基于磁珠分离的酶联适配体检测痕量蛋白质的新型比色分析方法

以核酸适配体作为高效专一的识别／传感元件，构建了一种新型的磁性分离和特异性捕获的检测方法．两个适配体通过简单的生物素化修饰，利用其与凝血酶不同位点的高亲和力形成夹心结构，其中连接适配体的磁珠可捕获蛋白质，加入另一个适配体及链霉亲和素标记的辣根过氧化物酶后，通过比色法实现靶蛋白检测．该法操作简单，分析时间短，对凝血酶的线性响应范围为10～80nmol／L，检出限为10nmol／L．

Successful treatment of spontaneous rupture of the internal carotid artery in an acute promyelocytic leukemia patient using ultrasound-guided thrombin injection

Spontaneous rupture and bleeding from the carotid artery in acute promyelocytic leukemia （APL）patients leading to pseudoaneurysm （PSA） formation has not been reported. We first successfully treated an APL patient with leli internal carotid artery rupture during chemotherapy leading to PSA using ultrasound-guided thrombin iniection （UGTI）.