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Mutagenic Effects of Spaceflight Treatment on the Second-Generation Seeds of Alfalfa
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作者 WANG Mi REN Wei-bo +2 位作者 GUO Hui-qin XU Zhu CHEN Li-bo 《Animal Husbandry and Feed Science》 CAS 2010年第8期47-48,共2页
[ Objective] To explore the effects of spaceflight on the second-generation seeds of alfalfa and provide a theoretical basis for mutation breeding. [Method] The seeds of Medicago stavia L. lines no. 1, no. 2 and no. 4... [ Objective] To explore the effects of spaceflight on the second-generation seeds of alfalfa and provide a theoretical basis for mutation breeding. [Method] The seeds of Medicago stavia L. lines no. 1, no. 2 and no. 4 were carried into space by the Shijian-8 seed breeding satellite for a 15-d spaceflight treatment. After returning to the ground, seedlings were transplanted to field. Traits of the second-generation seeds of alfalfa were evaluated. [Result] The 1 000-grain weight of the second-generation seeds were 5% -9% significantly higher than that the control (P 〈 0.05). The germination rate, seedling weight, shoot length and root length were significantly increased (P 〈 0.05). The hard seed rate and the rate of moldy seeds were significantly decreased ( P 〈 0.05). However, the rate of dead seeds was increased. [ Conclusion] Spaceflight treatment has positive mutagenic effects on the second-generation seeds of alfalfa. 展开更多
关键词 SPACEFLIGHT Medicago sativa second-generation seeds
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Branching and growth of plantings in fifth year of a seedling seed orchard of Masson pine (Pinus massoniana Lamb.) 被引量:3
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作者 赖焕林 王章荣 江瑞荣 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第1期28-32,83,共5页
The features of branching and growth studied included height, diameter at breast height (DBH), total number of branches, annual height growth, annual branch elongation in the year of elongating, annual branch number f... The features of branching and growth studied included height, diameter at breast height (DBH), total number of branches, annual height growth, annual branch elongation in the year of elongating, annual branch number for four consecutive years, diameter of branches of different ages, and diameter of stem where branch-whorl originates. For features of total growth and overall branching, no significant differences were found between families, except for DBH. For annual features, no significant differences were found in annual stem height growth, annual branch elongation in the year of elongation and diameter of branches. In the last four years, differences in number of branches were not significant in the first two years but were significant in the last two year; differences in stem diameter where branch-whorls grow were significant for the four consecutive years. Trend of annual growth and branching features of families can be divided into three types as increasing type, stable type and fluctuating type. Most of families have an increasing type with respect of annual height growth and annual branch elongation, while most families belong to a fluctuating type with annual branch number. The results indicated that in the fifth year after planted in seedling seed orchard, differences between families were mostly insignificant. This result may have two main explanations: one is the growth rhyme in early ages of Masson pine, the other one is the complex paternal components to form the open-pollinated families. Family selection seemed to be not useful based on the result. It is suggested to select some of families in the nursery instead of to use all the families when establishing seedling seed orchards with open-pollinated families from plus-trees. 