This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers....This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)O.Compared with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary Feincreased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.展开更多
Considering the phase behaviors in condensate gas reservoirs and the oil-gas two-phase linear flow and boundary-dominated flow in the reservoir,a method for predicting the relationship between oil saturation and press...Considering the phase behaviors in condensate gas reservoirs and the oil-gas two-phase linear flow and boundary-dominated flow in the reservoir,a method for predicting the relationship between oil saturation and pressure in the full-path of tight condensate gas well is proposed,and a model for predicting the transient production from tight condensate gas wells with multiphase flow is established.The research indicates that the relationship curve between condensate oil saturation and pressure is crucial for calculating the pseudo-pressure.In the early stage of production or in areas far from the wellbore with high reservoir pressure,the condensate oil saturation can be calculated using early-stage production dynamic data through material balance models.In the late stage of production or in areas close to the wellbore with low reservoir pressure,the condensate oil saturation can be calculated using the data of constant composition expansion test.In the middle stages of production or when reservoir pressure is at an intermediate level,the data obtained from the previous two stages can be interpolated to form a complete full-path relationship curve between oil saturation and pressure.Through simulation and field application,the new method is verified to be reliable and practical.It can be applied for prediction of middle-stage and late-stage production of tight condensate gas wells and assessment of single-well recoverable reserves.展开更多
This paper presents a novel view of the impact of electron collision off-axis positions on the dynamic properties and relativistic nonlinear Thomson inverse scattering of excited electrons within tightly focused, circ...This paper presents a novel view of the impact of electron collision off-axis positions on the dynamic properties and relativistic nonlinear Thomson inverse scattering of excited electrons within tightly focused, circularly polarized laser pulses of varying intensities. We examine the effects of the transverse ponderomotive force, specifically how the deviation angle and speed of electron motion are affected by the initial off-axis position of the electron and the peak amplitude of the laser pulse. When the laser pulse intensity is low, an increase in the electron's initial off-axis distance results in reduced spatial radiation power, improved collimation, super-continuum phenomena generation, red-shifting of the spectrum's harmonic peak, and significant symmetry in the radiation radial direction. However, in contradiction to conventional understandings,when the laser pulse intensity is relatively high, the properties of the relativistic nonlinear Thomson inverse scattering of the electron deviate from the central axis, changing direction in opposition to the aforementioned effects. After reaching a peak, these properties then shift again, aligning with the previous direction. The complex interplay of these effects suggests a greater nuance and intricacy in the relationship between laser pulse intensity, electron position, and scattering properties than previously thought.展开更多
The Daqing exploration area in the northern Songliao Basin has great potential for unconventional oil and gas resources,among which the total resources of tight oil alone exceed 109 t and is regarded as an important r...The Daqing exploration area in the northern Songliao Basin has great potential for unconventional oil and gas resources,among which the total resources of tight oil alone exceed 109 t and is regarded as an important resource base of Daqing oilfield.After years of exploration in the Qijia area,Songliao Basin,NE China,tight oil has been found in the Upper Cretaceous Qingshankou Formation.To work out tight oil’s geological characteristics,taking tight oil in Gaotaizi oil layers of the Upper Cretaceous Qingshankou Formation in northern Songliao Basin as an example,this paper systematically analyzed the geological characteristics of unconventional tight oil in Gao3 and Gao4 layers of the Qijia area,based on the data of the geological survey,well drilling journey,well logging,and test.It is that three sets of hydrocarbon source rocks(K2qn1,K2qn2+3,and K2n1)develop in the examined area,and exhibit excellent type I and II kerogens,high organic matter abundance,and moderate maturity.The reservoir is generally composed of thin-bedded mudstone,siltstone,and sandstone,and presents poor porosity(average 8.5 vol.%)and air permeability(average 4 mD).The main reservoir space primarily includes intergranular pores,secondary soluble pores,and intergranular soluble pores.Three types of orifice throats were identified,namely fine throat,extra-fine throat,and micro-fine throat.The siltstone is generally oil-bearing,the reservoirs with slime and calcium become worse oil-bearing,and the mudstone has no obvious oil-bearing characteristics.The brittleness indices of the sandstone in the tight oil reservoir range from 40%to 60%,and those of the mudstone range from 40%to 45%,indicating a better brittleness of the tight oil reservoir.Based on the study of typical core hole data,this paper gives a comprehensive evaluation of the properties of the tight oil and establishes a tight oil single well composite bar chart as well as the initial evaluation system with the core of properties in the tight oil reservoir.This study has theoretical guiding significance and practical application value for tight oil exploration and evaluation in the Qijia area.展开更多
A seepage-geomechanical coupled embedded fracture flow model has been established for multi-field coupled simulation in tight oil reservoirs,revealing the patterns of change in pressure field,seepage field,and stress ...A seepage-geomechanical coupled embedded fracture flow model has been established for multi-field coupled simulation in tight oil reservoirs,revealing the patterns of change in pressure field,seepage field,and stress field after long-term water injection in tight oil reservoirs.Based on this,a technique for enhanced oil recovery(EOR)combining multi-field reconstruction and combination of displacement and imbibition in tight oil reservoirs has been proposed.The study shows that after long-term water flooding for tight oil development,the pressure diffusion range is limited,making it difficult to establish an effective displacement system.The variation in geostress exhibits diversity,with the change in horizontal minimum principal stress being greater than that in horizontal maximum principal stress,and the variation around the injection wells being more significant than that around the production wells.The deflection of geostress direction around injection wells is also large.The technology for EOR through multi-field reconstruction and combination of displacement and imbibition employs water injection wells converted to production and large-scale fracturing techniques to restructure the artificial fracture network system.Through a full lifecycle energy replenishment method of pre-fracturing energy supplementation,energy increase during fracturing,well soaking for energy storage,and combination of displacement and imbibition,it effectively addresses the issue of easy channeling of the injection medium and difficult energy replenishment after large-scale fracturing.By intensifying the imbibition effect through the coordination of multiple wells,it reconstructs the combined system of displacement and imbibition under a complex fracture network,transitioning from avoiding fractures to utilizing them,thereby improving microscopic sweep and oil displacement efficiencies.Field application in Block Yuan 284 of the Huaqing Oilfield in the Ordos Basin has demonstrated that this technology increases the recovery factor by 12 percentage points,enabling large scale and efficient development of tight oil.展开更多
