Three-dimensional circulation in northern South China Sea during early summer of 2015

Using the hydrographic data obtained during two nearly simultaneous surveys in June 2015,we carried out semi-diagnostic calculations with the help of a finite element model and a modified inverse method,to study the circulation in the northern South China Sea(NSCS)during the early summer of 2015.A number of new circulation features were found.(1)In most of the observation region,a large,basin-scale anticyclonic gyre appeared south of the 50-m isobath,which contained anticyclonic eddies.One anticyclonic eddy existed from the sea surface to 50-m depth,whose center showed no tilt,while the center of another eddy tilted eastward from the sea surface to 500-m depth.In the eastern part of the observation region,which is west of the Dongsha Islands,there was a sub-basin-scale cyclonic gyre containing a cyclonic eddy whose center tilted southward from the sea surface to 200-m depth.(2)There was a cross-continental slope current(CCSC)in the area southwest of the Dongsha Islands.Its volume transport was about 2.0×10^(6) m^(3)/s.(3)From the estimated order of magnitude of the stream function equation,the joint effect term of the baroclinity and relief(JEBAR)andβ-effect term are two important dynamic mechanisms affecting the variation of the circulation in the NSCS.(4)The JEBAR,as a transport-generating term,resulted in the dynamic mechanism determining the pattern of the depth-averaged flow across the contours of potential vorticity fH–1.Furthermore,we show that the negative values of the JEBAR were the most dominant dynamic mechanism,causing the CCSC southwest of the Dongsha Islands to deflect from the isobaths and veer toward the deep water.The CCSC around the Dongsha Islands was located further southwest during the early summer of 2015 than during the fall of 2005(revealed by a published study),which suggests that the location of the CCSC around the Dongsha Islands may vary with season.

Three-dimensional models:from cell culture to Patient-Derived Organoid and its application to future liposarcoma research

Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.

Three-dimensional line-of-sight-angle-constrained leader-following cooperative interception guidance law with prespecified impact time

To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D leader-following cooperative interception guidance law.First,in the LOS direction of the leader,an impact time-controlled guidance law is derived based on the fixed-time stability theory,which enables the leader to complete the interception task at a prespecified impact time.Next,in the LOS direction of the followers,by introducing a time consensus tracking error function,a fixed-time consensus tracking guidance law is investigated to guarantee the consensus tracking convergence of the time-to-go.Then,in the direction normal to the LOS,by combining the designed global integral sliding mode surface and the second-order Sliding Mode Control(SMC)theory,an innovative 3D LOS-angle-constrained interception guidance law is developed,which eliminates the reaching phase in the traditional sliding mode guidance laws and effectively saves energy consumption.Moreover,it effectively suppresses the chattering phenomenon while avoiding the singularity issue,and compensates for unknown interference caused by target maneuvering online,making it convenient for practical engineering applications.Finally,theoretical proof analysis and multiple sets of numerical simulation results verify the effectiveness,superiority,and robustness of the investigated guidance law.

The three-dimensional structure and seasonal variation of the North Pacific meridional overturning circulation

The three-dimensional structure and the seasonal variation of the North Pacific meridional overturning circulation （NPMOC） are analyzed based on the Simple Ocean Data Assimilation data and Argo profiling float data. The NPMOC displays a multi-cell structure with four cells in the North Pacific altogether. The TC and the STC are a strong clockwise meridional cell in the low latitude ocean and a weaker clockwise meridional cell between 7°N and 18°N, respectively, while the DTC and the subpolar cell are a weaker anticlockwise meridional cell between 3°N and 15°N and a weakest anticlockwise meridional cell between 35°N and 50°N, respectively. The DTC, the TC and the STC are all of very strong seasonal variations. As to the DTC, the southward transport is strongest in fall and weakest in spring. For the TC, the northward transport is strongest in winter and weakest in spring, while the southward transport is strongest in fall and weakest in spring, which is associated with the strong southward fiow of the DTC in fall. As the STC, the northward transport is strongest in winter and weakest in summer, while the southward transport is strongest in summer and weakest in spring. This seasonal difference may be associated with the DTC. The zonal wind stress and the east-west slope of sea level play important roles in the seasonal variations of the TC, the STC and the DTC.

