Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and pyroptosis.The silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and inflammation.AIM To investigate the association between ferroptosis and pyroptosis and the upstream regulatory mechanisms.METHODS This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.C57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galactosamine(D-GalN)to induce ALF.Gasdermin D(GSDMD)^(-/-)mice were used as an experimental group.Histological changes in liver tissue were monitored by hematoxylin and eosin staining.ALT,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial kits.Ferroptosis-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain reaction.SIRT1,p53,and GSDMD were assessed by immunofluorescence analysis.RESULTS Serum AST and ALT levels were elevated in patients with ALF.SIRT1,solute carrier family 7a member 11(SLC7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-CoA synthetase long-chain family member 4(ACSL4)protein levels were elevated in human ALF liver tissue.In the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour necrosis factor alpha,IL-6,IL-2 and C-C motif ligand 2 levels were decreased and hepatic impairment was mitigated.In mice with GSDMD knockout,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLC7A11,elevation of ACSL4,and iron accumulation)were detected.In vitro,knockdown of p53 and overexpression of GPX4 reduced AST and ALT levels,the cytostatic rate,and GSDMD expression,restoring SLC7A11 depletion.Moreover,SIRT1 agonist and overexpression of SIRT1 alleviated acute liver injury and decreased iron deposition compared with results in the model group,accompanied by reduced p53,GSDMD,and ACSL4,and increased SLC7A11 and GPX4.Inactivation of SIRT1 exacerbated ferroptotic and pyroptotic cell death and aggravated liver injury in LPS/D-GalNinduced in vitro and in vivo models.CONCLUSION SIRT1 activation attenuates LPS/D-GalN-induced ferroptosis and pyroptosis by inhibiting the p53/GPX4/GSDMD signaling pathway in ALF.

Extended Lorenz majorization and frequencies of distances in an undirected network

Purpose:To contribute to the study of networks and graphs.Design/methodology/approach:We apply standard mathematical thinking.Findings:We show that the distance distribution in an undirected network Lorenz majorizes the one of a chain.As a consequence,the average and median distances in any such network are smaller than or equal to those of a chain.Research limitations:We restricted our investigations to undirected,unweighted networks.Practical implications:We are convinced that these results are useful in the study of small worlds and the so-called six degrees of separation property.Originality/value:To the best of our knowledge our research contains new network results,especially those related to frequencies of distances.

Cracking the silent gallstone code:Wait or operate?

The widespread availability of abdominal ultrasound has revealed the common occurrence of asymptomatic gallstones.While the treatment for symptomatic gallstones is clear,the benefits of minimally invasive laparoscopic cholecystectomy have sparked debate about the best approach to managing silent gallstones.The potential for asymptomatic gallstones to become symptomatic or lead to complications complicates the decision-making process regarding surgical intervention,as it's uncertain when or which patients might develop complications.Consequently,risk stratification appears to play a critical role in guiding decisions about silent gallstones.However,there is no definitive evidence to direct management,and a consensus-based on high-quality evidence is yet to be established.

Error Analysis of English Pronunciation Acquisition by Chinese Non-English Majors

Chinese non-English majors are a large group of English learners.In the process of English pronunciation acquisition,issues such as incomplete phonological knowledge,transfer of mother tongue,and overgeneralization,lead to confusion of phonemes and stress,misunderstanding of syllable structure,and errors of assimilation,drop,and epenthesis.The accuracy of English pronunciation can only be improved by knowing both English and Chinese phonological systems,strengthening the teaching of English phonological knowledge,and adopting various phonological training activities.

Usefulness of the Surgical Apgar Score to Predict the Occurrence of Major Complications in the Early Post-Operative Period of Major Surgeries: Experience of Two Second-Category Hospitals in Cameroon

Objective: The Surgical Apgar Score (SAS) is a tool for intraoperative stratification of the risk of serious complications in the early postoperative period. It varies from 0 to 10 points divided into three risk categories (0 to 4 high, 5 to 7 moderate, 8 to 10 low). The aim of the study was to evaluate its relevance in predicting the appearance of these complications. Material and methods: This descriptive and analytical study was carried out at the “Laquintinie” Hospital in Douala and at the Central Hospital in Yaounde, Cameroon. The main data were collected on a population of patients over 18 years old and recorded on a survey form. They consisted of variables of main interest and exposure variables. Univariate and multivariate statistical analysis using top-down logistic regression models made it possible to evaluate the association of each variable of main interest and each exposure variable. The association was significant at P Results: Of the 88 patients studied, the SAS was 3 hours. In multivariate, this link persisted only and strongly for the SAS OR (IC) 0.1 (0.1 - 0.2) and p = 000. Conclusion: The study found a specific and powerful link between the SAS score < 4 and the occurrence of complications in the early postoperative period, in favor of its relevance in predicting them.

