Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor progression.However,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells.This study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I collagen.It also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and migration.Finally,it compares two-dimensional(2D)and 3D cultures.For comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D model.Besides,the two models yielded significantly different cell morphologies.Finally,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D model.These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture systems.The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

Improving model performance in mapping cropland soil organic matter using time-series remote sensing data

Faced with increasing global soil degradation,spatially explicit data on cropland soil organic matter(SOM)provides crucial data for soil carbon pool accounting,cropland quality assessment and the formulation of effective management policies.As a spatial information prediction technique,digital soil mapping(DSM)has been widely used to spatially map soil information at different scales.However,the accuracy of digital SOM maps for cropland is typically lower than for other land cover types due to the inherent difficulty in precisely quantifying human disturbance.To overcome this limitation,this study systematically assessed a framework of“information extractionfeature selection-model averaging”for improving model performance in mapping cropland SOM using 462 cropland soil samples collected in Guangzhou,China in 2021.The results showed that using the framework of dynamic information extraction,feature selection and model averaging could efficiently improve the accuracy of the final predictions(R^(2):0.48 to 0.53)without having obviously negative impacts on uncertainty.Quantifying the dynamic information of the environment was an efficient way to generate covariates that are linearly and nonlinearly related to SOM,which improved the R^(2)of random forest from 0.44 to 0.48 and the R^(2)of extreme gradient boosting from 0.37to 0.43.Forward recursive feature selection(FRFS)is recommended when there are relatively few environmental covariates(<200),whereas Boruta is recommended when there are many environmental covariates(>500).The Granger-Ramanathan model averaging approach could improve the prediction accuracy and average uncertainty.When the structures of initial prediction models are similar,increasing in the number of averaging models did not have significantly positive effects on the final predictions.Given the advantages of these selected strategies over information extraction,feature selection and model averaging have a great potential for high-accuracy soil mapping at any scales,so this approach can provide more reliable references for soil conservation policy-making.

Kinematic-mapping-model-guided analysis and optimization of 2-PSS&1-RR circular-rail parallel mechanism for fully steerable phased array antennas

This paper presents a systematic methodology for analyzing and optimizing an innovative antenna mount designed for phased array antennas, implemented through a novel 2-PSS&1-RR circular-rail parallel mechanism. Initially, a comparative motion analysis between the 3D model of the mount and its full-scale prototype is conducted to validate effectiveness. Given the inherent complexity, a kinematic mapping model is established between the mount and the crank-slider linkage, providing a guiding framework for subsequent analysis and optimization. Guided by this model, feasible inverse and forward solutions are derived, enabling precise identification of stiffness singularities. The concept of singularity distance is thus introduced to reflect the structural stiffness of the mount. Subsequently, also guided by the mapping model, a heuristic algorithm incorporating two backtracking procedures is developed to reduce the mount's mass. Additionally, a parametric finite-element model is employed to explore the relation between singularity distance and structural stiffness. The results indicate a significant reduction(about 16%) in the antenna mount's mass through the developed algorithm, while highlighting the singularity distance as an effective stiffness indicator for this type of antenna mount.

Three-dimensional structural models,evolution and petroleum geological significances of transtensional faults in the Ziyang area,central Sichuan Basin,SW China

With drilling and seismic data of Transtensional(strike-slip)Fault System in the Ziyang area of the central Sichuan Basin,SW China plane-section integrated structural interpretation,3-D fault framework model building,fault throw analyzing,and balanced profile restoration,it is pointed out that the transtensional fault system in the Ziyang 3-D seismic survey consists of the northeast-trending F_(I)19 and F_(I)20 fault zones dominated by extensional deformation,as well as 3 sets of northwest-trending en echelon normal faults experienced dextral shear deformation.Among them,the F_(I)19 and F_(I)20 fault zones cut through the Neoproterozoic to Lower Triassic Jialingjiang Formation,presenting a 3-D structure of an“S”-shaped ribbon.And before Permian and during the Early Triassic,the F_(I)19 and F_(I)20 fault zones underwent at least two periods of structural superimposition.Besides,the 3 sets of northwest-trending en echelon normal faults are composed of small normal faults arranged in pairs,with opposite dip directions and partially left-stepped arrangement.And before Permian,they had formed almost,restricting the eastward growth and propagation of the F_(I)19 fault zone.The F_(I)19 and F_(I)20 fault zones communicate multiple sets of source rocks and reservoirs from deep to shallow,and the timing of fault activity matches well with oil and gas generation peaks.If there were favorable Cambrian-Triassic sedimentary facies and reservoirs developing on the local anticlinal belts of both sides of the F_(I)19 and F_(I)20 fault zones,the major reservoirs in this area are expected to achieve breakthroughs in oil and gas exploration.

