There are lots of researches on fixture layout optimization for large thin-walled parts.Current researches focus on the positioning problem,i.e.,optimizing the positions of a constant number of fixtures.However,how to...There are lots of researches on fixture layout optimization for large thin-walled parts.Current researches focus on the positioning problem,i.e.,optimizing the positions of a constant number of fixtures.However,how to determine the number of fixtures is ignored.In most cases,the number of fixtures located on large thin-walled parts is determined based on engineering experience,which leads to huge fixture number and extra waste.Therefore,this paper constructs an optimization model to minimize the number of fixtures.The constraints are set in the optimization model to ensure that the part deformation is within the surface profile tolerance.In addition,the assembly gap between two parts is also controlled.To conduct the optimization,this paper develops an improved particle swarm optimization(IPSO)algorithm by integrating the shrinkage factor and adaptive inertia weight.In the algorithm,particles are encoded according to the fixture position.Each dimension of the particle is assigned to a sub-region by constraining the optional position range of each fixture to improve the optimization efficiency.Finally,a case study on ship curved panel assembly is provided to prove that our method can optimize the number of fixtures while meeting the assembly quality requirements.This research proposes a method to optimize the number of fixtures,which can reduce the number of fixtures and achieve deformation control at the same time.展开更多
An increasing number of researchers have researched fixture layout optimization for thin-walled part assembly during the past decades.However,few papers systematically review these researches.By analyzing existing lit...An increasing number of researchers have researched fixture layout optimization for thin-walled part assembly during the past decades.However,few papers systematically review these researches.By analyzing existing literature,this paper summarizes the process of fixture layout optimization and the methods applied.The process of optimization is made up of optimization objective setting,assembly variation/deformation modeling,and fixture layout optimization.This paper makes a review of the fixture layout for thin-walled parts according to these three steps.First,two different kinds of optimization objectives are introduced.Researchers usually consider in-plane variations or out-of-plane deformations when designing objectives.Then,modeling methods for assembly variation and deformation are divided into two categories:Mechanism-based and data-based methods.Several common methods are discussed respectively.After that,optimization algorithms are reviewed systematically.There are two kinds of optimization algorithms:Traditional nonlinear programming and heuristic algorithms.Finally,discussions on the current situation are provided.The research direction of fixture layout optimization in the future is discussed from three aspects:Objective setting,improving modeling accuracy and optimization algorithms.Also,a new research point for fixture layout optimization is discussed.This paper systematically reviews the research on fixture layout optimization for thin-walled parts,and provides a reference for future research in this field.展开更多
Lightweight thin-walled structures with lattice infill are widely desired in satellite for their high stiffness-to-weight ratio and superior buckling strength resulting fromthe sandwich effect.Such structures can be f...Lightweight thin-walled structures with lattice infill are widely desired in satellite for their high stiffness-to-weight ratio and superior buckling strength resulting fromthe sandwich effect.Such structures can be fabricated bymetallic additive manufacturing technique,such as selective laser melting(SLM).However,the maximum dimensions of actual structures are usually in a sub-meter scale,which results in restrictions on their appliance in aerospace and other fields.In this work,a meter-scale thin-walled structure with lattice infill is designed for the fuel tank supporting component of the satellite by integrating a self-supporting lattice into the thickness optimization of the thin-wall.The designed structure is fabricated by SLM of AlSi10Mg and cold metal transfer welding technique.Quasi-static mechanical tests and vibration tests are both conducted to verify the mechanical strength of the designed large-scale lattice thin-walled structure.The experimental results indicate that themeter-scale thin-walled structure with lattice infill could meet the dimension and lightweight requirements of most spacecrafts.展开更多