展开更多
关键词 Pinus massoniana Lamb. Branching GROWTH seedling seed orchard
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Phenological observations on Larix principis-rupprechtii Mayr. in primary seed orchard 被引量:5
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作者 张新波 任建茹 张旦儿 《Journal of Forestry Research》 SCIE CAS CSCD 2001年第3期201-204,210,共4页
Through 5 years of phenological observations on Larix principis-rupprechtii Mayr. in primary seed orchard and studies on population and individuals of clones, the annual periodic phenological laws were revealed and th... Through 5 years of phenological observations on Larix principis-rupprechtii Mayr. in primary seed orchard and studies on population and individuals of clones, the annual periodic phenological laws were revealed and the annual phe-nological periodic table was drawn up. The correlation between various phenophases, the air temperature and active accumu-lated temperature were analyzed and expounded. The authors also analyzed the similarities and differences of phenophases among clonal individuals as well as the blooming properties of male and female flowers at the same time. This study could pro-vide theoretical reference for working out the production plan of improved varieties and other management measures in seed orchard of Larix principis-rupprechtii. 展开更多
关键词 Larix principis-rupprechtii Mayr. Primary seed orchard Phenological observation
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Evaluation of seed production in a first-generation seed orchard of Chinese pine (Pinus tabuliformis) 被引量:4
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作者 Huwei Yuan Shihui Niu +3 位作者 Xianqing Zhou Qingping Du Yue Li Wei Li 《Journal of Forestry Research》 SCIE CAS CSCD 2016年第5期1003-1008,共6页
We evaluated seed production in a first-gener- ation orchard of Chinese pine (Pinus tabuliformis) during the crucial transition period from first generation to advanced generations. Clones varied significantly in al... We evaluated seed production in a first-gener- ation orchard of Chinese pine (Pinus tabuliformis) during the crucial transition period from first generation to advanced generations. Clones varied significantly in all traits related to seed production. Repeatability of these traits ranged from 0 to 0.96. Seed production per ramet (SPPR), seed producing index (SPI), the number of relative female strobili (RFS), the number of scales, and the num- ber of ineffective scales had comparatively high repeata- bility at 0.86, 0.87, 0.89, 0.96, and 0.91, respectively. Correlation analysis showed that SPPR was greatly influenced by RFS and by the number of full seeds per cone. Finally, we showed that SPI was the best predictor of the seed-producing ability of clones. Our findings will assist seed orchard managers in effectively predicting and improving seed production of Chinese pine seed orchards. 展开更多