Mobility of Major and Trace Elements during the Bauxitization Processes in Ngaoundal Area (Adamawa Cameroon): Implication on Mining Perspectives

This study was focused to assess major and trace elements in bauxitic duricrusts from Ngaoundal and its surroundings in order to establish their mining interest. To this end, fieldworks, mineralogical and geochemical analyses were carried out. Four facies of duricrust were identified and characterized from the summit to the top of the slope of the Ngaoundal mountain: scoriaceous, pisolitic, nodular and massive. Mineralogical and geochemical analyses performed on 16 samples, revealed a significant concentration of Al2O3 mainly in the scoriaceous facies (over 45% in grade), moderate in Fe2O3 (averaging 23.69%) and SiO2 (averaging 21.7%). Trace elements were generally low, excluding Cr (421 ppm on average), Zr (327 ppm on average and V (213 ppm on average). In addition, the limited quantities of alkalis (Na2O, K2O) and alkaline earths metals (MgO, CaO) coupled with the very high values of Chemical Index of Alteration (CIA) and Mineralogical Index of Alteration (MIA), (more than 99%) attest to the intense weathering of the studied materials. Allitization and monosiallitization constituted the crystallochemical phenomena that have led to the development of bauxitic minerals. More than 90% of gibbsite in scoriaceous facies, 52.21% - 76.01% of kaolinite in pisolitic facies and more than 40% of hematite in nodular facies were quantified. The relationships between the constitutive components indicated their interdependency during the bauxitization phenomenon. The mineralogical and geochemical properties highlighted the mining interest of the studied duricrusts to be valorized.

少年儿童翻译文学的译本风格计量研究——以Silent Spring三译本为例

本研究基于自建Silent Spring(《寂静的春天》)英汉平行语料库,以教育部编义务教育语文教材中的科普文章为标准参照,从可读性、趣味性和具象性三个维度,探讨少年儿童文学翻译文本风格特征及其与目标读者认知水平和接受能力的契合度,希冀能拓宽少年儿童翻译文学风格计量研究视野,为少年儿童文学的翻译传播研究提供参考。

基于QCA的五输入Majority门设计及应用

Majority门作为多数逻辑电路的基本逻辑单元,其性能直接影响整体电路的质量.使用量子元胞自动机(QCA)设计Majority门具有结构简单的优点.本文提出了一种三层电路实现五输入Majority门的设计,并以此设计了全加器,进一步应用于多位加法器和乘法器中,与已发表的电路设计比较表明,其版图使用面积和元胞数有明显的减少,加法器元胞数和面积改进最高可达43%和87. 2%,乘法器元胞数和面积改进最高可达48. 2%和100%.

基于CNFET的三输入Majority门电路设计

Majority(MAJ)运算和反相(INV)运算组成完备集,数字逻辑电路可以用基于"MAJ/INV"的MI(Majority-Inverter)逻辑来实现。三输入MAJ门是MI逻辑电路的一种基本门电路单元。本文设计了一种基于碳纳米管场效应晶体管(Carbon Nanotube Field Effect Transistor,CNFET)的三输入MAJ门电路,并用所设计的MAJ门实现三个多输入组合逻辑电路。实验结果表明,在采用相同的器件和工艺的条件下,与现有的设计相比,所设计的MAJ门在功耗和功耗延时积上的改进最高分别达到32.5%和45.3%。

基于Majority逻辑门映射的电路面积优化

提出了一种采用三输入Majority逻辑门(M门)和反相器实现电路逻辑功能的电路面积优化算法.该算法首先用动态规划策略完成电路的AIG(And-Inverter Graph)切割,然后通过搜索适合M门覆盖的AIG子结构以及与门和M门之间的等效替代,将AIG转换为适合M门映射的MIG(Majority-Inverter Graph),最后用Ω法则和Ψ法则对MIG进行简化,得到节点数更少的MIG,从而实现基于M门映射的电路面积优化.优化算法用C语言实现,并用MCNC电路测试.结果表明,相比于逻辑综合工具ABC的结果,用M门映射后电路面积可得到平均约12%左右的优化.