Pitting corrosion is harmful during bridge construction,which will lead to uneven roughness of steel surfaces and reduce the thickness of steel.Hence,the effect of pitting corrosion on the mechanical properties of col...Pitting corrosion is harmful during bridge construction,which will lead to uneven roughness of steel surfaces and reduce the thickness of steel.Hence,the effect of pitting corrosion on the mechanical properties of cold-formed thin-walled steel stub columns is studied,and the empirical formulas are established through regression fitting to predict the ultimate load of web and flange under pitting corrosion.In detail,the failure modes and load-displacement curves of specimens with different locations,area ratios,and depths are obtained through a large number of non-linear finite element analysis.As for the specimens with pitting corrosion on the web,all the specimens are subject to local buckling failure,and the failure mode will not change with pitting corrosion,but the failure location will change with pitting corrosion location;the size,location,and area ratio of pitting corrosion have little influence on the ultimate load of cold-formed thin-walled steel short columns,but the loss rate of pitting corrosion section area has a greater impact on the ultimate bearing capacity.As for the specimen with flange pitting corrosion,the location and area ratio of pitting corrosion have less influence on the ultimate load of cold-formed thin-walled steel short columns,and the section area loss rate has greater influence on the ultimate bearing capacity;the impact of web pitting corrosion on the ultimate load is greater than that of flange pitting corrosion under the same condition of pitting corrosion section area.The prediction formulas of limit load which are suitable for pitting corrosion of web and flange are established,which can provide a reference for performance evaluation of corroded cold-formed thin-walled steel.展开更多
This paper develops a new numerical framework for modeⅢcrack problems of thin-walled structures by integrating multiple advanced techniques in the boundary element literature.The details of special crack-tip elements...This paper develops a new numerical framework for modeⅢcrack problems of thin-walled structures by integrating multiple advanced techniques in the boundary element literature.The details of special crack-tip elements for displacement and stress are derived.An exponential transformation technique is introduced to accurately calculate the nearly singular integral,which is the key task of the boundary element simulation of thin-walled structures.Three numerical experiments with different types of cracks are provided to verify the performance of the present numerical framework.Numerical results demonstrate that the present scheme is valid for modeⅢcrack problems of thin-walled structures with the thickness-to-length ratio in the microscale,even nanoscale,regime.展开更多
The application of continuous natural fibers as reinforcement in composite thin-walled structures offers a feasible approach to achieve light weight and high strength while remaining environmentally friendly.In additi...The application of continuous natural fibers as reinforcement in composite thin-walled structures offers a feasible approach to achieve light weight and high strength while remaining environmentally friendly.In addition,additive manufacturing technology provides a favorable process foundation for its realization.In this study,the printability and energy absorption properties of 3D printed continuous fiber reinforced thin-walled structures with different configurations were investigated.The results suggested that a low printing speed and a proper layer thickness would mitigate the printing defects within the structures.The printing geometry accuracy of the structures could be further improved by rounding the sharp corners with appropriate radii.This study successfully fabricated structures with vari-ous configurations characterized by high geometric accuracy through printing parameters optimization and path smoothing.Moreover,the compressive property and energy absorption characteristics of the structures under quasi-static axial compression were evaluated and compared.It was found that all studied thin-walled structures exhibited progressive folding deformation patterns during compression.In particular,energy absorption process was achieved through the combined damage modes of plastic deformation,fiber pullout and delamination.Furthermore,the com-parison results showed that the hexagonal structure exhibited the best energy absorption performance.The study revealed the structure-mechanical property relationship of 3D printed continuous fiber reinforced composite thin-walled structures through the analysis of multiscale failure characteristics and load response,which is valuable for broadening their applications.展开更多
Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSP...Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.展开更多