关键词 Chinese pine tabuliformis. seed orchard Cone HERITABILITY PINUS seed production
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Mode of pollen spread in clonal seed orchard of Pinus koraiensis 被引量:4
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作者 Fu-Juan Feng Xin Sui +3 位作者 Min-Min Chen Dan Zhao Hi-Jie Han Mai-He Li 《Journal of Biophysical Chemistry》 2010年第1期33-39,共7页
The patterns of effective pollen dispersal and the relationships between pollen dispersal and genetic composition in Pinus koraiensis are still unclear. Hence, we investigated the mode of pollen dispersal of P. koraie... The patterns of effective pollen dispersal and the relationships between pollen dispersal and genetic composition in Pinus koraiensis are still unclear. Hence, we investigated the mode of pollen dispersal of P. koraiensis in a clonal seed orchard in Lushuihe Forestry Bureau, Jilin Province, using SSR molecular markers tech-nique and the method of maximum likelihood. A total of 13 pairs of nuclear microsatellites po-lymorphic primers were used in the paternity analysis. We analyzed 100 progenies and 150 paternities. A total of 56 alleles were detected in 13 loci with 3-6 alleles (4.3 alleles in average) in a single locus. The primers of SsrPt_ctg7170 and SsrPt_ctg5333 had the maximum (6) and the minimum (3) alleles, respectively. The averaged values of observed heterozygosity, expected heterozygosity and polymorphism information content of experimental groups were 0.648, 0.601 and 0.533, respectively. The mating dis-tance of P. koraiensis followed an approximately normal distribution. The most effective pollen for the female parent came from male parent trees 15-45 m away with an average mating dis-tance of 32.60 m and the longest of 67.88 m. The pollen source of offspring was not random but greatly affected by the wind direction during the pollination season of P. koraiensis. These re-sults have important implications for seed or-chard design to improve the genetic quality of seeds and seed production. 展开更多
关键词 PINUS Koraiensis seed orchard Nuclear Microsatellites MARKERS PATERNITY Analysis POLLEN SPREAD
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Improved genetic distance-based spatial deployment can effectively minimize inbreeding in seed orchard 被引量:1
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作者 Boning Yang Haihong Sun +3 位作者 Jiandong Qi Shihui Niu Yousry AEl-Kassaby Wei Li 《Forest Ecosystems》 SCIE CSCD 2020年第1期117-124,126,125,127,共11页
Background:Inbreeding in seed orchards is expected to increase with the advancement of breeding cycles,which results in the delivery of crops with suboptimal genetic gain,reduced genetic diversity,and lower seed set.H... Background:Inbreeding in seed orchards is expected to increase with the advancement of breeding cycles,which results in the delivery of crops with suboptimal genetic gain,reduced genetic diversity,and lower seed set.Here,a genetic distance-dependent method for clonal spatial deployment in seed orchards was developed and demonstrated,which reduced the inbreeding levels.The method’s main evaluation parameter of inbreeding is the genetic distance among individuals and the deployment method used an improved adaptive parallel genetic algorithm(IAPGA)based