Study on Absorption and Regeneration Performance of Novel Hybrid Solutions for CO_2 Capture

Recently, a kind of hybrid solution MEA-methanol shows a better CO_2 capture performance over aqueous MEA solution. However, the vaporization of methanol is the biggest disadvantage that hinders its application, so it is necessary to minimize the vaporization of methanol during both the absorption and regeneration processes. In this work, two kinds of hybrid solutions were studied and compared with aqueous MEA solution and MEA-methanol solution, including MEA/TEA/methanol solution and MEA/glycerol/methanol solution. The absorption property of MEA/glycerol/methanol solution is better than aqueous MEA solution within a certain period of time and the absorption property of MEA/TEA/methanol solution is too poor to be used in CO_2 capture. By increasing the concentration of TEA and decreasing the concentration of MEA, the absorption rate, CO_2 capture efficiency and absorption capacity all decreased. Upon adding glycerol, the cyclic capacity decreased and the generation temperature increased, and moreover, the density and viscosity also increased considerably. So after adding TEA and glycerol, the CO_2 capture performance of MEAmethanol solvent cannot be improved.

Identification of differentially expressed genes of primary spermatocyte against round spermatid isolated from human testis using the laser capture microdissection technique

The method of laser capture microdissection (LCM) combined with suppressive subtractive hybridization (SSH) was developed to isolate specific germ cells from human testis sections and to identify the genes expressed during differentiation and development. In the present study, over 10,000 primary spermatocytes and round spermatid cells were successfully isolated by LCM. Using the cDNAs from primary spermatocytes and round spermatids, SSH cDNAs library of primary spermatocyte-specific was constructed. The average insert size of the cDNA isolated from 75 randomly picked white clones was 500 bp, ranging from 250 bp to 1.7 kb. Using the dot-blot method, a total of 421 clones were examined, resulting in the identification of 390 positive clones emitting strong signals. Partial sequence of cDNAs prepared from each clone was determined with an overall success rate of 84.4%. Genes encoding cytochrome c oxidase II and the rescue factor-humanin were most frequently expressed in primary spermatocytes, suggesting their roles involved in meiosis.

Gradient-augmented hybrid interface capturing method for incompressible two-phase flow

Motivated by inconveniences of present hybrid methods,a gradient-augmented hybrid interface capturing method（GAHM） is presented for incompressible two-phase flow.A front tracking method（FTM） is used as the skeleton of the GAHM for low mass loss and resources.Smooth eulerian level set values are calculated from the FTM interface,and are used for a local interface reconstruction.The reconstruction avoids marker particle redistribution and enables an automatic treatment of interfacial topology change.The cubic Hermit interpolation is employed in all steps of the GAHM to capture subgrid structures within a single spacial cell.The performance of the GAHM is carefully evaluated in a benchmark test.Results show significant improvements of mass loss,clear subgrid structures,highly accurate derivatives（normals and curvatures） and low cost.The GAHM is further coupled with an incompressible multiphase flow solver,Super CE/SE,for more complex and practical applications.The updated solver is evaluated through comparison with an early droplet research.

基于改进混合粒子群优化算法的多无人机协同围捕方法研究

针对多无人机协同围捕问题,在无人机运动学约束基础上,考虑各无人机应同时到达围捕位置,提出了多机协同围捕任务规划两层求解架构。在任务协调层通过改进混合粒子群优化方法,以各无人机同时到达指定围捕位置的最小时间为目标,优化调度给出多目标围捕方案;在航路规划层考虑无人机初始状态及运动学约束,通过Dubins曲线调整实现各无人机同时到达围捕位置。仿真结果表明了所提方法的有效性。

基于捕获点理论的混合驱动水下刀锋腿机器人稳定性判据

由8个推进器和6条刀锋腿混合驱动的水下机器人可在水底或水下结构物表面上行走.所提方法旨在研究这类机器人运动稳定性的评判准则,即稳定性判据.现有的稳定性判据多集中于同一机构(腿)驱动的陆地机器人,未涉及混合驱动的水下刀锋腿机器人.针对该问题,提出了基于捕获点理论的混合驱动水下刀锋腿机器人稳定性判据.首先,在建立混合驱动水下滚动倒立摆模型的基础上,利用机器人运动状态预测摆动腿和支撑腿切换瞬间机器人的动能;然后,根据推进器所能提供的推力范围,计算迫使机器人静止的捕获点变化范围,即获取捕获域;最后,根据捕获域与支撑域的空间关系,判断机器人是否稳定,并计算机器人的稳定裕度.水下实验结果表明,所提出的稳定性判据具有较好的充要性和普适性.

