Species-level Microbiota of Biting Midges and Ticks from Poyang Lake

Objective The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area,namely,Qunlu Practice Base,Peach Blossom Garden,and Huangtong Animal Husbandry,and whether vectors carry any bacterial pathogens that may cause diseases to humans,to provide scientific basis for prospective pathogen discovery and disease prevention and control.Methods Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit(OPU)analysis,we characterized the species-level microbial community structure of two important vector species,biting midges and ticks,including 33 arthropod samples comprising 3,885 individuals,collected around Poyang Lake.Results A total of 662 OPUs were classified in biting midges,including 195 known species and 373 potentially new species,and 618 OPUs were classified in ticks,including 217 known species and 326 potentially new species.Surprisingly,OPUs with potentially pathogenicity were detected in both arthropod vectors,with 66 known species of biting midges reported to carry potential pathogens,including Asaia lannensis and Rickettsia bellii,compared to 50 in ticks,such as Acinetobacter lwoffii and Staphylococcus sciuri.We found that Proteobacteria was the most dominant group in both midges and ticks.Furthermore,the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria.Pantoea sp7 was predominant in biting midges,while Coxiella sp1 was enriched in ticks.Meanwhile,Coxiella spp.,which may be essential for the survival of Haemaphysalis longicornis Neumann,were detected in all tick samples.The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors.Conclusion Biting midges and ticks carry large numbers of known and potentially novel bacteria,and carry a wide range of potentially pathogenic bacteria,which may pose a risk of infection to humans and animals.The microbial communities of midges and ticks tend to be dominated by a few highly abundant bacteria.

Molecular evidence and phylogenetic delineation of spotted fever group Rickettsia species in Amblyomma ticks from cattle in Gauteng and Limpopo Provinces,South Africa

Objective:To determine the prevalence of tick-borne pathogens with a particular focus on Rickettsia spp.in ticks collected from cattle in Gauteng and Limpopo Provinces,South Africa.Methods:A total of 200 ticks were collected from cattle within the Madala livestock,Pretoria,Gauteng Province and in Mankweng Township,Polokwane,Limpopo Province in 2019.The ticks were morphologically identified and processed individually for a total genomic DNA extraction.Specific primers targetting ompA,ompB,and the 17KDa genes were used for a molecular screening and delineation of Rickettsia from the extracted genetic materials using polymerase chain reaction(PCR)technique.PCR amplicons of positive samples were sequenced bidirectionally using the Sanger sequencing method.Sequences generated were processed and analysed using appropriate bioinformatics software.Results:The ticks were morphologically identified as Amblyomma spp.PCR profiling of the genomic DNA samples revealed the presence of the Rickettsia pathogen in 42(21%)of the ticks collected from both Provinces.Out of the genes profiled,14(7%)were positive for 17KDa,42(21%)for ompA and 32(16%)were positive for ompB genes respectively.The nucleotide blast of the sequenced genomes showed high similarity,as high as 100% with other reference Rickettsia(R.)africae in the GenBank.The phylogenetic analysis of the sequences further validated them as R.africae with their characteristic clustering pattern with related reference sequences.Conclusions:There is an abundance of R.africae in Amblyomma ticks collected from cattle in the study areas.This has serious public health implications as individuals who accidentally get infested with the ticks could acquire R.africae.Hence,adequate precautions in terms of sensitization of farmers about the risk and mass mobilization drive to control the vectors in the areas are highly recommended to safeguard public health.

Design and Implementation of an Android GIS Data Collection Application for Students with Non-Programming Experience Using the MIT App Inventor: A Case Study on a Tick Data Collection APP