In machining processes,chatter vibrations are always regarded as one of the major limitations for production quality and efficiency.Accurate and timely monitoring of chatter is helpful to maintain stable machining ope...In machining processes,chatter vibrations are always regarded as one of the major limitations for production quality and efficiency.Accurate and timely monitoring of chatter is helpful to maintain stable machining operations.At present,most chatter monitoring methods are based on the energy level at specified chatter frequencies or frequency bands.However,the spectral features of chatter could change during machining operations due to complexity and time-varying dynamics of the physical machining process.The purpose of this paper is to investigate the time-varying chatter features in turning of thin-walled tubular workpieces from the perspective of entropy.The airborne acoustics was selected as the source of information for machining condition monitoring.First,corresponding to the distinguishing surface topographies relevant to machining conditions,the features of the sound signal emitted during turning of the thin-walled cylindrical workpieces were extracted using the spectral analysis and wavelet packet transform,respectively.It was shown that the dominant vibration frequency as well as the energy distribution could shift with the transition of the machining status.After that,two relative entropy indicators based on the spectrum and the wavelet packet energy were constructed to identify chattering events in turning of the thin-walled tubes.The experimental results demonstrate that the proposed indicators could accurately reflect the transition of machining conditions with high sensitivity and robustness in comparison with the traditional FFT-based methods.The achievement of this study lays the foundations of the online chatter monitoring and control technique for turning of the thin-walled tubular workpieces.展开更多
In tropical regions,heavy rainfall induces erosion and shallow landslides on road embankments.Cement-based stabilization methods,common in these regions,contribute to climate change due to their high carbon footprint....In tropical regions,heavy rainfall induces erosion and shallow landslides on road embankments.Cement-based stabilization methods,common in these regions,contribute to climate change due to their high carbon footprint.This study explored the potential application of coir fiber-reinforced laterite soil-bottom ash mixtures as embankment materials in the tropics.The objective is to enhance engineered embankment slopes'erosion resistance and stability while offering reuse options for industrial byproducts.This study examined various mix designs for unconfined compressive strength(UCS)and permeability,utilizing 30%bottom ash(BA)and 1%coir fiber(CF)with varying sizes ranging from 10 to 40 mm,6%lime,and laterite soil(LS),followed by microstructural analyses.The results demonstrate that the compressive strength increases as the CF length increases to 25 mm.In contrast,permeability increases continuously with increasing CF length.Lime-treated mixtures exhibit superior short-and long-term strength and reduce permeability owing to the formation of cementitious materials,as confirmed by microstructural analyses.A lab-scale slope box was constructed to evaluate the surface erosion of the stabilized laterite soil embankment.Based on the rainfall simulation results,the LS-BA-CF mixtures show better resistance to erosion and deformation compared to untreated LS,especially when lime is added to the top layer.This study provides insights into a sustainable and cost-effective approach for slope stabilization using BA and CF,offering a promising solution for tropical regions susceptible to surface erosion and landslides.展开更多
Lignin,lignosulfonate,and synthesized phosphorylated lignosulfonate were introduced as greenfillers in citric acid-sucrose adhesives for bonding particleboard fabricated from areca leaf sheath(ALS).The characteristics ...Lignin,lignosulfonate,and synthesized phosphorylated lignosulfonate were introduced as greenfillers in citric acid-sucrose adhesives for bonding particleboard fabricated from areca leaf sheath(ALS).The characteristics of particleboards were compared to that of ultralow emitting formaldehyde(ULEF-UF).Thefillers derived from Eucalyptus spp.kraft-lignin were added forflame retardancy enhancement.10%of each lignin and modified lig-nin was added into the ULEF-UF and citric acid-sucrose bonded particleboards.Analyses applied to particle-boards included thermal characteristics,X-ray diffraction analysis(XRD),morphological properties,Fourier transform infrared spectroscopy(FTIR),as well as physical,mechanical,andfire resistance characteristics of the laboratory-fabricated particleboards.Lignin and modified lignin resulted in improved thermal stability of the composites bonded with ULEF-UF while the improvement in the particleboard bonded with citric acid-sucrose was not significant.The introduction offiller exerted a higher influence on the UF-bonded particleboards compared to composites fabricated with citric acid-sucrose.Generally,the presence of lignin,lignosulfonate,and phosphorylated lignosulfonate enhanced the mechanical strength of the ULEF-bonded particleboards,although their dimensional stability has deteriorated.Markedly,the use of lignin and lignosulfonate enhanced thefire resis-tance of the particleboards produced with lower observed weight loss.All laboratory particleboards exhibited satisfactoryfire resistance,attaining a V-0 rating in according to the UL-94 standard.展开更多
