Background: Due to their delicious taste, high nutritional content, and health benefits, fruit juices are well-known drinks in many countries and are now an essential component of the modern diet. Objective: Determini...Background: Due to their delicious taste, high nutritional content, and health benefits, fruit juices are well-known drinks in many countries and are now an essential component of the modern diet. Objective: Determining the microbiological quality of both packaged and freshly made fruit and milk juices. Method: The spread-plate approach was employed to isolate and count the bacteria. 90 ml of sterile peptone water were blended with 10 ml of well-mixed, packed, and freshly made fruit juices. The samples were sequentially diluted (101 - 105) in accordance with the Indian Manual of Food Microbiological Testing Methods. Results: From eight samples of imported packaged fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 1.39 × 102, and 2 × 102 CFU/ml, respectively. In contrast, from three samples of locally produced fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 5.83 × 102, and 2.73 × 103 CFU/ml, respectively. Four samples of handmade prepared fruit and milk juices had a mean of total coliform, staphylococci, and viable bacterial count of 1.441 × 104, 4.1 × 103, and 2.35 × 105 CFU/ml, respectively. Conclusion: 33.3% of the results from microbiological analysis of freshly made fruit and milk juices met the permissible range of the Revised Microbiological Standards for Fruit and Vegetables and Their Products, which were published in 2018 and as well as the Hong Kong Center for Food Safety, whereas 66.7% of the microbiological analyses of freshly prepared fruit and milk juices were above the permissible reference range of GSO standard 2000. 12.5% of the investigated imported and packed fruits and milk juices had one failed test (TSC), which was above the acceptable limit, 87.5% of the tested samples of fruit and milk juices fulfilled the necessary standards of TCC, TVBC, and TSC. 100% of the tested locally manufactured fruit and milk juices complied with TSC, TCC, and TVBC requirements. All investigations showed that freshly made fruit and milk juices were heavily contaminated (Total viable bacterial count, total coliform count, and total staphylococcus count). .展开更多
[Objectives]This study was conducted to select suitable mouse beddings from commercial corncob bedding(A-E)produced by five different manufacturers.[Methods]The physical properties and microbial indexes of the five ki...[Objectives]This study was conducted to select suitable mouse beddings from commercial corncob bedding(A-E)produced by five different manufacturers.[Methods]The physical properties and microbial indexes of the five kinds of corncob bedding were studied by means of visual inspection,dust content determination,moisture content determination,water absorption tests and microbial detection.[Results]In visual inspection,corncob bedding A was better than other four kinds.During dust content determination,corncob bedding D was higher than other 4 kinds(1.83%±0.2983%,P<0.05).In terms of moisture content,the moisture contents of the five kinds of corncob bedding were less than or equal to 10%.In the water absorption test,the water absorption capacities of the five kinds of bedding before autoclaving were all greater than 150%,and the water absorption of corncob bedding D after autoclaving was better than other four kinds(171.0±5.3,P<0.05).During microbial detection,the total number of colonies of corncob bedding B before autoclaving was higher than those of other four types(1.42×10^(5)±4.3×10^(4),P<0.05).The five kinds of commercial corncob bedding have different physical parameters and microbial conditions,and each has its own advantages and disadvantages.When purchasing and selecting corncob bedding,it is necessary to comprehensively consider the needs of daily feeding work,and choose bedding according to the effects on the health and welfare of experimental animals.[Conclusions]This study provides a reference for selecting suitable corncob bedding for mouse.展开更多
食源性致病菌一直是我国食品安全的危害之一,如何有效控制食品中食源性致病菌的繁殖一直是研究热点。该研究以等离子体活性乳酸溶液(Plasma-Activated Lactic Acid,PALA)体积分数、活化时间以及反应时间的单因素实验为基础,以大肠埃希...食源性致病菌一直是我国食品安全的危害之一,如何有效控制食品中食源性致病菌的繁殖一直是研究热点。该研究以等离子体活性乳酸溶液(Plasma-Activated Lactic Acid,PALA)体积分数、活化时间以及反应时间的单因素实验为基础,以大肠埃希氏菌、沙门氏菌、金黄色葡萄球菌及单核增生李斯特菌等菌种为模型,菌落总数为响应值,研究PALA对4种食源性致病菌的影响。研究结果表明:在一定范围内,4种食源性致病菌的杀灭率均随PALA体积分数的升高、活化时间以及反应时间的延长而增大;采用活化120 s、体积分数为0.2%的PALA与4种食源性致病菌反应10 min,约可杀灭纯培养大肠埃希氏菌7.71 lg CFU/mL、沙门氏菌4.52 lg CFU/mL、金黄色葡萄球菌4.03 lg CFU/mL、单核增生李斯特菌3.90 lg CFU/mL。对4种纯培养食源性致病菌的杀灭率达99.9%以上(P<0.05),可杀灭3.9个对数值以上菌落总数的致病菌。综上所述,PALA可快速、高效杀灭常见食源性致病菌。展开更多