HPV诊断中应用PCR检验的效果

目的分析高危型人乳头瘤病毒(HPV)诊断中应用实时聚合酶链式反应(PCR)检验的效果。方法以该院2023年1-12月内收治的101例高危型HPV患者为本次研究对象,所有研究对象先使用第二代杂交式捕获法(HC-Ⅱ)检验,再行实时PCR检验,以病理实验检测结果为金标准,统计对比两种检验方式的诊断效能。结果组间对比,HC-Ⅱ与实时PCR对高危型HPV的诊断特异度差异无统计学意义(P>0.05),但实时PCR对高危型HPV的诊断准确率、灵敏度均显著高于HC-Ⅱ,差异有统计学意义(P<0.05)。结论实时PCR对于高危型HPV感染患者具有较高检出率,可为此类患者的早期治疗提供科学依据,倡导临床应用。

Coassembled ionic liquid/laponite hybrids as effective CO2 adsorbents

Hybrid adsorbents for COcapture were prepared by coassembling laponite（LP） nanosheets and 1-nbutyl-3-methylimidazolium chloride（BMIMCl）. The prepared BMIMCl/LP layered hybrids were systematically characterized. The interlayer distance of the BMIMCl/LP layered hybrids expanded with an increasing concentration of BMIMCl, indicating that cumulative BMIMCl was intercalated into the LP layers. The efficiency of BMIMCl toward COcapture was significantly enhanced after it was immobilized within LP layers.

Hybrid Passive/Active Vibration Control of a Loosely Connected Spacecraft System

In this paper,a hybrid passive/active vibration(HPAV)controller of a loosely connected spacecraft consisting of a servicing satellite,a target and an X-shape structure isolator is first proposed to suppress vibrations of the system when subjected to the impulsive external excitations during the on-orbit missions.The passive dynamic response of the combined system can be adjusted appropriately to achieve the desired vibration isolation performance by tuning the structural parameters of the bio-inspired X-shape structure.Moreover,the adaptive control design through dynamic scaling technique is selected as the active component to maintain high vibration isolation performance in the presence of parameter uncertainties such as mass of the satellite platform,the damping and rotation friction coefficients of the X-shape structure.Compared with the pure passive system and the traditional spring-mass-damper(SMD)isolator,the HPAV strategy witnesses lower transmissibility,smaller vibration amplitude and higher convergence rate when subjected to the post-capture impact.Numerical simulations demonstrate the feasibility and validity of the proposed hybrid control scheme in suppressing vibrations of the free-floating spacecraft.

Solar radiation-use characteristics of indica/japonica hybrid rice (Oryza sativa L.) in the late season in southeast China