Estimation and mapping of water erosion and soil loss:Application of Gavrilovic erosion potential model(EPM)using GIS and remote sensing in the Assif el mal Watershed,Western high Atlas

Water erosion is a serious problem that leads to soil degradation,loss,and the destruction of structures.Assessing the risk of erosion and determining the affected areas has become crucial in order to understand the main factors influencing its evolution and to minimize its impacts.This study focuses on evaluating the risk of erosion in the Assif el mal watershed,which is located in the High Atlas Mountains.The Erosion Potential Model(EPM)is used to estimate soil losses depending on various parameters such as lithology,hydrology,topography,and morphometry.Geographic information systems and remote sensing techniques are employed to map areas with high erosive potential and their relationship with the distribution of factors involved.Different digital elevation models are also used in this study to highlight the impact of data quality on the accuracy of the results.The findings reveal that approximately 59%of the total area in the Assif el mal basin has low to very low potential for soil losses,while 22%is moderately affected and 19.9%is at high to very high risk.It is therefore crucial to implement soil conservation measures to mitigate and prevent erosion risks.

Imaging simulation and analysis of attitude jitter effect on topographic mapping for lunar orbiter stereo optical cameras

The geometric accuracy of topographic mapping with high-resolution remote sensing images is inevita-bly affected by the orbiter attitude jitter.Therefore,it is necessary to conduct preliminary research on the stereo mapping camera equipped on lunar orbiter before launching.In this work,an imaging simulation method consid-ering the attitude jitter is presented.The impact analysis of different attitude jitter on terrain undulation is conduct-ed by simulating jitter at three attitude angles,respectively.The proposed simulation method is based on the rigor-ous sensor model,using the lunar digital elevation model(DEM)and orthoimage as reference data.The orbit and attitude of the lunar stereo mapping camera are simulated while considering the attitude jitter.Two-dimensional simulated stereo images are generated according to the position and attitude of the orbiter in a given orbit.Experi-mental analyses were conducted by the DEM with the simulated stereo image.The simulation imaging results demonstrate that the proposed method can ensure imaging efficiency without losing the accuracy of topographic mapping.The effect of attitude jitter on the stereo mapping accuracy of the simulated images was analyzed through a DEM comparison.

Effects of three-dimensional quality assessment nursing intervention on efficacy and disease management of patients undergoing esophageal cancer surgery

BACKGROUND Esophageal cancer is one of the most common malignant tumors.The three-dimensional quality structure model is a quality assessment theory that includes three dimensions:Structure,process,and results.AIM To investigate the effects of nursing interventions with three-dimensional quality assessment on the efficacy and disease management ability of patients undergoing esophageal cancer surgery.METHODS In this prospective study,the control group received routine nursing,and the intervention group additionally received a three-dimensional quality assessment intervention based on the above routine care.Self-efficacy and patient disease management abilities were evaluated using the General Self-Efficacy Scale(GSES)and Exercise of Self-Care Agency scale,respectively.IBM SPSS Statistics for Windows,version 17.0,was used for the data processing.RESULTS This study recruited 112 patients who were assigned to the control and experi-mental groups(n=56 per group).Before the intervention,there was no significant difference in GSES scores between the two groups(P>0.05).After the inter-vention,the GSES scores of both groups increased,with the experimental group showing higher values(P<0.05).At the time of discharge and three months after discharge,the scores for positive attitudes,self-stress reduction,and total score of health promotion in the experimental group were higher than those in the control group(P<0.05).CONCLUSION The implementation of a three-dimensional quality structure model for postoperative patients with esophageal cancer can effectively improve their self-management ability and self-efficacy of postoperative patients.

Intrusion Detection Model Using Chaotic MAP for Network Coding Enabled Mobile Small Cells