Three-dimensional numerical modeling of nearshore circulation

A three-dimensional nearshore circulation model was developed by coupling CH3D, a three-dimensional hydrodynamic model and REF/DIF, a nearshore wave transformation model. The model solves the three-dimensional wave-averaged equations of motion. Wave-induced effects on circulation were introduced in the form of radiation stresses, wave-induced mass transport, wave-induced enhancement of bottom friction and wave-induced turbulent mixing. Effects of breaking waves were considered following Svendsen (1984a and 1984b) and Stive and Wind (1986). The model was successfully tested against the analytical solution of longshore currents by Longuet and Higgins (1970). The model successfully simulated the undertow as observed in a laboratory experiment by Stive and Wind (1982). In addition, the model was applied to a physical model by Mory and Hamm (1997) and successfully reproduced the eddy behind a detached breakwater as well as the longshore current on the open beach and the contiguous eddy in the open area of the wave tank. While the qualitative agreement between model results and experimental observations was very good, the quantitative agreement needs to be further improved. Albeit difficult to explain every discrepancy between the model results and observations, in general, sources of errors are attributed to the lack of understanding and comprehensive description of following processes: (1)the horizontal and vertical distribution of radiation stress, especially for breaking waves;(2)the detailed structure of turbulence;(3)Wave-current interaction (not included at this moment); and (4)the wave-current boundary layer and the resulting bottom shear stress.

Three-Dimensional Structure of Optimal Nonlinear Excitation for Decadal Variability of the Thermohaline Circulation

The decadal variability of the North Atlantic thermohaline circulation(THC) is investigated within a three-dimensional ocean circulation model using the conditional nonlinear optimal perturbation method. The results show that the optimal initial perturbations of temperature and salinity exciting the strongest decadal THC variations have similar structures: the perturbations are mainly in the northwestern basin at a depth ranging from 1500 to 3000 m. These temperature and salinity perturbations act as the optimal precursors for future modifications of the THC, highlighting the importance of observations in the northwestern basin to monitor the variations of temperature and salinity at depth. The decadal THC variation in the nonlinear model initialized by the optimal salinity perturbations is much stronger than that caused by the optimal temperature perturbations, indicating that salinity variations might play a relatively important role in exciting the decadal THC variability. Moreover, the decadal THC variations in the tangent linear and nonlinear models show remarkably different characteristics, suggesting the importance of nonlinear processes in the decadal variability of the THC.

THREE-DIMENSIONAL BAROCLINIC NUMERICAL SIMULATION OF THE SOUTH CHINA SEA CIRCULATION'S SEASONAL CHARACTERISTICS II. MIDDLE AND DEEP CIRCULATION

A three-dimensional baroclinic shelf sea model’s numerical simulation of the South China Sea (SCS) middle and deep layer circulation structure showed that: 1. In the SCS middle and deep layer, a southward boundary current exists along the east shore of the Indo-China Peninsula all year long. A cyclonic eddy (gyre) is formed by the current in the above sea areas except in the middle layer in spring, when an anticyclonic eddy exists on the eastern side of the current. In the deep layer, a large-scale anticyclonic eddy often exists in the sea areas between the Zhongsha Islands and west shore of southern Luzon Island. 2. In the middle layer in summer and autumn, and in the deep layer in autumn and winter, there is an anticyclonic eddy (gyre) in the northeastern SCS, while in the middle layer in winter and spring, and in the deep layer in spring and summer, there is a cyclonic one. 3. In the middle layer, there is a weak northeastward current in the Nansha Trough in spring and summer, while in autumn and winter it evolves into an anticyclonic eddy (gyre), which then spreads westward to the whole western Nansha Islands sea areas.

A three-dimensional ocean general circulation model for mesoscale eddies——Ⅱ Diagnostic analysis

A three-dimensional density field associated with mesoscaie unstable waves generated by the 3-D, primitive-equation model (Wang and Ikeda, 1996) is provided to the quasi-geostrophic pressure tendency and ω-equations, and to the (ageostrophic) Q-vector equation. Diagnostic analyses, analogous to the approaches in meteorology: ω-equation and Q-vector method, are for the first time developed to examine the mesoscaie dynamical processes and mechanisms of the unstable waves propagating in the mid-latitude ocean. The weaknesses and strengths of these two diagnostic approaches are evaluated and compared to the model results. The Q-vector method is then recommended to diagnose the vertical motion associated with the mesoscaie dynamics from a hydrographic CTD (conductivity-temperature-depth) array, while the quasi-geostrophic equations produce some small-scale features (errors) in the diagnosed fields.

