小儿伤科泡腾颗粒中维持骨重塑稳态的生物活性分子筛选分析

目的:筛选小儿伤科泡腾颗粒中维持骨细胞骨重塑稳态的潜在中药质量标志物(Q-Marker)。方法:采用超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF-MS)方法对不同提取基相、批次的小儿伤科泡腾颗粒常温提取液进行活性化学药物成分色谱、质谱解析。阳性对照采用伊班膦酸钠,绘制小儿伤科泡腾颗粒UPLC-Q-TOF-MS指纹图谱,结合SwissADME在线开源数据库对各成分主峰主要分子成分进行鉴定。筛选差异性分子图谱并与骨细胞Dickkopf相关蛋白1(DKK1)进行分子对接虚拟适配,以结合能<-15.0 kJ/mol为筛选条件,筛选小儿伤科泡腾颗粒治疗闭合性骨折的潜在Q-Marker。结果:对我院多批次小儿伤科泡腾颗粒原材料进行随机分布组合,共制备得到24批次小儿伤科泡腾颗粒样品,样品相似度分布区间0.901~0.976,有效成分具备一致性。小儿伤科泡腾颗粒总离子流图分析结果显示,共鉴定出18个共有峰,鉴别明确12种化合物:(+)-荷包牡丹碱、齐墩果酸、1182-94-1、赤霉素A4、白桦脂酸、植物甾醇、硫酸黄连碱、T25789、芍药新苷、二氢血根碱、人参皂苷Rh2、盐酸异黄连碱。其中,(+)-荷包牡丹碱、齐墩果酸、1182-94-1、硫酸黄连碱、T25789、芍药新苷、二氢血根碱、人参皂苷Rh2、盐酸异黄连碱9种化合物筛选作为小儿伤科泡腾颗粒的潜在Q-Marker。结论:(+)-荷包牡丹碱、齐墩果酸、1182-94-1、硫酸黄连碱、T25789、芍药新苷、二氢血根碱、人参皂苷Rh2、盐酸异黄连碱可能是小儿伤科泡腾颗粒维持骨细胞骨重塑稳态的主要活性成分,具备抑制DKK1活性并加速骨细胞重建的分子学潜能,可作为小儿伤科泡腾颗粒治疗闭合性骨折损伤的潜在Q-Marker。

Relative Molecular Mass Distribution of BG Resins

Benzoguanamine formaldehyde (BG F) resins are a class of amino resins, which are important cross linking agents for epoxy, alkyol and acrylic resins, etc. The cross linking performance is the best one when the polymerization degree is 2 4. This paper discusses the effects of the pH value for polycondensation and the formaldehyde to benzoguanamine mole ratio in a methanol system, and compares the relative molecular mass distribution using the Flory statistics method.

Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

The reactions of exo-cellulase （cellobiohydrolase, CBH） and endo-cellulase （endoglucanase, EG） were investigated by analyzing the insoluble residues of microcrystalline cellulose （MCC） and filter paper cellulose （FPC） during enzymatic hydrolysis. Molecular parameters including molecular weight and its distribution, degree of polymerization, and radii of gyration were measured by size exclusion chromatography coupled with multi-angle laser light scattering. No significant change in MCC chains was found during the whole reaction period, indicating that CBH digestion follows a layer-by-layer solubilization manner. This reaction mode might be the major reason for slow enzymatic hydrolysis of cellulose. On the other hand, the degree of polymerization of FPC chains decreases rapidly in the initial reaction, indicating that EG digestion follows a random scission manner, which may create new ends for CBH easily. The slopes of the conformation plots for MCC and FPC increase gradually, indicating stronger chain stiffness of cellulose during hvdrolvsis

Effects of organic acids on heavy metal release or immobilization in contaminated soil