Lower Bounds on the Majority Domination Number of Graphs

Let G=(V,E) be a simple graph. For any real valued function f∶V→R and SV, let f(S)=∑ u∈S?f(u). A majority dominating function is a function f∶V→{-1,1} such that f(N)≥1 for at least half the vertices v∈V. Then majority domination number of a graph G is γ maj(G)=min{f(V)|f is a majority dominating function on G}. We obtain lower bounds on this parameter and generalize some results of Henning.

Coalition Formation in Weighted Simple-majority Games under Proportional Payoff Allocation Rules

In this paper, we introduce a simple coalition formation game in the environment of bidding, which is a special case of the weighted majority game （WMG）, and is named the weighted simple-majority game （WSMG）. In WSMG, payoff is allocated to the winners proportional to the players powers, which can be measured in various ways. We define a new kind of stability： the counteraction-stability （C-stability）, where any potential deviating players will confront counteractions of the other players. We show that C-stable coalition structures in WSMG always contains a minimal winning coalition of minimum total power. For the variant where powers are measured directly by their weights, we show that it is NP-hard to find a C-stable coalition structure and design a pseudo-polynomial time algorithm. Sensitivity analysis for this variant, which shows many interesting properties, is also done. We also prove that it is NP-hard to compute the Holler-Packel indices in WSMGs, and hence in WMGs as well.

A Comparative Study of Majority/Minority Logic Circuit Synthesis Methods for Post-CMOS Nanotechnologies

The physical limitations of complementary metal-oxide semiconductor?(CMOS) technology have led many researchers to consider other alternative technologies. Quantum-dot cellular automate (QCA), single electron tunneling (SET), tunneling phase logic (TPL), spintronic devices, etc., are some of the nanotechnologies that are being considered as possible replacements for CMOS. In these nanotechnologies, the basic logic units used to implement circuits are majority and/or minority gates. Several majority/minority logic circuit synthesis methods have been proposed. In this paper, we give a comparative study of the existing majority/minority logic circuit synthesis methods that are capable of synthesizing multi-input multi-output Boolean functions. Each of these methods is discussed in detail. The optimization priorities given to different factors such as gates, levels, inverters, etc., vary with technologies. Based on these optimization factors, the results obtained from different synthesis methods are compared. The paper also analyzes the optimization capabilities of different methods and discusses directions for future research in the synthesis of majority/minority logic networks.

Improved SMB speckle filtering of polarimetric SAR data with synergistic use of orientation angle compensation and spatial majority rule

The scattering-model-based(SMB)speckle filtering for polarimetric SAR(Pol SAR)data is reasonably effective in preserving dominant scattering mechanisms.However,the efficiency strongly depends on the accuracies of both the decomposition and classification of the scattering properties.In addition,a relatively weak speckle reduction particularly in distributed media was reported in the related literatures.In this work,an improved SMB filtering strategy is proposed considering the aforementioned deficiencies.First,the orientation angle compensation is incorporated into the SMB filtering process to remedy the overestimation of the volume scattering contribution in the Freeman-Durden decomposition.In addition,an algorithm to select the homogenous pixels is developed based on the spatial majority rule for adaptive speckle reduction.We demonstrate the superiority of the proposed methods in terms of scattering property preservation and speckle noise reduction using L-band Pol SAR data sets of San Francisco that were acquired by the NASA/JPL airborne SAR(AIRSAR)system.

What Makes a Good Second Language Learner from the Point of Majority Language Contexts

The reason to choose this topic is because among three types of context in natural second language learning settings,majority language contexts are undoubtedly the most important type to help L2 learners enhance their proficiencies of target languages in an effective way.The first part of the paper is review of the researches that have been conducted concerning majority language contexts.From following chapters,analysis will be made on the deficiencies of L2 learning and teaching in China as well as necessities of majority language contexts.Finally,basic strategies will be proposed to help L2 learners overcome various problems in majority language contexts.