Learning programming can be a daunting task, especially for students without a programming background or non-computer science students. Most Geographic Information Science (GIS) experts and students come from a non-programming background and the development of applications has mostly been perceived to be a field for computer science students and has mostly been shunned by other non-programming students and courses. Compounded by the availability of Open-Source Data collection tools like the Open Data Kit (ODK), learning programming and android application development is often overlooked by Geo Spatial Scientists. Many studies have been done to identify a suitable application development kit to introduce application development to students in non-programming classes. MIT App inventor is a user-friendly drag-and-drop application development platform that is ideal for introducing application development to students with a non-programming background. In this paper, students doing an undergraduate honors degree in spatial sciences were introduced to the MIT App inventor for the development of a Geo-App capable of collecting spatial data that can be exported into a GIS system like Arc GIS, and QGIS after the data has been collected. The paper investigates the suitability of MIT App Inventor in the development of Geo-Spatial Applications. The application was developed for collaborative mapping of tick species for Mashonaland Central Province in Zimbabwe. The main purpose of the application developed is to act as a tool for veterinary extension workers under the Veterinary Services Department of Mashonaland Central Province allowing them to collect location information for tick monitoring. The application helps expansion workers to moreover outwardly communicate the circumstance at the tick controlling focuses such as plunge tanks by collaboratively making an outline and uploading multimedia records subsequently with an alternative of making calls straight to the veterinary call center in near real-time.

Prevalence of Tick Infestations and Tick-Borne Diseases in Cattle in Cameroon

Ticks induce huge production and economic losses in the livestock industry and create serious environmental, animal and human health problems. The study was carried out to characterize tick species and determine the prevalence of tick infestation and tick-borne diseases in cattle in Cameroon. Tick and blood samples were collected from a total of 742 animals and analyzed to determine the type of tick and haemoparasites using standard procedures. Overall, four tick species namely Amblyomma variegatum (75.09%), Rhipicephalus microplus (19.43%), Rhipicephalus decoloratus (0.88%) and Hyalomma marginatum (0.18%) and six blood disease agents including Anapalasma marginale (11.29%), Ehrlichia ruminantium (3.52%), Babesia bovis (1.32%), Babesia major (0.44%), Anaplasma phagocytophilum (0.29%) and Dermatophilus congolensis (3.37%). Various co-infections were recorded and the predominant associations were Amblyomma variegatum-Rhipicephalus microplus (4.06%) and Amblyomma variegatum-Hyalomma marginatum (0.36%);Anaplasma-Ehrlichia (12%), Anaplasma-Dematophylus (8%), Babesia-Anaplasma-Ehrlichia (14%) and Babesia-Anaplasma-Ehrlichia-Dermato-phylus (5%). Breed, sex, age and locality significantly influenced the rate of tick infestation while locality, breed and age significantly influenced the detection of blood disease agents in the study.

Growth characteristics and pathogenic consequences of predominant entomopathogenic Yukon soil fungi Mortierella alpina and Penicillium expansum,and effectiveness of Met52®,against larvae of the winter tick,Dermacentor albipictus

Water activity-temperature relationships are reported for Yukon soil-isolated strains of Mortierella alpina and Penicillium expansum that are natural enemies to larvae(infestation stage)of the winter tick Dermacentor albipictus.Both fungal strains are psychrotrophs,characterized by low thermal growth ranges,consistent with their occurrence in Yukon soil.In contrast to P.expansum,M.alpina is more temperature sensitive,has a higher requirement,and is more virulent to tick larvae.Researchers and Biological Control Officers should note that for tick population control these M.alpina and P.expansum strains grow and sporulate well under moderate temperature and humid conditions.Our experiments used Met52®Bioinsecticide as a positive control,and this is the first study that demonstrates the effectiveness of this commercial product against winter tick larvae.

Entomopathogenic fungi isolated from Yukon soil against unfed larvae of the winter tick,Dermacentor albipictus

Fungal composition of Yukon soil samples consisted of 29 soil saprobes common to acidified forest,subarctic habitats.All of the fungi identified are psychrotrophs,capable of growth at both 4℃ and 20℃.Of these,a total of 17 fungi were found to be entomopathogenic by winter tick(Dermacentor albipictus)larval bioassays:Aspergillus flavus,Beauveria,namely B.bassiana,Mucor,Paecilomyces yielded>~70%larval mortality and the remainder,mostly Mortierella and Penicillium,averaged~40%larval mortality.Sites where elk(Cervus elaphus canadensis)are observed had an overall greater quantity,and diversity of entomopathogenic soil fungi than sites where elk are not observed.Greater diversity of fungi at elk-inhabited sites is likely a product of warmer soil microclimate(hillsides where elk are seen,lose snow earlier than other areas),animal presence,and increased nutrient enrichment.We conclude that Yukon soils contain fungi that naturally regulate populations of D.albipictus.All fungi are archived at the Wittenberg University Fungus Collection,Springfield,OH,USA.