Wireless Network security management is difficult because of the ever-increasing number of wireless network malfunctions,vulnerabilities,and assaults.Complex security systems,such as Intrusion Detection Systems(IDS),are essential due to the limitations of simpler security measures,such as cryptography and firewalls.Due to their compact nature and low energy reserves,wireless networks present a significant challenge for security procedures.The features of small cells can cause threats to the network.Network Coding(NC)enabled small cells are vulnerable to various types of attacks.Avoiding attacks and performing secure“peer”to“peer”data transmission is a challenging task in small cells.Due to the low power and memory requirements of the proposed model,it is well suited to use with constrained small cells.An attacker cannot change the contents of data and generate a new Hashed Homomorphic Message Authentication Code(HHMAC)hash between transmissions since the HMAC function is generated using the shared secret.In this research,a chaotic sequence mapping based low overhead 1D Improved Logistic Map is used to secure“peer”to“peer”data transmission model using lightweight H-MAC(1D-LM-P2P-LHHMAC)is proposed with accurate intrusion detection.The proposed model is evaluated with the traditional models by considering various evaluation metrics like Vector Set Generation Accuracy Levels,Key Pair Generation Time Levels,Chaotic Map Accuracy Levels,Intrusion Detection Accuracy Levels,and the results represent that the proposed model performance in chaotic map accuracy level is 98%and intrusion detection is 98.2%.The proposed model is compared with the traditional models and the results represent that the proposed model secure data transmission levels are high.

Asymmetric image encryption algorithm based on a new three-dimensional improved logistic chaotic map

Based on some analyses of existing chaotic image encryption frameworks and a new designed three-dimensional improved logistic chaotic map(3D-ILM),an asymmetric image encryption algorithm using public-key Rivest–Shamir–Adleman(RSA)is presented in this paper.In the first stage,a new 3D-ILM is proposed to enhance the chaotic behavior considering analysis of time sequence,Lyapunov exponent,and Shannon entropy.In the second stage,combined with the public key RSA algorithm,a new key acquisition mathematical model(MKA)is constructed to obtain the initial keys for the 3D-ILM.Consequently,the key stream can be produced depending on the plain image for a higher security.Moreover,a novel process model(NPM)for the input of the 3D-ILM is built,which is built to improve the distribution uniformity of the chaotic sequence.In the third stage,to encrypt the plain image,a pre-process by exclusive OR(XOR)operation with a random matrix is applied.Then,the pre-processed image is performed by a permutation for rows,a downward modulo function for adjacent pixels,a permutation for columns,a forward direction XOR addition-modulo diffusion,and a backward direction XOR addition-modulo diffusion to achieve the final cipher image.Moreover,experiments show that the the proposed algorithm has a better performance.Especially,the number of pixels change rate(NPCR)is close to ideal case 99.6094%,with the unified average changing intensity(UACI)close to 33.4634%,and the information entropy(IE)close to 8.

Research on Three-Dimensional Simulation of the Internal Arc Gear Skiving

Aiming at the problems that the simulation accuracy which is reduced due to the simplification of the model,a three-dimensional simulation method based on solid modeling is being proposed.By analyzing the motion relationship and positional relationship between the caries knife and the workpiece,the coordinate system of the caries machining was established.With the MATLAB software,the cutting edge model and the blade sweeping surface model of the boring cutter are sequentially established.Boolean operation is performed on the blade swept surface formed by the tooth cutter teeth with time t and the workpiece tooth geometry as well as the undeformed three-dimensional chip geometry model and the instantaneous cogging geometry model are obtained at different times.Through the compare between gear end face simulation tooth profile and the theoretical inner arc tooth profile,we verified the accuracy and rationality of the proposed method.

Study of the geomagnetic field's regional gradients in Chinese continent using three-dimensional surface Spline model

We combined domestic ground-based and satellite magnetic measurements to create a regional three-dimensional surface Spline(3DSS)gradient model of the main geomagnetic field over the Chinese continent.To improve the precision of the model,we considered the data gap between the ground and satellite data.We compared and analyzed the results of the Taylor polynomial,surface Spline,and CHAOS-6(the CHAMP,?rsted and SAC-C model of Earth’s magnetic field)gradient models.Results showed that the gradients in the south-north and east-west directions of the four models were consistent.The 3DSS model was able to express not only gradients at different altitudes,but also average gradients inside the research area.The two Spline models were able to capture more information on gradient anomalies than were the fitted models.Strong local anomalies were observed in northern Xinjiang,Beijing,and the junction area between Jiangsu and Zhejiang,and the total intensity F decreased whereas the altitude increased.The gradient decreased by 21.69%in the south-north direction and increased by 11.78%in the east-west direction.In addition,the altitude gradient turned from negative to positive while the altitude increased.The Spline model and the two fitted models differed mainly in the field sources they expressed and the modeling theory.

Can preoperative planning using IRIS™three-dimensional anatomical virtual models predict operative findings during robot-assisted partial nephrectomy?