Based on the typical dissection of various onshore tight oil fields in China,the tight oil migration and accumulation mechanism and enrichment-controlling factors in continental lake basins are analyzed through nuclea...Based on the typical dissection of various onshore tight oil fields in China,the tight oil migration and accumulation mechanism and enrichment-controlling factors in continental lake basins are analyzed through nuclear magnetic resonance(NMR)displacement physical simulation and Lattice Boltzmann numerical simulation by using the samples of source rock,reservoir rock and crude oil.In continental lake basins,the dynamic forces driving hydrocarbon generation and expulsion of high-quality source rocks are the foundational power that determines the charging efficiency and accumulation effect of tight oil,the oil migration resistance is a key element that influences the charging efficiency and accumulation effect of tight oil,and the coupling of charging force with pore-throat resistance in tight reservoir controls the tight oil accumulation and sweet spot enrichment.The degree of tight oil enrichment in continental lake basins is controlled by four factors:source rock,reservoir pore-throat size,anisotropy of reservoir structure,and fractures.The high-quality source rocks control the near-source distribution of tight oil,reservoir physical properties and pore-throat size are positively correlated with the degree of tight oil enrichment,the anisotropy of reservoir structure reveals that the parallel migration rate is the highest,and intralayer fractures can improve the migration and accumulation efficiency and the oil saturation.展开更多
Countercurrent imbibition is an important mechanism for tight oil recovery,that is,water imbibes spontaneously from the fracture into the porous matrix while oil flows reversely into the fracture.Its significance over...Countercurrent imbibition is an important mechanism for tight oil recovery,that is,water imbibes spontaneously from the fracture into the porous matrix while oil flows reversely into the fracture.Its significance over cocurrent imbibition and forced imbibition is highlighted when permeability reduces.We used the computed tomography(CT)scanning to measure the one-dimensional evolution of water saturation profile and countercurrent imbibition distance(CID)at different fluid pressures,initial water saturations,and permeability.Surprisingly,experiments show that CID evolution for tight reservoir cores dramatically deviates from the classical diffusive rule(i.e.,evolutes proportional to square root of time,t^(0.5)).At early stage,CID extends faster than t^(0.5)(super-diffusive);while at late stage,CID extends much slower than t^(0.5)(sub-diffusive).After tens of hours,the CID change becomes too slow to be practically efficient for tight oil recovery.This research demonstrates that this deviation from classic theory is a result of(1)a much longer characteristic capillary length than effective invasion depth,which eliminates full development of a classical displacement front;and(2)non-zero flow at low water saturation,which was always neglected for conventional reservoir and is amplified in sub-mili-Darcy rocks.To well depict the details of the imbibition front in this situation,we introduce non-zero wetting phase fluidity at low saturation into classical countercurrent imbibition model and conduct numerical simulations,which successfully rationalizes the non-diffusive behavior and fits experimental data.Our data and theory imply an optimum soaking time in tight oil recovery by countercurrent imbibition,beyond which increasing exposed fracture surface area becomes a more efficient enhanced oil recovery(EOR)strategy than soaking for longer time.展开更多
Background:Bacillus cereus is an important pathogen that causes human food poisoning,specifically diarrhea and vomiting.B.cereus can also induce mastitis in dairy cows and has a strong survival ability in milk,as it c...Background:Bacillus cereus is an important pathogen that causes human food poisoning,specifically diarrhea and vomiting.B.cereus can also induce mastitis in dairy cows and has a strong survival ability in milk,as it cannot be inactivated by high-temperature short-time pasteurization.Therefore,B.cereus can enter the market through pasteurized milk and other dairy products,imposing enormous hidden dangers on food safety and human health.Results:In this study,B.cereus 2101(BC)was isolated from milk samples of cows with mastitis.BC grew rapidly with strong hemolysis,making it difficult to prevent mastitis and ensure food security.MAC-T cells were treated with BC and/or Lactobacillus rhamnosus GR-1(LGR-1).Pretreatment with LGR-1 protected the integrity of tight junctions and the expression of zonula occludens-1(ZO-1)and occludin destroyed by BC.Furthermore,LGR-1 pretreatment reduced the expression of NOD-like receptor family member pyrin domain-containing protein 3(NLRP3),caspase recruitment and activation domain(ASC),Caspase-1 p20,gasdermin D(GSDMD)p30,inflammatory factors(interleukin(IL)-1βand IL-18),and cell death induced by BC.Moreover,LGR-1 pretreatment reduced NLRP3 inflammasome activity and increased expressions of ZO-1 and occludin induced by lipopolysaccharides(LPS)+ATP stimulation.MAC-T cells were transfected with NLRP3 si RNA or MCC950 and/or treated with BC and/or LGR-1.NLRP3-si RNA transfection and MCC950 attenuated BC-induced NLRP3 inflammasome activity.Expression of inflammatory cytokines and cell death suggested that the inflammatory pathway might play an important role in the induction of the NLRP3 inflammasome by BC and the protection of LGR-1.Conclusions:These results suggest that LGR-1 might be a probiotic alternative to antibiotics and could be administered to prevent mastitis in dairy cows,thus ensuring food security.展开更多
Due to the poor physical properties of tight reservoirs,CO_(2) huff-n-puff(HNP)is considered a potential enhanced oil recovery(EOR)method after primary depletion.Optimization plays a critical role in the effective imp...Due to the poor physical properties of tight reservoirs,CO_(2) huff-n-puff(HNP)is considered a potential enhanced oil recovery(EOR)method after primary depletion.Optimization plays a critical role in the effective implementation of CO_(2) huff-n-puff.But the optimization requires a good understanding of the EOR mechanisms.In this work,the spatial distribution of oil saturation under different experimental conditions was analyzed by the NMR method to further discuss the HNP mechanisms.According to the variation of 1D frequency signal amplitude,we divided the core into the hardly movable area and movable area,the region with the obvious signal decline was defined as the movable area,and the hardly movable area was the region with limited signal decline.Based on that the recovery characteristics of different scenarios were evaluated.Firstly,the necessity of the soaking stage was studied,where three scenarios with different soaking times were carried out.Secondly,the injection pressure was adjusted to investigate the effect of the pressure gradient.The T_(2) spectra show that soaking has significantly improved the production of crude oil in small pores,and higher oil recovery in a single cycle is observed,but it is lower when the elapsed time(total operation time)is the same.31.03% of oil can be recovered after 3 cycles HNP,which increases to 33.8% and 37.06% for the 4 cycles and 6 cycles cases.As the pressure gradient increases,more oil is removed out of the matrix,and the oil in the deep part of the reservoir can be effectively recovered.During the CO_(2) huff-n-puff process,the oil distributions are similar to the solution gas drive,the residual oil is distributed at the close end of the core and the range that the oil can be efficiently recovered is limited.展开更多
