To regenerate three-dimensional endometrium in vitro as a novel model for studying the mechanism of implantation of embryos, the luminal epithelial cells and stromal cells of the rabbit uterus were separated and cultu...To regenerate three-dimensional endometrium in vitro as a novel model for studying the mechanism of implantation of embryos, the luminal epithelial cells and stromal cells of the rabbit uterus were separated and cultured in vitro. The type Ⅰ mouse tail collagen was used as scaffolding material. The stromal cells were inoculated in the type I mouse tail collagen, and the luminal epithelial cells were inoculated on the type i mouse tall collagen to regenerate the endometrium in vitro. The regenerated endometrium was cultured in DMEM-F/12 media containing 100 nmol L^-1 progesterone, 10 nM β-estradiol, and 10% fetal bovine serum （FBS） for 3 d. The media were then replaced with CZB containing 100 nM progesterone, 10 nmol L-1 β-estradiol, and 10% FBS, and the mouse blastulas were co-cultured with it. The results of scanning electronic micrography showed that the epithelial cells on the surface of the reconstructed endometrium were covered with numerous slender microvilli and some epithelial cells protruded pinopodes. After culturing for 12 h with the mouse blastula, the shedding, attachment, and implantation of the blastula were observed. The blastula can escape from zona pellucida and attach to the three-dimensional endometrium and is then implanted into it. This study showed that the reconstructed three-dimensional endometrium can serve as a robust embryo implantation model in vitro.展开更多
Successful embryo implantation requires highly coordinated maternal-embryo interactions.Implantation failure is a major factor contributing to infertility.However,the mechanism underlying implantation failure remains ...Successful embryo implantation requires highly coordinated maternal-embryo interactions.Implantation failure is a major factor contributing to infertility.However,the mechanism underlying implantation failure remains unclear.An improved understanding of the early implantation process not only improves the success rate of assisted reproductive treatments but also helps in studying the pathophysiology of reproductive disorders.Owing to ethical concerns,in vivo studies of human embryo implantation are not feasible.However,the results obtained from animal models cannot be directly applied to humans.Over the years,in vitro implantation models have been developed to investigate implantation mechanisms.In this review,we discuss the use of different models for generating embryo-like surrogates to study early embryo development and implantation in vitro,with a specific focus on stem cell-derived blastocyst-like embryo surrogates.There is no definitive evidence that the recently established embryo-like models re-capitulate all developmental events of human embryos during the peri-implantation stage.Regardless,stem cell-derived embryo surrogates are the most valuable tools for studying the mechanisms of early cell lineage differentiation and developmental failures during implantation.展开更多
文摘To regenerate three-dimensional endometrium in vitro as a novel model for studying the mechanism of implantation of embryos, the luminal epithelial cells and stromal cells of the rabbit uterus were separated and cultured in vitro. The type Ⅰ mouse tail collagen was used as scaffolding material. The stromal cells were inoculated in the type I mouse tail collagen, and the luminal epithelial cells were inoculated on the type i mouse tall collagen to regenerate the endometrium in vitro. The regenerated endometrium was cultured in DMEM-F/12 media containing 100 nmol L^-1 progesterone, 10 nM β-estradiol, and 10% fetal bovine serum （FBS） for 3 d. The media were then replaced with CZB containing 100 nM progesterone, 10 nmol L-1 β-estradiol, and 10% FBS, and the mouse blastulas were co-cultured with it. The results of scanning electronic micrography showed that the epithelial cells on the surface of the reconstructed endometrium were covered with numerous slender microvilli and some epithelial cells protruded pinopodes. After culturing for 12 h with the mouse blastula, the shedding, attachment, and implantation of the blastula were observed. The blastula can escape from zona pellucida and attach to the three-dimensional endometrium and is then implanted into it. This study showed that the reconstructed three-dimensional endometrium can serve as a robust embryo implantation model in vitro.
基金supported in part by a General Research Fund(grant number:17111414)Research Grants Council of Hong Kong+3 种基金Health and Medical Research Fund(grant numbers:HMRF 04151546)Food and Health Bureau,Government of the Hong Kong Special Administrative RegionShenzhen Science and Technology Program(KQTD20190929172749226)The University of Hong Kong-Shenzhen Hospital Fund for Shenzhen Key Medical Discipline(SZXK2020089)
文摘Successful embryo implantation requires highly coordinated maternal-embryo interactions.Implantation failure is a major factor contributing to infertility.However,the mechanism underlying implantation failure remains unclear.An improved understanding of the early implantation process not only improves the success rate of assisted reproductive treatments but also helps in studying the pathophysiology of reproductive disorders.Owing to ethical concerns,in vivo studies of human embryo implantation are not feasible.However,the results obtained from animal models cannot be directly applied to humans.Over the years,in vitro implantation models have been developed to investigate implantation mechanisms.In this review,we discuss the use of different models for generating embryo-like surrogates to study early embryo development and implantation in vitro,with a specific focus on stem cell-derived blastocyst-like embryo surrogates.There is no definitive evidence that the recently established embryo-like models re-capitulate all developmental events of human embryos during the peri-implantation stage.Regardless,stem cell-derived embryo surrogates are the most valuable tools for studying the mechanisms of early cell lineage differentiation and developmental failures during implantation.