Objective To evaluate the predictive validity of IRIS™(Intuitive Surgical®,Sunnyvale,CA,USA)as a planning tool for robot-assisted partial nephrectomy(RAPN)by assessing the degree of overlap with intraoperative execution.Methods Thirty-one patients scheduled for RAPN by four experienced urologists were enrolled in a prospective study.Prior to surgery,urologists reviewed the IRIS™three-dimensional model on an iphone Operating System(iOS)app and completed a questionnaire outlining their surgical plan including surgical approach,and ischemia technique as well as confidence in executing this plan.Postoperatively,questionnaires assessing the procedural approach,clinical utility,efficiency,and effectiveness of IRIS™were completed.The degree of overlap between the preoperative and intraoperative questionnaires and between the planned approach and actual execution of the procedure was analyzed.Questionnaires were answered on a 5-point Likert scale and scores of 4 or greater were considered positive.Results Mean age was 65.1 years with a mean tumor size of 27.7 mm(interquartile range 17.5-44.0 mm).Hilar tumors consisted of 32.3%;48.4%of patients had R.E.N.A.L.nephrometry scores of 7-9.On preoperative questionnaires,the surgeons reported that in 67.7%cases they were confident that they can perform the procedure successfully,and on intraoperative questionnaires,the surgeons reported that in 96.8%cases IRIS™helped achieve good spatial sensation of the anatomy.There was a high degree of overlap between preoperative and intraoperative questionnaires for the surgical approach,interpreting anatomical details and clinical utility.When comparing plans for selective or off-clamp,the preoperative plan was executed in 90.0%of cases intraoperatively.Conclusion A high degree of overlap between the preoperative surgical approach and intraoperative RAPN execution was found using IRIS™.This is the first study to evaluate the predictive accuracy of IRIS™during RAPN by comparing preoperative plan and intraoperative execution.

General mapping of one-dimensional non-Hermitian mosaic models to non-mosaic counterparts:Mobility edges and Lyapunov exponents

We establish a general mapping from one-dimensional non-Hermitian mosaic models to their non-mosaic counterparts.This mapping can give rise to mobility edges and even Lyapunov exponents in the mosaic models if critical points of localization or Lyapunov exponents of localized states in the corresponding non-mosaic models have already been analytically solved.To demonstrate the validity of this mapping,we apply it to two non-Hermitian localization models:an Aubry-Andre-like model with nonreciprocal hopping and complex quasiperiodic potentials,and the Ganeshan-Pixley-Das Sarma model with nonreciprocal hopping.We successfully obtain the mobility edges and Lyapunov exponents in their mosaic models.This general mapping may catalyze further studies on mobility edges,Lyapunov exponents,and other significant quantities pertaining to localization in non-Hermitian mosaic models.

Three-Dimensional Analytical Modeling of Axial-Flux Permanent Magnet Drivers

In this paper, the axial-flux permanent magnet driver is modeledand analyzed in a simple and novel way under three-dimensional cylindricalcoordinates. The inherent three-dimensional characteristics of the deviceare comprehensively considered, and the governing equations are solved bysimplifying the boundary conditions. The axial magnetization of the sectorshapedpermanent magnets is accurately described in an algebraic form bythe parameters, which makes the physical meaning more explicit than thepurely mathematical expression in general series forms. The parameters of theBessel function are determined simply and the magnetic field distribution ofpermanent magnets and the air-gap is solved. Furthermore, the field solutionsare completely analytical, which provides convenience and satisfactoryaccuracy for modeling a series of electromagnetic performance parameters,such as the axial electromagnetic force density, axial electromagnetic force,and electromagnetic torque. The correctness and accuracy of the analyticalmodels are fully verified by three-dimensional finite element simulations and a15 kW prototype and the results of calculations, simulations, and experimentsunder three methods are highly consistent. The influence of several designparameters on magnetic field distribution and performance is studied and discussed.The results indicate that the modeling method proposed in this papercan calculate the magnetic field distribution and performance accurately andrapidly, which affords an important reference for the design and optimizationof axial-flux permanent magnet drivers.

Design and Realization of Block Level Augmented Reality Three-Dimensional Map

In response to the construction needs of “Real 3D China”, the system structure, functional framework, application direction and product form of block level augmented reality three-dimensional map is designed. Those provide references and ideas for the later large-scale production of augmented reality three-dimensional map. The augmented reality three-dimensional map is produced based on skyline software. Including the map browsing, measurement and analysis and so on, the basic function of three-dimensional map is realized. The special functional module including housing management, pipeline management and so on is developed combining the need of residential quarters development, that expands the application fields of augmented reality three-dimensional map. Those lay the groundwork for the application of augmented reality three-dimensional map. .

