Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor progression.However,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells.This study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I collagen.It also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and migration.Finally,it compares two-dimensional(2D)and 3D cultures.For comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D model.Besides,the two models yielded significantly different cell morphologies.Finally,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D model.These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture systems.The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

Virtual and augmented reality systems and three-dimensional printing of the renal model—novel trends to guide preoperative planning for renal cancer

ObjectiveThis study aimed to explore the applications of three-dimensional (3D) technology, including virtual reality, augmented reality (AR), and 3D printing system, in the field of medicine, particularly in renal interventions for cancer treatment.MethodsA specialized software transforms 2D medical images into precise 3D digital models, facilitating improved anatomical understanding and surgical planning. Patient-specific 3D printed anatomical models are utilized for preoperative planning, intraoperative guidance, and surgical education. AR technology enables the overlay of digital perceptions onto real-world surgical environments.ResultsPatient-specific 3D printed anatomical models have multiple applications, such as preoperative planning, intraoperative guidance, trainee education, and patient counseling. Virtual reality involves substituting the real world with a computer-generated 3D environment, while AR overlays digitally created perceptions onto the existing reality. The advances in 3D modeling technology have sparked considerable interest in their application to partial nephrectomy in the realm of renal cancer. 3D printing, also known as additive manufacturing, constructs 3D objects based on computer-aided design or digital 3D models. Utilizing 3D-printed preoperative renal models provides benefits for surgical planning, offering a more reliable assessment of the tumor's relationship with vital anatomical structures and enabling better preparation for procedures. AR technology allows surgeons to visualize patient-specific renal anatomical structures and their spatial relationships with surrounding organs by projecting CT/MRI images onto a live laparoscopic video. Incorporating patient-specific 3D digital models into healthcare enhances best practice, resulting in improved patient care, increased patient satisfaction, and cost saving for the healthcare system.

Three-dimensional structural models,evolution and petroleum geological significances of transtensional faults in the Ziyang area,central Sichuan Basin,SW China

With drilling and seismic data of Transtensional(strike-slip)Fault System in the Ziyang area of the central Sichuan Basin,SW China plane-section integrated structural interpretation,3-D fault framework model building,fault throw analyzing,and balanced profile restoration,it is pointed out that the transtensional fault system in the Ziyang 3-D seismic survey consists of the northeast-trending F_(I)19 and F_(I)20 fault zones dominated by extensional deformation,as well as 3 sets of northwest-trending en echelon normal faults experienced dextral shear deformation.Among them,the F_(I)19 and F_(I)20 fault zones cut through the Neoproterozoic to Lower Triassic Jialingjiang Formation,presenting a 3-D structure of an“S”-shaped ribbon.And before Permian and during the Early Triassic,the F_(I)19 and F_(I)20 fault zones underwent at least two periods of structural superimposition.Besides,the 3 sets of northwest-trending en echelon normal faults are composed of small normal faults arranged in pairs,with opposite dip directions and partially left-stepped arrangement.And before Permian,they had formed almost,restricting the eastward growth and propagation of the F_(I)19 fault zone.The F_(I)19 and F_(I)20 fault zones communicate multiple sets of source rocks and reservoirs from deep to shallow,and the timing of fault activity matches well with oil and gas generation peaks.If there were favorable Cambrian-Triassic sedimentary facies and reservoirs developing on the local anticlinal belts of both sides of the F_(I)19 and F_(I)20 fault zones,the major reservoirs in this area are expected to achieve breakthroughs in oil and gas exploration.

Hyper accuracy three-dimensional virtual anatomical rainbow model facilitates surgical planning and safe selective clamping during robot-assisted partial nephrectomy