New tools,new tick-borne diseases?

Tick-borne diseases(TBDs) are a major public health concern that has increased in the past three decades. Nevertheless, emerging or reemerging TBDs may be still misdiagnosed. Molecular biology techniques for the screening of ticks, use of "Omics" approaches and the incorporation of analytical methods such as mass spectrometry or nuclear magnetic resonance, to the study of ticks and their associated pathogens or potential pathogens are promising tools for a more accurate differential diagnosis of TBDs. However, this huge amount of data needs to be carefully interpreted before being incorporated to the routine of clinical practice. In the meantime, a clinical approach and high level of suspicion keep being essential for the diagnosis and proper handling of TBDs.

Molecular Detection of Tick-borne Pathogens in Ticks Collected from Hainan Island,China

Pathogens like bacteria and protozoa,which affect human and animal health worldwide,can be transmitted by vectors like ticks.To investigate the epidemiology and genetic diversity of bacteria and protozoans carried by ticks in Chengmai county of Hainan province,China,285 adult hard ticks belonging to two species[Rhipicephalus sanguineus(sensu lato):183,64.21%and Rhipicephalus microplus:102,35.79%]from dogs,cattle,and goats were collected.Microbial families were identified in these ticks by amplifying the 18 S r RNA,16 S r RNA(rrs),citrate synthase(glt A),and heat shock protein(gro EL)genes.Our data revealed the presence of four recognized species and two Candidatus spp.of Anaplasmataceae and Coxiellaceae.In sum,these data reveal an extensive diversity of Anaplasmataceae bacteria,Coxiellaceae bacteria,Babesiidae,and Hepatozoidae in ticks from Hainan Island,highlighting the need to understand the tickborne pathogen infection in local animals and humans.

Molecular survey of tick-borne pathogens in Ixodid ticks collected from hunted wild animals in Tuscany, Italy

Objective: To determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals. Methods: PCR was executed on DNA extracted from 77 tick pools to detect Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Coxiella burnetii and Rickettsia spp. Results: A total of 432 ticks were collected: 30(6.94%) Haemaphysalis punctata, 72(16.7%) Dermacentor marginatus and 330(76.38%) Ixodes ricinus. For each animal one or two pools of 3 ticks of the same species was constituted. Seventy-seven tick pools were examined by PCR: 58(75.32%) resulted infected and among them 14(18.18%) showed co-infections. In particular, 29(37.66%) pools were positive for Bartonella spp., 23(29.87%) for Anaplasma phagocytophilum, 16(20.78%) for Rickettsia spp., and 5(6.49%) for Borrelia burgdorferi s.l. All samples were negative for Coxiella burnetii. Conclusions: The results demonstrate the presence of several zoonotic tick-borne pathogens in the studied area, and underline the risk of exposure to infections for hunters not only during the outdoor activity, but also when they manipulate hunted animals infested by infected ticks.

Tick-borne pathogens in Iran:A meta-analysis

Objective:Different studies have been performed on the prevalence of tick-borne pathogens in different areas of Iran;however,as far as our knowledge,there is no regional meta-analysis available for consideration and estimation of tick species infected with different pathogens in Iran.Methods:In this review,among different databases,a total of 95 publications were included,and the infection of different tick species to different tick-borne pathogens was determined;furthermore,presence of pathogens(with 95%confidence intervals)in tick vectors was calculated separately for each province,using Comprehensive Meta-Analysis version 2(Biostat,USA).Results:Totally,among all 95 studies,5673 out of 33521 investigated ticks were positive according to different detection methods.Overall estimated presence of pathogens in tick vectors in Iran was 8.6%(95%CI 7.0%-10.6%,P<0.001).Of all 46 species of ticks in 10 genera in Iran,28 species in 9 genera,including Alveonasus,Argas,Boophilus,Dermacentor,Haemaphysalis,Hyalomma,Ixodes,Ornithodoros,and Rhipicephalus were infected with at least 20 pathogens in 10 genera including Aegyptianella,Anaplasma,Babesia,Borrelia,Brucella,Orthonairovirus[CrimeanCongo hemorrhagic fever virus(CCHFV)],Coxiella,Ehrlichia,Rickettsia and Theileria in 26 provinces of Iran.The presence of pathogens in ticks collected in western Iran was more than other regions.Hyalomma anatolicum(20.35%),Rhipicephalus sanguineus(15.00%),and Rhipicephalus bursa(14.08%)were the most prevalent infected ticks for different pathogens.In addition,most literatures were related to CCHFV and Theileria/Babesia spp.Conclusions:Public health and veterinary professionals should be aware of diagnosing possible diseases or outbreaks in vertebrates.