Erectile dysfunction(ED)is one of the important complications of diabetes,which is very common in diabetic patients,affecting more than half of male patients,and the incidence of the disease is about 3.5 times that of...Erectile dysfunction(ED)is one of the important complications of diabetes,which is very common in diabetic patients,affecting more than half of male patients,and the incidence of the disease is about 3.5 times that of the normal population.The pathogenesis of diabetic erectile dysfunction(DMED)is complex,involving nerve,vascular,endocrine,muscular and psychological aspects.At present,the therapeutic approaches of DMED include drug therapy,surgery,physical therapy and so on.This article provides a review of current research on the pathogenesis and treatment of DMED.Further elucidation of the pathogenesis of DMED and the development of new therapeutic approaches are of great significance for the prevention and treatment of DMED.展开更多
Throwing out egg shells without using them depreciates a vital source of calcium. As an egg shell contains twice the amount of calcium a person needs a day, it is considered as the richest source of calcium of natural...Throwing out egg shells without using them depreciates a vital source of calcium. As an egg shell contains twice the amount of calcium a person needs a day, it is considered as the richest source of calcium of natural origin. Egg shells have been traditionally and widely used in medicine, beauty science and food production for decades. In spite of that, there is a lack of new solutions that profoundly study mineral elements and beneficial components contained in egg shells for further usage as a product. Assuming we consume 2 eggs a day, the yearly consumption for a person is 730 eggs. As beneficial component, the egg shell structure and mineral elements, were determined in 3 types of samples (Mongolian eggs, Russian eggs, and iodized eggs) by SEM-EDS (Scanning electron microscopy with energy dispersive spectroscopy), a state-of-the-art research method. Consequently, it was established that egg shells consists of Ca (72.6% - 85.7%), Mg (2.7% - 4.5%), Si (0.3% - 0.6%), P (7.0% - 18.1%), S (0.5% - 2.0%), K (0.4% - 0.9%), I (2.6% - 3.0%), respectively. Additionally, the D3.8 × 120, 250, 500, and 1000 times zoomed images of shell structure (SEM) of each sample were examined, and the results were compared and evaluated. The reverse titration method examination has demonstrated that the calcium carbonate (CaCO<sub>3</sub>) content in egg shells is 91% - 92.5%, and pH is 8.41 - 8.75. Ultimately, fertilizer containing 97.7% Ca without chemical additives has been extracted by grounding the eggshells to 4.4 μm, then preparing the mixture at the rate of 20:0.5 (shell: mix, enriched with mandarin and lemon peel) and adjusting its pH to 7.5 - 8.0. Further research on the impact of fertilizer on growing process of indoor flowers has been commenced.展开更多
Based on the theory of Timoshenko and thin-walled beams, a new finite element model of spatial thin-walled beams with general open cross sections is presented in the paper, in which several factors are included such a...Based on the theory of Timoshenko and thin-walled beams, a new finite element model of spatial thin-walled beams with general open cross sections is presented in the paper, in which several factors are included such as lateral shear deformation, warp generated by nonuni- form torsion and second-order shear stress, coupling of flexure and torsion, and large displacement with small strain. With an additional internal node in the element, the element stiffness matrix is deduced by incremental virtual work in updated Lagrangian (UL) formulation. Numerical examples demonstrate that the presented model well describes the geometrically nonlinear property of spatial thin-walled beams.展开更多
A new generation of fluid pressure forming technology has been developed for the three typical structures of tubes,sheets,and shells,and hard-to-deform material components that are urgently needed for aerospace,aircra...A new generation of fluid pressure forming technology has been developed for the three typical structures of tubes,sheets,and shells,and hard-to-deform material components that are urgently needed for aerospace,aircraft,automobile,and high-speed train industries.in this paper,an over all review is introduced on the state of the art in fundamentals and processes for lower-pressure hydroforming of tubular components,double-sided pressure hydroforming of sheet components,die-less hydroforming of ellipsoidai shells,and dual hardening hot medium forming af hard-to-deform materiais Particular attention is paid to deformation behavior,stress state adjustment,defect prevention,and typical applications.In addition,future development directions of fluid pressure forming technology are discussed,including hyper lower-loading forming for ultra-large non-uniform components,precision for ming for intermetallic compound and high-entropy alloy components,intelligent process and equipment,and precise finite element simulation of inhomogeneous and strong anisotropic thin shells.展开更多
基金Supported by National Natural Science Foundation of China(Grant No.52005371)Shanghai Pujiang Program of China(Grant No.2020PJD071)+1 种基金Shanghai Municipal Natural Science Foundation of China(Grant No.22ZR1463900)Fundamental Research Funds for the Central Universities of China.
