A protocol for micropropagation using nodal explants from mature Pinus massoniana trees has been developed. Time of explant collection is crucial for the initial success of aseptic culture. Explants collected in early...A protocol for micropropagation using nodal explants from mature Pinus massoniana trees has been developed. Time of explant collection is crucial for the initial success of aseptic culture. Explants collected in early March gave the highest percentage of explant survival(64.5%) and shoot-forming percentage(52.3%). Thidiazuron(TDZ) concentration significantly influenced shoot formation; 4 μM TDZ was optimum, with 4.8 shoots produced per explant with a mean length of 7.1 cm after 120 days of culture. Regenerated shoots rooted for 60 days in basic medium with 1μM NAA were ready for growth in pots. This is the first report on plantlet regeneration in vitro from mature trees of P. massoniana that provides a reliable method for propagating selected elites.展开更多
Populus species are important resources for industry and in scientific study on biological and agricultural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar(Populus ciliata...Populus species are important resources for industry and in scientific study on biological and agricultural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar(Populus ciliata wall. ex Royle). The effect of TDZ alone and in combination with adenine and NAA was studied on the regeneration potential of petiole explants. The explants were excised from Himalayan poplar plants grown in glasshouses. After surface sterilization the explants were cultured on shoot induction medium. High percentage shoot regeneration(86 %) was recorded on MS medium supplemented with 0.004 mg L^-1TDZ and 79.7 mg L^-1adenine. The regenerated shoots for elongation and multiplication were transferred to MS + 0.5 mg L^-1BAP + 0.2 mg L^-1IAA +0.3 mg L-1GA3. Root regeneration from shoots developed in vitro was observed on MS medium supplemented with 0.10 mg L^-1IBA. Himalayan poplar plantlets could be produced within2 months after acclimatization in a sterile mixture of sand and soil. We developed a high efficiency plant regeneration protocol from petiole explants of P. ciliata.展开更多
The effectiveness of thidiazuron (TDZ) on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid (NAA) or 2, 4- dichlorophenoxyacetic acid (2, 4-...The effectiveness of thidiazuron (TDZ) on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid (NAA) or 2, 4- dichlorophenoxyacetic acid (2, 4-D). With the increasing of TDZ level, the induction rate of adventitious buds appeared an increasing trend and the highest rate was obtained by the combination of 2 mg/L TDZ with 0.1 mg/L NAA.The excessive level of TDZ could induce useless cellae and easily produce hyperhydricity. Inhibition of shoot elongation by TDZ was overcome through transferring shoot cultures to a shoot proliferation medium lacking TDZ or containing NAA, benzyladenine (BA) and TDZ. Indole- 3-butyric acid (IBA) was used to stimulate the rooting in vitro. Based on the number and length of rooting, the treatment with 0.5 mg/L IBA was the best.展开更多
基金funded by the Science Research and Technology Development from the Department of Science and Technology of Guangxi(14125008-2-17,1598006-5-7)the Natural Science Foundation of China(31360178)+1 种基金the Key Program of Guangxi Forestry Bureau([2015]7)as an independent project by the Key Laboratory of Gaungxi Fine Timber Forest Resources Cultivation(13A-01-01)
文摘A protocol for micropropagation using nodal explants from mature Pinus massoniana trees has been developed. Time of explant collection is crucial for the initial success of aseptic culture. Explants collected in early March gave the highest percentage of explant survival(64.5%) and shoot-forming percentage(52.3%). Thidiazuron(TDZ) concentration significantly influenced shoot formation; 4 μM TDZ was optimum, with 4.8 shoots produced per explant with a mean length of 7.1 cm after 120 days of culture. Regenerated shoots rooted for 60 days in basic medium with 1μM NAA were ready for growth in pots. This is the first report on plantlet regeneration in vitro from mature trees of P. massoniana that provides a reliable method for propagating selected elites.
文摘Populus species are important resources for industry and in scientific study on biological and agricultural systems. Our objective was to enhance the frequency of plant regeneration in Himalayan poplar(Populus ciliata wall. ex Royle). The effect of TDZ alone and in combination with adenine and NAA was studied on the regeneration potential of petiole explants. The explants were excised from Himalayan poplar plants grown in glasshouses. After surface sterilization the explants were cultured on shoot induction medium. High percentage shoot regeneration(86 %) was recorded on MS medium supplemented with 0.004 mg L^-1TDZ and 79.7 mg L^-1adenine. The regenerated shoots for elongation and multiplication were transferred to MS + 0.5 mg L^-1BAP + 0.2 mg L^-1IAA +0.3 mg L-1GA3. Root regeneration from shoots developed in vitro was observed on MS medium supplemented with 0.10 mg L^-1IBA. Himalayan poplar plantlets could be produced within2 months after acclimatization in a sterile mixture of sand and soil. We developed a high efficiency plant regeneration protocol from petiole explants of P. ciliata.
基金Supported by project of the Science and technology Ministry of China (Grant No.02 EFN216700794)
文摘The effectiveness of thidiazuron (TDZ) on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid (NAA) or 2, 4- dichlorophenoxyacetic acid (2, 4-D). With the increasing of TDZ level, the induction rate of adventitious buds appeared an increasing trend and the highest rate was obtained by the combination of 2 mg/L TDZ with 0.1 mg/L NAA.The excessive level of TDZ could induce useless cellae and easily produce hyperhydricity. Inhibition of shoot elongation by TDZ was overcome through transferring shoot cultures to a shoot proliferation medium lacking TDZ or containing NAA, benzyladenine (BA) and TDZ. Indole- 3-butyric acid (IBA) was used to stimulate the rooting in vitro. Based on the number and length of rooting, the treatment with 0.5 mg/L IBA was the best.