Bacteriome in Ticks Collected from Domestic Livestock in Kenya

Background: Metagenomics approaches are increasingly being utilized as “dipstick” for microbial carriage. In this study, 16S rRNA metagenomics was used to probe for microbial community that resides in the ticks, those they pick from the environment, wildlife and livestock and to identify potential tick borne zoonoses. Methods: Tick DNA from 463 tick pools collected from domestic animals between 2007 and 2008 were amplified with primers that target the 16S rRNA V3-V4 domain and then sequenced on Illumina Miseq platform using 300 cycles version 3 kits. Ticks were pooled according to species and animal from which they were collected. A non-target control was used to track laboratory contaminants. Sequence data were analyzed using Mothur v1.3 pipeline and R v3.3.1 software and taxonomy determined using SILVA rRNA database. Shannon diversity index was used to compute bacterial diversity in each tick species before computing the means. Results: A total of 645 bacteria genera grouped into 27 phyla were identified. Four phyla contributed 97.4% of the 36,973,934 total sequences. Proteobacteria contributed 61.2% of these sequences that tarried to 33.8% genera, compared to 15.9% (23.4% genera) for Firmicutes, 15.6% (20% genera) for Actinobacteria and 4.7% (11.6% genera) for Bacteroidetes. The remaining 23 phyla only contributed 2.6% of the sequence reads (11.2% genera). Amongst the 645 genera, three groups were discernible, with the biggest group comprised commensals/symbionts that contributed 93.6% of the genera, but their individual sequence contribution was very low. Group two comprised genera that are known to contain pathogenic species, with Coxiella contributing 15,445,204 (41.8%) sequences, Corynebacterium (13.6%), Acinetobacter (4.3%), Staphylococcus (3.9%), Bacillus (2.7%) and Porphyromonas (1.6%), Ralstonia (1.5%), Streptococcus (1.3%), Moraxella (1.3%), amongst others. Group three comprised genera known to contain tick borne zoonotic pathogens (TBZ): Rickettsiae, Anaplasma, Francisella, Ehrlichia, Bartonella and Borrelia. Individually the TBZ contributed Amblyomma variegatum carried the least diverse bacteria (mean Shannon diversity index of 2.69 ± 0.92) compared to 3.79 ± 1.10 for A. gemma, 3.71 ± 1.32 for A. hebraeum, 4.15 ± 1.08 for other Amblyomma spp, 3.79 ± 1.37 for Hyalomma truncatum, 3.67 ± 1.38 for other Hyalomma spp, 3.86 ± 1.27 for Rhipicephalus annulatus, 3.56 ± 1.21 for Rh. appendiculatus, and 3.65 ± 1.30 for Rh. Pulchellus, but the difference was not significant (p = 0.443). Conclusion: This study illustrates the utility of 16S rRNA metagenomics in revealing the complexity of bacteria communities that reside and/or transit through the tick having been picked from the environment, livestock and/or wild animals, some with potential to cause zoonoses.

