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Functional analysis of the methyltransferase SMYD in the single-cell model organism Tetrahymena thermophila 被引量:2
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作者 Xiaolu Zhao Yuan Li +6 位作者 Lili Duan Xiao Chen Fengbiao Mao Mina Juma Yifan Liu Weibo Song Shan Gao 《Marine Life Science & Technology》 2020年第2期109-122,共14页
Lysine methylation of histones and non-histones plays a pivotal role in diverse cellular processes.The SMYD(SET and MYND domain)family methyltransferases can methylate various histone and non-histone substrates in mam... Lysine methylation of histones and non-histones plays a pivotal role in diverse cellular processes.The SMYD(SET and MYND domain)family methyltransferases can methylate various histone and non-histone substrates in mammalian systems,implicated in HSP90 methylation,myofilament organization,cancer inhibition,and gene transcription regulation.To resolve controversies concerning SMYD's substrates and functions,we studied SMYD1(TTHERM_00578660),the only homologue of SMYD in the unicellular eukaryote Tetrahymena thermophila.We epitope-tagged SMYD1,and analyzed its localization and interactome.We also characterized △SMYD1 cells,focusing on the replication and transcription phenotype.Our results show that:(1)SMYD1 is present in both cytoplasm and transcriptionally active macronucleus and shuttles between cytoplasm and macronucleus,suggesting its potential association with both histone and non-histone substrates;(2)SMYD1 is involved in DNA replication and regulates transcription of metabolism-related genes;(3)HSP90 is a potential substrate for SMYD1 and it may regulate target selection of HSP90,leading to pleiotropic effects in both the cytoplasm and the nucleus. 展开更多
关键词 Tetrahymena thennophila METHYLTRANSFERASE SMYD DNA replication TRANSCRIPTION HSP90
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