Traditional Chinese preserved egg products have exhibited some anti-inflammatory effects,but their mechanisms of action remain unknown.This study aimed to investigate the anti-inflammatory effects of preserved egg whi...Traditional Chinese preserved egg products have exhibited some anti-inflammatory effects,but their mechanisms of action remain unknown.This study aimed to investigate the anti-inflammatory effects of preserved egg white(PEW)treatment on dextran sulfate sodium(DSS)-induced colitis in mice and the underlying mechanisms.The results showed that treatment with PEW in mice with DSS-induced colitis for 14 days effectively improved the clinical signs,inhibited the secretion and gene expression of pro-inflammatory cytokines,and reduced myeloperoxidase(MPO)activity and oxidative stress levels.In addition,western blotting results showed that PEW significantly suppressed DSS-induced phosphorylation levels of nuclear factor-kappa B(NF-κB)p65 and p38 mitogen-activated protein kinase(MAPK)in colon tissues of mice with colitis.PEW also enhanced the production of short-chain fatty acids(SCFAs)and modulated gut microbiota composition in mice with DSS-induced colitis,including increasing the relative abundance of beneficial bacteria Lachnospiraceae,Ruminococcaceae and Muribaculaceae,and reducing the relative abundance of harmful bacteria Proteobacteria.Taken together,our study demonstrated that preserved egg white could alleviate DSS-induced colitis in mice through the reduction of oxidative stress,modulation of inflammatory cytokines,NF-κB,MAPK and gut microbiota composition.展开更多
The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985-1998. Several clone...The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985-1998. Several clones derived from each batch of resting eggs were cultured under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed.展开更多
通过对平湖糟蛋样品前处理方式以及乙醇测定反应体系的优化,建立了重铬酸钾分光光度法测定平湖糟蛋中乙醇含量的方法。平湖糟蛋样品中的乙醇经蒸馏法充分提取,提取液在5.0mL重铬酸钾溶液(50g/L)和2.5mL硫酸溶液(50∶50,v/v)介质中于100...通过对平湖糟蛋样品前处理方式以及乙醇测定反应体系的优化,建立了重铬酸钾分光光度法测定平湖糟蛋中乙醇含量的方法。平湖糟蛋样品中的乙醇经蒸馏法充分提取,提取液在5.0mL重铬酸钾溶液(50g/L)和2.5mL硫酸溶液(50∶50,v/v)介质中于100℃水浴反应15min,恢复室温后采用分光光度法在585nm处测定吸光度值。该方法能较好排除基质效应影响,测定结果准确度高,乙醇含量在0.00~250.00g/kg范围内线性关系良好,相关系数大于0.999,加标回收率为96.2%~99.5%,精密度(relative standard deviation,RSD)为0.69%~2.28%。该方法操作简单、准确度高、回收率好,能准确地对糟蛋样品中乙醇进行定量检测。展开更多
Pidan or century egg, also known as preserved egg, is one of the most traditional and popular egg products in China. The crack detection of preserved eggshell is very important to guarantee its quality. In this study,...Pidan or century egg, also known as preserved egg, is one of the most traditional and popular egg products in China. The crack detection of preserved eggshell is very important to guarantee its quality. In this study, we develop an image algorithm for preserved eggshell＇s crack detection by using natural light and polarized image. Four features including crack length, crack state coefficient, maximum projection and angular point are extracted from the natural light image by morphology calculus algorithms. The support vector machines（SVM） model with radial basis kernel function is established using the four features with an accuracy of about 92%. The detection accuracy is improved to 94% by using a new characteristic parameter of crack length on polarization image. The Multi-information fusion analysis indicates the potential for cracks detection by a real-time synthesis imaging system.展开更多
基金financially supported by the Chinese National Natural Science Funds (31772043)the Fundamental Research Funds for the Central Universities (2662018JC021)
文摘Traditional Chinese preserved egg products have exhibited some anti-inflammatory effects,but their mechanisms of action remain unknown.This study aimed to investigate the anti-inflammatory effects of preserved egg white(PEW)treatment on dextran sulfate sodium(DSS)-induced colitis in mice and the underlying mechanisms.The results showed that treatment with PEW in mice with DSS-induced colitis for 14 days effectively improved the clinical signs,inhibited the secretion and gene expression of pro-inflammatory cytokines,and reduced myeloperoxidase(MPO)activity and oxidative stress levels.In addition,western blotting results showed that PEW significantly suppressed DSS-induced phosphorylation levels of nuclear factor-kappa B(NF-κB)p65 and p38 mitogen-activated protein kinase(MAPK)in colon tissues of mice with colitis.PEW also enhanced the production of short-chain fatty acids(SCFAs)and modulated gut microbiota composition in mice with DSS-induced colitis,including increasing the relative abundance of beneficial bacteria Lachnospiraceae,Ruminococcaceae and Muribaculaceae,and reducing the relative abundance of harmful bacteria Proteobacteria.Taken together,our study demonstrated that preserved egg white could alleviate DSS-induced colitis in mice through the reduction of oxidative stress,modulation of inflammatory cytokines,NF-κB,MAPK and gut microbiota composition.
文摘The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985-1998. Several clones derived from each batch of resting eggs were cultured under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed.
文摘通过对平湖糟蛋样品前处理方式以及乙醇测定反应体系的优化,建立了重铬酸钾分光光度法测定平湖糟蛋中乙醇含量的方法。平湖糟蛋样品中的乙醇经蒸馏法充分提取,提取液在5.0mL重铬酸钾溶液(50g/L)和2.5mL硫酸溶液(50∶50,v/v)介质中于100℃水浴反应15min,恢复室温后采用分光光度法在585nm处测定吸光度值。该方法能较好排除基质效应影响,测定结果准确度高,乙醇含量在0.00~250.00g/kg范围内线性关系良好,相关系数大于0.999,加标回收率为96.2%~99.5%,精密度(relative standard deviation,RSD)为0.69%~2.28%。该方法操作简单、准确度高、回收率好,能准确地对糟蛋样品中乙醇进行定量检测。
基金Supported by the Fundamental Funds for Central University(2662014BQ062)
文摘Pidan or century egg, also known as preserved egg, is one of the most traditional and popular egg products in China. The crack detection of preserved eggshell is very important to guarantee its quality. In this study, we develop an image algorithm for preserved eggshell＇s crack detection by using natural light and polarized image. Four features including crack length, crack state coefficient, maximum projection and angular point are extracted from the natural light image by morphology calculus algorithms. The support vector machines（SVM） model with radial basis kernel function is established using the four features with an accuracy of about 92%. The detection accuracy is improved to 94% by using a new characteristic parameter of crack length on polarization image. The Multi-information fusion analysis indicates the potential for cracks detection by a real-time synthesis imaging system.
