Resista nee to anticancer drugs is a major problem in on cology,which causes the failure of an titumor treatme nt.A variety of factors con tribute to drug resistance,in eluding drug efflux and metabolism,tumor cell he...Resista nee to anticancer drugs is a major problem in on cology,which causes the failure of an titumor treatme nt.A variety of factors con tribute to drug resistance,in eluding drug efflux and metabolism,tumor cell heteroge neity,tumor microenvironment stress-induced genetic or epigenetic alterations in the cancer cells and so on.However,how to circumvent this resista nee to improve antican cer efficacy remains to be determined.To circumvent chemotherapeutic resista nee,many reversal agents have been developed,but most of them fail in clinical trials due to severe adverse effects.Recently,several natural products have been reported to augme nt sen sitivity or overcome resista nee of an ticancer chemotherapeutic drugs,including elemene,curcumin,Shenqi Fuzheng Injection(参芪扶正注射液),PHY906,etc.Thus,understand!ng the novel function of Chinese medicine may allow us to develop a promising therapeutic approach to enhance the effects of anticancer strategies and preve nt or overcome their resista nee in the treatment of can cer patie nts.展开更多
针对BOTDR分布式光纤传感技术中背向散射光中布里渊散射信号光的分离提取问题,设计了一种高消光比双通道可调M-Z干涉仪,该干涉仪由两个3 d B耦合器、电动光纤延迟线、偏振控制器及光隔离器构成。使用C波段宽带光源(ASE)对M-Z干涉仪性能...针对BOTDR分布式光纤传感技术中背向散射光中布里渊散射信号光的分离提取问题,设计了一种高消光比双通道可调M-Z干涉仪,该干涉仪由两个3 d B耦合器、电动光纤延迟线、偏振控制器及光隔离器构成。使用C波段宽带光源(ASE)对M-Z干涉仪性能进行了检测。并将脉宽为100 ns,重复频率为20 k Hz的脉冲光入射到长度为5 km的普通单模光纤中,将其产生的背向散射光经过M-Z干涉仪滤波后,通过光谱仪检测其输出的光谱信号。实验结果表明该干涉仪能够实现大范围高精度可调节滤波功能,对瑞利散射光的抑制超过20d B,可以有效地将背向散射光中的布里渊散射光信号分离提取出来。展开更多
Many of the extraction and amino acid analysis methodologies currently employed do not provide complete analysis of all the physiological amino acids and biogenic amines. Extraction procedures frequently employ dilute...Many of the extraction and amino acid analysis methodologies currently employed do not provide complete analysis of all the physiological amino acids and biogenic amines. Extraction procedures frequently employ dilute acid which partially converts gln and asn to glu and asp. A commonly used pre-column derivatizing agent, o-phthalaldehyde, does not react with the imino acids, pro and hydroxypro. The purpose of this investigation was to integrate extraction and analysis procedures into a reliable method for measuring the complete physiological amino acid profiles of fruit and vegetables using HPLC instrumentation commonly available to most laboratories. Water extraction of ground, frozen-thawed tissues effected complete recovery of the physiological amino acids as demonstrated by spiking experiments and tissue combination experiments. HPLC of dabsyl derivatives of the free amino acids allowed their quantification in a selection of fruit and vegetables. Physiological amino acid levels were determined for peach, apple, potato, onion, tomato, bell pepper, broccoli, and seven types of cucurbits. The coefficient of variation for estimation of an amino acid level generally fell in the range of 5% to 7%. Because of marked variability in physiological amino acid content as a result of growing conditions, cultural practices, and inherent cultivar differences, comparisons of results with literature values were not possible.展开更多
A novel data processing procedure for fMRI was suggested in this paper, by which spatial and temporal characteristics of stimuli-induced signal dynamic responses can be investigated simultaneously. First the multitape...A novel data processing procedure for fMRI was suggested in this paper, by which spatial and temporal characteristics of stimuli-induced signal dynamic responses can be investigated simultaneously. First the multitaper spectral estimation was utilized to estimate the spectrum of each voxel; the significance of the line frequency components at the interested frequency was tested to detect the task-related cortex areas; the temporal independent component analysis (tICA) was then applied to the activated voxels to obtain stimuli-induced signal dynamic responses. The advantages of this procedure are: few assumptions are needed for the cerebral hemodynamics and spatial distribution of task-related areas, problems which often appear in tICA analysis of fMRI data, such as the lack of stability, reliability and robustness, are overcome by the suggested method.展开更多