on Python language.Using inbreeding-prone Chinese Mongolian pine breeding population material originating from a single natural population,the proposed method was compared to a traditional orchard design and a distance-based design;namely,complete randomized block(RCB)and optimum neighborhood(ONA)designs,respectively.Results:With the advancement of selective breeding cycles,group separation among orchard related individuals is expected to increase.Based on the genetic distance among individuals,the IAPGA design was superior in significantly reducing the inbreeding level as compared to the two existing designs,confirming its suitability to advanced-generation orchards where relatedness among parents is common.In the 1st,2nd,and mixed generations clonal deployment schemes,the IAPGA design produced lower inbreeding with 87.22%,81.49%,and 87.23%of RCB,and 92.78%,91.30%,and 91.67%of ONA designs,respectively.Conclusions:The IAPGA clonal deployment proposed in this study has the obvious advantage of controlling inbreeding,and it is expected to be used in clonal deployment in seed orchards on a large-scale.Further studies are needed to focus on the actual states of pollen dispersal and mating in seed orchards,and more assumptions should be taken into account for the optimized deployment method. 展开更多
关键词 seed orchard DEPLOYMENT INBREEDING GENETIC distance CLONE SSR
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Options for converting a clone trial of Eucalyptus camaldulensis into a clonal seed orchard considering gain,fertility and effective clone number
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作者 R.Kamalakannan M.Varghese +1 位作者 P.G.Suraj T.Arutselvan 《Journal of Forestry Research》 SCIE CAS CSCD 2016年第1期51-57,共7页
We evaluated a clone trial comprised of 20 ramets each of 40 Eucalyptus camaldulensis clones for growth and fertility at 4 years of age. The clones differed significantly in growth with 65-100 % survival in 38 clones ... We evaluated a clone trial comprised of 20 ramets each of 40 Eucalyptus camaldulensis clones for growth and fertility at 4 years of age. The clones differed significantly in growth with 65-100 % survival in 38 clones while two clones had low survival (40 and 10 %). Fecundity was high and fertility variation low, as indicated by the sibling coefficient value (ψ = 1.4) with 55 % of clones contributing 80 % of the fruits. Thinning strategies were considered to convert the clone trial to a clonal seed orchard and enhance both gain and diversity in seed crop. When clones were selected based on growth without con- sidering fertility, truncation selection (to retain clones with higher DBH than the trial mean value) would give 8 % gain, but 59 % reduction in effective population size (Ne). Gain and predicted diversity (Ne) was highest when ramets were selected in linear proportion to the breeding value of each clone. Mass selection with the same intercept (for DBH) as truncation selection would give a moderate 3 % gain, but over two times higher predicted Ne than trunca- tion selection. When fertility of retained trees was con- sidered after thinning, mass selection would yield 52 % effective contribution from the orchard trees compared to only 30 % contribution from truncation selection and linear thinning. Higher representation of superior clones in linear thinning and mass selection would lead to greater fertilityvariation and over 30 % reduction in effective clone number (Nc) from that predicted (Ne, assuming equal fer- tility among ramets) before thinning. 展开更多
