In previous studies,researchers have used singing to treat respiratory function in patients with spinal cord injury.However,few studies have examined the way in which vocal training affects respiratory neural plastici...In previous studies,researchers have used singing to treat respiratory function in patients with spinal cord injury.However,few studies have examined the way in which vocal training affects respiratory neural plasticity in patients with spinal cord injury.Vocal respiratory training(VRT)is a type of vocal muscle-related treatment that is often a component of music therapy(MT)and focuses on strengthening respiratory muscles and improving lung function.In this randomized controlled study,we analyzed the therapeutic effects of VRT on respiratory dysfunction at 3 months after cervical spinal cord injury.Of an initial group of 37 patients,26 completed the music therapy intervention,which comprised five 30-minute sessions per week for 12 weeks.The intervention group(n=13)received VRT training delivered by professional certified music therapists.The control group(n=13)received respiratory physical therapy delivered by professional physical therapists.Compared with the control group,we observed a substantial increase in respiratory function in the intervention group after the 12-week intervention.Further,the nerve fiber bundles in the respiratory center in the medulla exhibited a trend towards increased diversification,with an increased number,path length,thickness,and density of nerve fiber bundles.These findings provide strong evidence for the effect of music therapeutic VRT on neural plasticity.This study was approved by the Ethics Committee of China Rehabilitation Research Center(approval No.2020-013-1)on April 1,2020,and was registered with the Chinese Clinical Trial Registry(registration No.Chi CTR2000037871)on September 2,2020.展开更多
The change of vocal flinction after vocal fold dehydration due to dryness was discussed along with the treatment eHect of different atomizing agents.Forty-eight staffs from The Central Hospital of Wuhan were recniited...The change of vocal flinction after vocal fold dehydration due to dryness was discussed along with the treatment eHect of different atomizing agents.Forty-eight staffs from The Central Hospital of Wuhan were recniited.All volunteers breathed dry air for vocal fold dehydration.After dry air inhalation,the subjects were randomly divided into four groups,with 12 cases each.Three groups were treatment groups,receiving 0.9%nonnal saline(IS),5%hypertonic saline(HS)and double-distilled water(SW)atomizing inhalation therapy,respectively,and the last group was the control group without treatment.Voice data were collected for all subjects before and immediately after dry air inhalation using the Multi-Dimensional Voice Program(MDVP)system.Atomizing inhalation therapy was given 10 min after dry air inhalation,and voice data were collected using MDVP system at the following time points after atomizing inhalation treatment:5 min,20 min,35 min,50 min,65 min,80 min,95 min,110 min.In the control group,voice data were collected at the same time points and compared with those of treatment groups.The vocal function parameters collected before and after dry air inhalation as well as after treatment were subjected to test using SPSS 16.0 software.In the four groups,jitter(fundamental frequency perturbation),shimmer(amplitude perturbation),and amplitude perturbation quotient(APQ)were significantly increased after dry air inhalation(P<0.05).In IS,HS and SW groups,after atomizing inhalation treatment,there was an obvious reduction in jitter,shimmer and APQ,showing significant differences as compared with those after dry air inhalation(P<0.05).Moreover,these parameters were significantly lower than those in the control group(P<0.05).The jitter,shimmer and APQ in the IS group were significantly lower than those in the HS and SW groups(P<0.05).We are led to a con elusion:Vocal fold dehydration induced by dryness can reduce the stability of voice;such decreased voice stability can be improved by atomizing inhalation therapy;without proper treatment,voice stability caused by vocal fold dehydration cannot heal spontaneously;of three atomizing agents namely,IS,HS and SW,IS had the best treatment effect for decreased voice stability caused by vocal fold dehydration.展开更多