Tight conglomerate reservoirs are featured with extremely low permeability,strong heterogeneity and poor water injectivity.CO_(2) huff-n-puff has been considered a promising candidate to enhance oil recovery in tight ...Tight conglomerate reservoirs are featured with extremely low permeability,strong heterogeneity and poor water injectivity.CO_(2) huff-n-puff has been considered a promising candidate to enhance oil recovery in tight reservoirs,owing to its advantages in reducing oil viscosity,improving mobility ratio,quickly replenishing formation pressure,and potentially achieving a miscible state.However,reliable inhouse laboratory evaluation of CO_(2) huff-n-puff in natural conglomerate cores is challenging due to the inherent high formation pressure.In this study,we put forward an equivalent method based on the similarity of the miscibility index and Grashof number to acquire a lab-controllable pressure that features the flow characteristics of CO_(2) injection in a tight conglomerate reservoir.The impacts of depletion degree,pore volume injection of CO_(2) and soaking time on ultimate oil recovery in tight cores from the Mahu conglomerate reservoir were successfully tested at an equivalent pressure.Our results showed that oil recovery decreased with increased depletion degree while exhibiting a non-monotonic tendency(first increased and then decreased)with increased CO_(2) injection volume and soaking time.The lower oil recoveries under excess CO_(2) injection and soaking time were attributed to limited CO_(2) dissolution and asphaltene precipitation.This work guides secure and reliable laboratory design of CO_(2) huff-n-puff in tight reservoirs with high formation pressure.展开更多
The chronic complications of diabetes mellitus constitute a major public health problem.For example,diabetic eye diseases are the most important cause of blindness,and diabetic nephropathy is the most frequent cause o...The chronic complications of diabetes mellitus constitute a major public health problem.For example,diabetic eye diseases are the most important cause of blindness,and diabetic nephropathy is the most frequent cause of chronic kidney disease worldwide.The cellular and molecular mechanisms of these chronic complications are still poorly understood,preventing the development of effective treatment strategies.Tight junctions(TJs)are epithelial intercellular junctions located at the most apical region of cell-cell contacts,and their main function is to restrict the passage of molecules through the paracellular space.The TJs consist of over 40 proteins,and the most important are occludin,claudins and the zonula occludens.Accumulating evidence suggests that TJ disruption in different organs,such as the brain,nerves,retina and kidneys,plays a fundamental pathophysiological role in the development of chronic complications.Increased permeability of the blood-brain barrier and the blood-retinal barrier has been demonstrated in diabetic neuropathy,brain injury and diabetic retinopathy.The consequences of TJ disruption on kidney function or progression of kidney disease are currently unknown.In the present review,we highlighted the molecular events that lead to barrier dysfunction in diabetes.Further investigation of the mechanisms underlying TJ disruption is expected to provide new insights into therapeutic approaches to ameliorate the chronic complications of diabetes mellitus.展开更多
Unconventional gas in the Sichuan Basin mainly includes shale gas and tight gas.The development of shale gas is mainly concentrated in the Ordovician Wufeng Formation-Silurian Longmaxi Formation,but has not made any s...Unconventional gas in the Sichuan Basin mainly includes shale gas and tight gas.The development of shale gas is mainly concentrated in the Ordovician Wufeng Formation-Silurian Longmaxi Formation,but has not made any significant breakthrough in the Cambrian Qiongzhusi Formation marine shale regardless of exploration efforts for years.The commercial development of tight sandstone gas is mainly concentrated in the Jurassic Shaximiao Formation,but has not been realized in the widespread and thick Triassic Xujiahe Formation.Depending on the geological characteristics of the Qiongzhusi Formation and Xujiahe Formation,the feedback of old wells was analyzed.Then,combining with the accumulation mechanisms of con-ventional gas and shale gas,as well as the oil/gas shows during drilling,changes in production and pressure during develop-ment,and other characteristics,it was proposed to change the exploration and development strategy from source and reservoir exploration to carrier beds exploration.With the combination of effective source rock,effective carrier beds and effective sand-stone or shale as the exploration target,a model of unconventional gas accumulation and enrichment in carrier beds was built.Under the guidance of this study,two significant results have been achieved in practice.First,great breakthrough was made in exploration of the silty shale with low organic matter abundance in the Qiongzhusi Formation,which breaks the traditional approach to prospect shale gas only in organic-rich black shales and realizes a breakthrough in new areas,new layers and new types of shale gas and a transformation of exploration and development of shale gas from single-layer system,Longmaxi For-mation,to multi-layer system in the Sichuan Basin.Second,exploration breakthrough and high-efficient development were re-alized for difficult-to-produce tight sandstone gas reserves in the Xujiahe Formation,which helps address the challenges of low production and unstable production of fracture zones in the Xujiahe Formation,promote the transformation of tight sandstone gas from reserves without production to effective production,and enhance the exploration and development potential of tight sandstonegas.展开更多
In the second member of the Upper Triassic Xujiahe Formation(T_(3)x_(2))in the Xinchang area,western Sichuan Basin,only a low percent of reserves has been recovered,and the geological model of gas reservoir sweet spot...In the second member of the Upper Triassic Xujiahe Formation(T_(3)x_(2))in the Xinchang area,western Sichuan Basin,only a low percent of reserves has been recovered,and the geological model of gas reservoir sweet spot remains unclear.Based on a large number of core,field outcrop,test and logging-seismic data,the T_(3)x_(2) gas reservoir in the Xinchang area is examined.The concept of fault-fold-fracture body(FFFB)is proposed,and its types are recognized.The main factors controlling fracture development are identified,and the geological models of FFFB are established.FFFB refers to faults,folds and associated fractures reservoirs.According to the characteristics and genesis,FFFBs can be divided into three types:fault-fracture body,fold-fracture body,and fault-fold body.In the hanging wall of the fault,the closer to the fault,the more developed the effective fractures;the greater the fold amplitude and the closer to the fold hinge plane,the more developed the effective fractures.Two types of geological models of FFFB are established:fault-fold fracture,and matrix storage and permeability.The former can be divided into two subtypes:network fracture,and single structural fracture,and the later can be divided into three subtypes:bedding fracture,low permeability pore,and extremely low permeability pore.The process for evaluating favorable FFFB zones was formed to define favorable development targets and support the well deployment for purpose of high production.The study results provide a reference for the exploration and development of deep tight sandstone oil and gas reservoirs in China.展开更多
