BACKGROUND Pancreatic ductal adenocarcinoma(PDAC)is a common cancer with increasing morbidity and mortality due to changes of social environment.AIM To evaluate the significance of serum carbohydrate antigen 19-9(CA19...BACKGROUND Pancreatic ductal adenocarcinoma(PDAC)is a common cancer with increasing morbidity and mortality due to changes of social environment.AIM To evaluate the significance of serum carbohydrate antigen 19-9(CA19-9)and tumor size changes pre-and post-neoadjuvant therapy(NAT).METHODS This retrospective study was conducted at the Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment,Chongqing University Cancer Hospital.This study specifically assessed CA19-9 levels and tumor size before and after NAT.RESULTS A total of 156 patients who completed NAT and subsequently underwent tumor resection were included in this study.The average age was 65.4±10.6 years and 72(46.2%)patients were female.Before survival analysis,we defined the post-NAT serum CA19-9 level/pre-NAT serum CA19-9 level as the CA19-9 ratio(CR).The patients were divided into three groups:CR<0.5,CR>0.5 and<1 and CR>1.With regard to tumor size measured by both computed tomography and magnetic resonance imaging,we defined the post-NAT tumor size/pre-NAT tumor size as the tumor size ratio(TR).The patients were then divided into three groups:TR<0.5,TR>0.5 and<1 and TR>1.Based on these groups divided according to CR and TR,we performed both overall survival(OS)and disease-free survival(DFS)analyses.Log-rank tests showed that both OS and DFS were significantly different among the groups according to CR and TR(P<0.05).CR and TR after NAT were associated with increased odds of achieving a complete or near-complete pathologic response.Moreover,CR(hazard ratio:1.721,95%CI:1.373-3.762;P=0.006),and TR(hazard ratio:1.435,95%CI:1.275-4.363;P=0.014)were identified as independent factors associated with OS.CONCLUSION This study demonstrated that post-NAT serum CA19-9 level/pre-NAT serum CA19-9 level and post-NAT tumor size/pre-NAT tumor size were independent factors associated with OS in patients with PDAC who received NAT and subsequent surgical resection.展开更多
Seed size is one of the vital traits determining seed appearance, quality, and yield. Untangling the genetic mechanisms regulating soybean 100-seed weight (100-SW), seed length and seed width across environments may p...Seed size is one of the vital traits determining seed appearance, quality, and yield. Untangling the genetic mechanisms regulating soybean 100-seed weight (100-SW), seed length and seed width across environments may provide a theoretical basis for improving seed yield. However, there are few reports related to QTL mapping of 100-SW across multiple ecological regions. In this study, 21 loci associated with seed size traits were identified using a genome-wide association of 5361 single nucleotide polymorphisms (SNPs) across three ecoregions in China, which could explain 8.12%–14.25% of the phenotypic variance respectively. A new locus, named as SW9-1 on chromosome 9 that explained 10.05%–10.93% of the seed weight variance was found significantly related to seed size traits, and was not previously reported. The selection effect analysis showed that SW9-1 locus has a relatively high phenotypic effect (13.67) on 100-SW, with a greater contribution by the accessions with bigger seeds (3.69) than the accessions with small seeds (1.66). Increases in seed weight were accompanied by increases in the frequency of SW9-1T allele, with >90% of the bred varieties with a 100-SW >30 g carrying SW9-1T. Analysis of SW9-1 allelic variation in additional soybean accessions showed that SW9-1T allele accounting for 13.83% of the wild accessions, while in 46.55% and 51.57% of the landraces and bred accessions, respectively, this results indicating that the SW9-1 locus has been subjected to artificial selection during the early stages of soybean breeding, especially the utilization of SW9-1T in edamame for big seed. These results suggest that SW9-1 is a novel and reliable locus associated with seed size traits, and might have an important implication for increasing soybean seed weight in molecular design breeding. Cloning this locus in future may provide new insights into the genetic mechanisms underlying soybean seed size traits.展开更多
核因子-κB受体活化因子配体(receptor activator of Nuclear factor-kappa B Ligand,RANKL)、核因子-κB受体活化因子(receptor activator of nuclear factor-kappa B,RANK)、骨保护素(osteoprotegerin,OPG)作为肿瘤坏死因子家族的成员...核因子-κB受体活化因子配体(receptor activator of Nuclear factor-kappa B Ligand,RANKL)、核因子-κB受体活化因子(receptor activator of nuclear factor-kappa B,RANK)、骨保护素(osteoprotegerin,OPG)作为肿瘤坏死因子家族的成员,通过RANKL/RANK/OPG信号通路调节破骨细胞发生及成熟,调控骨代谢,其表达水平直接影响骨质代谢与重塑,现被广泛研究。RANKL/RANK/OPG通路在口腔颌面部形成及改建过程中发挥了关键作用,直接或间接反映出口腔骨破坏类疾病的发生与发展状态。该通路的失衡往往伴随RANKL的增加和(或)OPG的减少,RANKL/OPG的比值影响破骨细胞的成熟及功能,但部分疾病中相关因子的表达水平存在争议,且尚无能应用于临床疾病诊疗的阈值。本文就RANKL/RANK/OPG信号通路调节机制及其在牙周炎、种植、牙齿发育、正畸、颌面部骨缺损修复与再生等口腔相关领域的研究现状进行综述,以进一步探讨口腔中骨代谢及骨破坏类疾病的调节机制,并评价相关因子作为生物标志物的诊断和预后潜力,以期指导临床诊疗及探寻可能的免疫调节靶点。展开更多
