Bodily gestures,facial expressions,and intonations are argued to be notably important features of spoken languagewhich are opposed to written language.Bodily gestures with or without spoken words can influence the cla...Bodily gestures,facial expressions,and intonations are argued to be notably important features of spoken languagewhich are opposed to written language.Bodily gestures with or without spoken words can influence the clarity and density of expres-sion and involvement of listeners.Facial expressions whether or not correspond with exact thought could be"decoded"to influencethe extent of intelligibility of expression.Intonation can always reflect the mutual beliefs concerning the propositional content andstates of consciousness relating to the expression and interpretation.Therefore,these can considerably improve or abate the accura-cy of expression and interpretation of thought.展开更多
The ω-atracotoxin-Arlb toxin （ω-ACTX-Arl b） is one of the arthropod-selective peptide neurotoxins from the venom of Australian funnel-web spider Atrax robustus. The gene of Arlb was synthesized and cloned into pET...The ω-atracotoxin-Arlb toxin （ω-ACTX-Arl b） is one of the arthropod-selective peptide neurotoxins from the venom of Australian funnel-web spider Atrax robustus. The gene of Arlb was synthesized and cloned into pET-32a（＋） vector to allow expression of Arlb as a fusion protein with thioredoxin and the His-tag （rTrx-Arlb） in E. coli BL21 （DE3）. The optimal condition for inducing the expression ofrTrx-Arl b was 1.0 mmol L-1 IPTG for 6 h at 28℃. The fusion protein rTrx- Arlb was expressed in soluble form and was purified effectively by HisTrap HP affinity column and rpHLPC and a final yield of purified rTrx-Arlb was 95 mg from 1 000 mL E. coli culture. The LD50 values for Mythimna separate and Tenebrio molitor were 111.66 and 11.04 ug g-1 determined by injection of the purified rTrx-Arlb. The results indicated that the recombinant Arlb protein was successfully expressed in E. coli and it was high toxicity against tested insects.展开更多
[ Objective] The disulfide-rich conotoxin MrV1B was produced by simple and fast genetic engineering method, to find new efficient ways for the synthesis of natural active conotoxins. [Method] Primers of conotoxin gene...[ Objective] The disulfide-rich conotoxin MrV1B was produced by simple and fast genetic engineering method, to find new efficient ways for the synthesis of natural active conotoxins. [Method] Primers of conotoxin gene MrVIB were synthesized to construct expression vectors pET22b（ ＋ ）/His-Xa-MrVIB and pET32a/Trx-EK-MrV1B, which were transformed into BL21 （DE3）pLysS and expressed under induction by IPTG. Recombinant proteins were purified by affinity chromatography using Ni-NTA agarose column, and the expression of the recombinant proteins was analyzed by Tricine-SDS-PAGE electrophoresis. [ Result] The recombinant conotoxins His-Xa-MrVIB and Trx-EK-MrVIB were effectively expressed in E. coli, and purified by one-step affinity chromatography, and the purity of the recombinant conotoxins was greater than 90%. [ Conclusion] The conotoxin MrVIB was effectively secreted and expressed by genetic engineering method, which could solve the problems in chemical synthesis of conotoxins including low yield, high cost and difficult purification.展开更多
Successful English speeches include thoughts about the topic,the speech daft,and speech expression skill.From the data of Chinese college students participating in the"CCTV CUP"English Speech Contest and oth...Successful English speeches include thoughts about the topic,the speech daft,and speech expression skill.From the data of Chinese college students participating in the"CCTV CUP"English Speech Contest and other English speech contests,it can be seen that the problems in Chinese college students'English speech are mainly lack of original and unique thoughts,draft writing skills and speech expression skills.Training can be done from improving the 3 aspects.展开更多
文摘Bodily gestures,facial expressions,and intonations are argued to be notably important features of spoken languagewhich are opposed to written language.Bodily gestures with or without spoken words can influence the clarity and density of expres-sion and involvement of listeners.Facial expressions whether or not correspond with exact thought could be"decoded"to influencethe extent of intelligibility of expression.Intonation can always reflect the mutual beliefs concerning the propositional content andstates of consciousness relating to the expression and interpretation.Therefore,these can considerably improve or abate the accura-cy of expression and interpretation of thought.
基金supported by the National Natural Science Foundation of China (NSFC-30872033)the Program for New Century Excellent Talents in University from Ministry of Education, China (NCET-06-0867)
文摘The ω-atracotoxin-Arlb toxin （ω-ACTX-Arl b） is one of the arthropod-selective peptide neurotoxins from the venom of Australian funnel-web spider Atrax robustus. The gene of Arlb was synthesized and cloned into pET-32a（＋） vector to allow expression of Arlb as a fusion protein with thioredoxin and the His-tag （rTrx-Arlb） in E. coli BL21 （DE3）. The optimal condition for inducing the expression ofrTrx-Arl b was 1.0 mmol L-1 IPTG for 6 h at 28℃. The fusion protein rTrx- Arlb was expressed in soluble form and was purified effectively by HisTrap HP affinity column and rpHLPC and a final yield of purified rTrx-Arlb was 95 mg from 1 000 mL E. coli culture. The LD50 values for Mythimna separate and Tenebrio molitor were 111.66 and 11.04 ug g-1 determined by injection of the purified rTrx-Arlb. The results indicated that the recombinant Arlb protein was successfully expressed in E. coli and it was high toxicity against tested insects.
基金Supported by Natural Science Foundation of China(81560611)Natural Science Foundation of Hainan Province(No.317170)
文摘[ Objective] The disulfide-rich conotoxin MrV1B was produced by simple and fast genetic engineering method, to find new efficient ways for the synthesis of natural active conotoxins. [Method] Primers of conotoxin gene MrVIB were synthesized to construct expression vectors pET22b（ ＋ ）/His-Xa-MrVIB and pET32a/Trx-EK-MrV1B, which were transformed into BL21 （DE3）pLysS and expressed under induction by IPTG. Recombinant proteins were purified by affinity chromatography using Ni-NTA agarose column, and the expression of the recombinant proteins was analyzed by Tricine-SDS-PAGE electrophoresis. [ Result] The recombinant conotoxins His-Xa-MrVIB and Trx-EK-MrVIB were effectively expressed in E. coli, and purified by one-step affinity chromatography, and the purity of the recombinant conotoxins was greater than 90%. [ Conclusion] The conotoxin MrVIB was effectively secreted and expressed by genetic engineering method, which could solve the problems in chemical synthesis of conotoxins including low yield, high cost and difficult purification.
文摘Successful English speeches include thoughts about the topic,the speech daft,and speech expression skill.From the data of Chinese college students participating in the"CCTV CUP"English Speech Contest and other English speech contests,it can be seen that the problems in Chinese college students'English speech are mainly lack of original and unique thoughts,draft writing skills and speech expression skills.Training can be done from improving the 3 aspects.