Spontaneous imbibition is an important phenomenon in tight reservoirs.The existence of a large number of fractures and micro-nano pores is the key factor affecting the spontaneous imbibition of tight reservoirs.In thi...Spontaneous imbibition is an important phenomenon in tight reservoirs.The existence of a large number of fractures and micro-nano pores is the key factor affecting the spontaneous imbibition of tight reservoirs.In this study,based on high-pressure mercury injection and nuclear magnetic resonance experiments,the pore distribution of tight sandstone is described.The influence of fractures,core porosity and permeability,and surfactants on the spontaneous imbibition of tight sandstone are studied by physical fracturing,interfacial tension test,wettability test and imbibition experiments.The results show that:the pore radius of tight sandstone is concentrated in 0.01-1 mm.Fractures can effectively reduce the oil drop adsorption on the core surface,enhancing the imbibition recovery of the tight sandstone with an increase of about 10%.As the number of fractures increases,the number of oil droplets adsorbed on the core surface decrease and the imbibition rate increases.The imbibition recovery increases with the increase in pore connectivity,while the imbibition rate increases with the increases in core porosity and permeability.The surfactant can improve the core water wettability and reduce the oilwater interfacial tension,reducing the adsorption of oil droplets on the core surface,and improving the core imbibition recovery with an increase of about 15%.In a word,the existence of fractures and surfactants can enhance the pore connectivity of the reservoir,reduce the adsorption of oil droplets on the core surface,and improve the imbibition rate and recovery rate of the tight oil reservoir.展开更多
Fuzzy-ball working fluids(FBWFs)have been successfully applied in different development phases of tight reservoirs.Field reports revealed that FBWFs satisfactorily met all the operational and reservoir damage control ...Fuzzy-ball working fluids(FBWFs)have been successfully applied in different development phases of tight reservoirs.Field reports revealed that FBWFs satisfactorily met all the operational and reservoir damage control requirements during their application.However,the damage-control mechanisms and degree of formation damage caused by fuzzy-ball fluids have not been investigated in lab-scale studies so far.In this study,the degree of fuzzy-ball-induced damage in single-and double-layer reservoirs was evaluated through core flooding experiments that were based on permeability and flow rate indexes.Additionally,its damage mechanisms were observed via scanning electron microscope and energy-dispersive spectroscopy tests.The results show that:(1)For single-layer reservoirs,the FBWF induced weak damage on coals and medium-to-weak damage on sandstones,and the difference of the damage in permeability or flow rate index on coals and sandstones is below 1%.Moreover,the minimum permeability recovery rate was above 66%.(2)For double-layer commingled reservoirs,the flow rate index revealed weak damage and the overall damage in double-layer was lower than the single-layer reservoirs.(3)There is no significant alteration in the microscopic structure of fuzzy-ball saturated cores with no evidence of fines migration.The dissolution of lead and sulfur occurred in coal samples,while tellurium in sandstone,aluminum,and magnesium in carbonate.However,the precipitation of aluminum,magnesium,and sodium occurred in sandstone but no precipitates found in coal and carbonate.The temporal plugging and dispersion characteristics of the FBWFs enable the generation of reservoir protection layers that will minimize formation damage due to solid and fluid invasion.展开更多
In this study,X-ray diffraction,N_(2)adsorption(N_(2)A),and mercury intrusion(MI)experiments were used to investigate the influence of acid treatment on pore structure and fractal characterization of tight sandstones....In this study,X-ray diffraction,N_(2)adsorption(N_(2)A),and mercury intrusion(MI)experiments were used to investigate the influence of acid treatment on pore structure and fractal characterization of tight sandstones.The results showed that acid treatment generated a certain number of ink-bottle pores in fine sandstone,aggravated the ink-bottle effect in the sandy mudstone,and transformed some smaller pores into larger ones.After the acid treatment,both the pore volume in the range of 2–11 nm and 0.271–8μm for the fine sandstone and the entire pore size range for the sandy mudstone significantly increased.The dissolution of sandstone cement causes the fine sandstone particles to fall off and fill the pores;the porosity increased at first but then decreased with acid treatment time.The fractal dimension obtained using the Frenkel-Halsey-Hill model was positively correlated with acid treatment time.However,the total fractal dimensions obtained by MI tests showed different changes with acid treatment time in fine sandstone and sandy mudstone.These results provide good guiding significance for reservoir acidification stimulation.展开更多
During the operation of compressed air storage energy system,the rapid change of air pressure in a cavern will cause drastic changes in air density and permeability coefficient of sealing layer.To calculate and proper...During the operation of compressed air storage energy system,the rapid change of air pressure in a cavern will cause drastic changes in air density and permeability coefficient of sealing layer.To calculate and properly evaluate air tightness of polymer sealing caverns,the air-pressure-related air density and permeability must be considered.In this context,the high-pressure air penetration in the polymer sealing layer is studied in consideration of thermodynamic change of the cavern structure during the system operation.The air tightness model of compressed air storage energy caverns is then established.In the model,the permeability coefficient and air density of sealing layer vary with air pressure,and the effectiveness of the model is verified by field data in two test caverns.Finally,a compressed air storage energy cavern is taken as an example to understand the air tightness.The air leakage rate in the caverns is larger than that using air-pressure-independent permeability coefficient and air density,which is constant and small in the previous leakage rate calculation.Under the operating pressure of 4.5-10 MPa,the daily air leakage in the compressed air storage energy cavern of Yungang Mine with high polymer butyl rubber as the sealing material is 0.62%,which can meet the sealing requirements of compressed air storage energy caverns.The air tightness of the polymer sealing cavern is mainly affected by the cavern operating pressure,injected air temperature,cavern radius,and sealing layer thickness.The cavern air leakage rate will be decreased to reduce the cavern operating pressure the injection air temperature,or the cavern radius and sealing layer thickness will be increased.展开更多
基金supported by the National Natural Science Foundation of China(31501977)the Sichuan Provincial Key R&D Project China(22ZDYF0194)the Double World-Class Project of Southwest Minzu University China(XM2023010)。
文摘This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)O.Compared with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary Feincreased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.