基金Supported by the National Natural Science Foundation of China(No.81672932,81730108,81874380 and 81973635)Zhejiang Provincial Natural Scienee Foundation of China for Distinguished Young Scholars(No.LR18H160001)+3 种基金Zhejia ng Provi nee Scie nee and Tech no logy Project of TCM(No.2019ZZ016)Zhejiang Provinee Medical Science and Tech no logy Project(No.2017RC007)Tale nt Project of Zhejia ng Associati on for Scie nee and Tech no logy(No.2017YCGC002)Key Project of Hangzhou Ministry of Science and Technology(No.20162013A07)。
文摘Resista nee to anticancer drugs is a major problem in on cology,which causes the failure of an titumor treatme nt.A variety of factors con tribute to drug resistance,in eluding drug efflux and metabolism,tumor cell heteroge neity,tumor microenvironment stress-induced genetic or epigenetic alterations in the cancer cells and so on.However,how to circumvent this resista nee to improve antican cer efficacy remains to be determined.To circumvent chemotherapeutic resista nee,many reversal agents have been developed,but most of them fail in clinical trials due to severe adverse effects.Recently,several natural products have been reported to augme nt sen sitivity or overcome resista nee of an ticancer chemotherapeutic drugs,including elemene,curcumin,Shenqi Fuzheng Injection(参芪扶正注射液),PHY906,etc.Thus,understand!ng the novel function of Chinese medicine may allow us to develop a promising therapeutic approach to enhance the effects of anticancer strategies and preve nt or overcome their resista nee in the treatment of can cer patie nts.
文摘针对BOTDR分布式光纤传感技术中背向散射光中布里渊散射信号光的分离提取问题,设计了一种高消光比双通道可调M-Z干涉仪,该干涉仪由两个3 d B耦合器、电动光纤延迟线、偏振控制器及光隔离器构成。使用C波段宽带光源(ASE)对M-Z干涉仪性能进行了检测。并将脉宽为100 ns,重复频率为20 k Hz的脉冲光入射到长度为5 km的普通单模光纤中,将其产生的背向散射光经过M-Z干涉仪滤波后,通过光谱仪检测其输出的光谱信号。实验结果表明该干涉仪能够实现大范围高精度可调节滤波功能,对瑞利散射光的抑制超过20d B,可以有效地将背向散射光中的布里渊散射光信号分离提取出来。
文摘Many of the extraction and amino acid analysis methodologies currently employed do not provide complete analysis of all the physiological amino acids and biogenic amines. Extraction procedures frequently employ dilute acid which partially converts gln and asn to glu and asp. A commonly used pre-column derivatizing agent, o-phthalaldehyde, does not react with the imino acids, pro and hydroxypro. The purpose of this investigation was to integrate extraction and analysis procedures into a reliable method for measuring the complete physiological amino acid profiles of fruit and vegetables using HPLC instrumentation commonly available to most laboratories. Water extraction of ground, frozen-thawed tissues effected complete recovery of the physiological amino acids as demonstrated by spiking experiments and tissue combination experiments. HPLC of dabsyl derivatives of the free amino acids allowed their quantification in a selection of fruit and vegetables. Physiological amino acid levels were determined for peach, apple, potato, onion, tomato, bell pepper, broccoli, and seven types of cucurbits. The coefficient of variation for estimation of an amino acid level generally fell in the range of 5% to 7%. Because of marked variability in physiological amino acid content as a result of growing conditions, cultural practices, and inherent cultivar differences, comparisons of results with literature values were not possible.
基金This work was supported by the National Science Fund for Distinguished Young Scholars(Grant No.60225015)the National Natural Science Foundation of China(Grant Nos.30370416.30100054)+1 种基金the Ministry of Science and Technology of China(Grant No.2001CCA04 100)the Ministry of Education of China(TRAPOYT).
文摘A novel data processing procedure for fMRI was suggested in this paper, by which spatial and temporal characteristics of stimuli-induced signal dynamic responses can be investigated simultaneously. First the multitaper spectral estimation was utilized to estimate the spectrum of each voxel; the significance of the line frequency components at the interested frequency was tested to detect the task-related cortex areas; the temporal independent component analysis (tICA) was then applied to the activated voxels to obtain stimuli-induced signal dynamic responses. The advantages of this procedure are: few assumptions are needed for the cerebral hemodynamics and spatial distribution of task-related areas, problems which often appear in tICA analysis of fMRI data, such as the lack of stability, reliability and robustness, are overcome by the suggested method.
