Objective To explore the immunological characteristics of natural resis tance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area Methods Passive transfer of sera from uninfe...Objective To explore the immunological characteristics of natural resis tance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area Methods Passive transfer of sera from uninfected laboratory bred MF (BM F) to albinao mice (AM) was performed to observe the acquired protection Sodiu m dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) and enzymelin ked immunoblotting (ELIB) methods were used to recognize 4 different lifecycle stage antigens of S japonicum by sera from wild MF (WMF), BMF, BMFi3 and BMFi9 Tests were also performed on in vitro killing effect of sera and/or lymphocytes from BMF an d WMF to schistosomulae; quantitative determination of C3 and C4 by immuno turb idometry, and interleukin4 (IL4) and antibodies to the 4 stage antigens in s era from WMF, BMF and infected BMF by ELISA Results Compared with the control group, stool eggs per gram (EPG) of A M in the test group was significantly reduced by 8154%, miracidium hatching rate, by 5 0 67%, liver egg counts, by 7207%, the diameter of hepatic egg granuloma, by 7 0 39?m Western blotting probed with the 4 MF sera all revealed 7 specific ban d s for SSA, 3 for AWA and SEA, but none for CA antigens The sera and/or lymphoc yte s from WMF and BMF gave obvious killing effects on schistosomulae with an adjust ed death rate of 6412%-7883% The levels ofnatural antibodiesproduced b y MF to S japonicum were in the following order: antiSSA>antiAWA>antiS EA>an tiCA, all of which increased significantly after the infection Serum levels o f C3, C4 and IL4 in uninfected BMF were significantly higher than those in AM After infection, levels of C3 and C4 were further increased respective ly by 7283% and 29549% in the 4th week and IL4 by 30383% in the 9th day Conclusions Immunological characteristics of innate resistance in M f o rtis to S japonicum infection were existed with no significant difference betw een WMF and BMF展开更多
为了准确评估特高压交流(UHV AC)输电线路的无线电干扰(RI)水平,利用模态分析法对多导体试验导线进行理论分析。推导得出试验导线终端接3种典型的阻抗网络时,导线正下方的无线电干扰电场强度分布特性,并对两端开路情况下的驻波进行了实...为了准确评估特高压交流(UHV AC)输电线路的无线电干扰(RI)水平,利用模态分析法对多导体试验导线进行理论分析。推导得出试验导线终端接3种典型的阻抗网络时,导线正下方的无线电干扰电场强度分布特性,并对两端开路情况下的驻波进行了实际测量。再根据武汉特高压交流试验基地中线路的布置情况,结合短线路的无线电干扰特性,对试验导线所测得的大雨条件下0.5 MHz的无线电干扰值进行数学反推,得到其激发函数值。利用该激发函数值对实际已运行的晋东南—南阳—荆门特高压输电线路的无线电干扰特性进行预测分析和实测对比。实验结果表明:当施加1 050 k V电压时,8×LGJ–500/35导线的激发函数值为39.3 d B;当测试点与中相导线的距离<60 m时,无线电干扰电场强度实测值与激发函数预测值之间的误差基本<2 d B。因此,可以利用数学反推来计算试验线段的激发函数值。展开更多
文摘Objective To explore the immunological characteristics of natural resis tance to Schistosoma japonicum infection in Microtus fortis (MF) living in the Dongting Lake area Methods Passive transfer of sera from uninfected laboratory bred MF (BM F) to albinao mice (AM) was performed to observe the acquired protection Sodiu m dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) and enzymelin ked immunoblotting (ELIB) methods were used to recognize 4 different lifecycle stage antigens of S japonicum by sera from wild MF (WMF), BMF, BMFi3 and BMFi9 Tests were also performed on in vitro killing effect of sera and/or lymphocytes from BMF an d WMF to schistosomulae; quantitative determination of C3 and C4 by immuno turb idometry, and interleukin4 (IL4) and antibodies to the 4 stage antigens in s era from WMF, BMF and infected BMF by ELISA Results Compared with the control group, stool eggs per gram (EPG) of A M in the test group was significantly reduced by 8154%, miracidium hatching rate, by 5 0 67%, liver egg counts, by 7207%, the diameter of hepatic egg granuloma, by 7 0 39?m Western blotting probed with the 4 MF sera all revealed 7 specific ban d s for SSA, 3 for AWA and SEA, but none for CA antigens The sera and/or lymphoc yte s from WMF and BMF gave obvious killing effects on schistosomulae with an adjust ed death rate of 6412%-7883% The levels ofnatural antibodiesproduced b y MF to S japonicum were in the following order: antiSSA>antiAWA>antiS EA>an tiCA, all of which increased significantly after the infection Serum levels o f C3, C4 and IL4 in uninfected BMF were significantly higher than those in AM After infection, levels of C3 and C4 were further increased respective ly by 7283% and 29549% in the 4th week and IL4 by 30383% in the 9th day Conclusions Immunological characteristics of innate resistance in M f o rtis to S japonicum infection were existed with no significant difference betw een WMF and BMF
文摘为了准确评估特高压交流(UHV AC)输电线路的无线电干扰(RI)水平,利用模态分析法对多导体试验导线进行理论分析。推导得出试验导线终端接3种典型的阻抗网络时,导线正下方的无线电干扰电场强度分布特性,并对两端开路情况下的驻波进行了实际测量。再根据武汉特高压交流试验基地中线路的布置情况,结合短线路的无线电干扰特性,对试验导线所测得的大雨条件下0.5 MHz的无线电干扰值进行数学反推,得到其激发函数值。利用该激发函数值对实际已运行的晋东南—南阳—荆门特高压输电线路的无线电干扰特性进行预测分析和实测对比。实验结果表明:当施加1 050 k V电压时,8×LGJ–500/35导线的激发函数值为39.3 d B;当测试点与中相导线的距离<60 m时,无线电干扰电场强度实测值与激发函数预测值之间的误差基本<2 d B。因此,可以利用数学反推来计算试验线段的激发函数值。