基金Supported by National Natural Science Foundation of China(52104049)Young Elite Scientist Sponsorship Program by BAST(BYESS2023262)Science Foundation of China University of Petroleum,Beijing(2462022BJRC004).
文摘Considering the phase behaviors in condensate gas reservoirs and the oil-gas two-phase linear flow and boundary-dominated flow in the reservoir,a method for predicting the relationship between oil saturation and pressure in the full-path of tight condensate gas well is proposed,and a model for predicting the transient production from tight condensate gas wells with multiphase flow is established.The research indicates that the relationship curve between condensate oil saturation and pressure is crucial for calculating the pseudo-pressure.In the early stage of production or in areas far from the wellbore with high reservoir pressure,the condensate oil saturation can be calculated using early-stage production dynamic data through material balance models.In the late stage of production or in areas close to the wellbore with low reservoir pressure,the condensate oil saturation can be calculated using the data of constant composition expansion test.In the middle stages of production or when reservoir pressure is at an intermediate level,the data obtained from the previous two stages can be interpolated to form a complete full-path relationship curve between oil saturation and pressure.Through simulation and field application,the new method is verified to be reliable and practical.It can be applied for prediction of middle-stage and late-stage production of tight condensate gas wells and assessment of single-well recoverable reserves.
基金Project supported by the National Natural Science Foundation of China (Grant Nos.10947170/A05 and 11104291)the Natural Science Fund for Colleges and Universities in Jiangsu Province (Grant No.10KJB140006)+2 种基金the Natural Sciences Foundation of Shanghai (Grant No.11ZR1441300)the Natural Science Foundation of Nanjing University of Posts and Telecommunications (Grant No.NY221098)the Jiangsu Qing Lan Project for their sponsorship。
文摘This paper presents a novel view of the impact of electron collision off-axis positions on the dynamic properties and relativistic nonlinear Thomson inverse scattering of excited electrons within tightly focused, circularly polarized laser pulses of varying intensities. We examine the effects of the transverse ponderomotive force, specifically how the deviation angle and speed of electron motion are affected by the initial off-axis position of the electron and the peak amplitude of the laser pulse. When the laser pulse intensity is low, an increase in the electron's initial off-axis distance results in reduced spatial radiation power, improved collimation, super-continuum phenomena generation, red-shifting of the spectrum's harmonic peak, and significant symmetry in the radiation radial direction. However, in contradiction to conventional understandings,when the laser pulse intensity is relatively high, the properties of the relativistic nonlinear Thomson inverse scattering of the electron deviate from the central axis, changing direction in opposition to the aforementioned effects. After reaching a peak, these properties then shift again, aligning with the previous direction. The complex interplay of these effects suggests a greater nuance and intricacy in the relationship between laser pulse intensity, electron position, and scattering properties than previously thought.
基金funded by the shale oil and gas geological survey project in Quemoco sag,Qiangtang Basin of China Geological Survey(DD20221855,DD20230315).
文摘The Daqing exploration area in the northern Songliao Basin has great potential for unconventional oil and gas resources,among which the total resources of tight oil alone exceed 109 t and is regarded as an important resource base of Daqing oilfield.After years of exploration in the Qijia area,Songliao Basin,NE China,tight oil has been found in the Upper Cretaceous Qingshankou Formation.To work out tight oil’s geological characteristics,taking tight oil in Gaotaizi oil layers of the Upper Cretaceous Qingshankou Formation in northern Songliao Basin as an example,this paper systematically analyzed the geological characteristics of unconventional tight oil in Gao3 and Gao4 layers of the Qijia area,based on the data of the geological survey,well drilling journey,well logging,and test.It is that three sets of hydrocarbon source rocks(K2qn1,K2qn2+3,and K2n1)develop in the examined area,and exhibit excellent type I and II kerogens,high organic matter abundance,and moderate maturity.The reservoir is generally composed of thin-bedded mudstone,siltstone,and sandstone,and presents poor porosity(average 8.5 vol.%)and air permeability(average 4 mD).The main reservoir space primarily includes intergranular pores,secondary soluble pores,and intergranular soluble pores.Three types of orifice throats were identified,namely fine throat,extra-fine throat,and micro-fine throat.The siltstone is generally oil-bearing,the reservoirs with slime and calcium become worse oil-bearing,and the mudstone has no obvious oil-bearing characteristics.The brittleness indices of the sandstone in the tight oil reservoir range from 40%to 60%,and those of the mudstone range from 40%to 45%,indicating a better brittleness of the tight oil reservoir.Based on the study of typical core hole data,this paper gives a comprehensive evaluation of the properties of the tight oil and establishes a tight oil single well composite bar chart as well as the initial evaluation system with the core of properties in the tight oil reservoir.This study has theoretical guiding significance and practical application value for tight oil exploration and evaluation in the Qijia area.
基金Supported by the Joint Fund Project of the National Natural Science Foundation of China(U22B2075).
文摘A seepage-geomechanical coupled embedded fracture flow model has been established for multi-field coupled simulation in tight oil reservoirs,revealing the patterns of change in pressure field,seepage field,and stress field after long-term water injection in tight oil reservoirs.Based on this,a technique for enhanced oil recovery(EOR)combining multi-field reconstruction and combination of displacement and imbibition in tight oil reservoirs has been proposed.The study shows that after long-term water flooding for tight oil development,the pressure diffusion range is limited,making it difficult to establish an effective displacement system.The variation in geostress exhibits diversity,with the change in horizontal minimum principal stress being greater than that in horizontal maximum principal stress,and the variation around the injection wells being more significant than that around the production wells.The deflection of geostress direction around injection wells is also large.The technology for EOR through multi-field reconstruction and combination of displacement and imbibition employs water injection wells converted to production and large-scale fracturing techniques to restructure the artificial fracture network system.Through a full lifecycle energy replenishment method of pre-fracturing energy supplementation,energy increase during fracturing,well soaking for energy storage,and combination of displacement and imbibition,it effectively addresses the issue of easy channeling of the injection medium and difficult energy replenishment after large-scale fracturing.By intensifying the imbibition effect through the coordination of multiple wells,it reconstructs the combined system of displacement and imbibition under a complex fracture network,transitioning from avoiding fractures to utilizing them,thereby improving microscopic sweep and oil displacement efficiencies.Field application in Block Yuan 284 of the Huaqing Oilfield in the Ordos Basin has demonstrated that this technology increases the recovery factor by 12 percentage points,enabling large scale and efficient development of tight oil.