Tick-borne Diseases,Transmission,Host Immune Responses,Diagnosis and Control

Present review article explains tick-borne diseases,transmission,host immune responses,diagnosis and control in relation to climatic variations.Ticks are hematophagous ectoparasites which suck large volumes of blood from livestock and humans.They release large numbers of protozoans,bacteria,rickettsia and viral pathogens during blood feeding and transmit disease pathogens through saliva.Due to heavy blood sucking by ticks animals face significant blood and weight loss that affect their overall health.Due to more severe illness,high economic losses were noted in livestock.This article highlights medically important tick borne diseases in man and livestock,its pathogenesis,diagnosis and treatment methods.The present article emphasizes invasion of hosts,host-pathogen interactions,tick saliva toxin induced host immune responses and biological effects.This article highlighted various tick control methods i.e.physical killing,acaricidal,biological,hormonal,genetic and immunological methods such as administration of protective antibody and vaccines for disease control in human being and his livestock.The authors suggest non-chemical environmentally safe methods for successful control of tick borne diseases to kill cattle,bird and canine invading ticks.

A Reverse-transcription Recombinase-aided Amplification Assay for the Rapid Detection of the Far-Eastern Subtype of Tick-borne Encephalitis Virus

Objective Tick-borne encephalitis virus(TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis(TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease. Methods A reverse-transcription recombinase-aided amplification(RT-RAA) assay was developed. This assay can be completed in one closed tube at 39℃ within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type(WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay. Results The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units(pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay. Conclusion A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.

Tick-size的减小是否改进中国封闭式基金市场的质量?

2003年3月3日沪深证券交易所对封闭式交易基金的最小报价单位进行改革,由过去的“1分钱”改为“0.1分钱”,旨在改善“夹板套利”、“流动性差”等市场弊端.通过对比改制前后衡量证券市场质量的三个指标:买卖价差、市场深度和成交量总体所表现出的状况,验证了“分改厘”政策在某种程度上确实改进封闭式基金市场的交易状况和流动性.

Survey of tick species parasiting domestic ruminants in Ghaemshahr county,Mazandaran province,Iran

Objective:To determine the tick species parasitizing domestic ruminants in Ghaemshahr county in Mazandaran,a Caspian province in the north of Iran.Methods:About 361 sheep, 54 goats and 10 cattle of 18 herds in several villages in Ghaemshahr were inspected for tick infestation.Separated ticks were preserved in 70% alcohol and identified.Results:About 323 ticks(207 female,116 male) were collected,the occurrence of ticks on sheep,goats and cattle were 28.3% .22.2% and 20.0% respectively.The mean number of ticks on each animal was low (3-5 ticks per animal).Rhipicephalus sanguineus,Rhipicephalus bursa.Ixodes ricinus,Boophilus annulalus,Haemaphysalis punctata and Haemaphysalis numidiana were the tick species we found.Rhipicephalus sanguineus were the most abundant species in the study area.The largest number of ticks were generally present from April to July,mostly in animal ears and tails. Ixodes,Boophilus and Haemaphysalis occurred in mountainous areas of Ghaemshahr.whereas Rhipicephalus were present in both mountains and plains of the study area.Conclusions:The result of this study is a survey of tick species from domestic animals in Iran and implication of possible prevention measures for diseases transmitted by ticks.

Tick-borne encephalitis: A review of epidemiology, clinical characteristics, and management

Tick-borne encephalitis is an infection of central nervous system caused by tick-borne encephalitis virus transmitted to humans predominantly by tick bites. During the last few decades the incidence of the disease has been increasing and poses a growing health problem in almost all endemic European and Asian countries. Most cases occur during the highest period of tick activity, in Central Europe mainly from April to November. Tickborne encephalitis is more common in adults than in children. Clinical spectrum of the disease ranges from mild meningitis to severe meningoencephalitis with or without paralysis. Rare clinical manifestations are an abortive form of the disease and a chronic progressive form. A post-encephalitic syndrome, causing long-lasting morbidity that often affects the quality of life develops in up to 50% of patients after acute tick-borne encephalitis. Clinical course and outcome vary by subtype of tick-borne encephalitis virus(the disease caused by the European subtype has milder course and better outcome than the disease caused by Siberian and Far-Easter subtypes), age of patients(increasing age is associated with less favorable outcome), and host genetic factors. Since clinical features and laboratory results of blood and cerebrospinal fluid are nonspecific, the diagnosis must be confirmed by microbiologic findings. The routine laboratory confirmation of the tick-borne encephalitis virus infection is based mainly on the detection of specific Ig M and Ig G antibodies in serum(and cerebrospinal fluid), usually by enzyme-linked immunosorbent assay. There is no specific antiviral treatment for tick-borne encephalitis. Vaccination can effectively prevent the disease and is indicated for persons living in or visiting tick-borne encephalitis endemic areas.

