼠 96只随机分成 3组 . A:正常对照组 ;B:心理性应激组 ;C:心理性应激+针刺组 .采用高压恒流脉冲刺激器制备心理性应激模型 .电针刺激大鼠双侧足三里 ,细胞外引导测定迷走神经背运动核 (DMV)自发放电 ,浆膜法测定胃电数据及用 Chen等法测定胃粘膜损伤指数 .结果 A组大鼠迷走神经背运动核自发放电较少 ,胃电活动较为规律 ,胃粘膜组织正常 . B组迷走神经背运动核自发放电不规律 ,以放电频率增加占优势 ,胃电活动出现紊乱 ,但与心理性应激的时间和程度无明显相关性 ,胃粘膜可以出现损害、甚至产生溃疡 .且随着受到心理性应激反应程度的不断增强 ,胃粘膜损害的程度不断加大 ,其损伤指数逐渐增加 .但总体上来看仍 B组与 C组相比 ,在心理性应激的 2 ,4,6和 8d二者胃电的频率、波幅都有明显的差异 (P<0 .0 5 ) .相同情况下 ,C组与 B组的胃电差异显著 (P<0 .0 5 ) ;然而 ,C组与 A组相比 ,在心理性应激反应的 2 ,4,6和 8d其胃电的频率和波幅无差异 (P>0 .0 5 ) .而在心理性应激反应后给予电针刺激的条件下 ,胃粘膜损伤明显减轻 ,且随着时间的延长 ,这种效果更加明显 .C组与 B组相比 ,二者的损伤指数有明显差异 (P<0 .

左金丸和反左金对大鼠胃粘膜保护机制的比较研究

【目的】比较左金丸(m_(黄连):m_(吴茱萸)=6:1)和反左金(m_(黄连):m_(吴茱萸)=1:6)对胃分泌和粘液屏障的影响。【方法】采用Shay氏大鼠幽门结扎模型,大鼠分为生理盐水对照组,左金丸1.4g/kg、2.8g/kg组以及反左金1.4g/kg、2.8g/kg组,分别从十二指肠给药,观察各组大鼠的胃分泌和粘液合成的变化。【结果】1.4g/kg、2.8g/kg左金丸和反左金都能抑制胃液分泌、降低总酸排出量及胃蛋白酶活性,且随着剂量增加药效更明显;左金丸和反左金都能增加胃壁结合粘液含量,对胃液中粘液成分也有增加的趋势。【结论】左金丸和反左金可能通过影响胃分泌和粘液合成来保护胃粘膜。