文摘There are lots of researches on fixture layout optimization for large thin-walled parts.Current researches focus on the positioning problem,i.e.,optimizing the positions of a constant number of fixtures.However,how to determine the number of fixtures is ignored.In most cases,the number of fixtures located on large thin-walled parts is determined based on engineering experience,which leads to huge fixture number and extra waste.Therefore,this paper constructs an optimization model to minimize the number of fixtures.The constraints are set in the optimization model to ensure that the part deformation is within the surface profile tolerance.In addition,the assembly gap between two parts is also controlled.To conduct the optimization,this paper develops an improved particle swarm optimization(IPSO)algorithm by integrating the shrinkage factor and adaptive inertia weight.In the algorithm,particles are encoded according to the fixture position.Each dimension of the particle is assigned to a sub-region by constraining the optional position range of each fixture to improve the optimization efficiency.Finally,a case study on ship curved panel assembly is provided to prove that our method can optimize the number of fixtures while meeting the assembly quality requirements.This research proposes a method to optimize the number of fixtures,which can reduce the number of fixtures and achieve deformation control at the same time.
基金Supported by National Natural Science Foundation of China(Grant No.52005371)Shanghai Municipal Natural Science Foundation of China(Grant No.22ZR1463900)+1 种基金Fundamental Research Funds for the Central Universities of China(Grant No.22120220649)State Key Laboratory of Mechanical System and Vibration of China(Grant No.MSV202318).
文摘An increasing number of researchers have researched fixture layout optimization for thin-walled part assembly during the past decades.However,few papers systematically review these researches.By analyzing existing literature,this paper summarizes the process of fixture layout optimization and the methods applied.The process of optimization is made up of optimization objective setting,assembly variation/deformation modeling,and fixture layout optimization.This paper makes a review of the fixture layout for thin-walled parts according to these three steps.First,two different kinds of optimization objectives are introduced.Researchers usually consider in-plane variations or out-of-plane deformations when designing objectives.Then,modeling methods for assembly variation and deformation are divided into two categories:Mechanism-based and data-based methods.Several common methods are discussed respectively.After that,optimization algorithms are reviewed systematically.There are two kinds of optimization algorithms:Traditional nonlinear programming and heuristic algorithms.Finally,discussions on the current situation are provided.The research direction of fixture layout optimization in the future is discussed from three aspects:Objective setting,improving modeling accuracy and optimization algorithms.Also,a new research point for fixture layout optimization is discussed.This paper systematically reviews the research on fixture layout optimization for thin-walled parts,and provides a reference for future research in this field.
基金The authors are grateful for the support by National Key Research and Development Program of China(2021YFF0500300,2020YFB1708300)the National Natural Science Foundation of China(52205280,12172041).
文摘Lightweight thin-walled structures with lattice infill are widely desired in satellite for their high stiffness-to-weight ratio and superior buckling strength resulting fromthe sandwich effect.Such structures can be fabricated bymetallic additive manufacturing technique,such as selective laser melting(SLM).However,the maximum dimensions of actual structures are usually in a sub-meter scale,which results in restrictions on their appliance in aerospace and other fields.In this work,a meter-scale thin-walled structure with lattice infill is designed for the fuel tank supporting component of the satellite by integrating a self-supporting lattice into the thickness optimization of the thin-wall.The designed structure is fabricated by SLM of AlSi10Mg and cold metal transfer welding technique.Quasi-static mechanical tests and vibration tests are both conducted to verify the mechanical strength of the designed large-scale lattice thin-walled structure.The experimental results indicate that themeter-scale thin-walled structure with lattice infill could meet the dimension and lightweight requirements of most spacecrafts.
基金funded by the‘Research Project of the Sucheng to Sihong Section of the Yanluo Expressway-Measurement Technology and Application of Bridge Quality Project Based on UAV Binocular Imaging(No.00-00-JSFW-20230203-029)’,received by H.Z.Wang.
