BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for power...Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for powering induction furnaces, in audio technique. Spectrum of signals sampled by pulse amplitude modulation and output voltage spectrum of the converter with pulse width modulation have similar properties. Spectrum of signals sampled by pulse amplitude modulation contains a harmonic of frequency equal to the frequency of the modulating signal and the harmonics of frequencies equal to the sum of frequency of the modulating signal and multiples of the sampling frequency. The output voltage spectrum of the converter with bipolar pulse width modulation contains harmonic of frequency equal to the frequency of the modulating signal and harmonics of frequencies equal to sum of the frequency of the modulating signal and multiples of the frequency of the carrier signal. It also contains harmonics of frequencies equal to the sum of the multiples of the frequency of the modulating signal and the multiples of the carrier signal. The comparison analysis was carried out for the harmonics of the output voltage of the converter with bipolar pulse width modulation in time domain. The dependency of the amplitudes and frequency spectrum on the wave forms of the carrier signal and modulating signal was shown. Similarity of the output voltage spectrum of the converter and signal spectrum sampled by the pulse width modulation was also shown. Key words： Output voltage converter with bipolar pulse width modulation, spectral analysis, Fourier series, carrier signal, reference signal.展开更多
A large amount of switching loss occurs in the inverter. From this point of view, an inverter design should be optimized for which size and cost will be minimum along with increasing efficiency. The main aim of this p...A large amount of switching loss occurs in the inverter. From this point of view, an inverter design should be optimized for which size and cost will be minimum along with increasing efficiency. The main aim of this paper is the analysis and development of single-phase and three-phase inverter to design with MOSFET and IGBT as power elements by sinusoidal pulse width modulation (SPWM) technique using MATLAB Simulink software and compare their difference with the practical inverter. This work proposes different multilevel stages for the cascaded H-Bridge inverter to enhance the output voltages. Then compare their performance, harmonic distortion, and frequency spectrum. The hardware of an inverter circuit has been developed using the SG3524 microcontroller. The main goal of this design is to generate a sine wave with fewer harmonics, while keeping the cost and complexity of the circuit low. The designed inverter has undergone testing with different AC loads and is primarily intended for low-power applications, as lamps, fans, and chargers. This design aims to provide a reliable and efficient inverter solution for these specific applications.展开更多
In this paper, a vector regulating principle of the phase and amplitude control PAC method for three-phase grid-connected inverters is presented.To solve the problem of heavy inrush current and slow dynamic response w...In this paper, a vector regulating principle of the phase and amplitude control PAC method for three-phase grid-connected inverters is presented.To solve the problem of heavy inrush current and slow dynamic response when system starts up, the starting voltage prediction control and the current feed-forward control are proposed and used, which improve the dynamic performance of the system in the PAC.The experimental results carried out on a three-phase grid-connected inverter proved the validity of the proposed method.展开更多
Multilevel inverters are gaining popularity in high power applications. This paper proposes a new ladder type structure of cascaded three-phase multilevel inverter with reduced number of power semiconductor devices wh...Multilevel inverters are gaining popularity in high power applications. This paper proposes a new ladder type structure of cascaded three-phase multilevel inverter with reduced number of power semiconductor devices which is used to drive the induction motor. The ultimate aim of the paper is to produce multiple output levels with minimum number of semiconductor devices. This paper uses only 11 switches along with 3 diodes and 4 asymmetrical sources to produce an output voltage of 21 levels. The modulation technique plays a major role in commutation of the switches. Here we implement the multicarrier level shifting pulse width modulation technique to produce the commutation signals for the inverter. The proposed multilevel inverter is used to drive the three-phase induction motor. The mathematical modeling of three-phase induction motor is done using Simulink. Furthermore the PI and fuzzy logic controllers are also used to produce the reference waveform of the level shifting technique which in turn produces the commutation signals of the proposed multilevel converter. The controllers are used to control the speed of the induction motor. The effectiveness of the proposed system is proved with the help of simulation. The simulation is performed in MATLAB/Simulink. From the simulation results, it shows that the proposed multilevel inverter works properly to generate the multilevel output waveform with minimum number of semiconductor devices. The PI and fuzzy logic controller performances are evaluated using the results which indicate that with the help of controllers the harmonics has been reduced and the speed control of induction motor is achieved under different loading conditions.展开更多
Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrad...Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrade the performance of the MMC. To improve the harmonic characteristic in the low modulation index, the carrier dynamic overlapping switching frequency optimal pulse width modulation(CDOSFOPWM) method is proposed for the MMC here. The whole modulation index region is divided into three regions: high modulation index region, middle modulation index region, and low modulation index region. The carrier amplitude, carrier overlap ratio, and frequency of triangular carriers are chosen dynamically according to the modulation index region of the modulation signals, to achieve the optimal harmonic characteristic in the whole modulation index region and maintain the switching loss. The number of on-state submodules(SMs) can be calculated by CDOSFOPWM, and the selection of SMs is performed by a reducing switching frequency voltage balancing algorithm. Finally, the proposed method is verified by simulation and experimental results.展开更多
When multiple distributed converters are integrated, the high frequency harmonics will randomly accumulate at the point of common coupling(PCC). This paper proposes a new fast global synchronous discontinuous pulse wi...When multiple distributed converters are integrated, the high frequency harmonics will randomly accumulate at the point of common coupling(PCC). This paper proposes a new fast global synchronous discontinuous pulse width modulation(GSDPWM) method of threephase inverters to effectively attenuate the high frequency current harmonics at PCC. Firstly, the basic principle and the realization method of GSDPWM for three-phase inverters are explained, which can be employed for different modulation types. Then a fast calculation method,which can equally derive the minimized total harmonic distortion(THD) of total current, is proposed to release the calculation burden. Finally, MATLAB simulations and experimental results are presented to verify the performance of GSDPWM.展开更多
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
文摘Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for powering induction furnaces, in audio technique. Spectrum of signals sampled by pulse amplitude modulation and output voltage spectrum of the converter with pulse width modulation have similar properties. Spectrum of signals sampled by pulse amplitude modulation contains a harmonic of frequency equal to the frequency of the modulating signal and the harmonics of frequencies equal to the sum of frequency of the modulating signal and multiples of the sampling frequency. The output voltage spectrum of the converter with bipolar pulse width modulation contains harmonic of frequency equal to the frequency of the modulating signal and harmonics of frequencies equal to sum of the frequency of the modulating signal and multiples of the frequency of the carrier signal. It also contains harmonics of frequencies equal to the sum of the multiples of the frequency of the modulating signal and the multiples of the carrier signal. The comparison analysis was carried out for the harmonics of the output voltage of the converter with bipolar pulse width modulation in time domain. The dependency of the amplitudes and frequency spectrum on the wave forms of the carrier signal and modulating signal was shown. Similarity of the output voltage spectrum of the converter and signal spectrum sampled by the pulse width modulation was also shown. Key words： Output voltage converter with bipolar pulse width modulation, spectral analysis, Fourier series, carrier signal, reference signal.