关键词 seed orchard Fertility variation Geneticgain Diversity THINNING EUCALYPTUS
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Black locust (Robinia pseudoacada L.) clonal seed orchards in Hungary
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作者 Károly Redei Zoltán Osváth-Bujtás Irina Veperdi 《Forestry Studies in China》 CAS 2006年第4期47-50,共4页
Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its impor- tance is increasing in many countries. The main aim of the discussed new selection programme i... Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its impor- tance is increasing in many countries. The main aim of the discussed new selection programme is to identify black locust clones with good performance and good form for setting up clonal seed orchards. As a result of selection programme 16 new black locust clones have been improved. In spring 2002 a black locust seed orchard was established with the newly selected clones. About 40% of the plants can be considered to belong to the height growth rate class 1 and 2. Hungary was the first country where micropropagated black locust planting material was used for seed orchard establishment. 展开更多
关键词 black locust MICROPROPAGATION clonal seed orchards
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基于CiteSpace的林木种子园研究进展
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作者 张胜利 代金玲 卢秀琴 《安徽农学通报》 2025年第1期73-80,共8页
为了解林木种子园的研究现状和研究热点,检索中国知网(CNKI)数据库1962-2023年林木种子园的相关研究文献,应用文献计量学方法,从年度发文量、载文期刊、基金项目、作者、研究机构、关键词、突现词及被引文献频次等方面进行定量分析。结... 为了解林木种子园的研究现状和研究热点,检索中国知网(CNKI)数据库1962-2023年林木种子园的相关研究文献,应用文献计量学方法,从年度发文量、载文期刊、基金项目、作者、研究机构、关键词、突现词及被引文献频次等方面进行定量分析。结果显示,林木种子园的关注度持续升高,年度发文量呈逐年上升趋势,但近年论文创新和深度有待加强;核心库载文量较高的期刊包括《南京林业大学学报》(自然科学版)、《林业科学研究》和《东北林业大学学报》等;基金项目主要来源于国家级科研基金和省级项目;张含国、张振和金国庆等作者在林木种子园研究领域发文量较多;机构包括中国林业科学院亚热带林业研究所、东北林业大学和华中农业大学等;结合关键词和突现词可知,林木种子园以杉木、马尾松和油松等材用树种居多,研究围绕家系、无性系的生长性状遗传变异和子代测定等内容展开;被引频次较高的文献主要研究林木种子园遗传育种及变异等方面。未来将进一步聚焦基因遗传、基因工程等领域进行多样化育种研究,为林木种子园建设提供参考。 展开更多
关键词 种子园 中国知网数据库 定量分析 文献计量学
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马尾松实生种子园子代测定和早期选择
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作者 严艳兵 《防护林科技》 2025年第1期57-62,83,共7页
为给马尾松(Pinus massoniana)高世代种子园遗传改良提供育种材料,以福建省上杭白砂国有林场马尾松实生种子园第2批自由授粉子代测定林为试验材料,调查其4年生和5年生家系树高、胸径和冠幅,通过方差分析估算各性状遗传参数,在此基础上利... 为给马尾松(Pinus massoniana)高世代种子园遗传改良提供育种材料,以福建省上杭白砂国有林场马尾松实生种子园第2批自由授粉子代测定林为试验材料,调查其4年生和5年生家系树高、胸径和冠幅,通过方差分析估算各性状遗传参数,在此基础上利用BLUP育种值初步选育优良家系,并对经过第2次疏伐的马尾松实生种子园5年生子代林进行测定。结果表明:单株材积超过未改良普通林分种子育苗造林林分的93%。树高、胸径、材积和冠幅在家系间遗传差异极显著(P<0.01);4个生长性状的家系遗传力分别为34.14%、34.67%、35.08%和45.81%,受中度遗传控制,在家系水平上具有较大的改良潜力;通过一般配合力分析,选出6个配合力较高的优良亲本;采用BLUP育种值估算法初步选出速生优良家系10个,入选率为15%,家系材积的现实增益可达18.07%;以大于优良家系材积群体均值为标准,选出48个优株,单株入选率约3.8%,入选的单株树高、胸径和单株材积现实增益分别为17.94%、31.57%和60.41%。马尾松实生种子园第2批自由授粉子代具有较高的材积增益,初步选出的优株可为下一轮改良补充亲本资源。 展开更多
关键词 马尾松 母本评价 生长性状 遗传测定 实生种子园 遗传增益
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Genetic Diversity and Mating System Analysis of Pinus massoniana in Second-Generation Clonal Seed Orchard
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作者 Tan Xiaomei Zhou Zhichun +1 位作者 Jin Guoqing Zhang Yi 《Chinese Forestry Science and Technology》 2012年第3期52-53,共2页