ObjectiveTo highlight the role of hyper accuracy three-dimensional(3D)reconstruction in facilitating surgical planning and guiding selective clamping during robot-assisted partial nephrectomy(RAPN).MethodsA transperitoneal RAPN was performed in a 62-year-old male patient presenting with a 4 cm right anterior interpolar renal mass(R.E.N.A.L nephrometry score 7A).An abnormal vasculature was observed,with a single renal vein and two right renal arteries originating superiorly to the vein and anterior,when dividing in their segmental branches.According to the hyper accuracy 3D(HA3D^(®))rainbow model(MEDICS Srl,Turin,Italy),one branch belonging to one of the segmental arteries was feeding the tumor.This allowed for an accurate prediction of the area vascularized by each arterial branch.The 3D model was included in the intraoperative console view during the whole procedure,using the TilePro feature.A step-by-step explanation of the procedure is provided in the video attached to the present article.ResultsThe operative time was 90 min with a warm ischemia time on selective clamping of 13 min.Estimated blood loss was 180 mL.No intraoperative complication was encountered and no drain was placed at the end of the procedure.The patient was discharged on postoperative Day 2,without any early postoperative complications.The final pathology report showed a pathological tumor stage 1 clear cell renal cell carcinoma with negative surgical margins.ConclusionThe present study and the attached video illustrate the value of 3D rainbow model during the planning and execution of a RAPN with selective clamping.It shows how the surgeon can rely on this model to be more efficient by avoiding unnecessary surgical steps,and to safely adopt a“selective”clamping strategy that can translate in minimal functional impact.

Three-dimensional Computational Fluid Dynamics Modeling of Two-phase Flow in a Structured Packing Column

Characterizing the complex two-phase hydrodynamics in structured packed columns requires a power- ful modeling tool. The traditional two-dimensional model exhibits limitations when one attempts to model the de- tailed two-phase flow inside the columns. The present paper presents a three-dimensional computational fluid dy- namics （CFD） model to simulate the two-phase flow in a representative unit of the column. The unit consists of an CFD calculations on column packed with Flexipak 1Y were implemented within the volume of fluid （VOF） mathe- matical framework. The CFD model was validated by comparing the calculated thickness of liquid film with the available experimental data. Special attention was given to quantitative analysis of the effects of gravity on the hy- drodynamics. Fluctuations in the liquid mass flow rate and the calculated pressure drop loss were found to be quali- tatively in agreement with the experimental observations.

THE STUDY ON THREE-DIMENSIONAL MATHEMATICAL MODEL OF RIVER BED EROSION FOR WATER-SEDIMENT TWO-PHASE FLOW

Based on the tensor analysis of water-sediment two-phase how, the basic model equations for clear water flow and sediment-laden flow are deduced in the general curve coordinates for natural water variable-density turbulent how. Furthermore, corresponding boundary conditions are also presented in connection with the composition and movement of non-uniform bed material. The theoretical results are applied to the calculation of the float open caisson in the construction period and good results are obtained.

Three-dimensional turbulent model of heat transfer and fluid flow in GTAW process

A two-equation K-ε turbulent fluid flow model is built to model the heat transfer and fluid flow in gas tungsten arc welding （GTAW） process of stainless steel S US310 and S US316. This model combines the buoyancy force, lorentz force and marangni force as the driving forces of thefluidflow in the weld pool. The material properties are functions of temperature in this model. The simulated results show that the molten metal flowing outward is mainly caused by the marangoni convection, which makes the weld pool become wider and shallower. The comparison of the weld pool shape of SUS310 and SUS316 shows that the slight differences of the value of thermal conductivity mainly attributes to the difference of the weld pool shape and the distinction of heat transport in laminar and turbulent model makes large diversity in the simulated results.

A Higher-Efficient Non-Hydrostatic Finite Volume Model for Strong Three-Dimensional Free Surface Flows and Sediment Transport

In order to accurately simulate strong three-dimensional （3-D） free surface flows and sediment transport, the fully 3- D non-hydrostatic pressure models are developed based on the incompressible Navier-Stokes equations and convection-diffusion equation of sediment concentration with the mixing triangle and quadrilateral grids. The governing equations are discretized with the unstructured finite volume method in order to provide conservation properties of mass and momentum, and flexibility with practical application. It is shown that it is first-order accurate on nonuniform plane two-dimensional （2-D） grids and second-order accurate on uniform plane grids. A third-order approximation of the vertical velocity at the top-layer is applied. In such a way, free surface zero stress boundary condition is satisfied maturely, and very few vertical layers are needed to give an accurate solution even for complex discontinuous flow and short wave simulation. The model is applied to four examples to simulate strong 3-D free surface flows and sediment transport where non-hydrostatic pressures have a considerable effect on the velocity field. The newly developed model is verified against analytical solutions with an excellent agreement.

The Design of a Three-Dimensional Physical Modeling System for Real-Time Groundwater Flows

In the past decades,physical modeling has been widely used in hydrogeology for teaching,studying and exhibition purposes.Most of these models are used to illustrate hydrogeological profiles,but few can depict three-dimensional groundwater flows,making it impossible to validate groundwater flows simulated by numerical methods with physical modeling.