New indica and japonica hybrid rice cultivars,such as the Yongyou series,provide farmers with very high yield potential.However,information on their canopy light capture and solar radiation use efficiency in the late season is limited.Field experiments were performed to compare the radiation-use parameters of four rice types:indica rice(IR),inbred japonica rice(IJR),hybrid japonica rice(HJR),and hybrid indica/japonica rice(HIJR),from 2016 to 2018 during the late season in Hangzhou,China.The grain yield,aboveground biomass,intercepted solar radiation(SI),and radiation-use efficiency(RUE)of the HIJR were on average respectively 13.4%–53.4%,14.3%–30.6%,7.6%–21.4%,and 8.2%–14.9%higher than those of the HJR,IJR,and IR.The leaf area index(LAI)of the HIJR was 18.2%–57.0%greater than that of the IJR and HJR at four growth stages,resulting in respectively 17.8%–38.5%and 10.7%–42.8%greater canopy light interception rates(LIR)and amount of intercepted solar radiation during the vegetative stage.The prolonged grain-filling stage also led to respectively 33.9%–52.6%and 30.5%–51.4%increases in amounts of incident and intercepted radiation for the HIJR relative to the IR during grain filling.These results indicate that the SI superiority of the HIJR was caused by canopy closure as rapid as that of the IR during the vegetative stage(greater LAI and canopy LIR during the growing season)and a grain-filling stage as long as that of the HJR.For grain-filling stage,differences in leaf Pn between HIJR,IR,and IJR were not significant,suggesting that the greater RUE of the HIJR(12.7%–52.8%higher)than that of the other rice types resulted from improved canopy architecture after flowering(FL).Principal components analysis(PCA)revealed that the superiority of the HIJR in terms of solar radiation use resulted from the greater canopy light capture capability of IR and the prolonged growth period(especially during grain filling)of japonica rice in the late growing season.

凹空细胞检测在宫颈癌前病变筛查中的应用及其与Hybrid Capture 2-HPV检测方法的比较

宫颈癌是发展中国家最常见的女性生殖系统恶性肿瘤,在发达国家,其发病率仅次于乳腺.高危型人乳头瘤病毒（HPV）感染与宫颈癌的关系已得到流行病学、分子生物学、临床研究的证实,其中以HPV16型所占比例最高.

高危型HPV病毒载量在不同年龄段宫颈癌筛查中的应用研究

目的探究高危型人类乳头瘤病毒(HPV)病毒载量在不同年龄段宫颈癌筛查中的不同意义。方法回顾性分析2016年~2020年在唐山市妇幼保健院妇科门诊进行宫颈癌筛查HPV病毒阳性的女性患者1338例,采用二代杂交捕获试验(HC2)进行高危型HPV-DNA检测,并于阴道镜下宫颈多点活检,病毒载量由荧光度数与阴性测定值比值(RLU/CO)来衡量,从组织病理活检结果得到宫颈病变程度。探讨病毒载量在不同年龄段患者,尤其是低年龄与高年龄段中与宫颈病变程度的关系。结果30岁以下组及50岁以上组的病毒载量与30~50岁组比较,差异有统计学意义(P<0.05)。30岁以下HPV阳性患者组中低病毒载量(<100 RLU/CO)比例较高,达74.1%,30~50岁组与50岁以上组中均为高病毒载量比例较高,分别是64.4%和54.7%。30岁以下并携带高病毒载量的患者宫颈病变程度相对更高(P<0.05),而50岁以上的患者携带低病毒载量宫颈病变程度更高(P<0.05)。结论在30岁以下及50岁以上年龄段中,相同病毒载量在宫颈癌筛查工作中有着不同意义,需与30~50岁中年患者的诊治工作做出区分,为未来不同年龄段HPV感染患者的宫颈病变程度进行针对性诊治提供理论依据。

古建筑木结构用材树种的DNA分子鉴定方法研究

古建筑木结构是自然生态与人类文明的重要载体。开展古建筑木构件树种鉴定研究,是古建筑木结构维修和保护的重要基础,同时也能加深了解古代先民掌握与利用木材资源的方式,进一步提高我们对古代人类文明的理解和认知。本研究以我国明十三陵和太庙古建筑的“楠木”构件为研究对象,通过构建包括古DNA提取、液相杂交捕获及数据分析在内的木材古DNA分子鉴定方法,实现了从古建筑木构件样品中获得高质量质体DNA基因组,覆盖度达到99.04%~99.94%,平均测序深度为17~110×;所获取DNA片段平均长度为90~138 bp,片段末端发生碱基替换,符合古DNA的典型特征;木构件样品树种鉴定为楠木(Phoebe zhennan)和细叶楠(P.hui)。本研究构建的古DNA分子鉴定方法将为古建筑木结构用材树种精准鉴定提供科技支撑。