基金Supported by the National Science and Technology Major Project of China(2016ZX05046-001).
文摘Based on the typical dissection of various onshore tight oil fields in China,the tight oil migration and accumulation mechanism and enrichment-controlling factors in continental lake basins are analyzed through nuclear magnetic resonance(NMR)displacement physical simulation and Lattice Boltzmann numerical simulation by using the samples of source rock,reservoir rock and crude oil.In continental lake basins,the dynamic forces driving hydrocarbon generation and expulsion of high-quality source rocks are the foundational power that determines the charging efficiency and accumulation effect of tight oil,the oil migration resistance is a key element that influences the charging efficiency and accumulation effect of tight oil,and the coupling of charging force with pore-throat resistance in tight reservoir controls the tight oil accumulation and sweet spot enrichment.The degree of tight oil enrichment in continental lake basins is controlled by four factors:source rock,reservoir pore-throat size,anisotropy of reservoir structure,and fractures.The high-quality source rocks control the near-source distribution of tight oil,reservoir physical properties and pore-throat size are positively correlated with the degree of tight oil enrichment,the anisotropy of reservoir structure reveals that the parallel migration rate is the highest,and intralayer fractures can improve the migration and accumulation efficiency and the oil saturation.
文摘Countercurrent imbibition is an important mechanism for tight oil recovery,that is,water imbibes spontaneously from the fracture into the porous matrix while oil flows reversely into the fracture.Its significance over cocurrent imbibition and forced imbibition is highlighted when permeability reduces.We used the computed tomography(CT)scanning to measure the one-dimensional evolution of water saturation profile and countercurrent imbibition distance(CID)at different fluid pressures,initial water saturations,and permeability.Surprisingly,experiments show that CID evolution for tight reservoir cores dramatically deviates from the classical diffusive rule(i.e.,evolutes proportional to square root of time,t^(0.5)).At early stage,CID extends faster than t^(0.5)(super-diffusive);while at late stage,CID extends much slower than t^(0.5)(sub-diffusive).After tens of hours,the CID change becomes too slow to be practically efficient for tight oil recovery.This research demonstrates that this deviation from classic theory is a result of(1)a much longer characteristic capillary length than effective invasion depth,which eliminates full development of a classical displacement front;and(2)non-zero flow at low water saturation,which was always neglected for conventional reservoir and is amplified in sub-mili-Darcy rocks.To well depict the details of the imbibition front in this situation,we introduce non-zero wetting phase fluidity at low saturation into classical countercurrent imbibition model and conduct numerical simulations,which successfully rationalizes the non-diffusive behavior and fits experimental data.Our data and theory imply an optimum soaking time in tight oil recovery by countercurrent imbibition,beyond which increasing exposed fracture surface area becomes a more efficient enhanced oil recovery(EOR)strategy than soaking for longer time.
基金the following funds:the National Key R&D Program of China(Project No.2017YFD0502200)the National Natural Science Foundation of China(Project No.31960721)the National Natural Science Foundation of China(Project No.31873034)。
文摘Background:Bacillus cereus is an important pathogen that causes human food poisoning,specifically diarrhea and vomiting.B.cereus can also induce mastitis in dairy cows and has a strong survival ability in milk,as it cannot be inactivated by high-temperature short-time pasteurization.Therefore,B.cereus can enter the market through pasteurized milk and other dairy products,imposing enormous hidden dangers on food safety and human health.Results:In this study,B.cereus 2101(BC)was isolated from milk samples of cows with mastitis.BC grew rapidly with strong hemolysis,making it difficult to prevent mastitis and ensure food security.MAC-T cells were treated with BC and/or Lactobacillus rhamnosus GR-1(LGR-1).Pretreatment with LGR-1 protected the integrity of tight junctions and the expression of zonula occludens-1(ZO-1)and occludin destroyed by BC.Furthermore,LGR-1 pretreatment reduced the expression of NOD-like receptor family member pyrin domain-containing protein 3(NLRP3),caspase recruitment and activation domain(ASC),Caspase-1 p20,gasdermin D(GSDMD)p30,inflammatory factors(interleukin(IL)-1βand IL-18),and cell death induced by BC.Moreover,LGR-1 pretreatment reduced NLRP3 inflammasome activity and increased expressions of ZO-1 and occludin induced by lipopolysaccharides(LPS)+ATP stimulation.MAC-T cells were transfected with NLRP3 si RNA or MCC950 and/or treated with BC and/or LGR-1.NLRP3-si RNA transfection and MCC950 attenuated BC-induced NLRP3 inflammasome activity.Expression of inflammatory cytokines and cell death suggested that the inflammatory pathway might play an important role in the induction of the NLRP3 inflammasome by BC and the protection of LGR-1.Conclusions:These results suggest that LGR-1 might be a probiotic alternative to antibiotics and could be administered to prevent mastitis in dairy cows,thus ensuring food security.
基金The work is supported by the Strategic Cooperation Technology Projects of CNPC and CUPB(ZLZX2020)by the Fundamental Research Funds for the Central Universities of China.