Three-dimensional boundary layer flow of Maxwell nanofluid:mathematical model

The present research explores the three-dimensional boundary layer flow of the Maxwell nanofluid. The flow is generated by a bidirectional stretching surface. The mathematical formulation is carried out through a boundary layer approach with the heat source/sink, the Brownian motion, and the thermophoresis effects. The newly developed boundary conditions requiring zero nanoparticle mass flux at the boundary are employed in the flow analysis for the Maxwell fluid. The governing nonlinear boundary layer equations through appropriate transformations are reduced to the coupled nonlin- ear ordinary differential system. The resulting nonlinear system is solved. Graphs are plotted to examine the effects of various interesting parameters on the non-dimensional velocities, temperature, and concentration fields. The values of the local Nusselt number are computed and examined numerically.

Three-dimensional CFD simulation of inlet structure flow in pumping station based on Eulerian solid- liquid two-phase flow model

Sediment deposition in the pumping station has a huge negative impact on unit operation.The three-dimensional CFD method has been used to simulate inlet structure flow in pumping station based on the Eulerian solid- liquid two-phase flow model. The numerical results of the preliminary scheme show that sediment deposition occurs in the forebay of pumping station because of poor flow pattern therein. In order to improve hydraulic configuration in the forebay,one modified measure reconstructs water diversion weir shape,and another measure sets a water retaining sill in the approach channel. The simulation results of the modified scheme prove that back flow in the forebay has been eliminated and the sediment deposition region has also been reduced.

A Higher-Efficient Three-Dimensional Numerical Model for Small Amplitude Free Surface Flows

A higher-efficient three-dimensional non-hydrostatic model is developed to simulate small amplitude free surface flows based on a staggered unstructured grid. In this model, a fractional step algorithm is adopted to solve the Navier-Stokes equations in two major steps. A top-layer pressure method is proposed to minimize the number of vertical layers and subsequently the computational cost. Three classical examples of small amplitude free surface flows are used to demonstrate the capability and efficiency of the model. The satisfactory results demonstrated the capability and efficiency of modelling a range of small amplitude free surface flows with only a small number of vertical layers.

NUMERICAL INVESTIGATION OF THREE-DIMENSIONAL VISCOUS INCOMPRESSIBLE FLOWS IN DIVERGENT CURVED CHANNELS AND TURBULENT MODEL STUDY

In order to make the numerical calculation of viscous flows more convenient for the flows in channel with complicated profile governing equations expressed in the arbitrary curvilinear coordinates were derived by means of Favre density-weighted averaged method, and a turbulent model with effect of curvature modification was also derived. The numerical calculation of laminar and turbulent flown in divergent curved channels was carried out by means of parabolizeil computation method. The calculating results were used to analyze and investigate the aerodynamic performance of talor cascades in compressors preliminarily.

Explicit Algebraic Stress Model for Three-Dimensional Turbulent Buoyant Flows Derived Using Tensor Representation

An explicit algebraic stress model (EASM) has been formulated for two-dimensional turbulent buoyant flows using a five-term tensor representation in a prior study. The derivation was based on partitioning the buoyant flux tensor into a two-dimensional and a three-dimensional component. The five-term basis was formed with the two-dimensional component of the buoyant flux tensor. As such, the derived EASM is limited to two-dimensional flows only. In this paper, a more general approach using a seven-term representation without partitioning the buoyant flux tensor is used to derive an EASM valid for two- and three-dimensional turbulent buoyant flows. Consequently, the basis tensors are formed with the fully three-dimensional buoyant flux tensor. The derived EASM has the two-dimensional flow as a special case. The matrices and the representation coefficients are further simplified using a four-term representation. When this four-term representation model is applied to calculate two-dimensional homogeneous buoyant flows, the results are essentially identical with those obtained previously using the two-dimensional component of the buoyant flux tensor. Therefore, the present approach leads to a more general EASM formulation that is equally valid for two- and three-dimensional turbulent buoyant flows.

Three-Dimensional Modelling of a DC Arc in Cross-Flow

A commercial CFD （computational fluid dynamics） code FLUENT was used and modified to model an atmospheric pressure argon arc in a low cross flow by solving the fully coupled conservation equations. Numerical experiments, with an arc current of 100 A to 200 A, an arcing distance of 3 mm to 6 mm, and a cross-flow velocity of 10 m/s to 30 m/s, were carried out. The modelling results show that the arc tends to take the shortest path to the anode when deflected by the cross flow; its anode attachment is farther downstream than the cathode one. Furthermore, due to the low input gas flow imposed in this study, the effect of electromagnetic force is important and it influences the crosscut shape of the arc significantly.