纳米有机杂化材料(NOHMs)用于CO_(2)捕集的研究进展

近年来,纳米有机杂化材料(NOHMs)引起了广泛的研究。不同于捕集CO_(2)的传统胺基溶剂,NOHMs可以在不含任何溶剂的条件下保持类液性,具有可忽略蒸汽压和高热稳定性的特点,因此可以减少溶剂挥发带来的损失以及环保问题,同时减少了对设备的腐蚀,是一种有潜力的替代传统胺基溶剂的吸收剂。同时NOHMs可直接作为吸附剂吸附CO_(2),也可作为添加剂和其他材料产生协同作用,比如作为分离膜中的掺杂剂加入到聚合物基质中制备混合基质膜,可以显著强化CO_(2)在膜内的传质速率,实现CO_(2)的高效捕集。本文概括了NOHMs在碳捕集领域的研究进展,分别针对共价键和离子键连接的两类NOHMs阐述其核心、内冠、外冠三部分对CO_(2)吸附性能的影响,探讨和归纳了核心的尺寸、孔隙率、含量以及冠层的密度、链长和结构等对NOHMs碳捕集能力的影响机制,总结了以上因素与焓效应和熵效应的对应关系,以及对碳捕集性能的作用。本文将为拓宽NOHMs在CO_(2)捕集领域的应用提供参考与新的研究思路。

Programmable DNA-responsive microchip for the capture and release of circulating tumor cells by nucleic acid hybridization

The detection and analysis of circulating tumor cells （CTCs） from patients＇ blood is important to assess tumor status; however, it remains a challenge. In the present study, we developed a programmable DNA-responsive microchip for the highly efficient capture and nondestructive release of CTCs via nucleic acid hybridization. Transparent and patternable substrates with hierarchical architectures were integrated into the microchip with herringbone grooves, resulting in greatly enhanced cell-surface interaction via herringbone micromixers, more binding sites, and better matched topographical interactions. In combination with a high-affinity aptamer, target cancer cells were specifically and efficiently captured on the chip. Captured cancer cells were gently released from the chip under physiological conditions using toehold-mediated strand displacement, without any destructive factors for cells or substrates. More importantly, aptamercontaining DNA sequences on the surface of the retrieved cancer cells could be further amplified by polymerase chain reaction （PCR）, facilitating the detection of cell surface biomarkers and characterization of the CTCs. Furthermore, this system was extensively applied to the capture and release of CTCs from patients＇ blood samples, demonstrating a promising high-performance platform for CTC enrichment, release, and characterization.

实时荧光PCR技术在高危型人乳头瘤病毒检测中的价值

目的 分析实时荧光PCR技术在高危型人乳头瘤病毒(HPV)检测中的价值。方法 选取本院行病理诊断检查的66例高危型HPV感染者及68例健康女性为研究对象,均实施实时荧光PCR技术和第二代杂交捕获试验(HC-Ⅱ)技术行HPV检测,比较两种检测技术的诊断效能。结果 以病理检验结果为金标准,实时荧光PCR技术的准确度、灵敏度、特异度、阳性预测值、阴性预测值、诊断符合率分别为94.03%、96.97%、91.18%、91.43%、96.88%、94.03%,高于HC-Ⅱ技术的76.12%、81.82%、70.59%、72.97%、80.00%、76.12%,差异有统计学意义(P<0.05)。结论 高危型HPV检测中应用实时荧光PCR技术,能够保证疾病检测结果的准确性和可靠性,进而为后续的临床治疗提供支持,应用价值较高。

