Four new dammarane-type triterpenoid saponins,namely notoginsenosides SFt1-SFt4(1-4)were isolated from the steamed leaves of Panax notoginseng(Burk.)F.H.Chen(Araliaceae),together with 17 known saponins.Their structure...Four new dammarane-type triterpenoid saponins,namely notoginsenosides SFt1-SFt4(1-4)were isolated from the steamed leaves of Panax notoginseng(Burk.)F.H.Chen(Araliaceae),together with 17 known saponins.Their structures were established on the basis of detailed spectroscopic analyses and acidic hydrolysis.The known ginsenosides Rk2 and Rh3 were obtained from P.notoginseng for the first time.All of these new saponins showed no in vitro cytotoxicity against five human cancer cell lines(HL-60,SMMC-7712,A-549,MCF-7,and SW480).展开更多
Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Al...Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-glucanase.The amount and purity of C-K obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were optimized.Results β-Glucanase can be immobilized and reused with either of the entrapment.However,using Alg 1 resulted in higher enzyme activity than Alg 2.The optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4–6 h;and the optimal concentration of the crosslinking agent was 6%– 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-K from SLPN.展开更多
本研究从性状、pH、相对密度、装量差异、微生物限度、鉴别、含量测定等方面对三七茎叶皂苷口服液(Oral liquid of saponins of stems and leaves of Panax notoginseng,LSPN)进行质量控制研究。利用薄层色谱(thin layer chromatography...本研究从性状、pH、相对密度、装量差异、微生物限度、鉴别、含量测定等方面对三七茎叶皂苷口服液(Oral liquid of saponins of stems and leaves of Panax notoginseng,LSPN)进行质量控制研究。利用薄层色谱(thin layer chromatography,TLC)法,鉴定其主要成分;利用高效液相色谱-蒸发光散射(high-performance liquid chromatography with evaporative light scattering detector,HPLC-ELSD)法,建立LSPN中人参皂苷Rb1和Rb3含量的同步分析方法。HPLC-ELSD的色谱条件:色谱柱为GL,InertSustain C18柱(5μm,250×4.6 mm Column);流动相为乙腈(A)-水(B),采用梯度洗脱;进样量:10 L;人参皂苷Rb1和Rb3保留时间分别为13.264 min和15.415 min。ELSD运行参数:氮气流速为1.64 slpm,气化温度为48℃,蒸发温度为80℃。结果表明,LSPN为黄绿色的澄清液体,味苦,pH、相对密度和微生物限度均符合标准要求。人参皂苷Rb1和Rb3的检出限(limit of detection,LOD)均为10 g/mL,定量限(limit of quantitation,LOQ)均为20 g/mL,均在20~500 g/mL内线性关系良好;回归方程分别为Y=1.58X+1.72(r=0.999904)和Y=1.60X+1.64(r=0.999628)。人参皂苷Rb1和Rb3的平均加样回收率分别为101.25%和100.42%,RSD分别为2.71%和1.12%。该法准确,精密度、重现性好,可用于LSPN中人参皂苷Rb1和Rb3的含量测定。展开更多
基金This work was supported by the 973 Program of Ministry of Science and Technology of China(2011CB915503).
文摘Four new dammarane-type triterpenoid saponins,namely notoginsenosides SFt1-SFt4(1-4)were isolated from the steamed leaves of Panax notoginseng(Burk.)F.H.Chen(Araliaceae),together with 17 known saponins.Their structures were established on the basis of detailed spectroscopic analyses and acidic hydrolysis.The known ginsenosides Rk2 and Rh3 were obtained from P.notoginseng for the first time.All of these new saponins showed no in vitro cytotoxicity against five human cancer cell lines(HL-60,SMMC-7712,A-549,MCF-7,and SW480).
基金ZHAO Yu-qing was supportive with a Liaoning Modernization TCM grant (LN403004),China
文摘Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-glucanase.The amount and purity of C-K obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were optimized.Results β-Glucanase can be immobilized and reused with either of the entrapment.However,using Alg 1 resulted in higher enzyme activity than Alg 2.The optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4–6 h;and the optimal concentration of the crosslinking agent was 6%– 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-K from SLPN.
文摘本研究从性状、pH、相对密度、装量差异、微生物限度、鉴别、含量测定等方面对三七茎叶皂苷口服液(Oral liquid of saponins of stems and leaves of Panax notoginseng,LSPN)进行质量控制研究。利用薄层色谱(thin layer chromatography,TLC)法,鉴定其主要成分;利用高效液相色谱-蒸发光散射(high-performance liquid chromatography with evaporative light scattering detector,HPLC-ELSD)法,建立LSPN中人参皂苷Rb1和Rb3含量的同步分析方法。HPLC-ELSD的色谱条件:色谱柱为GL,InertSustain C18柱(5μm,250×4.6 mm Column);流动相为乙腈(A)-水(B),采用梯度洗脱;进样量:10 L;人参皂苷Rb1和Rb3保留时间分别为13.264 min和15.415 min。ELSD运行参数:氮气流速为1.64 slpm,气化温度为48℃,蒸发温度为80℃。结果表明,LSPN为黄绿色的澄清液体,味苦,pH、相对密度和微生物限度均符合标准要求。人参皂苷Rb1和Rb3的检出限(limit of detection,LOD)均为10 g/mL,定量限(limit of quantitation,LOQ)均为20 g/mL,均在20~500 g/mL内线性关系良好;回归方程分别为Y=1.58X+1.72(r=0.999904)和Y=1.60X+1.64(r=0.999628)。人参皂苷Rb1和Rb3的平均加样回收率分别为101.25%和100.42%,RSD分别为2.71%和1.12%。该法准确,精密度、重现性好,可用于LSPN中人参皂苷Rb1和Rb3的含量测定。