Three-dimensional simulation of pore scale fluid flow in granular ore media with realistic geometry

The images of granular ore media were captured by X-ray CT scanner. Combined with digital image processing and finite element techniques, the three-dimensional geometrical model, which represents the realistic pore structure of the media, was constructed. With this model, three dimensional pore scale fluid flow among particles was simulated. Then the distributions of fluid flow velocity and pressure were analyzed and the hydraulic conductivity was calculated. The simulation results indicate the fluid flow behaviors are mainly dominated by the volume and topological structure of pore space. There exist obvious preferential flow and leaching blind zones simultaneously in the medium. The highest velocities generally occur in those narrow pores with high pressure drops. The hydraulic conductivity obtained by simulation is the same order of magnitude as the laboratory test result, which denotes the validity of the model. The pore-scale and macro-scale are combined and the established geometrical model can be used for the simulations of other phenomena during heap leaching process.

Three-dimensional numerical simulation of the flow past a circular cylinder based on LES method

The hydrodynamic characteristics of a rigid, single, circular cylinder in a three dimensional, incompressible, uniform cross flow were calculated using the large-eddy simulation method of CFX5. Solutions to the three dimensional N-S equations were obtained by the finite volume method. The focus of this numerical simulation was to research the characteristics of pressure distribution （drag and litt forces） and vortex tubes at high Reynolds numbers. The results of the calculations showed that the forces at every section in the spanwise direction of the cylinder were symmetrical about the middle section and smaller than the forces calculated in two dimensional cases. Moreover, the flow around the cylinder obviously presents three dimensional characteristics.

Thickness-averaged model for numerical simulation of electroosmotic flow in three-dimensional microfluidic chips

The microfiuidic system is a multi-physics interaction field that has at- tracted great attention. The electric double layers and electroosmosis are important flow-electricity interaction phenomena. This paper presents a thickness-averaged model to solve three-dimensional complex electroosmotic flows in a wide-shallow microchan- nel/chamber combined （MCC） chip based on the Navier-Stokes equations for the flow field and the Poisson equation to the electric field. Behaviors of the electroosmotic flow, the electric field, and the pressure are analyzed. The quantitative effects of the wall charge density （or the zeta potential） and the applied electric field on the electroosmotic flow rate are investigated. The two-dimensional thickness-averaged flow model greatly simplifies the three-dimensional computation of the complex electroosmotic flows, and correctly reflects the electrookinetic effects of the wall charge on the flow. The numerical results indicate that the electroosmotic flow rate of the thickness-averaged model agrees well with that of the three-dimensional slip-boundary flow model. The flow streamlines and pressure distribution of these two models are in qualitative agreement.

Three-Dimensional Tidal Model and Its Application to Numerical Simulation of Water Quality in Coastal Waters

The turbulence mechanism plays an important part in the mixing process and momentum transfer of turbulence. A three-dimensional Prandtl mixing length tidal model has been developed to simulate tidal flows and water quality. The eddy viscosities and diffusivities are computed from the Prandtl mixing length model. In order to model the water quality of an estuary or coastal area many interdependent processes need to be simulated. These may be conveniently separated into three main groups: transport and mixing processes, biochemical interaction of water quality variables and the utilization and re-cycling of nutrients by living matter. The model simulates full oxygen and nutrient balance, primary productivity and the transport, reaction mechanism and fate of pollutants over tidal time-scales. The model is applied to numerical simulation of tidal flows and water quality in Dalian Bay. The model has been calibrated against a limited data set of historical water quality observations and in general demonstrates excellent agreement with all available data.

DEVELOPMENT OF A HYBRID MODEL FOR THREE-DIMENSIONAL GIS

This paper presents a hybrid model for three-dimensional Geographical Information Systems which is an integration of surface- and volume-based models. The Triangulated Irregular Network (TIN) and octree models are integrated in this hybrid models. The TIN model works as a surface-based model which mainly serves for surface presentation and visualization. On the other hand, the octree encoding supports volumetric analysis. The designed data structure brings a major advantage in the three-dimensional selective retrieval. This technique increases the efficiency of three-dimensional data operation.