First survey of hard ticks(Acari: Ixodidae) on cattle, sheep and goats in Boeen Zahra and Takistan counties, Iran

Objective:To carry out the distribution survey of hard ticks of livestock in Boeen Zahra and Takistan counties of Qazvin province from April 2010 to September 2010.Methods:Nearly about 2638 sheep, 461 goats and 318 cattle of 38 herds in different geographical areas were searched for tick infestation.Results:The species compositions collected from the livestock of Boeen Zahra and Takistan wereHaemaphysalis concinna(0.63%),Haemaphysalis sulcata(12.66%),Hyalomma anatolicum(3.80%),Hyalomma asiaticum(3.16%),Hyalomma detritum(5.70%),Hyalomma dromedarii(28.48%),Hyalomma marginatum(13.29%),Hyalomma schulzei(1.89%),Rhipicephalus bursa(3.16%) and Rhipicephalus sanguineus(3.16%), and for Takistan's livestock were Hyalomma dromedarii(9.86%),Hyalomma marginatum(13.29%),Hyalomma schulzei(1.89%) and Rhipicephalus sanguineus(3.16%), respectively. Hard ticks compositions in different topographic areas were different.Hyalommaspecies had the most prevalence in the areas.Conclusions:The veterinary and public health investigation of the above species should be taken.

Indian stock market prediction using artificial neural networks on tick data

Introduction:Nowadays,the most significant challenges in the stock market is to predict the stock prices.The stock price data represents a financial time series data which becomes more difficult to predict due to its characteristics and dynamic nature.Case description:Support Vector Machines(SVM)and Artificial Neural Networks(ANN)are widely used for prediction of stock prices and its movements.Every algorithm has its way of learning patterns and then predicting.Artificial Neural Network(ANN)is a popular method which also incorporate technical analysis for making predictions in financial markets.Discussion and evaluation:Most common techniques used in the forecasting of financial time series are Support Vector Machine(SVM),Support Vector Regression(SVR)and Back Propagation Neural Network(BPNN).In this article,we use neural networks based on three different learning algorithms,i.e.,Levenberg-Marquardt,Scaled Conjugate Gradient and Bayesian Regularization for stock market prediction based on tick data as well as 15-min data of an Indian company and their results compared.Conclusion:All three algorithms provide an accuracy of 99.9%using tick data.The accuracy over 15-min dataset drops to 96.2%,97.0%and 98.9%for LM,SCG and Bayesian Regularization respectively which is significantly poor in comparison with that of results obtained using tick data.

莫让Ticking time版块流于形式——以译林新版《英语》四下Unit 7 What’s the matter?为例

相较于旧教材，译林新版《英语》教材在每个单元中增加了专门的评价版块——Tickingtime。结合《义务教育英语课程标准（2011年版）》我们不难发现，编者编写Tickingtime的意图是通过自我评价．引导学生了解自己对本单元所学主要内容的掌握情况，体验进步与成功，反思和调控自己的学习过程。然而在实际教学中，很多教师为了赶教学进度。

Human otoacariasis:Demographic and clinical outcomes in patients with ear-canal ticks and a review of literature

Introduction:Otoacariasis is a rare infestation of the ear canal,which affects the quality of life especially in rural areas.Different types of ticks and mites may cause otoacariasis.Although treatment of otoacariasis is simple,diseases transmitted through ticks and mites should be considered during diagnosis and treatment.Both local and systemic signs and symptoms of such diseases should be followed up.A literature review was conducted in PubMed using the following terms: 'otoacariasis,' 'ticks,' 'mites,' and 'outer ear canal infestations.' Demographic,radiologic,and treatment options were discussed.Treatment hints and pitfalls were also discussed with the literature review.Conclusion:In this paper,we describe otoacariasis in humans and discuss the appropriate interventions.