An ADI technique for solving three-dimensional coastal circulation

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Three-dimensional numerical simulation of the flow past a circular cylinder based on LES method

The hydrodynamic characteristics of a rigid, single, circular cylinder in a three dimensional, incompressible, uniform cross flow were calculated using the large-eddy simulation method of CFX5. Solutions to the three dimensional N-S equations were obtained by the finite volume method. The focus of this numerical simulation was to research the characteristics of pressure distribution （drag and litt forces） and vortex tubes at high Reynolds numbers. The results of the calculations showed that the forces at every section in the spanwise direction of the cylinder were symmetrical about the middle section and smaller than the forces calculated in two dimensional cases. Moreover, the flow around the cylinder obviously presents three dimensional characteristics.

A theory for three-dimensional response of micropolar plates

Through combined applications of the transfer-matrix method and asymptotic expansion technique,we formulate a theory to predict the three-dimensional response of micropolar plates.No ad hoc assumptions regarding through-thickness assumptions of the field variables are made,and the governing equations are two-dimensional,with the displacements and microrotations of the mid-plane as the unknowns.Once the deformation of the mid-plane is solved,a three-dimensional micropolar elastic field within the plate is generated,which is exact up to the second order except in the boundary region close to the plate edge.As an illustrative example,the bending of a clamped infinitely long plate caused by a uniformly distributed transverse force is analyzed and discussed in detail.

Three-dimensional visualization technology for guiding one-step percutaneous transhepatic cholangioscopic lithotripsy for the treatment of complex hepatolithiasis

BACKGROUND Biliary stone disease is a highly prevalent condition and a leading cause of hospitalization worldwide.Hepatolithiasis with associated strictures has high residual and recurrence rates after traditional multisession percutaneous transhepatic cholangioscopic lithotripsy(PTCSL).AIM To study one-step PTCSL using the percutaneous transhepatic one-step biliary fistulation(PTOBF)technique guided by three-dimensional(3D)visualization.METHODS This was a retrospective,single-center study analyzing,140 patients who,between October 2016 and October 2023,underwent one-step PTCSL for hepatolithiasis.The patients were divided into two groups:The 3D-PTOBF group and the PTOBF group.Stone clearance on choledochoscopy,complications,and long-term clearance and recurrence rates were assessed.RESULTS Age,total bilirubin,direct bilirubin,Child-Pugh class,and stone location were similar between the 2 groups,but there was a significant difference in bile duct strictures,with biliary strictures more common in the 3D-PTOBF group(P=0.001).The median follow-up time was 55.0(55.0,512.0)days.The immediate stone clearance ratio(88.6%vs 27.1%,P=0.000)and stricture resolution ratio(97.1%vs 78.6%,P=0.001)in the 3D-PTOBF group were significantly greater than those in the PTOBF group.Postoperative complication(8.6%vs 41.4%,P=0.000)and stone recurrence rates(7.1%vs 38.6%,P=0.000)were significantly lower in the 3D-PTOBF group.CONCLUSION Three-dimensional visualization helps make one-step PTCSL a safe,effective,and promising treatment for patients with complicated primary hepatolithiasis.The perioperative and long-term outcomes are satisfactory for patients with complicated primary hepatolithiasis.This minimally invasive method has the potential to be used as a substitute for hepatobiliary surgery.

Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor progression.However,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells.This study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I collagen.It also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and migration.Finally,it compares two-dimensional(2D)and 3D cultures.For comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D model.Besides,the two models yielded significantly different cell morphologies.Finally,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D model.These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture systems.The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

Three-dimensional cell-based strategies for liver regeneration

Liver regeneration and the development of effective therapies for liver failure remain formidable challenges in modern medicine.In recent years,the utilization of 3D cell-based strategies has emerged as a promising approach for addressing these urgent clinical requirements.This review provides a thorough analysis of the application of 3D cell-based approaches to liver regeneration and their potential impact on patients with end-stage liver failure.Here,we discuss various 3D culture models that incorporate hepatocytes and stem cells to restore liver function and ameliorate the consequences of liver failure.Furthermore,we explored the challenges in transitioning these innovative strategies from preclinical studies to clinical applications.The collective insights presented herein highlight the significance of 3D cell-based strategies as a transformative paradigm for liver regeneration and improved patient care.