In order to explicit the environmental activity of heavy metals affected by different organic acids in soil,a batch incubation experiment was explored to investigate the influence of high relative molecular mass organic acid(HMWOA)(humic acid and fulvic acid)and low relative molecular mass organic acid(LMWOA)(threonic acid and oxalic acid)on the release or immobilization of Pb,Cu and Cd in soils.Results showed that LMWOA,especially threonic acid,had a good performance in the release of Pb,Cu and Cd from soils,and decrease in the fractions of HOAc-extractable,reducible and oxidable Pb,Cu and Cd.Conversely,HMWOA,especially humic acid,decreased the release of Pb,Cu and Cd,while it increased the fractions of HOAc-extractable,reducible and oxidable Pb,Cu and Cd,indicating that HMWOA can immobilize heavy metals.The release of Pb,Cu and Cd caused by LMWOA was attributed to the dissociation of soil organic matter and amorphous iron oxides since the total organic carbon and the water-soluble iron increased.The immobilization of Pb,Cu and Cd by HMWOA was attributed to the adsorption onto HMWOA followed by amorphous iron since HMWOA resulted in a significant decrease of zeta potential and an increase of amorphous iron oxide.It can be concluded that LMWOA has a potential application in soil washing remediation,while HMWOA can be used in the immobilization remediation for heavy metals contaminated soils.

Effect of Xenon Arc Lamp Irradiation on Properties of Polymethyl Methacrylate for Aviation

YB-2 aviation polymethyl methacrylate (PMMA) is irradiated in a xenon arc lamp weather resistance test chamber for 1620 hours. The tensile strength, light transmittance, surface morphology, relative molecular mass, infrared absorption spectrum and glass transition temperature (Tg) of PMMA exposed in xenon arc lamp for different durations are tested and characterized by universal testing machine, optical haze instrument, scanning electronic microscopy (SEM), gel permeation chromatograph (GPC), fourier transform infrared spectrometer (FT-IR) and differential scanning calorimetry (DSC), so as to comprehensively analyze the influence of xenon arc lamp irradiation on the performance of PMMA. The results reveal that under the effect of 1620 hours xenon arc lamp irradiation and periodic spraying water, the light transmittance and glass transition temperature do not change significantly, and no new chemical group is produced. After irradiated 360 hours, tiny cracks occur in the surface of PMMA, indicating that they occur at a certain degree of degradation, meanwhile, the main chain may be broken and the relative molecular mass of surface of the material decreases. After exposure of 720 hours, the tensile strength decreases about 30%.

高效凝胶渗透色谱法测定邻苯二甲酸羟丙甲基纤维素酯的相对分子质量及其分布

目的:建立一种邻苯二甲酸羟丙甲基纤维素酯的相对分子质量的质控方法。方法:采用高效凝胶渗透色谱法(HPG-PC),色谱柱为Styrage(Waters-HR4(7.8mm×300mm),以已知分子量的聚苯乙烯为标样,四氢呋喃为流动相;柱温40℃;流速1.0mL.min-1;示差折光检测器。结果:利用GPC软件处理得到邻苯二甲酸羟丙甲基纤维素酯的重均分子量,分子量分布和分布系数。结论:本文所建立HPGPC方法简便快速,重复性好(RSD<2%),适合于该类药物的质量控制。

HPLC法测定注射用重组人白介素-11中的相关蛋白

目的:建立测定注射用重组人白介素-11中相关蛋白的高效液相色谱法,对主要杂质进行鉴别。方法:采用HPLC法分离分析注射用重组人白介素-11相关蛋白,利用Agilent 1200型高效液相色谱仪,使用Sepax Bio-C4色谱柱(300?,250 mm×4.6 mm,5μm),以0.1%三氟醋酸水溶液为流动相A,70%乙腈溶液(含0.075%三氟醋酸)为流动相B,进行梯度洗脱,流速1.0 mL·min-1,检测波长220 nm,柱温40℃;采用UPLC-QTOF法分析重组人白介素-11相关蛋白,使用Waters BEH-C4色谱柱(300?,2.1 mm×100 mm,1.7μm),以0.1%甲酸水溶液为流动相A,0.1%甲酸乙腈溶液为流动相B,进行梯度洗脱,对主要相关蛋白进行鉴定。结果:UPLC-QTOF鉴定了3个主要相关蛋白,杂质1、2和3,相对分子质量分别为5 488.446、4 576.214和14 489.558。建立的HPLC测定相关蛋白方法具有良好的结果,与《中华人民共和国药典》2015年版三部中重组人白介素-11原液的纯度方法(HPLC法)比较,杂质检出能力更强。结论:建立的HPLC法简便,灵敏度高,专属性好,能有效反应药品质量。