A total 61 clone parents and 320 open-pollination progenies from 8 clone individuals were identified by 12 polymorphic SSR loci.The result showed that the level of genetic diversity in progeny population was the same ... A total 61 clone parents and 320 open-pollination progenies from 8 clone individuals were identified by 12 polymorphic SSR loci.The result showed that the level of genetic diversity in progeny population was the same as in maternal population, progeny population had all alleles detected in maternal population,and there were not obvious surplus phenomenon of homozygotes in progeny population (F= 0.046).The south crown had more strobili than the north crown,however the genetic diversity of the north crown strobili was not substantially reduced.The progeny of neutral trees had the same level of genetic diversity as in the progeny of partial female trees,with the fixation index tended to be zero,which was accorded with Hardy-Weinberg equilibrium.The multilocus outcrossing rate in the seed orchard was 1.097,and there was no significant inbreeding between parents(t_m-t_s= - 0.031).The multilocus outcrossing rate and singlelocus outcrossing rate in the south crown were higher than that in the north crown.The multilocus outcrossing rate of partial female trees was the same as in neutral trees,and the neutral tree had no obvious declining outcrossing rate with reduced proportion of female and male strobilus compared with partial female tree, with the fixation index tended to be zero.The progeny of the second generation clonal seed orchard still had rich genetic diversity,gene exchange among clones was relatively sufficient,and biparental inbreeding was not significant. 展开更多
关键词 PINUS massoniana second generation seed orchard SSR genetic diversity OUTCROSSING rate INBREEDING index
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施肥对日本落叶松母树种子球果和形质特征的影响
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作者 王骞春 战金伟 +4 位作者 杨圆圆 李光 卜鹏图 陈若楠 冯健 《西南林业大学学报(自然科学)》 CAS 北大核心 2024年第3期10-17,共8页
以日本落叶松1.5代和2代种子园为研究对象,施用N肥、P肥、K肥,采用正交试验设计,调查并分析了施肥处理对日本落叶松种子园母树球果和种子特性的影响,有效提高落叶松种子园种子产量,满足生产上对落叶松良种的需求。结果表明:从球果形质... 以日本落叶松1.5代和2代种子园为研究对象,施用N肥、P肥、K肥,采用正交试验设计,调查并分析了施肥处理对日本落叶松种子园母树球果和种子特性的影响,有效提高落叶松种子园种子产量,满足生产上对落叶松良种的需求。结果表明:从球果形质特征上看,施肥可显著促进日本落叶松1.5代和2代种子园单果质量、球果纵径和横径的增加,其中,1.5代种子园中,“N(0.4 kg/株)+P(0.6 kg/株)+K(0.6 kg/株)”处理的单果质量和球果纵径最大,分别达到4.01g和3.14cm,分别为CK的1.79倍和1.37倍,“N(0.2kg/株)+P(0.6kg/株)+K(0.4kg/株)”处理的球果横径最大,达到2.26cm,为CK的1.1倍;2代种子园中,“N(0.2 kg/株)+P(0.8 kg/株)+K(0.6 kg/株)”处理的单果质量、球果纵径和横径等指标均较高,分别达到8.23g、3.74 cm和2.58 cm。从种子形质特征上看,施肥对日本落叶松1.5代、2代种子园种子形质指标的影响有限,施肥仅对1.5代种子园种子纵径、横径和侧径及2代种子园千粒质量有显著影响。较高的K肥和适量的N肥施用量有利于球果和种子的增大,日本落叶松种子园施肥较优方案为N肥0.4 kg/株、P肥0.6 kg/株、K肥0.6 kg/株。 展开更多
关键词 日本落叶松 球果 种子形质 种子园
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日本落叶松种子园和人工林优树自由授粉家系苗期选择
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作者 胡勐鸿 李万峰 +3 位作者 吕寻 戴小芬 李宗德 周卓玲 《森林工程》 北大核心 2024年第6期64-78,共15页