Medialization thyroplasty or injection laryngoplasty for unilateral vocal fold paralysis cannot restore mobility of the vocal fold. Recent studies have shown that transplantation of mesenchymal stem cells is effective...Medialization thyroplasty or injection laryngoplasty for unilateral vocal fold paralysis cannot restore mobility of the vocal fold. Recent studies have shown that transplantation of mesenchymal stem cells is effective in the repair of nerve injuries. This study investigated wheth- er adipose-derived stem celt transplantation could repair recurrent laryngeal nerve injury. Rat models of recurrent laryngeal nerve injury were established by crushing with micro forceps. Adipose-derived mesenchymal stem cells （ADSCs; 8 ×105） or differentiated Schwann-like adipose-derived mesenchymal stem cells （dADSCs; 8×105） or extracellular matrix were injected at the site of injury. At 2, 4 and 6 weeks post-surgery, a higher density of myelinated nerve fiber, thicker myelin sheath, improved vocal fold movement, better recovery of nerve conduction capacity and reduced thyroarytenoid muscle atrophy were found in ADSCs and dADSCs groups compared with the extracellu- lar matrix group. The effects were more pronounced in the ADSCs group than in the dADSCs group. These experimental results indicated that ADSCs transplantation could be an early interventional strategy to promote regeneration after recurrent laryngeal nerve injury.展开更多
Background: Individuality in vocalizations may provide an effective tool for surveying populations of the Common Cuckoo(Cuculus canorus) but there remains few data on which technique to use to identify individuality. ...Background: Individuality in vocalizations may provide an effective tool for surveying populations of the Common Cuckoo(Cuculus canorus) but there remains few data on which technique to use to identify individuality. In this research, we compared the within-and between-individual variation in cuckoo calls using two different analytical methods, and discuss the feasibility of using call individuality to count male cuckoos within a population.Methods: We recorded vocalization from 13 males, and measured 15 spectro-temporal variables for each call. The majority of these call variables(n = 12) have greater variation between individuals than within individual. We first calculated the similarity(Pearson's R) for each paired calls in order to find a threshold that could distinguish calls emitted from the same or different males, and then counted the number of males based on this distinction. Second, we used the more widely accepted technique of discriminant function analysis(DFA) to identify individual male cuckoos, and compared the correct rate of classifying individuals between the two analytical methods.Results: Similarity of paired calls from the same male was significantly higher than from different males. Under a relatively broad threshold interval, we achieved a high(>90%) correct rate to distinguish calls and an accurate estimate of male numbers. Based on banded males(n = 3), we found the similarity of paired calls from different days was lower when compared with paired calls from the same day, but this change did not obscure individual identification, as similarity values of paired calls from different days were still larger than the threshold used to distinguish calls from the same or different males. DFA also yielded a high rate(91.9%) of correct classification of individuals.Conclusions: Our study suggests that identifying individual vocalizations can form the basis of an appropriate survey method for counting male cuckoos within a population, provided the performance of different analytical techniques are compared.展开更多
Ultrasonic communication in vertebrates is attracting increasing research interest.To determine if ultrasonic vocalization is common in birds,we recorded their vocalizations with ultrasound detectors in the Dongzhai N...Ultrasonic communication in vertebrates is attracting increasing research interest.To determine if ultrasonic vocalization is common in birds,we recorded their vocalizations with ultrasound detectors in the Dongzhai National Nature Reserve of Henan Province,China.We found varying degrees of high frequency components in the vocalizations of 14 species and in several of these species,the frequency of harmonics was up to the range of ultrasound.We suggest that more studies are required to determine whether the high frequency components in avian vocalizations have functions and what these functions are.In addition,the ability of birds to hear sounds in the high frequency range also requires re-examination.展开更多