A modified method of discontinuity trace mapping using three-dimensional point clouds of rock mass surfaces

This paper presents an automated method for discontinuity trace mapping using three-dimensional point clouds of rock mass surfaces.Specifically,the method consists of five steps:(1)detection of trace feature points by normal tensor voting theory,(2)co ntraction of trace feature points,(3)connection of trace feature points,(4)linearization of trace segments,and(5)connection of trace segments.A sensitivity analysis was then conducted to identify the optimal parameters of the proposed method.Three field cases,a natural rock mass outcrop and two excavated rock tunnel surfaces,were analyzed using the proposed method to evaluate its validity and efficiency.The results show that the proposed method is more efficient and accurate than the traditional trace mapping method,and the efficiency enhancement is more robust as the number of feature points increases.

Three-dimensional computed tomography mapping of posterior malleolar fractures

BACKGROUND Posterior malleolar fractures have been reported to occur in<40%of ankle fractures.AIM To reveal the recurrent patterns and characteristics of posterior malleolar fractures by creating fracture maps of the posterior malleolar fractures through the use of computed tomography mapping.METHODS A consecutive series of posterior malleolar fractures was used to create threedimensional reconstruction images,which were oriented and superimposed to fit an ankle model template by both aligning specific biolandmarks and reducing reconstructed fracture fragments.Fracture lines were found and traced in order to generate an ankle fracture map.RESULTS This study involved 112 patients with a mean age of 49,comprising 32 pronationexternal rotation grade IV fractures and 80 supination-external rotation grade IV fractures according to the Lauge-Hansen classification system.Three-dimensional maps showed that the posterior ankle fracture fragments in the supinationexternal rotation grade IV group were relatively smaller than those in the pronation-external rotation grade IV group after posterior malleolus fracture.In addition,the distribution analyses on posterior malleolus fracture lines indicated that the supination-external rotation grade IV group tended to have higher linear density but more concentrated and orderly distribution fractures compared to the pronation-external rotation grade IV group.CONCLUSION Fracture maps revealed the fracture characteristics and recurrent patterns of posterior malleolar fractures,which might help to improve the understanding of ankle fracture as well as increase opportunities for follow-up research and aid clinical decision-making.

Application of Three-dimensional Laser Scanning Technology in the Teaching Practice of Surveying and Mapping of Ancient Buildings

Based on the study of the application of three-dimensional laser scanning technology in ancient building surveying and mapping,this paper briefly describes the working principle and flow of three-dimensional laser scanning technology.Based on the practical application,this paper puts forward the discussion of related problems and matters needing attention.This has a certain reference significance for the study of new technology in surveying and mapping of ancient buildings.

Ontology mapping based on hidden Markov model

The existing ontology mapping methods mainly consider the structure of the ontology and the mapping precision is lower to some extent. According to statistical theory, a method which is based on the hidden Markov model is presented to establish ontology mapping. This method considers concepts as models, and attributes, relations, hierarchies, siblings and rules of the concepts as the states of the HMM, respectively. The models corresponding to the concepts are built by virtue of learning many training instances. On the basis of the best state sequence that is decided by the Viterbi algorithm and corresponding to the instance, mapping between the concepts can be established by maximum likelihood estimation. Experimental results show that this method can improve the precision of heterogeneous ontology mapping effectively.

Shear behavior of intact granite under thermo-mechanical coupling and three-dimensional morphology of shear-formed fractures

The shear failure of intact rock under thermo-mechanical(TM)coupling conditions is common,such as in enhanced geothermal mining and deep mine construction.Under the effect of a continuous engineering disturbance,shear-formed fractures are prone to secondary instability,posing a severe threat to deep engineering.Although numerous studies regarding three-dimensional(3D)morphologies of fracture surfaces have been conducted,the understanding of shear-formed fractures under TM coupling conditions is limited.In this study,direct shear tests of intact granite under various TM coupling conditions were conducted,followed by 3D laser scanning tests of shear-formed fractures.Test results demonstrated that the peak shear strength of intact granite is positively correlated with the normal stress,whereas it is negatively correlated with the temperature.The internal friction angle and cohesion of intact granite significantly decrease with an increase in the temperature.The anisotropy,roughness value,and height of the asperities on the fracture surfaces are reduced as the normal stress increases,whereas their variation trends are the opposite as the temperature increases.The macroscopic failure mode of intact granite under TM coupling conditions is dominated by mixed tensileeshear and shear failures.As the normal stress increases,intragranular fractures are developed ranging from a local to a global distribution,and the macroscopic failure mode of intact granite changes from mixed tensileeshear to shear failure.Finally,3D morphological characteristics of the asperities on the shear-formed fracture surfaces were analyzed,and a quadrangular pyramid conceptual model representing these asperities was proposed and sufficiently verified.