探究日本落叶松种子园和人工林优树自由授粉家系苗期生长性状遗传变异规律,为优良家系造林和后期遗传改良提供参考。以沙坝国家落叶松/云杉良种基地日本落叶松初级无性系种子园25个自由授粉家系和30个人工林优树自由授粉家系为试验材料... 探究日本落叶松种子园和人工林优树自由授粉家系苗期生长性状遗传变异规律,为优良家系造林和后期遗传改良提供参考。以沙坝国家落叶松/云杉良种基地日本落叶松初级无性系种子园25个自由授粉家系和30个人工林优树自由授粉家系为试验材料,种子园混合种子苗作为对照,田间试验采用随机完全区组试验设计,在沙坝国家落叶松云杉良种基地苗木培育圃进行苗期生长试验。数据采用SPSS 16.0进行方差分析、多重比较,并估算遗传力和遗传增益,以2年生苗地径、苗高、新梢生长量为指标,采用Brykin多性状综合评定法进行家系评价和选择。种子园、人工林优树自由授粉家系1年生苗和2年生苗生长性状(地径、苗高、2年生苗新梢生长量)具有丰富的遗传变异,生长性状间具有显著(P<0.05)或极显著(P<0.01)正相关关系,家系选择潜力大。种子园自由授粉家系2年生苗地径、苗高、新梢生长量的表型变异系数分别是27.61%、36.82%、44.28%,家系遗传力依次为0.76、0.79、0.79,人工林优树自由授粉家系地径、苗高、新梢生长量的表型变异系数分别是25.57%、32.94%、38.83%,家系遗传力依次为0.90、0.92、0.92,属较高遗传力。家系生长性状受强的遗传控制,具有一定的稳定遗传能力,家系选择的遗传增益显著。按照50%的入选率初步选择苗期表现好的优良家系,种子园有种1、种25、种8、种34、种26、种12、种16、种20、种2、种100、种7、种21、种101共13个家系入选,入选家系地径、苗高、新梢生长量的遗传增益达到1.89%、6.18%、7.65%;人工林优树自由授粉家系有优19、优8、优2、优28、优30、优3、优7、优1、优18、优6、优15、优26、优25、优24、优13共15个家系入选,入选家系的地径、苗高、新梢生长量的遗传增益分别是3.73%、9.31%、11.19%。不论种子园还是人工林优树自由授粉家系,采用Brykin综合评价法选择的优良家系恰好是将苗高最大的家系入选。因此,建议日本落叶松自由授粉家系苗期采用单一性状——苗高作为评价和选择指标,会更加直观、经济、有效。在50%的入选率下,种子园、人工林优树自由授粉家系苗期分别有13个和15个家系入选,苗期初选家系为造林试验和后期遗传改良提供参考,有效减小田间试验规模,提高选择效率。苗高作为评价和选择指标,在生产中更加直观、方便、实用。 展开更多
关键词 日本落叶松 种子园自由授粉家系 优树自由授粉家系 苗期选择
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日本落叶松种子园和优树自由授粉家系选择与利用研究 被引量:2
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作者 吕寻 李万峰 +3 位作者 胡勐鸿 戴小芬 成红梅 委霞 《西南林业大学学报(自然科学)》 CAS 北大核心 2024年第3期1-9,共9页
在甘肃小陇山沙坝落叶松/云杉良种基地,采用完全随机区组试验设计,对25个种子园的自由授粉家系和30个优树自由授粉家系在日本落叶松采伐迹地进行了更新造林试验,应用SPSS16.0和ASReml v3.0对造林9年生数据进行了统计分析。结果表明:不... 在甘肃小陇山沙坝落叶松/云杉良种基地,采用完全随机区组试验设计,对25个种子园的自由授粉家系和30个优树自由授粉家系在日本落叶松采伐迹地进行了更新造林试验,应用SPSS16.0和ASReml v3.0对造林9年生数据进行了统计分析。结果表明:不论是种子园自由授粉家系还是优树自由授粉家系试验林,树高、胸径、材积等性状家系间具有丰富的遗传变异,家系不同性状间具有显著的相关性,表型变异系数和遗传变异系数最大的是材积。种子园自由授粉家系试验林树高、胸径、材积的家系遗传力和单株遗传力为0.492、0.680、0.694和0.093、0.162、0.171,优树自由授粉家系试验林树高、胸径、材积的家系遗传力和单株遗传力为0.560、0.544、0.496和0.128、0.109、0.096,家系性状遗传力大于单株遗传力,家系性状受到中等以上的遗传控制,家系选择潜力大。根据种子园自由授粉家系亲本育种值指导初级无性系种子园去劣疏伐、改建1.5代种子园。以材积为主要选择指标,兼顾胸径、树高,在家系择基础上配合单株选择,从种子园自由授粉家系和优树自由授粉家系各选择20个优良单株构建高阶生产群体,高阶生产群体的预期遗传增益和现实增益分别达到25.74%、22.85%和122.59%、154.22%。从种子园自由授粉家系选择的50个单株作为构建遗传改良群体的应用材料,将优树自由授粉家系选择的45个单株作为育种群体的补充材料,构建遗传品质不断提高和遗传基础不断拓展的改良群体应用材料。 展开更多
关键词 日本落叶松 种子园 自由授粉 家系 优树 育种 遗传变异
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基于种实表型性状和SSR标记的杉木2代种子园遗传多样性分析
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作者 冷春晖 娄永峰 +3 位作者 谢三秀 朱柯帆 宋晓琛 肖复明 《中南林业科技大学学报》 CAS CSCD 北大核心 2024年第4期159-168,共10页
【目的】从表型和分子2个角度评估江西信丰县国家杉木良种基地杉木2代种子园的遗传多样性,明确种子园的遗传背景,为后期种子园的改良(去劣疏伐)、重建(亲本选择与配置)等提供依据,同时为开展杂交育种提供遗传信息。【方法】以江西信丰杉... 【目的】从表型和分子2个角度评估江西信丰县国家杉木良种基地杉木2代种子园的遗传多样性,明确种子园的遗传背景,为后期种子园的改良(去劣疏伐)、重建(亲本选择与配置)等提供依据,同时为开展杂交育种提供遗传信息。【方法】以江西信丰杉木2代种子园内51个建园无性系为研究对象,通过分析8个种实表型性状掌握种子园不同无性系间种实性状的差异,同时利用SSR标记从DNA水平进行遗传多样性评估。【结果】供试51个无性系在种实表型性状和DNA水平上均存在明显的变异和较丰富的遗传多样性。8个表型性状变异系数为10.53%~37.04%,其中球果和种子的平均变异系数分别为18.99%和14.01%,种子的变异系数较小,表明种子的稳定性要比球果的高;种实性状的Shannon-Weaver多样性信息指数平均为2.060,表明种实性状的多样性较丰富。在DNA水平,14个SSR标记在51个无性系中共扩增出68个等位位点,平均Shannon’s信息指数、平均Nei’s遗传多样性指数、平均观测杂合度和平均期望杂合度分别为0.925、0.506、0.486和0.511,表明信丰杉木2代种子园遗传多样性较丰富。基于种实表型性状和SSR标记均可将51个无性系分为两大类群,但两者的具体聚类结果存在较大差异,Mantel检验显示种实性状与SSR标记之间不存在相关性(r=0.064,P=0.870>0.05)。【结论】信丰杉木2代种子园遗传多样性较丰富,具有再选择空间和改良潜力。 展开更多
关键词 杉木 种子园 种实性状 SSR标记 遗传多样性
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基于种实性状的杉木第3代无性系种子园亲本评价
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作者 王欣 徐刚标 +3 位作者 黄帆 蒋宏春 张森强 张勰 《湖南林业科技》 2024年第1期10-17,共8页
林木种子园是重要的林木良种基地,其主要任务是生产遗传品质和播种品质优良的种子并保证稳产高产。本研究以湖南省会同县第3代杉木无性系种子园中的29个无性系为研究对象,调查分析无性系球果纵径、横径、体积、产量及种子千粒重、发芽... 林木种子园是重要的林木良种基地,其主要任务是生产遗传品质和播种品质优良的种子并保证稳产高产。本研究以湖南省会同县第3代杉木无性系种子园中的29个无性系为研究对象,调查分析无性系球果纵径、横径、体积、产量及种子千粒重、发芽率、涩粒率、霉变率、发芽起始时间、发芽持续时间等10个指标,采用方差分析和主成分分析方法综合评价无性系的结实能力。研究结果表明,10个性状指标在29个无性系之间的差异极显著(P<0.01)。其中,球果产量变异最大,变异系数为103.53%;种子霉变率(75.40%)次之;再次为种子发芽率(38.21%)。不同无性系间,球果的纵径、横径、体积之间呈极显著正相关;种子发芽率与种子涩粒率呈极显著负相关、与种子霉变率之间存在显著负相关;球果的产量与球果的纵径、横径、体积等性状相关性不显著;种子的发芽起始时间与发芽持续时间呈显著负相关。基于球果、种子性状主成分分析的结果,筛选出综合性状排名前9位的6、27、22、23、28、26、5、14、9号无性系,可为第3代杉木无性系种子园去劣留优提供依据。 展开更多
