Sugar transporters(STs),which mainly mediate cellular sugar exchanges,play critical physiological roles in living organisms,and they may be responsible for sugar exchanges among various insect tissues.However,the mole...Sugar transporters(STs),which mainly mediate cellular sugar exchanges,play critical physiological roles in living organisms,and they may be responsible for sugar exchanges among various insect tissues.However,the molecular and physiological functions of insect STs are largely unknown.Here,16 STs of Helicoverpa armigera were identified.A phylogenetic analysis classified the putative HaSTs into 12 sub-families,and those identified in this study were distributed into 6 sub-families.Real-time polymerase chain reaction indicated that the 16 HaSTs had diverse tissue-specific expression levels.One transporter,HaST10,was highly expressed in thoracic muscles.A functional study using a Xenopus oocyte expression system revealed that HaST10 mediated both H+-driven trehalose and Na+-driven glucose antiport activities with high transport efficiency and low affinity levels.A HaST10 knockout clearly impaired the performance of H.armigera.Thus,HaST10 may participate in sugar-supply regulation and have essential physiological roles in H.armigera.展开更多
基金This work is supported by grants from National Natural Science Foundation of China(grant numbers 31601644 and 31970453)the State Key Laboratory of Integrated Management of Pest Insects and Rodents(grant numbers ChineselPM 1607 and ChineselPMl717).
文摘Sugar transporters(STs),which mainly mediate cellular sugar exchanges,play critical physiological roles in living organisms,and they may be responsible for sugar exchanges among various insect tissues.However,the molecular and physiological functions of insect STs are largely unknown.Here,16 STs of Helicoverpa armigera were identified.A phylogenetic analysis classified the putative HaSTs into 12 sub-families,and those identified in this study were distributed into 6 sub-families.Real-time polymerase chain reaction indicated that the 16 HaSTs had diverse tissue-specific expression levels.One transporter,HaST10,was highly expressed in thoracic muscles.A functional study using a Xenopus oocyte expression system revealed that HaST10 mediated both H+-driven trehalose and Na+-driven glucose antiport activities with high transport efficiency and low affinity levels.A HaST10 knockout clearly impaired the performance of H.armigera.Thus,HaST10 may participate in sugar-supply regulation and have essential physiological roles in H.armigera.