Three-dimensional stability calculation method for high and large composite slopes formed by mining stope and inner dump in adjacent open pits

The 2D limit equilibrium method is widely used for slope stability analysis.However,with the advancement of dump engineering,composite slopes often exhibit significant 3D mechanical effects.Consequently,it is of significant importance to develop an effective 3D stability calculation method for composite slopes to enhance the design and stability control of open-pit slope engineering.Using the composite slope formed by the mining stope and inner dump in Baiyinhua No.1 and No.2 open-pit coal mine as a case study,this research investigates the failure mode of composite slopes and establishes spatial shape equations for the sliding mass.By integrating the shear resistance and sliding force of each row of microstrip columns onto the bottom surface of the strip corresponding to the main sliding surface,a novel 2D equivalent physical and mechanical parameters analysis method for the strips on the main sliding surface of 3D sliding masses is proposed.Subsequently,a comprehensive 3D stability calculation method for composite slopes is developed,and the quantitative relationship between the coordinated development distance and its 3D stability coefficients is examined.The analysis reveals that the failure mode of the composite slope is characterized by cutting-bedding sliding,with the arc serving as the side interface and the weak layer as the bottom interface,while the destabilization mechanism primarily involves shear failure.The spatial form equation of the sliding mass comprises an ellipsoid and weak plane equation.The analysis revealed that when the coordinated development distance is 1500 m,the error rate between the 3D stability calculation result and the 2D stability calculation result of the composite slope is less than 8%,thereby verifying the proposed analytical method of equivalent physical and mechanical parameters and the 3D stability calculation method for composite slopes.Furthermore,the3D stability coefficient of the composite slope exhibits an exponential correlation with the coordinated development distance,with the coefficient gradually decreasing as the coordinated development distance increases.These findings provide a theoretical guideline for designing similar slope shape parameters and conducting stability analysis.

The combined application of stem cells and three-dimensional bioprinting scaffolds for the repair of spinal cord injury

Spinal cord injury is considered one of the most difficult injuries to repair and has one of the worst prognoses for injuries to the nervous system.Following surgery,the poor regenerative capacity of nerve cells and the generation of new scars can make it very difficult for the impaired nervous system to restore its neural functionality.Traditional treatments can only alleviate secondary injuries but cannot fundamentally repair the spinal cord.Consequently,there is a critical need to develop new treatments to promote functional repair after spinal cord injury.Over recent years,there have been seve ral developments in the use of stem cell therapy for the treatment of spinal cord injury.Alongside significant developments in the field of tissue engineering,three-dimensional bioprinting technology has become a hot research topic due to its ability to accurately print complex structures.This led to the loading of three-dimensional bioprinting scaffolds which provided precise cell localization.These three-dimensional bioprinting scaffolds co uld repair damaged neural circuits and had the potential to repair the damaged spinal cord.In this review,we discuss the mechanisms underlying simple stem cell therapy,the application of different types of stem cells for the treatment of spinal cord injury,and the different manufa cturing methods for three-dimensional bioprinting scaffolds.In particular,we focus on the development of three-dimensional bioprinting scaffolds for the treatment of spinal cord injury.

Application of three-dimensional speckle tracking technique in measuring left ventricular myocardial function in patients with diabetes

BACKGROUND Diabetic cardiomyopathy is considered as a chronic complication of diabetes mellitus(DM).Therefore,early detection of left ventricular systolic function(LVSF)damage in DM is essential.AIM To explore the use of the three-dimensional speckle tracking technique(3D-STI)for measuring LVSF in DM patients via meta-analysis.METHODS The electronic databases were retrieved from the initial accessible time to 29 April 2023.The current study involved 9 studies,including 970 subjects.We carried out this meta-analysis to estimate myocardial function in DM compared with controls according to myocardial strain attained by 3D-STI.RESULTS Night articles including 970 subjects were included.No significant difference was detected in the left ventricular ejection fraction between the control and the diabetic group(P>0.05),while differences in global longitudinal strain,global circumferential strain,global radial strain,and global area strain were markedly different between the controls and DM patients(all P<0.05).CONCLUSION The 3D-STI could be applied to accurately measure early LVSF damage in patients with DM.