关键词 杉木 种子园 球果 种子 综合评价
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四川杉木2代种子园半同胞子代测定林生长性状的遗传变异分析 被引量:1
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作者 陈国全 朱元伟 +4 位作者 张小国 罗红 李俊 杨昌通 朱鹏 《四川林业科技》 2024年第1期66-74,共9页
种子园子代测定林是开展推进种子园向升级换代的必要途径,对杉木第2代无性系种子园半同胞子代测定林13年生生长性状进行了遗传变异分析,以为杉木3代种子园的建设提供基础数据,结果表明:子代测定林的胸径、树高和材积在家系间、区组间以... 种子园子代测定林是开展推进种子园向升级换代的必要途径,对杉木第2代无性系种子园半同胞子代测定林13年生生长性状进行了遗传变异分析,以为杉木3代种子园的建设提供基础数据,结果表明:子代测定林的胸径、树高和材积在家系间、区组间以及二者的交互效应均存在显著差异,胸径和材积的家系遗传力为0.279和0.250,具有开展选优的价值和潜力;且胸径变异系数显示无论是在个体间还是在家系间生长都还在持续分化的过程中。51个家系胸径、树高和材积总的平均值分别为17.18 cm、12.61 m和0.1592 m3,相比对照均值现实增益分别为3.56%、2.66%和8.00%,材积现实增益超过10%的家系数量达21个,其中材积最大的家系现实增益达到了31.43%,现实增益明显。依据材积和胸径变异系数进行的综合优良度分析显示,T71,T78,T122,T152,T106,T114和T88等24个家系综合表现优异,可作为第3代无性系种子园建园材料的主要候选。 展开更多
关键词 杉木 2代无性系种子园 子代测定 变异系数 现实增益
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果园生草对苹果叶片光合特性及果实品质的影响 被引量:1
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作者 马仲炼 马磊 +3 位作者 马永翠 田虹 黄先敏 邱彩玲 《西南农业学报》 CSCD 北大核心 2024年第7期1480-1487,共8页
[目的]探究4种生草模式对苹果叶片光合特性和果实品质形成的影响,并筛选出适宜昭通果园的生草种类。[方法]以红富士为研究对象,设置果园清耕对照,探讨野艾蒿(Artemisia lavandulaefolia DC)、星毛繁缕(Stellaria vestita Kurz.)、黑麦草... [目的]探究4种生草模式对苹果叶片光合特性和果实品质形成的影响,并筛选出适宜昭通果园的生草种类。[方法]以红富士为研究对象,设置果园清耕对照,探讨野艾蒿(Artemisia lavandulaefolia DC)、星毛繁缕(Stellaria vestita Kurz.)、黑麦草(Lolium perenne L.)以及白花车轴草(Trifolium repens Linn)种群对果树叶绿素含量、光合特性和果实品质的影响。[结果]白花车轴草处理可以提高叶片总叶绿素含量、净光合速率、胞间CO_(2)浓度、气孔导度和羧化效率,而水分利用率显著降低;野艾蒿处理能增大叶片气孔导度,一定程度上提高胞间CO_(2)浓度,总叶绿素含量和叶片羧化效率降低;星毛繁缕处理能提高胞间CO_(2)浓度,但总叶绿素含量和叶片蒸腾速率降低;黑麦草处理可以显著提高水分利用率和叶片羧化效率,显著降低果树叶片蒸腾速率。品质方面,与清耕相比,各生草处理可使苹果的固形物含量增加0.88%~13.87%,还原糖含量降低2.30%~15.71%,总抗氧化能力增加10.03%~40.92%,黑麦草处理可使果实过氧化氢含量降低41.80%,超氧阴离子清除能力提高12.92%。利用模糊隶属函数法分析,清耕、白花车轴草、野艾蒿、星毛繁缕、黑麦草各处理综合评价值分别为0.4858、0.6044、0.5548、0.4894、0.6059。[结论]不同的草种种群在一定程度上提高果树叶片光合能力,改善果实品质,对4种草种种群进行科学评价,有益于筛选出更为合理的果园生草类型。 展开更多
关键词 昭通苹果 果园生草 草种 光合特性 品质
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蒙古栎无性系种子园花量变异及空间分布
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作者 包颖 蔡艺伟 +1 位作者 陈建伟 程广有 《北华大学学报(自然科学版)》 CAS 2024年第1期23-28,共6页
以吉林森工临江林业有限公司金山林场蒙古栎无性系种子园植株为对象,调查分析蒙古栎无性系雌、雄花空间分布规律。结果表明:无性系间单株标准枝数和标准枝小枝数差异达显著水平,无性系间小枝雄花序数、小枝雌花数、雄花序总数、雌花总... 以吉林森工临江林业有限公司金山林场蒙古栎无性系种子园植株为对象,调查分析蒙古栎无性系雌、雄花空间分布规律。结果表明:无性系间单株标准枝数和标准枝小枝数差异达显著水平,无性系间小枝雄花序数、小枝雌花数、雄花序总数、雌花总数差异均达到极显著水平;依据雌、雄花数量将各无性系聚类成多花类群、中间类群和少花类群;蒙古栎种子园内,雌、雄花量上、下冠层间差异均达到显著水平,花量在树冠上、下层间的比为1∶0.27,说明雌、雄花绝大多数都分布在树冠上层;蒙古栎无性系雄花数量在树冠不同方向上(东、西、南、北)的比为1∶0.89∶0.92∶0.72,雌花量为1∶0.83∶0.87∶0.72。 展开更多
关键词 蒙古栎 种子园 花量 变异 空间分布
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不同代次杉木种子园子代中龄期生长测评
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作者 练伟春 吕宇宙 +3 位作者 杨伯群 刘伟新 郑会全 王润辉 《林业科技情报》 2024年第4期1-3,共3页
为测定评价杉木不同代次种子园子代生长情况,选择不同地区、不同代次杉木种子园子代材料营建生长测定林分,对林分中龄期的树高、胸径和单株材积进行统计分析,结果表明,不同代次子代间存在极显著的差异,达到统计学上的极显著水平,不同代... 为测定评价杉木不同代次种子园子代生长情况,选择不同地区、不同代次杉木种子园子代材料营建生长测定林分,对林分中龄期的树高、胸径和单株材积进行统计分析,结果表明,不同代次子代间存在极显著的差异,达到统计学上的极显著水平,不同代次子代生长差异真实存在;树高、胸径和单株材积不同代次子代生长由大到小的顺序一致,均为小坑2.5代>小坑1.5代>信丰3代>信丰2.5代>韶关普通种>信丰15代,小坑2.5代生长最优。本研究结果证明粤北杉木栽培区以本地高世代杉木种子园生产的子代更适合,生长表现更好。 展开更多
关键词 杉木 种子园 不同代次 生长测评
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