文摘Due to the poor physical properties of tight reservoirs,CO_(2) huff-n-puff(HNP)is considered a potential enhanced oil recovery(EOR)method after primary depletion.Optimization plays a critical role in the effective implementation of CO_(2) huff-n-puff.But the optimization requires a good understanding of the EOR mechanisms.In this work,the spatial distribution of oil saturation under different experimental conditions was analyzed by the NMR method to further discuss the HNP mechanisms.According to the variation of 1D frequency signal amplitude,we divided the core into the hardly movable area and movable area,the region with the obvious signal decline was defined as the movable area,and the hardly movable area was the region with limited signal decline.Based on that the recovery characteristics of different scenarios were evaluated.Firstly,the necessity of the soaking stage was studied,where three scenarios with different soaking times were carried out.Secondly,the injection pressure was adjusted to investigate the effect of the pressure gradient.The T_(2) spectra show that soaking has significantly improved the production of crude oil in small pores,and higher oil recovery in a single cycle is observed,but it is lower when the elapsed time(total operation time)is the same.31.03% of oil can be recovered after 3 cycles HNP,which increases to 33.8% and 37.06% for the 4 cycles and 6 cycles cases.As the pressure gradient increases,more oil is removed out of the matrix,and the oil in the deep part of the reservoir can be effectively recovered.During the CO_(2) huff-n-puff process,the oil distributions are similar to the solution gas drive,the residual oil is distributed at the close end of the core and the range that the oil can be efficiently recovered is limited.
基金This study is financially supported by CNPC Innovation Foundation(2020D-5007-0214)Major Strategic Project of CNPC(ZLZX2020-01-04)Beijing Municipal Excellent Talent Training Funds Youth Advanced Individual Project(2018000020124G163)。
文摘Tight conglomerate reservoirs are featured with extremely low permeability,strong heterogeneity and poor water injectivity.CO_(2) huff-n-puff has been considered a promising candidate to enhance oil recovery in tight reservoirs,owing to its advantages in reducing oil viscosity,improving mobility ratio,quickly replenishing formation pressure,and potentially achieving a miscible state.However,reliable inhouse laboratory evaluation of CO_(2) huff-n-puff in natural conglomerate cores is challenging due to the inherent high formation pressure.In this study,we put forward an equivalent method based on the similarity of the miscibility index and Grashof number to acquire a lab-controllable pressure that features the flow characteristics of CO_(2) injection in a tight conglomerate reservoir.The impacts of depletion degree,pore volume injection of CO_(2) and soaking time on ultimate oil recovery in tight cores from the Mahu conglomerate reservoir were successfully tested at an equivalent pressure.Our results showed that oil recovery decreased with increased depletion degree while exhibiting a non-monotonic tendency(first increased and then decreased)with increased CO_(2) injection volume and soaking time.The lower oil recoveries under excess CO_(2) injection and soaking time were attributed to limited CO_(2) dissolution and asphaltene precipitation.This work guides secure and reliable laboratory design of CO_(2) huff-n-puff in tight reservoirs with high formation pressure.
文摘The chronic complications of diabetes mellitus constitute a major public health problem.For example,diabetic eye diseases are the most important cause of blindness,and diabetic nephropathy is the most frequent cause of chronic kidney disease worldwide.The cellular and molecular mechanisms of these chronic complications are still poorly understood,preventing the development of effective treatment strategies.Tight junctions(TJs)are epithelial intercellular junctions located at the most apical region of cell-cell contacts,and their main function is to restrict the passage of molecules through the paracellular space.The TJs consist of over 40 proteins,and the most important are occludin,claudins and the zonula occludens.Accumulating evidence suggests that TJ disruption in different organs,such as the brain,nerves,retina and kidneys,plays a fundamental pathophysiological role in the development of chronic complications.Increased permeability of the blood-brain barrier and the blood-retinal barrier has been demonstrated in diabetic neuropathy,brain injury and diabetic retinopathy.The consequences of TJ disruption on kidney function or progression of kidney disease are currently unknown.In the present review,we highlighted the molecular events that lead to barrier dysfunction in diabetes.Further investigation of the mechanisms underlying TJ disruption is expected to provide new insights into therapeutic approaches to ameliorate the chronic complications of diabetes mellitus.
基金Sinopec Science and Technology Major Project (P22081)China National Science and Technology Major Project (2016ZX05002-004)
文摘Unconventional gas in the Sichuan Basin mainly includes shale gas and tight gas.The development of shale gas is mainly concentrated in the Ordovician Wufeng Formation-Silurian Longmaxi Formation,but has not made any significant breakthrough in the Cambrian Qiongzhusi Formation marine shale regardless of exploration efforts for years.The commercial development of tight sandstone gas is mainly concentrated in the Jurassic Shaximiao Formation,but has not been realized in the widespread and thick Triassic Xujiahe Formation.Depending on the geological characteristics of the Qiongzhusi Formation and Xujiahe Formation,the feedback of old wells was analyzed.Then,combining with the accumulation mechanisms of con-ventional gas and shale gas,as well as the oil/gas shows during drilling,changes in production and pressure during develop-ment,and other characteristics,it was proposed to change the exploration and development strategy from source and reservoir exploration to carrier beds exploration.With the combination of effective source rock,effective carrier beds and effective sand-stone or shale as the exploration target,a model of unconventional gas accumulation and enrichment in carrier beds was built.Under the guidance of this study,two significant results have been achieved in practice.First,great breakthrough was made in exploration of the silty shale with low organic matter abundance in the Qiongzhusi Formation,which breaks the traditional approach to prospect shale gas only in organic-rich black shales and realizes a breakthrough in new areas,new layers and new types of shale gas and a transformation of exploration and development of shale gas from single-layer system,Longmaxi For-mation,to multi-layer system in the Sichuan Basin.Second,exploration breakthrough and high-efficient development were re-alized for difficult-to-produce tight sandstone gas reserves in the Xujiahe Formation,which helps address the challenges of low production and unstable production of fracture zones in the Xujiahe Formation,promote the transformation of tight sandstone gas from reserves without production to effective production,and enhance the exploration and development potential of tight sandstonegas.
基金Supported by the Sinopec Science and Technology Project(P21040-1).
文摘In the second member of the Upper Triassic Xujiahe Formation(T_(3)x_(2))in the Xinchang area,western Sichuan Basin,only a low percent of reserves has been recovered,and the geological model of gas reservoir sweet spot remains unclear.Based on a large number of core,field outcrop,test and logging-seismic data,the T_(3)x_(2) gas reservoir in the Xinchang area is examined.The concept of fault-fold-fracture body(FFFB)is proposed,and its types are recognized.The main factors controlling fracture development are identified,and the geological models of FFFB are established.FFFB refers to faults,folds and associated fractures reservoirs.According to the characteristics and genesis,FFFBs can be divided into three types:fault-fracture body,fold-fracture body,and fault-fold body.In the hanging wall of the fault,the closer to the fault,the more developed the effective fractures;the greater the fold amplitude and the closer to the fold hinge plane,the more developed the effective fractures.Two types of geological models of FFFB are established:fault-fold fracture,and matrix storage and permeability.The former can be divided into two subtypes:network fracture,and single structural fracture,and the later can be divided into three subtypes:bedding fracture,low permeability pore,and extremely low permeability pore.The process for evaluating favorable FFFB zones was formed to define favorable development targets and support the well deployment for purpose of high production.The study results provide a reference for the exploration and development of deep tight sandstone oil and gas reservoirs in China.