The Al2O3 -SiC-C bricks for iron ladles were pre-pared asing bauxite, fused corundum,pyrophyllite, SiC powder and flake graphite as main starting materials, and phenolic resin as binder. The effect of pyroph,yllite pa...The Al2O3 -SiC-C bricks for iron ladles were pre-pared asing bauxite, fused corundum,pyrophyllite, SiC powder and flake graphite as main starting materials, and phenolic resin as binder. The effect of pyroph,yllite particle size on permanent change in dimensions, cold crushing strength, oxidation resistance, and corrosion resistance of Al2O3 - SiC - C bricks was investigated. The results show that with the decrease of the pyrophyllite particle size, the permanent change in dimensions of Al2O3 - SiC - C bricks decreases, cold crushing strength increases, the oxidation resistance at 1400 ℃ increases, and the corrosion resistance at 1500℃ decreases.展开更多
It has been found that there are marked errors in the value of valid opening size of heat-bonded nonwoven fabrics between theoretical calculations and engineering measurements. A new modified theoretical model is adva...It has been found that there are marked errors in the value of valid opening size of heat-bonded nonwoven fabrics between theoretical calculations and engineering measurements. A new modified theoretical model is advanced in this paper. The equivalent diameter of the pore of a fibre web is used to calculate the valid opening size instead of the maximum diameter of inscribed circle used, because the fibres in practical fibre webs are flexible elastomers with definite diameters and the pore of fibre web may produce deformation in screening teat and engineering usage. The results show that the theoretical calculations coincide well with the engineering measurements. This method offers a theoretical basis for computer simulation to the performance of filters of heatbonded nonwoven fabrics.展开更多
In the current research process of coal rank char gasification reaction in China, it is found that particle size has different influence on the gasification reactivity of coal char of different ranks. Therefore, monod...In the current research process of coal rank char gasification reaction in China, it is found that particle size has different influence on the gasification reactivity of coal char of different ranks. Therefore, monodisperse pulverized coal was prepared from eight kinds of coal chars of different ranks in entrained-flow gasifier. The particle size and gasification temperature of coal char were analyzed for these samples. The degree of influence of carbon dioxide gasification reaction. Through research and analysis, the performance differences of these samples under different carbon conversion rates were compared, and the sample reaction under high carbon conversion rates was discussed. The experimental results show that the orderliness of the microcrystalline structure of coal char is directly proportional to the rank of coal, while the gasification activity of coal char is inversely proportional to the rank of coal. Therefore, for different coal ranks, the influence of coal char particle size on coal char gasification reaction is different. According to the experiments, smaller coal char size and higher gasification temperature can promote the reactivity of higher-order coal gasification. In order to clarify the correlation between particle size and gasification reactivity of coal chars with different ranks, this paper discussed this issue.展开更多
Aims: There has been no evidence on the effects of evolocumab, protein convertase subtilisin/kexin type 9 (PCSK9) inhibitor, on small size LDL. We observationally investigated the efficacy and side effects of evolocum...Aims: There has been no evidence on the effects of evolocumab, protein convertase subtilisin/kexin type 9 (PCSK9) inhibitor, on small size LDL. We observationally investigated the efficacy and side effects of evolocumab on the LDL subfraction particle diameter using PAGE system for lipoprotein analysis. Methods: We defined 30 patients with high-risk hyperlipidemia. As for analysis of LDL subfraction profile, we used polyacrylamide gel electrophoresis three methods: 1) 3% nondenatured poly-acrylamide gel electrophoresis method (3%PAGE), 2) 2% - 16% nondenatured poly-acrylamide gradient gel electro-phoresis method (2% - 16% GGE) and 3) 2.7% - 5% GGE. Evolocumab 140 mg/day administered together with statin significantly improved serum total cholesterol (TC), triglyceride (TG), high-dense lipoprotein-cholesterol (HDL-C), and LDL-C after four-week treatment. Results: TC, TG, HDL-C and LDL-C levels were improved by, respectively, 33%, 20%, 10%, and 54%. The mean LDL size significantly increased from 25.6 ± 0.4 nm to 26.4 ± 0.8 nm. The small dense LDL-cholesterol (sdLDL-C), large buoyant LDL-cholesterol (lbLDL-C), and mid-band lipoprotein-cholesterol were reduced, respectively. Therefore, the preliminary study on this paper can be the first step into a new insight on the world of lipid metabolism. Conclusion: Short-term administration of evolocumab addedons to statin therapy, significantly reduced small size LDL levels.展开更多
基金Natural Science Foundation of Chongqing,China,No.cstc2021jcyj-msxmX0501Chongqing Medical Scientific Research Project(Joint Project of Chongqing Health Commission and Science and Technology Bureau),No.2022QNXM074.