基于majority和median表决算法的混合表决算法

本文提出结合majority和median的表决算法，问题得到较好改善：首先使用majority表决算法进行工作，当出现无法表决的情况时，计算数据分散程度，如果小于某个设定安全阈值，则使用median表决算法来处理这种情况，若大于安全阈值，则输出一个无法表决的信号。最终的仿真测试表明：结合majority和median表决系统是一种可行的方法，能够提高表决系统输出正确率；错误率虽然有小幅增加，但能够有效改善majority无法输出的情况，同时正确率有一定程度的提高。本文提出的表决算法可以适用于需要提高输出正确率并且可以适当降低安全性的应用环境，测试结果表明对于连续信号和离散信号都有较好的效果。

高校英语专业综合英语课程思政的教学设计研究——以“Silent Spring”一课的教学为例

课程思政的全面实施是新时代中国教育落实立德树人根本任务的迫切需求。综合英语课程作为高校英语专业的一门基础必修课,承担着重要的育人任务。在重新审视教学大纲和教学内容的前提下,本文对《现代大学英语精读3》(第二版)教材中“Silent Spring”一文思政元素融入的教学设计进行探讨和反思,以期在方法和模式上为相关课程实施课程思政提供启示和借鉴。

Reversible Digital Image Watermarking Scheme Using Bit Replacement and Majority Algorithm Technique

The current paper presents a new digital watermarking method through bit replacement technology, which stores mul-tiple copies of the same data that is to be hidden in a scrambled form in the cover image. In this paper an indigenous approach is described for recovering the data from the damaged copies of the data under attack by applying a majority algorithm to find the closest twin of the embedded information. A new type of non-oblivious detection method is also proposed. The improvement in performance is supported through experimental results which show much enhancement in the visual and statistical invisibility of hidden data.

Majority Coloring of r-Regular Digraph

A majority k-coloring of a digraph D with k colors is an assignment c:V(D)→{1,2,…,k},such that for every v∈V(D),we have c(w)=c(v)for at most half of all out-neighbors w∈N^(+)(v).For a natural number k≥2,a 1/k-majority coloring of a digraph is a coloring of the vertices such that each vertex receives the same color as at most a 1/k proportion of its out-neighbours.Kreutzer,Oum,Seymour,van der Zypen and Wood proved that every digraph has a majority 4-coloring and conjectured that every digraph admits a majority 3-coloring.Gireao,Kittipassorn and Popielarz proved that every digraph has a 1/k-majority 2k-coloring and conjectured that every digraph admits a 1/k majority(2k-1)-coloring.We showed that every r-regular digraph D with r>36ln(2n)has a majority 3-coloring and proved that every digraph D with minimum outdegreeδ+>2k2(2k-1)/(k-1)^(2)ln2(n)[(2k-1)n]has a 1/k-majority(2k-1)-coloring.We showed that every r-regular digraph D with r>36ln(2n)has a majority 3-coloring and proved that every digraph D with minimum outdegreeδ+>,2k^(2)(2k-1)^(2)/(k-1)^(2)ln[(2k-1)n]has a 1/k-majority(2k-1)-coloring.And we also proved that every r-regular digraph D with r>3k^(2)(2k-1)/(k-1)^2ln(2n)has a 1/k-majority(2k-1)-coloring.

Protective Effect of Silent Mating Type Information Regulation 2 Homolog 1 on TGF-β1 Pathway via mTOR in Diabetic Nephropathy

Objective: To demonstrate whether the expression of silent mating type information regulation 2 homolog 1 (SIRT1) affects the level of TGF-β1 and Smad3 in HEK293 cells through regulating mTOR. Methods: First, recombinant plasmids DNA (rSIRT1) and siRNA targeting SIRT1 were constructed which were transfected into Human Embryonic Kidney 293 cell (HEK293) cells, respectively. Then, the generation of intracellular ROS in cells was examined by flow cytometry using the oxidation-sensitive probe. Last, the expressions of TGF-β1, smad3, P53, mTOR, p-mTOR, LC3-I and LC3-II in cells were detected to observe the effect of SIRT1 on TGF-β1 Pathway by western blot analysis. Results: We demonstrated that overexpressing of SIRT1 may decrease TGF-β1 and Smad3 expression in HEK293 cells through regulating mTOR. In addition, the result is the opposite when SIRT1 was silent in HEK293 cells. Conclusions: SIRT1 is closely related to TGF-β1/Smad3 pathway that correlates with the regulation of mTOR and ROS generation and causes diabetic nephropathy. The available evidence implies that SIRT1 has great potential as a clinical target for the prevention and treatment of renal fibrosis in the development of DN.