High-speed autopolarization synchronization modulation three-dimensional structured illumination microscopy

In recent years,notable progress has been achieved in both the hardware and algorithms of structured illumination microscopy(SIM).Nevertheless,the advancement of three-dimensional structured illumination microscopy(3DSIM)has been impeded by challenges arising from the speed and intricacy of polarization modulation.We introduce a high-speed modulation 3DSIM system,leveraging the polarizationmaintaining and modulation capabilities of a digital micromirror device(DMD)in conjunction with an electrooptic modulator.The DMD-3DSIM system yields a twofold enhancement in both lateral(133 nm)and axial(300 nm)resolution compared to wide-field imaging and can acquire a data set comprising 29 sections of 1024 pixels×1024 pixels,with 15 ms exposure time and 6.75 s per volume.The versatility of the DMD-3DSIM approach was exemplified through the imaging of various specimens,including fluorescent beads,nuclear pores,microtubules,actin filaments,and mitochondria within cells,as well as plant and animal tissues.Notably,polarized 3DSIM elucidated the orientation of actin filaments.Furthermore,the implementation of diverse deconvolution algorithms further enhances 3D resolution.The DMD-based 3DSIM system presents a rapid and reliable methodology for investigating biomedical phenomena,boasting capabilities encompassing 3D superresolution,fast temporal resolution,and polarization imaging.

Virtual and augmented reality systems and three-dimensional printing of the renal model—novel trends to guide preoperative planning for renal cancer

Objective:This study aimed to explore the applications of three-dimensional (3D) technology, including virtual reality, augmented reality (AR), and 3D printing system, in the field of medicine, particularly in renal interventions for cancer treatment.Methods:A specialized software transforms 2D medical images into precise 3D digital models, facilitating improved anatomical understanding and surgical planning. Patient-specific 3D printed anatomical models are utilized for preoperative planning, intraoperative guidance, and surgical education. AR technology enables the overlay of digital perceptions onto real-world surgical environments.Results:Patient-specific 3D printed anatomical models have multiple applications, such as preoperative planning, intraoperative guidance, trainee education, and patient counseling. Virtual reality involves substituting the real world with a computer-generated 3D environment, while AR overlays digitally created perceptions onto the existing reality. The advances in 3D modeling technology have sparked considerable interest in their application to partial nephrectomy in the realm of renal cancer. 3D printing, also known as additive manufacturing, constructs 3D objects based on computer-aided design or digital 3D models. Utilizing 3D-printed preoperative renal models provides benefits for surgical planning, offering a more reliable assessment of the tumor's relationship with vital anatomical structures and enabling better preparation for procedures. AR technology allows surgeons to visualize patient-specific renal anatomical structures and their spatial relationships with surrounding organs by projecting CT/MRI images onto a live laparoscopic video. Incorporating patient-specific 3D digital models into healthcare enhances best practice, resulting in improved patient care, increased patient satisfaction, and cost saving for the healthcare system.

Numerical simulations and comparative analysis of two- and three-dimensional circulating fluidized bed reactors for CO2 capture

Carbon dioxide(CO2),the main gas emitted from fossil burning,is the primary contributor to global warming.Circulating fluidized bed reactor(CFBR)is proved as an energy-efficient method for post-combustion CO2 capture.The numerical simulation by computational fluid dynamics(CFD)is believed as a promising tool to study CO2 adsorption process in CFBR.Although three-dimensional(3D)simulations were proved to have better predicting performance with the experimental results,two-dimensional(2D)simulations have been widely reported for qualitative and quantitative studies on gas-solid behavior in CFBR for its higher computational efficiency recently.However,the discrepancies between 2D and 3D simulations have rarely been evaluated by detailed study.Considering that the differences between the 2D and 3D simulations will vary substantially with the changes of independent operating conditions,it is beneficial to lower computational costs to clarify the effects of dimensionality on the numerical CO2 adsorption runs under various operating conditions.In this work,the comparative analysis for CO2 adsorption in 2D and 3D simulations was conducted to enlighten the effects of dimensionality on the hydrodynamics and reaction behaviors,in which the separation rate,species distribution and hydrodynamic characteristics were comparatively studied for both model frames.With both accuracy and computational costs considered,the viable suggestions were provided in selecting appropriate model frame for the studies on optimization of operating conditions,which directly affect the capture and energy efficiencies of cyclic CO2 capture process in CFBR.