文摘Pitting corrosion is harmful during bridge construction,which will lead to uneven roughness of steel surfaces and reduce the thickness of steel.Hence,the effect of pitting corrosion on the mechanical properties of cold-formed thin-walled steel stub columns is studied,and the empirical formulas are established through regression fitting to predict the ultimate load of web and flange under pitting corrosion.In detail,the failure modes and load-displacement curves of specimens with different locations,area ratios,and depths are obtained through a large number of non-linear finite element analysis.As for the specimens with pitting corrosion on the web,all the specimens are subject to local buckling failure,and the failure mode will not change with pitting corrosion,but the failure location will change with pitting corrosion location;the size,location,and area ratio of pitting corrosion have little influence on the ultimate load of cold-formed thin-walled steel short columns,but the loss rate of pitting corrosion section area has a greater impact on the ultimate bearing capacity.As for the specimen with flange pitting corrosion,the location and area ratio of pitting corrosion have less influence on the ultimate load of cold-formed thin-walled steel short columns,and the section area loss rate has greater influence on the ultimate bearing capacity;the impact of web pitting corrosion on the ultimate load is greater than that of flange pitting corrosion under the same condition of pitting corrosion section area.The prediction formulas of limit load which are suitable for pitting corrosion of web and flange are established,which can provide a reference for performance evaluation of corroded cold-formed thin-walled steel.
基金supported by the National Natural Science Foundation of China(No.11802165)the China Postdoctoral Science Foundation(Grant No.2019M650158).
文摘This paper develops a new numerical framework for modeⅢcrack problems of thin-walled structures by integrating multiple advanced techniques in the boundary element literature.The details of special crack-tip elements for displacement and stress are derived.An exponential transformation technique is introduced to accurately calculate the nearly singular integral,which is the key task of the boundary element simulation of thin-walled structures.Three numerical experiments with different types of cracks are provided to verify the performance of the present numerical framework.Numerical results demonstrate that the present scheme is valid for modeⅢcrack problems of thin-walled structures with the thickness-to-length ratio in the microscale,even nanoscale,regime.
基金Supported by National Natural Science Foundation of China(Grant Nos.51905555,52105523)Hu-Xiang Youth Talent Program of China(Grant No.2020RC3009)Innovation-Driven Project of Central South University of China(Grant No.2019CX017).
文摘The application of continuous natural fibers as reinforcement in composite thin-walled structures offers a feasible approach to achieve light weight and high strength while remaining environmentally friendly.In addition,additive manufacturing technology provides a favorable process foundation for its realization.In this study,the printability and energy absorption properties of 3D printed continuous fiber reinforced thin-walled structures with different configurations were investigated.The results suggested that a low printing speed and a proper layer thickness would mitigate the printing defects within the structures.The printing geometry accuracy of the structures could be further improved by rounding the sharp corners with appropriate radii.This study successfully fabricated structures with vari-ous configurations characterized by high geometric accuracy through printing parameters optimization and path smoothing.Moreover,the compressive property and energy absorption characteristics of the structures under quasi-static axial compression were evaluated and compared.It was found that all studied thin-walled structures exhibited progressive folding deformation patterns during compression.In particular,energy absorption process was achieved through the combined damage modes of plastic deformation,fiber pullout and delamination.Furthermore,the com-parison results showed that the hexagonal structure exhibited the best energy absorption performance.The study revealed the structure-mechanical property relationship of 3D printed continuous fiber reinforced composite thin-walled structures through the analysis of multiscale failure characteristics and load response,which is valuable for broadening their applications.
基金supported by the Scientific and Innovative Action Plan of Shanghai(21N31900800)Shanghai Rising-Star Program(23QB1403500)+4 种基金the Shanghai Sailing Program(20YF1443000)Shanghai Science and Technology Commission,the Belt and Road Project(20310750500)Talent Project of SAAS(2023-2025)Runup Plan of SAAS(ZP22211)the SAAS Program for Excellent Research Team(2022(B-16))。
文摘Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.
基金The financial support of National Natural Science Foundation of China(Grant Nos.52175108,51805352)is gratefully acknowledgedWe also would like to acknowledge the Key Research and Development Project of Shanxi Province(Grant No.202102010101009).