文摘BACKGROUND Pancreatic ductal adenocarcinoma(PDAC)is a common cancer with increasing morbidity and mortality due to changes of social environment.AIM To evaluate the significance of serum carbohydrate antigen 19-9(CA19-9)and tumor size changes pre-and post-neoadjuvant therapy(NAT).METHODS This retrospective study was conducted at the Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment,Chongqing University Cancer Hospital.This study specifically assessed CA19-9 levels and tumor size before and after NAT.RESULTS A total of 156 patients who completed NAT and subsequently underwent tumor resection were included in this study.The average age was 65.4±10.6 years and 72(46.2%)patients were female.Before survival analysis,we defined the post-NAT serum CA19-9 level/pre-NAT serum CA19-9 level as the CA19-9 ratio(CR).The patients were divided into three groups:CR<0.5,CR>0.5 and<1 and CR>1.With regard to tumor size measured by both computed tomography and magnetic resonance imaging,we defined the post-NAT tumor size/pre-NAT tumor size as the tumor size ratio(TR).The patients were then divided into three groups:TR<0.5,TR>0.5 and<1 and TR>1.Based on these groups divided according to CR and TR,we performed both overall survival(OS)and disease-free survival(DFS)analyses.Log-rank tests showed that both OS and DFS were significantly different among the groups according to CR and TR(P<0.05).CR and TR after NAT were associated with increased odds of achieving a complete or near-complete pathologic response.Moreover,CR(hazard ratio:1.721,95%CI:1.373-3.762;P=0.006),and TR(hazard ratio:1.435,95%CI:1.275-4.363;P=0.014)were identified as independent factors associated with OS.CONCLUSION This study demonstrated that post-NAT serum CA19-9 level/pre-NAT serum CA19-9 level and post-NAT tumor size/pre-NAT tumor size were independent factors associated with OS in patients with PDAC who received NAT and subsequent surgical resection.
基金supported by the National Key Research and Development Program of China (2016YFD0100201)the National Natural Science Foundation of China (31771819)+2 种基金the China Postdoctoral Science Foundation (2017M621990)the Introduced Leading Talent Research Team for Universities in Anhui Provincethe Natural Science Foundation of Anhui Province, China (1608085QC66)
文摘Seed size is one of the vital traits determining seed appearance, quality, and yield. Untangling the genetic mechanisms regulating soybean 100-seed weight (100-SW), seed length and seed width across environments may provide a theoretical basis for improving seed yield. However, there are few reports related to QTL mapping of 100-SW across multiple ecological regions. In this study, 21 loci associated with seed size traits were identified using a genome-wide association of 5361 single nucleotide polymorphisms (SNPs) across three ecoregions in China, which could explain 8.12%–14.25% of the phenotypic variance respectively. A new locus, named as SW9-1 on chromosome 9 that explained 10.05%–10.93% of the seed weight variance was found significantly related to seed size traits, and was not previously reported. The selection effect analysis showed that SW9-1 locus has a relatively high phenotypic effect (13.67) on 100-SW, with a greater contribution by the accessions with bigger seeds (3.69) than the accessions with small seeds (1.66). Increases in seed weight were accompanied by increases in the frequency of SW9-1T allele, with >90% of the bred varieties with a 100-SW >30 g carrying SW9-1T. Analysis of SW9-1 allelic variation in additional soybean accessions showed that SW9-1T allele accounting for 13.83% of the wild accessions, while in 46.55% and 51.57% of the landraces and bred accessions, respectively, this results indicating that the SW9-1 locus has been subjected to artificial selection during the early stages of soybean breeding, especially the utilization of SW9-1T in edamame for big seed. These results suggest that SW9-1 is a novel and reliable locus associated with seed size traits, and might have an important implication for increasing soybean seed weight in molecular design breeding. Cloning this locus in future may provide new insights into the genetic mechanisms underlying soybean seed size traits.