文摘Background: Due to their delicious taste, high nutritional content, and health benefits, fruit juices are well-known drinks in many countries and are now an essential component of the modern diet. Objective: Determining the microbiological quality of both packaged and freshly made fruit and milk juices. Method: The spread-plate approach was employed to isolate and count the bacteria. 90 ml of sterile peptone water were blended with 10 ml of well-mixed, packed, and freshly made fruit juices. The samples were sequentially diluted (101 - 105) in accordance with the Indian Manual of Food Microbiological Testing Methods. Results: From eight samples of imported packaged fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 1.39 × 102, and 2 × 102 CFU/ml, respectively. In contrast, from three samples of locally produced fruit and milk juice, the average of total coliform, staphylococci, and viable bacterial counts were zero, 5.83 × 102, and 2.73 × 103 CFU/ml, respectively. Four samples of handmade prepared fruit and milk juices had a mean of total coliform, staphylococci, and viable bacterial count of 1.441 × 104, 4.1 × 103, and 2.35 × 105 CFU/ml, respectively. Conclusion: 33.3% of the results from microbiological analysis of freshly made fruit and milk juices met the permissible range of the Revised Microbiological Standards for Fruit and Vegetables and Their Products, which were published in 2018 and as well as the Hong Kong Center for Food Safety, whereas 66.7% of the microbiological analyses of freshly prepared fruit and milk juices were above the permissible reference range of GSO standard 2000. 12.5% of the investigated imported and packed fruits and milk juices had one failed test (TSC), which was above the acceptable limit, 87.5% of the tested samples of fruit and milk juices fulfilled the necessary standards of TCC, TVBC, and TSC. 100% of the tested locally manufactured fruit and milk juices complied with TSC, TCC, and TVBC requirements. All investigations showed that freshly made fruit and milk juices were heavily contaminated (Total viable bacterial count, total coliform count, and total staphylococcus count). .
文摘[Objectives]This study was conducted to select suitable mouse beddings from commercial corncob bedding(A-E)produced by five different manufacturers.[Methods]The physical properties and microbial indexes of the five kinds of corncob bedding were studied by means of visual inspection,dust content determination,moisture content determination,water absorption tests and microbial detection.[Results]In visual inspection,corncob bedding A was better than other four kinds.During dust content determination,corncob bedding D was higher than other 4 kinds(1.83%±0.2983%,P<0.05).In terms of moisture content,the moisture contents of the five kinds of corncob bedding were less than or equal to 10%.In the water absorption test,the water absorption capacities of the five kinds of bedding before autoclaving were all greater than 150%,and the water absorption of corncob bedding D after autoclaving was better than other four kinds(171.0±5.3,P<0.05).During microbial detection,the total number of colonies of corncob bedding B before autoclaving was higher than those of other four types(1.42×10^(5)±4.3×10^(4),P<0.05).The five kinds of commercial corncob bedding have different physical parameters and microbial conditions,and each has its own advantages and disadvantages.When purchasing and selecting corncob bedding,it is necessary to comprehensively consider the needs of daily feeding work,and choose bedding according to the effects on the health and welfare of experimental animals.[Conclusions]This study provides a reference for selecting suitable corncob bedding for mouse.
文摘食源性致病菌一直是我国食品安全的危害之一,如何有效控制食品中食源性致病菌的繁殖一直是研究热点。该研究以等离子体活性乳酸溶液(Plasma-Activated Lactic Acid,PALA)体积分数、活化时间以及反应时间的单因素实验为基础,以大肠埃希氏菌、沙门氏菌、金黄色葡萄球菌及单核增生李斯特菌等菌种为模型,菌落总数为响应值,研究PALA对4种食源性致病菌的影响。研究结果表明:在一定范围内,4种食源性致病菌的杀灭率均随PALA体积分数的升高、活化时间以及反应时间的延长而增大;采用活化120 s、体积分数为0.2%的PALA与4种食源性致病菌反应10 min,约可杀灭纯培养大肠埃希氏菌7.71 lg CFU/mL、沙门氏菌4.52 lg CFU/mL、金黄色葡萄球菌4.03 lg CFU/mL、单核增生李斯特菌3.90 lg CFU/mL。对4种纯培养食源性致病菌的杀灭率达99.9%以上(P<0.05),可杀灭3.9个对数值以上菌落总数的致病菌。综上所述,PALA可快速、高效杀灭常见食源性致病菌。