文摘In machining processes,chatter vibrations are always regarded as one of the major limitations for production quality and efficiency.Accurate and timely monitoring of chatter is helpful to maintain stable machining operations.At present,most chatter monitoring methods are based on the energy level at specified chatter frequencies or frequency bands.However,the spectral features of chatter could change during machining operations due to complexity and time-varying dynamics of the physical machining process.The purpose of this paper is to investigate the time-varying chatter features in turning of thin-walled tubular workpieces from the perspective of entropy.The airborne acoustics was selected as the source of information for machining condition monitoring.First,corresponding to the distinguishing surface topographies relevant to machining conditions,the features of the sound signal emitted during turning of the thin-walled cylindrical workpieces were extracted using the spectral analysis and wavelet packet transform,respectively.It was shown that the dominant vibration frequency as well as the energy distribution could shift with the transition of the machining status.After that,two relative entropy indicators based on the spectrum and the wavelet packet energy were constructed to identify chattering events in turning of the thin-walled tubes.The experimental results demonstrate that the proposed indicators could accurately reflect the transition of machining conditions with high sensitivity and robustness in comparison with the traditional FFT-based methods.The achievement of this study lays the foundations of the online chatter monitoring and control technique for turning of the thin-walled tubular workpieces.
文摘In tropical regions,heavy rainfall induces erosion and shallow landslides on road embankments.Cement-based stabilization methods,common in these regions,contribute to climate change due to their high carbon footprint.This study explored the potential application of coir fiber-reinforced laterite soil-bottom ash mixtures as embankment materials in the tropics.The objective is to enhance engineered embankment slopes'erosion resistance and stability while offering reuse options for industrial byproducts.This study examined various mix designs for unconfined compressive strength(UCS)and permeability,utilizing 30%bottom ash(BA)and 1%coir fiber(CF)with varying sizes ranging from 10 to 40 mm,6%lime,and laterite soil(LS),followed by microstructural analyses.The results demonstrate that the compressive strength increases as the CF length increases to 25 mm.In contrast,permeability increases continuously with increasing CF length.Lime-treated mixtures exhibit superior short-and long-term strength and reduce permeability owing to the formation of cementitious materials,as confirmed by microstructural analyses.A lab-scale slope box was constructed to evaluate the surface erosion of the stabilized laterite soil embankment.Based on the rainfall simulation results,the LS-BA-CF mixtures show better resistance to erosion and deformation compared to untreated LS,especially when lime is added to the top layer.This study provides insights into a sustainable and cost-effective approach for slope stabilization using BA and CF,offering a promising solution for tropical regions susceptible to surface erosion and landslides.
基金funded by the Equity Project Universitas Sumatera Utara(Number:10/UN5.2.3.1/PPM/KPEP/2023),which is entitled Pengembangan Papan Partikel Tahan Api Rendah Emisi Berbahan Limbah Tanaman Mangrove dan Limbah Tanaman Pertanian Melalui Penambahan Lignin Terfosforilasi Sebagai Filler.PT Greenei Alam Indonesia(PT GAI)contributed to providing the areca leaf sheath through the implementation of a collaboration agreement with the Research Center for Biomass and Bioproducts BRIN FY 2023-2025.
文摘Lignin,lignosulfonate,and synthesized phosphorylated lignosulfonate were introduced as greenfillers in citric acid-sucrose adhesives for bonding particleboard fabricated from areca leaf sheath(ALS).The characteristics of particleboards were compared to that of ultralow emitting formaldehyde(ULEF-UF).Thefillers derived from Eucalyptus spp.kraft-lignin were added forflame retardancy enhancement.10%of each lignin and modified lig-nin was added into the ULEF-UF and citric acid-sucrose bonded particleboards.Analyses applied to particle-boards included thermal characteristics,X-ray diffraction analysis(XRD),morphological properties,Fourier transform infrared spectroscopy(FTIR),as well as physical,mechanical,andfire resistance characteristics of the laboratory-fabricated particleboards.Lignin and modified lignin resulted in improved thermal stability of the composites bonded with ULEF-UF while the improvement in the particleboard bonded with citric acid-sucrose was not significant.The introduction offiller exerted a higher influence on the UF-bonded particleboards compared to composites fabricated with citric acid-sucrose.Generally,the presence of lignin,lignosulfonate,and phosphorylated lignosulfonate enhanced the mechanical strength of the ULEF-bonded particleboards,although their dimensional stability has deteriorated.Markedly,the use of lignin and lignosulfonate enhanced thefire resis-tance of the particleboards produced with lower observed weight loss.All laboratory particleboards exhibited satisfactoryfire resistance,attaining a V-0 rating in according to the UL-94 standard.