文摘核因子-κB受体活化因子配体(receptor activator of Nuclear factor-kappa B Ligand,RANKL)、核因子-κB受体活化因子(receptor activator of nuclear factor-kappa B,RANK)、骨保护素(osteoprotegerin,OPG)作为肿瘤坏死因子家族的成员,通过RANKL/RANK/OPG信号通路调节破骨细胞发生及成熟,调控骨代谢,其表达水平直接影响骨质代谢与重塑,现被广泛研究。RANKL/RANK/OPG通路在口腔颌面部形成及改建过程中发挥了关键作用,直接或间接反映出口腔骨破坏类疾病的发生与发展状态。该通路的失衡往往伴随RANKL的增加和(或)OPG的减少,RANKL/OPG的比值影响破骨细胞的成熟及功能,但部分疾病中相关因子的表达水平存在争议,且尚无能应用于临床疾病诊疗的阈值。本文就RANKL/RANK/OPG信号通路调节机制及其在牙周炎、种植、牙齿发育、正畸、颌面部骨缺损修复与再生等口腔相关领域的研究现状进行综述,以进一步探讨口腔中骨代谢及骨破坏类疾病的调节机制,并评价相关因子作为生物标志物的诊断和预后潜力,以期指导临床诊疗及探寻可能的免疫调节靶点。
文摘The Al2O3 -SiC-C bricks for iron ladles were pre-pared asing bauxite, fused corundum,pyrophyllite, SiC powder and flake graphite as main starting materials, and phenolic resin as binder. The effect of pyroph,yllite particle size on permanent change in dimensions, cold crushing strength, oxidation resistance, and corrosion resistance of Al2O3 - SiC - C bricks was investigated. The results show that with the decrease of the pyrophyllite particle size, the permanent change in dimensions of Al2O3 - SiC - C bricks decreases, cold crushing strength increases, the oxidation resistance at 1400 ℃ increases, and the corrosion resistance at 1500℃ decreases.
文摘It has been found that there are marked errors in the value of valid opening size of heat-bonded nonwoven fabrics between theoretical calculations and engineering measurements. A new modified theoretical model is advanced in this paper. The equivalent diameter of the pore of a fibre web is used to calculate the valid opening size instead of the maximum diameter of inscribed circle used, because the fibres in practical fibre webs are flexible elastomers with definite diameters and the pore of fibre web may produce deformation in screening teat and engineering usage. The results show that the theoretical calculations coincide well with the engineering measurements. This method offers a theoretical basis for computer simulation to the performance of filters of heatbonded nonwoven fabrics.
文摘In the current research process of coal rank char gasification reaction in China, it is found that particle size has different influence on the gasification reactivity of coal char of different ranks. Therefore, monodisperse pulverized coal was prepared from eight kinds of coal chars of different ranks in entrained-flow gasifier. The particle size and gasification temperature of coal char were analyzed for these samples. The degree of influence of carbon dioxide gasification reaction. Through research and analysis, the performance differences of these samples under different carbon conversion rates were compared, and the sample reaction under high carbon conversion rates was discussed. The experimental results show that the orderliness of the microcrystalline structure of coal char is directly proportional to the rank of coal, while the gasification activity of coal char is inversely proportional to the rank of coal. Therefore, for different coal ranks, the influence of coal char particle size on coal char gasification reaction is different. According to the experiments, smaller coal char size and higher gasification temperature can promote the reactivity of higher-order coal gasification. In order to clarify the correlation between particle size and gasification reactivity of coal chars with different ranks, this paper discussed this issue.
文摘Aims: There has been no evidence on the effects of evolocumab, protein convertase subtilisin/kexin type 9 (PCSK9) inhibitor, on small size LDL. We observationally investigated the efficacy and side effects of evolocumab on the LDL subfraction particle diameter using PAGE system for lipoprotein analysis. Methods: We defined 30 patients with high-risk hyperlipidemia. As for analysis of LDL subfraction profile, we used polyacrylamide gel electrophoresis three methods: 1) 3% nondenatured poly-acrylamide gel electrophoresis method (3%PAGE), 2) 2% - 16% nondenatured poly-acrylamide gradient gel electro-phoresis method (2% - 16% GGE) and 3) 2.7% - 5% GGE. Evolocumab 140 mg/day administered together with statin significantly improved serum total cholesterol (TC), triglyceride (TG), high-dense lipoprotein-cholesterol (HDL-C), and LDL-C after four-week treatment. Results: TC, TG, HDL-C and LDL-C levels were improved by, respectively, 33%, 20%, 10%, and 54%. The mean LDL size significantly increased from 25.6 ± 0.4 nm to 26.4 ± 0.8 nm. The small dense LDL-cholesterol (sdLDL-C), large buoyant LDL-cholesterol (lbLDL-C), and mid-band lipoprotein-cholesterol were reduced, respectively. Therefore, the preliminary study on this paper can be the first step into a new insight on the world of lipid metabolism. Conclusion: Short-term administration of evolocumab addedons to statin therapy, significantly reduced small size LDL levels.