4种不同检查方法在宫颈癌筛查中的临床应用价值

目的：了解4种不同检查方法在宫颈癌筛查中的临床应用价值。方法：采用第二代杂交捕获技术（hyhrid capture 2，Hc—Ⅱ）检测13种高危型人乳头瘤病毒（human papillomavirus，HPV）、薄层液基细胞学技术（Liquid—hased cytologytest，LCT）检测宫颈脱落细胞、醋酸肉眼检查（visual inspection with acetic acid，VIA）和阴道镜检查4种方法对2004年11月～2004年12月深圳南山区华侨城区域15～59岁有性生活女性共1137例进行盲法同步宫颈癌筛查。对阴道镜检查异常或可疑异常者行阴道镜下直接活检；对HPV阳性并且LCT≥未明确诊断意义的不典型鳞状上皮细胞（atypical squamous cells of undetetemined sign，Ascus），或HPV阴性但LCT≥低度鳞状上皮内病变（low grade squamous intraepithelial lesion，LSIL）的妇女再次行阴道镜下活组织病理学检查，以病理结果作为验证4种检查方法的金标准。结果：共取病理122例。病理结果证实该人群中无宫颈癌病例：子宫颈上皮内瘤变（cervical intraepithlial neoplasia。CIN）Ⅲ级3例，CINⅡ级11例，CINⅠ级36例；慢性宫颈炎和鳞状上皮化生69例；正常宫颈3例。人群的高危HPV总检出率为14．0％；LCT阳性率为12．6％；VIA阳性率为12．5％；阴道镜阳性率为13，6％。随宫颈病变级别升高，高危HPV感染率及LCT阳性率均呈趋势性增加（P〈0．005）；VIA和阴道镜阳性率在各级宫颈病变中无统计学差异，但在宫颈病变组阳性检出率明显高于正常宫颈组。高危HPV对宫颈高度病变的敏感性、特异性、准确性、阳性预测值、阴性预测值、阳性似然比和阴性似然比分别为100％、87．1％、87.3％、8．8％、100％、7．6％和0；LCT以上各指标分别为92．9％、88．4％、88．5％、9．1％、99．9％、8．0％和8．0％；VIA以上各指标分别为35．7％、96．0％、95．3％、10．0％、99．2％、8．9％和67．0％；阴道镜以上各指标分别为50．0％、86．8％、86．4％、4．5％、99．3％、3．8％和58．0％。结论：高危HPV检测和LCT检查均为目前宫颈癌筛查较好的方法，VIA和阴道镜检查敏感性较差，漏诊率高，不适合大范围筛查，但二者阴性预测值均较高，可应用于临床病例诊断。

高危型HPV负荷量与宫颈癌及其前期病变关系的研究

目的:探讨高危型HPV负荷量与宫颈癌及其前期病变的关系。方法:对2005年1月至2006年12月于本院行宫颈癌筛查的1221例患者临床资料进行统计分析,观察高危型HPV负荷量和宫颈癌及其前期病变的关系,并用ROC曲线分析,确定HC-Ⅱ法检测高危型HPV-DNA判断宫颈病变≥CINⅡ理想的RLU/CO界值。结果:1221例患者组织学诊断为慢性宫颈粘膜炎667例,CIN407例(其中CINⅠ109例、CINⅡ～Ⅲ298例),宫颈癌147例。慢性宫颈炎、CINⅠ、CINⅡ～Ⅲ和宫颈癌患者高危HPV-DNA负荷量的中位数分别为32.58,58.16,103.83和173.68。根据ROC曲线,统计结果中各可能切点的灵敏度和特异度,发现确定预测≥CINⅡ宫颈病变最佳RLU/CO值为3.155,该点灵敏度88%,特异度57%,Youden指数0.446。结论:高危型HPV负荷量与宫颈癌及其前期病变存在明显相关性,预测≥CINⅡ宫颈病变高危HPV负荷量最佳值为3.155。