基金supported by the Joint Project of Yunnan Provincial Science and Technology Department and Yunnan University of Chinese Medicine:Exploring the Mechanism of Yiqi Tongluo Decoction from Wa Ethnic Medicine in Regulating Autophagy to Relieve Diabetic Peripheral Neuropathy Based on the PI3K/mTOR Pathway(No.202101AZ070001-064).
文摘Erectile dysfunction(ED)is one of the important complications of diabetes,which is very common in diabetic patients,affecting more than half of male patients,and the incidence of the disease is about 3.5 times that of the normal population.The pathogenesis of diabetic erectile dysfunction(DMED)is complex,involving nerve,vascular,endocrine,muscular and psychological aspects.At present,the therapeutic approaches of DMED include drug therapy,surgery,physical therapy and so on.This article provides a review of current research on the pathogenesis and treatment of DMED.Further elucidation of the pathogenesis of DMED and the development of new therapeutic approaches are of great significance for the prevention and treatment of DMED.
文摘Throwing out egg shells without using them depreciates a vital source of calcium. As an egg shell contains twice the amount of calcium a person needs a day, it is considered as the richest source of calcium of natural origin. Egg shells have been traditionally and widely used in medicine, beauty science and food production for decades. In spite of that, there is a lack of new solutions that profoundly study mineral elements and beneficial components contained in egg shells for further usage as a product. Assuming we consume 2 eggs a day, the yearly consumption for a person is 730 eggs. As beneficial component, the egg shell structure and mineral elements, were determined in 3 types of samples (Mongolian eggs, Russian eggs, and iodized eggs) by SEM-EDS (Scanning electron microscopy with energy dispersive spectroscopy), a state-of-the-art research method. Consequently, it was established that egg shells consists of Ca (72.6% - 85.7%), Mg (2.7% - 4.5%), Si (0.3% - 0.6%), P (7.0% - 18.1%), S (0.5% - 2.0%), K (0.4% - 0.9%), I (2.6% - 3.0%), respectively. Additionally, the D3.8 × 120, 250, 500, and 1000 times zoomed images of shell structure (SEM) of each sample were examined, and the results were compared and evaluated. The reverse titration method examination has demonstrated that the calcium carbonate (CaCO<sub>3</sub>) content in egg shells is 91% - 92.5%, and pH is 8.41 - 8.75. Ultimately, fertilizer containing 97.7% Ca without chemical additives has been extracted by grounding the eggshells to 4.4 μm, then preparing the mixture at the rate of 20:0.5 (shell: mix, enriched with mandarin and lemon peel) and adjusting its pH to 7.5 - 8.0. Further research on the impact of fertilizer on growing process of indoor flowers has been commenced.
基金supported by the National Science Fund for Distinguished Young Scholars (No. 50725826).
文摘Based on the theory of Timoshenko and thin-walled beams, a new finite element model of spatial thin-walled beams with general open cross sections is presented in the paper, in which several factors are included such as lateral shear deformation, warp generated by nonuni- form torsion and second-order shear stress, coupling of flexure and torsion, and large displacement with small strain. With an additional internal node in the element, the element stiffness matrix is deduced by incremental virtual work in updated Lagrangian (UL) formulation. Numerical examples demonstrate that the presented model well describes the geometrically nonlinear property of spatial thin-walled beams.
基金supported by the National Science Fund for Distinguished Young Scholars(50525516)the National Natural Science Foundation of China(U1637209,51175111,50375036,and 59975021).
文摘A new generation of fluid pressure forming technology has been developed for the three typical structures of tubes,sheets,and shells,and hard-to-deform material components that are urgently needed for aerospace,aircraft,automobile,and high-speed train industries.in this paper,an over all review is introduced on the state of the art in fundamentals and processes for lower-pressure hydroforming of tubular components,double-sided pressure hydroforming of sheet components,die-less hydroforming of ellipsoidai shells,and dual hardening hot medium forming af hard-to-deform materiais Particular attention is paid to deformation behavior,stress state adjustment,defect prevention,and typical applications.In addition,future development directions of fluid pressure forming technology are discussed,including hyper lower-loading forming for ultra-large non-uniform components,precision for ming for intermetallic compound and high-entropy alloy components,intelligent process and equipment,and precise finite element simulation of inhomogeneous and strong anisotropic thin shells.