Preparation and Evaluation of Novel Solid Chlorine Dioxide-based Disinfectant Powder in Single-pack

Objective To prepare and evaluate novel chlorine dioxide-based disinfectant powder in single-pack that is more convenient for use and transportation. Methods Orthogonal experiment was performed to determine the recipe of the disinfectant powder. Stability test, suspension quantitative bactericidal test, simulation field triM, and animal toxicity test were carded out to observe its bactericidal and toxicological effects. Results The orthogonal experiment showed that the type of water solution had no effect on the disinfectant powder and the best ratio of sodium chlorite to solid acid was 1：3. Ten grams of the disinfectant powder was fully dissolved in 20 mL water for 2 min, and diluted to 500 mL in water. After 5-10 min, the concentration of chlorine dioxide （CIO2） solution was 266 mg/L to 276 mg/L. After stored at 54℃ for 14 d, the average concentration of CIO2 was decreased by 5.03%. Suspension quantitative bactericidal test showed that the average killing logarithm （KL） value for both Staphylococcus aureus and Escherichia coli in 100 mg/L CIO2 solution for 2 min was over 5.00. In simulation field triM, the average descending KL value for Escherichia coli in the solution containing 100 mg/L CIO2 for 5 min was over 3.00. The mouse acute LD50 in the solution 5 times exceeded 5000 mg/kg. The disinfectant powder was not toxic and irritative to rabbit skin and had no mutagenic effect on mouse marrow polychromatic erythrocytes （PCE）. Conclusion The stability and bactericidal efficacy of solid chlorine dioxide-based disinfectant powder in single-pack are good. The solution containing 100 mg/L CIO2 can kill vegetative forms of bacteria. The concentration of CIO2 on the disinfecting surface of objects is 100 mg/L. The disinfectant powder is not toxic and irritative.

二氧化氯消毒粉的一元包装研究

目的研制使用和包装更为方便的一元包装ClO2消毒粉。方法设计正交试验优化消毒粉配方;按卫生部《消毒技术规范》2002年版要求进行了稳定性试验(加速试验法)、悬液定量杀菌试验、模拟现场试验以及毒性试验。结果正交试验确定配制水对消毒粉没有影响,且筛选出亚氯酸钠与固体酸的最佳配比为1∶3,取10g配制的消毒粉溶解到20ml水中,充分溶解,约2min后,再加水稀释到500ml,5～10min后可使ClO2质量浓度达到272mg/L;该消毒粉于54℃存放14d后,平均ClO2下降率是5.03%;含100mg/LClO2质量浓度的溶液对金黄色葡萄球菌和大肠埃希菌作用2min的平均杀灭对数值(KL)均>5.00;含100mg/LClO2质量浓度的溶液在模拟现场试验中对染于桌面的大肠埃希菌作用5min的KL>3.00。急性经口毒性试验合格,对家兔皮肤无刺激性,对小鼠骨髓嗜多染红细胞无致微核的作用。结论该一元包装ClO2消毒粉能达到理想的杀菌效果。

ClO_2消毒粉剂杀菌效果及毒性试验研究

目的研究三元一体包装ClO2消毒粉剂杀菌效果和毒性。方法采用悬液定量杀菌试验、能量试验和动物毒性试验进行了观察。结果以含25 mg/LClO2消毒液对悬液内大肠杆菌作用5 min,平均杀灭率为99.96%;12.5 mg/LClO2消毒液对金黄色葡萄球菌作用5 min,平均杀灭率为99.92%;50 mg/LClO2消毒液对枯草杆菌黑色变种芽孢作用10min,平均杀灭率为99.93%;含25%以上小牛血清对其杀灭大肠杆菌效果有轻度影响。以200mg/LClO2消毒液作用5 min,完全破坏悬液内HBsAg的抗原性。将三元一体包装ClO2消毒粉剂于54℃存放120 d,ClO2的下降率为8.2%。小鼠急性经口LD50值大于5000 mg/kg,以100 mg/LClO2消毒液对家兔皮肤刺激试验指数为0.63,属于轻度刺激性。结论三元一体包装ClO2消毒粉剂性能稳定,对细菌繁殖体和细菌芽孢具有良好的杀菌效果,属无毒、低刺激性消毒剂。

一种二氧化氯一元固体制剂消毒相关性能研究

目的研究一种二氧化氯一元粉剂的消毒相关性能。方法采用悬液定量杀菌试验和动物试验方法,对该消毒剂杀菌效果和毒性等进行了试验观察。结果用浓度500 mg/L该二氧化氯消毒剂溶液浸泡作用15 min,对悬液内大肠杆菌和金黄色葡萄球菌平均杀灭对数值>5.00。在相同浓度条件下浸泡作用30 min,对竹筷上大肠杆菌杀灭对数值>3.00;对木质物体表面自然菌杀灭对数值>1.00。该消毒剂急性经口毒性LD50>5 000 mg/kg(体重),小鼠骨髓嗜多染红细胞微核试验结果为阴性。结论本研究二氧化氯消毒粉剂具有较好的杀菌效果,属实际无毒级物质。