高危型人乳头瘤病毒杂交捕获法Ⅱ评估宫颈上皮内瘤变的临床价值

目的探讨高危型人乳头瘤病毒杂交捕获法Ⅱ(HC2)评估宫颈上皮内瘤变的临床价值。方法回顾性分析了2002年3月至今在我院妇科门诊进行宫颈液基薄层细胞学(TCT)检测和HC2二联筛查的723例患者,以及其中进行TCT、HC2和阴道镜下多点活检三联检查的350例患者的临床资料。结果在TCT和HC2二联筛查的723例患者中,不典型鳞状细胞(ASC)组、低度鳞状上皮内病变(LSIL)组和高度鳞状上皮内病变(HSIL)组高危型HPV感染率分别为70·7%(94/133)、88·9%(249/280)和90·9%(90/99),均明显高于TCT正常组的55·5%(117/211)(P=0·005,P<0·001,P<0·001)。在TCT、HC2和阴道镜加点活检三联检查的350例患者中,宫颈轻度不典型增生(CIN1)组和宫颈中度不典型增生(CIN2)组高危型HPV感染率分别为88·9%(72/81)和96·3%(52/54),均明显高于病理学结果正常组的77·7%(153/197)(P=0·03,P=0·002);宫颈重度不典型增生(CIN3)组和子宫颈癌组高危型HPV感染率分别为91·7%(11/12)和100·0%(6/6),也呈明显升高趋势。在三联检查TCT为ASC的患者群中,高危型HPV感染率为79·3%(69/87)。其中高危型HPV感染者发生CIN2-3的比率为38·0%,明显高于无高危型HPV感染者的5·9%(P=0·04)。结论高危型HPV感染与宫颈癌前病变及子宫颈癌密切相关,其感染率随宫颈病变严重程度呈上升趋势。

210例高危型人乳头瘤病毒感染检测及宫颈病变影响因素的探讨

目的：探讨高危型人乳头瘤病毒（HPV）检测及影响其感染妇女宫颈病变程度的因素。方法：选择我院2011年7～10月行第二代基因杂交捕获技术（HC2）检测高危型HPVDNA阳性的210例患者临床资料进行分析。210例患者同时期行TCT检查及阴道镜病理活检。将210例患者的年龄和病毒负荷（RLU/CO比值）作为自变量，宫颈病变程度作为应变量，采用多元线性回归分析，了解高危型HPV感染患者的年龄和病毒负荷与宫颈病变的严重程度是否有相关性。结果：@210例HC2阳性妇女中，随着宫颈病变程度的加重，TCT的阳性率明显升高，CIN组（71．05％）和宫颈癌组（60．00％）显著高于宫颈炎组（13．33％）和尖锐湿疣组（28．21％），差异有统计学意义（P〈0．05）。但TCT的假阴性率也较高。@210例高危型HPV感染患者的年龄和病毒负荷与宫颈病变的严重程度有显著相关性（P〈0．05），病毒负荷越高、年龄越大，发生严重宫颈病变的风险越高。结论：高危型HPV感染患者的年龄及病毒负荷与宫颈病变的风险密切相关。对于HC2阳性的患者，即使TCT结果阴性，也可考虑行阴道镜检查。

HPV感染种系型别与宫颈癌变的相关性研究

目的研究妇女下生殖道人乳头瘤病毒(HPV)感染的种系型别分布,探讨不同基因型HPV与子宫颈癌变的相关性。方法对我院门诊476例宫颈HPV感染的患者以第二代杂交捕获(HC-Ⅱ)试验检测13种高危型HPV,同时采用核酸分子快速导流杂交基因分型技术(HybriMax)进行21种HPV-DNA亚型分析,分析HPV感染型别与宫颈癌变的相关性。结果 476例患者中,HybriMax法检测13种高危型HPV的阳性率为84.9%,HC-Ⅱ法为85.7%,两种方法的总符合率为95.8%,Kappa指数(KI)为0.83。HPV亚型感染频度由高到低依次为16、58、52、11、33、18、68、31、6、39、53、66、CP8304、51、56、45、59、44和42型,宫颈癌/HSIL组最常见的前6位型别由高到低为16(16.4%)、58(11.3%)、52(8.0%)、33(3.8%)、18(3.4%)和31(2.5%),LSIL组为16(8.4%)、58(4.6%)、18(3.4%)、33(3.4%)、39(2.9%)和68(2.9%),正常组织/炎症组为16(5.5%)、11(5.0%)、58(4.2%)、6(2.9%)、31(2.1%)和10(2.1%)。正常组织/炎症组、LSIL组、宫颈癌/HSIL组中两种以上HPV亚型感染者分别占22.7%、33.7%和55.3%,最后一组明显高于前两组,差异有统计学意义(P<0.05)。结论哈尔滨地区妇女下生殖道感染HPV型别以16、58、52、11、33、18型较为多见,其中16、58、52、33和18型的致癌性较强。HPV-DNA分型检测在宫颈癌变的临床诊断和预后判断方面具有重要意义。