基金This work was supported by the National Natural Science Foundation of China(No.51874320).
文摘Spontaneous imbibition is an important phenomenon in tight reservoirs.The existence of a large number of fractures and micro-nano pores is the key factor affecting the spontaneous imbibition of tight reservoirs.In this study,based on high-pressure mercury injection and nuclear magnetic resonance experiments,the pore distribution of tight sandstone is described.The influence of fractures,core porosity and permeability,and surfactants on the spontaneous imbibition of tight sandstone are studied by physical fracturing,interfacial tension test,wettability test and imbibition experiments.The results show that:the pore radius of tight sandstone is concentrated in 0.01-1 mm.Fractures can effectively reduce the oil drop adsorption on the core surface,enhancing the imbibition recovery of the tight sandstone with an increase of about 10%.As the number of fractures increases,the number of oil droplets adsorbed on the core surface decrease and the imbibition rate increases.The imbibition recovery increases with the increase in pore connectivity,while the imbibition rate increases with the increases in core porosity and permeability.The surfactant can improve the core water wettability and reduce the oilwater interfacial tension,reducing the adsorption of oil droplets on the core surface,and improving the core imbibition recovery with an increase of about 15%.In a word,the existence of fractures and surfactants can enhance the pore connectivity of the reservoir,reduce the adsorption of oil droplets on the core surface,and improve the imbibition rate and recovery rate of the tight oil reservoir.
基金The authors wish to thank the Ministry of Science and Technology of the People's Republic of China(2016ZX05066).
文摘Fuzzy-ball working fluids(FBWFs)have been successfully applied in different development phases of tight reservoirs.Field reports revealed that FBWFs satisfactorily met all the operational and reservoir damage control requirements during their application.However,the damage-control mechanisms and degree of formation damage caused by fuzzy-ball fluids have not been investigated in lab-scale studies so far.In this study,the degree of fuzzy-ball-induced damage in single-and double-layer reservoirs was evaluated through core flooding experiments that were based on permeability and flow rate indexes.Additionally,its damage mechanisms were observed via scanning electron microscope and energy-dispersive spectroscopy tests.The results show that:(1)For single-layer reservoirs,the FBWF induced weak damage on coals and medium-to-weak damage on sandstones,and the difference of the damage in permeability or flow rate index on coals and sandstones is below 1%.Moreover,the minimum permeability recovery rate was above 66%.(2)For double-layer commingled reservoirs,the flow rate index revealed weak damage and the overall damage in double-layer was lower than the single-layer reservoirs.(3)There is no significant alteration in the microscopic structure of fuzzy-ball saturated cores with no evidence of fines migration.The dissolution of lead and sulfur occurred in coal samples,while tellurium in sandstone,aluminum,and magnesium in carbonate.However,the precipitation of aluminum,magnesium,and sodium occurred in sandstone but no precipitates found in coal and carbonate.The temporal plugging and dispersion characteristics of the FBWFs enable the generation of reservoir protection layers that will minimize formation damage due to solid and fluid invasion.
基金supported by the National Natural Science Foundation of China(51674049,52074044,and 51874053)the Scientific Research Foundation of Hunan Provincial Education Department,China(22B0854)。
文摘In this study,X-ray diffraction,N_(2)adsorption(N_(2)A),and mercury intrusion(MI)experiments were used to investigate the influence of acid treatment on pore structure and fractal characterization of tight sandstones.The results showed that acid treatment generated a certain number of ink-bottle pores in fine sandstone,aggravated the ink-bottle effect in the sandy mudstone,and transformed some smaller pores into larger ones.After the acid treatment,both the pore volume in the range of 2–11 nm and 0.271–8μm for the fine sandstone and the entire pore size range for the sandy mudstone significantly increased.The dissolution of sandstone cement causes the fine sandstone particles to fall off and fill the pores;the porosity increased at first but then decreased with acid treatment time.The fractal dimension obtained using the Frenkel-Halsey-Hill model was positively correlated with acid treatment time.However,the total fractal dimensions obtained by MI tests showed different changes with acid treatment time in fine sandstone and sandy mudstone.These results provide good guiding significance for reservoir acidification stimulation.
基金We acknowledge the funding support from the National Science Foundation of China(Grant No.52278402)the Young Scientist Project of the National Key Research and Development Program of China(Grant No.2021YFC2900600)the Fundamental Research Funds for the Central Universities of China(Grant No.22120220117).
文摘During the operation of compressed air storage energy system,the rapid change of air pressure in a cavern will cause drastic changes in air density and permeability coefficient of sealing layer.To calculate and properly evaluate air tightness of polymer sealing caverns,the air-pressure-related air density and permeability must be considered.In this context,the high-pressure air penetration in the polymer sealing layer is studied in consideration of thermodynamic change of the cavern structure during the system operation.The air tightness model of compressed air storage energy caverns is then established.In the model,the permeability coefficient and air density of sealing layer vary with air pressure,and the effectiveness of the model is verified by field data in two test caverns.Finally,a compressed air storage energy cavern is taken as an example to understand the air tightness.The air leakage rate in the caverns is larger than that using air-pressure-independent permeability coefficient and air density,which is constant and small in the previous leakage rate calculation.Under the operating pressure of 4.5-10 MPa,the daily air leakage in the compressed air storage energy cavern of Yungang Mine with high polymer butyl rubber as the sealing material is 0.62%,which can meet the sealing requirements of compressed air storage energy caverns.The air tightness of the polymer sealing cavern is mainly affected by the cavern operating pressure,injected air temperature,cavern radius,and sealing layer thickness.The cavern air leakage rate will be decreased to reduce the cavern operating pressure the injection air temperature,or the cavern radius and sealing layer thickness will be increased.