Two new complexes： [Cu（TBZ）（bipy）Cl]Cl[H2O （1） and [Cu（TBZ）（phen）CI]CloH20 （2） [TBZ=2-（4＇-thiazolyl）- benzimidazole, phen = 1,10-phenanthroline and bipy =2,2＇-bipyridine] have been synthesized and cha...Two new complexes： [Cu（TBZ）（bipy）Cl]Cl[H2O （1） and [Cu（TBZ）（phen）CI]CloH20 （2） [TBZ=2-（4＇-thiazolyl）- benzimidazole, phen = 1,10-phenanthroline and bipy =2,2＇-bipyridine] have been synthesized and characterized by elemental analysis, molar conductivity, IR, and UV-vis methods. Complex 2, structurally characterized by single-crystal X-ray crystallography, crystallizes in the monoclinic space group P21/c in a unit cell of a = 0.85257（12） nm, b=2.5358（4） nm, c=1.15151（13） nm, β=118.721（8）°, V=2.183.2（5） nm^3, Z=4, Dc=1.624 gocm 3, μ= 1.367 mm^-1. The complexes, free ligands and chloride copper（II） salt were each tested for their ability to inhibit the growth of two gram-positive （B. subtilis and S. aureus） and two gram-negative （Salmonella and E. coli） bacteria. The complexes showed good antibacterial activities against the microorganisms. The interaction between the com- plexes and calf thymus DNA in aqueous solution was investigated adopting electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and cyclic voltammetry. Results suggest that the two complexes can bind to DNA by intercalative mode. In addition, the result of agarose gel electrophoresis suggested that the complexes can cleave the plasmid DNA at physiological pH and room temperature. Mechanistic studies with different inhibiting reagents reveal that hydroxyl radicals, and a singlet oxygen-like copper-oxo species are all involved in the DNA scission process mediated by the complexes.展开更多
The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 ad...The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 adducts have an excellent efficiency in eliminating superoxide radical generated from auto oxidation of pyrogallol. At a mass concentration level of 150 μg/mL for β alanine C 60 adducts, the radical scavenging efficiency is approximately equivalent to one enzyme unit, in vitro experiments show that β alanine C 60 adducts can enhance the activity of mouse thymus cells and prolong their life time span. It reveals the potential use of these β alanine C 60 adducts as the novel potent free radical scavengers in biological systems.展开更多
文摘Two new complexes： [Cu（TBZ）（bipy）Cl]Cl[H2O （1） and [Cu（TBZ）（phen）CI]CloH20 （2） [TBZ=2-（4＇-thiazolyl）- benzimidazole, phen = 1,10-phenanthroline and bipy =2,2＇-bipyridine] have been synthesized and characterized by elemental analysis, molar conductivity, IR, and UV-vis methods. Complex 2, structurally characterized by single-crystal X-ray crystallography, crystallizes in the monoclinic space group P21/c in a unit cell of a = 0.85257（12） nm, b=2.5358（4） nm, c=1.15151（13） nm, β=118.721（8）°, V=2.183.2（5） nm^3, Z=4, Dc=1.624 gocm 3, μ= 1.367 mm^-1. The complexes, free ligands and chloride copper（II） salt were each tested for their ability to inhibit the growth of two gram-positive （B. subtilis and S. aureus） and two gram-negative （Salmonella and E. coli） bacteria. The complexes showed good antibacterial activities against the microorganisms. The interaction between the com- plexes and calf thymus DNA in aqueous solution was investigated adopting electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and cyclic voltammetry. Results suggest that the two complexes can bind to DNA by intercalative mode. In addition, the result of agarose gel electrophoresis suggested that the complexes can cleave the plasmid DNA at physiological pH and room temperature. Mechanistic studies with different inhibiting reagents reveal that hydroxyl radicals, and a singlet oxygen-like copper-oxo species are all involved in the DNA scission process mediated by the complexes.
文摘The ability of water soluble β alanine C 60 adducts for superoxide anion radical scavenging has been studied by spectrophotometry and the modified pyrogallol auto oxidation method. It is found that β alanine C 60 adducts have an excellent efficiency in eliminating superoxide radical generated from auto oxidation of pyrogallol. At a mass concentration level of 150 μg/mL for β alanine C 60 adducts, the radical scavenging efficiency is approximately equivalent to one enzyme unit, in vitro experiments show that β alanine C 60 adducts can enhance the activity of mouse thymus cells and prolong their life time span. It reveals the potential use of these β alanine C 60 adducts as the novel potent free radical scavengers in biological systems.