41 cases still showing various symptoms and signs after having been operated on for intralaryngeal conditions were treated with Yiqi Kaiyin Tang (益气开音汤) the vital energy replenishing and voice restoring decoction...41 cases still showing various symptoms and signs after having been operated on for intralaryngeal conditions were treated with Yiqi Kaiyin Tang (益气开音汤) the vital energy replenishing and voice restoring decoction). The chief clinical manifestations were hoarseness of vioce, lack of strength for speech, feeling of dryness and pain in the throat with foreign body sensation or burning sensation, and aphonia. The treatment lasted 15-60 days, resulting in cure in 19 cases, improvement in 16 cases, and no response in 6 cases, making a total effectiveness rate of 85.4%.展开更多
基金supported by Scientific Research Project of Establishment of the Winter Olympics Sports Injury Rehabilitation Diagnosis and Treatment System and Green Channel Demonstration of China,No.2018YFF0301104(to JJL)China Rehabilitation Science Institute,No.2020cz-10(to WZW)Scientific Research Foundation of China Rehabilitation Research Center,No.2017zx-32(to XYZ)。
文摘In previous studies,researchers have used singing to treat respiratory function in patients with spinal cord injury.However,few studies have examined the way in which vocal training affects respiratory neural plasticity in patients with spinal cord injury.Vocal respiratory training(VRT)is a type of vocal muscle-related treatment that is often a component of music therapy(MT)and focuses on strengthening respiratory muscles and improving lung function.In this randomized controlled study,we analyzed the therapeutic effects of VRT on respiratory dysfunction at 3 months after cervical spinal cord injury.Of an initial group of 37 patients,26 completed the music therapy intervention,which comprised five 30-minute sessions per week for 12 weeks.The intervention group(n=13)received VRT training delivered by professional certified music therapists.The control group(n=13)received respiratory physical therapy delivered by professional physical therapists.Compared with the control group,we observed a substantial increase in respiratory function in the intervention group after the 12-week intervention.Further,the nerve fiber bundles in the respiratory center in the medulla exhibited a trend towards increased diversification,with an increased number,path length,thickness,and density of nerve fiber bundles.These findings provide strong evidence for the effect of music therapeutic VRT on neural plasticity.This study was approved by the Ethics Committee of China Rehabilitation Research Center(approval No.2020-013-1)on April 1,2020,and was registered with the Chinese Clinical Trial Registry(registration No.Chi CTR2000037871)on September 2,2020.
文摘The change of vocal flinction after vocal fold dehydration due to dryness was discussed along with the treatment eHect of different atomizing agents.Forty-eight staffs from The Central Hospital of Wuhan were recniited.All volunteers breathed dry air for vocal fold dehydration.After dry air inhalation,the subjects were randomly divided into four groups,with 12 cases each.Three groups were treatment groups,receiving 0.9%nonnal saline(IS),5%hypertonic saline(HS)and double-distilled water(SW)atomizing inhalation therapy,respectively,and the last group was the control group without treatment.Voice data were collected for all subjects before and immediately after dry air inhalation using the Multi-Dimensional Voice Program(MDVP)system.Atomizing inhalation therapy was given 10 min after dry air inhalation,and voice data were collected using MDVP system at the following time points after atomizing inhalation treatment:5 min,20 min,35 min,50 min,65 min,80 min,95 min,110 min.In the control group,voice data were collected at the same time points and compared with those of treatment groups.The vocal function parameters collected before and after dry air inhalation as well as after treatment were subjected to test using SPSS 16.0 software.In the four groups,jitter(fundamental frequency perturbation),shimmer(amplitude perturbation),and amplitude perturbation quotient(APQ)were significantly increased after dry air inhalation(P<0.05).In IS,HS and SW groups,after atomizing inhalation treatment,there was an obvious reduction in jitter,shimmer and APQ,showing significant differences as compared with those after dry air inhalation(P<0.05).Moreover,these parameters were significantly lower than those in the control group(P<0.05).The jitter,shimmer and APQ in the IS group were significantly lower than those in the HS and SW groups(P<0.05).We are led to a con elusion:Vocal fold dehydration induced by dryness can reduce the stability of voice;such decreased voice stability can be improved by atomizing inhalation therapy;without proper treatment,voice stability caused by vocal fold dehydration cannot heal spontaneously;of three atomizing agents namely,IS,HS and SW,IS had the best treatment effect for decreased voice stability caused by vocal fold dehydration.