文摘A large amount of switching loss occurs in the inverter. From this point of view, an inverter design should be optimized for which size and cost will be minimum along with increasing efficiency. The main aim of this paper is the analysis and development of single-phase and three-phase inverter to design with MOSFET and IGBT as power elements by sinusoidal pulse width modulation (SPWM) technique using MATLAB Simulink software and compare their difference with the practical inverter. This work proposes different multilevel stages for the cascaded H-Bridge inverter to enhance the output voltages. Then compare their performance, harmonic distortion, and frequency spectrum. The hardware of an inverter circuit has been developed using the SG3524 microcontroller. The main goal of this design is to generate a sine wave with fewer harmonics, while keeping the cost and complexity of the circuit low. The designed inverter has undergone testing with different AC loads and is primarily intended for low-power applications, as lamps, fans, and chargers. This design aims to provide a reliable and efficient inverter solution for these specific applications.
基金supported by the Shanghai Education Committee Scientific Research Subsidization (Grant No.05AZ30)the Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20060280018)
文摘In this paper, a vector regulating principle of the phase and amplitude control PAC method for three-phase grid-connected inverters is presented.To solve the problem of heavy inrush current and slow dynamic response when system starts up, the starting voltage prediction control and the current feed-forward control are proposed and used, which improve the dynamic performance of the system in the PAC.The experimental results carried out on a three-phase grid-connected inverter proved the validity of the proposed method.
文摘Multilevel inverters are gaining popularity in high power applications. This paper proposes a new ladder type structure of cascaded three-phase multilevel inverter with reduced number of power semiconductor devices which is used to drive the induction motor. The ultimate aim of the paper is to produce multiple output levels with minimum number of semiconductor devices. This paper uses only 11 switches along with 3 diodes and 4 asymmetrical sources to produce an output voltage of 21 levels. The modulation technique plays a major role in commutation of the switches. Here we implement the multicarrier level shifting pulse width modulation technique to produce the commutation signals for the inverter. The proposed multilevel inverter is used to drive the three-phase induction motor. The mathematical modeling of three-phase induction motor is done using Simulink. Furthermore the PI and fuzzy logic controllers are also used to produce the reference waveform of the level shifting technique which in turn produces the commutation signals of the proposed multilevel converter. The controllers are used to control the speed of the induction motor. The effectiveness of the proposed system is proved with the help of simulation. The simulation is performed in MATLAB/Simulink. From the simulation results, it shows that the proposed multilevel inverter works properly to generate the multilevel output waveform with minimum number of semiconductor devices. The PI and fuzzy logic controller performances are evaluated using the results which indicate that with the help of controllers the harmonics has been reduced and the speed control of induction motor is achieved under different loading conditions.
基金supported by National Natural Science Foundation of China(No.51477045)
文摘Modular multilevel converters(MMCs) operate in the low modulation index region in many applications.However, when utilized at the low modulation index region, large harmonics appear in the output voltage, which degrade the performance of the MMC. To improve the harmonic characteristic in the low modulation index, the carrier dynamic overlapping switching frequency optimal pulse width modulation(CDOSFOPWM) method is proposed for the MMC here. The whole modulation index region is divided into three regions: high modulation index region, middle modulation index region, and low modulation index region. The carrier amplitude, carrier overlap ratio, and frequency of triangular carriers are chosen dynamically according to the modulation index region of the modulation signals, to achieve the optimal harmonic characteristic in the whole modulation index region and maintain the switching loss. The number of on-state submodules(SMs) can be calculated by CDOSFOPWM, and the selection of SMs is performed by a reducing switching frequency voltage balancing algorithm. Finally, the proposed method is verified by simulation and experimental results.
文摘When multiple distributed converters are integrated, the high frequency harmonics will randomly accumulate at the point of common coupling(PCC). This paper proposes a new fast global synchronous discontinuous pulse width modulation(GSDPWM) method of threephase inverters to effectively attenuate the high frequency current harmonics at PCC. Firstly, the basic principle and the realization method of GSDPWM for three-phase inverters are explained, which can be employed for different modulation types. Then a fast calculation method,which can equally derive the minimized total harmonic distortion(THD) of total current, is proposed to release the calculation burden. Finally, MATLAB simulations and experimental results are presented to verify the performance of GSDPWM.