基金supported by the National Natural Science Foundation of China,No.81470680,81170901the Natural Science Foundation of Beijing of China,No.7132053the Beijing Health Foundation of High-level Technical Personnel in China,No.2014-2-004
文摘Medialization thyroplasty or injection laryngoplasty for unilateral vocal fold paralysis cannot restore mobility of the vocal fold. Recent studies have shown that transplantation of mesenchymal stem cells is effective in the repair of nerve injuries. This study investigated wheth- er adipose-derived stem celt transplantation could repair recurrent laryngeal nerve injury. Rat models of recurrent laryngeal nerve injury were established by crushing with micro forceps. Adipose-derived mesenchymal stem cells （ADSCs; 8 ×105） or differentiated Schwann-like adipose-derived mesenchymal stem cells （dADSCs; 8×105） or extracellular matrix were injected at the site of injury. At 2, 4 and 6 weeks post-surgery, a higher density of myelinated nerve fiber, thicker myelin sheath, improved vocal fold movement, better recovery of nerve conduction capacity and reduced thyroarytenoid muscle atrophy were found in ADSCs and dADSCs groups compared with the extracellu- lar matrix group. The effects were more pronounced in the ADSCs group than in the dADSCs group. These experimental results indicated that ADSCs transplantation could be an early interventional strategy to promote regeneration after recurrent laryngeal nerve injury.
基金supported by the Youth Scholars Program of Beijing Normal University (No. 31601868 to CX)National Natural Science Foundation of China (No. 31301888 to DL)+1 种基金General scientific research project of Education Department of Liaoning Province (L2015196 to DL)Open Fund of Ministry of Education Key Laboratory for Biodiversity Sciences and Ecological Engineering, Beijing Normal University (K1401 to DL)
文摘Background: Individuality in vocalizations may provide an effective tool for surveying populations of the Common Cuckoo(Cuculus canorus) but there remains few data on which technique to use to identify individuality. In this research, we compared the within-and between-individual variation in cuckoo calls using two different analytical methods, and discuss the feasibility of using call individuality to count male cuckoos within a population.Methods: We recorded vocalization from 13 males, and measured 15 spectro-temporal variables for each call. The majority of these call variables(n = 12) have greater variation between individuals than within individual. We first calculated the similarity(Pearson's R) for each paired calls in order to find a threshold that could distinguish calls emitted from the same or different males, and then counted the number of males based on this distinction. Second, we used the more widely accepted technique of discriminant function analysis(DFA) to identify individual male cuckoos, and compared the correct rate of classifying individuals between the two analytical methods.Results: Similarity of paired calls from the same male was significantly higher than from different males. Under a relatively broad threshold interval, we achieved a high(>90%) correct rate to distinguish calls and an accurate estimate of male numbers. Based on banded males(n = 3), we found the similarity of paired calls from different days was lower when compared with paired calls from the same day, but this change did not obscure individual identification, as similarity values of paired calls from different days were still larger than the threshold used to distinguish calls from the same or different males. DFA also yielded a high rate(91.9%) of correct classification of individuals.Conclusions: Our study suggests that identifying individual vocalizations can form the basis of an appropriate survey method for counting male cuckoos within a population, provided the performance of different analytical techniques are compared.
基金supported by the National Basic Research Program of China(No.2007CB411606)
文摘Ultrasonic communication in vertebrates is attracting increasing research interest.To determine if ultrasonic vocalization is common in birds,we recorded their vocalizations with ultrasound detectors in the Dongzhai National Nature Reserve of Henan Province,China.We found varying degrees of high frequency components in the vocalizations of 14 species and in several of these species,the frequency of harmonics was up to the range of ultrasound.We suggest that more studies are required to determine whether the high frequency components in avian vocalizations have functions and what these functions are.In addition,the ability of birds to hear sounds in the high frequency range also requires re-examination.
文摘41 cases still showing various symptoms and signs after having been operated on for intralaryngeal conditions were treated with Yiqi Kaiyin Tang (益气开音汤) the vital energy replenishing and voice restoring decoction). The chief clinical manifestations were hoarseness of vioce, lack of strength for speech, feeling of dryness and pain in the throat with foreign body sensation or burning sensation, and aphonia. The treatment lasted 15-60 days, resulting in cure in 19 cases, improvement in 16 cases, and no response in 6 cases, making a total effectiveness rate of 85.4%.
