Tiller angle, a very essential agronomic trait, is significant in rice breeding, especially in plant type breeding. A tiller anglo controlling 2 （tac2） mutant was obtained from a restorer line Jinhui 10 by ethyl met...Tiller angle, a very essential agronomic trait, is significant in rice breeding, especially in plant type breeding. A tiller anglo controlling 2 （tac2） mutant was obtained from a restorer line Jinhui 10 by ethyl methane sulphonate mutagenesis. The tac2 mutant displayed normal phenotype at the seedling stage and the tiller angle significantly increased at the tillering stage, A preliminary physiological research indicated that the mutant was sensitive to GA. Thus, it is speculated that TAC2 and TAC1 might control the tiller angle in the same way. Genetic analysis showed that the mutant trait was controlled by a major recessive gene and was located on chromosome 9 using SSR markers. The genetic distances between TAC2 and its nearest markers RM3320 and RM201 were 19.2 cM and 16,7 cM, respectively.展开更多
Tillering is an important agronomic trait which has a direct impact on plant type and grain yield. Strigolactones are a class of important phytohormones regulating rice tillering. ATMAX1 is an important gene involved ...Tillering is an important agronomic trait which has a direct impact on plant type and grain yield. Strigolactones are a class of important phytohormones regulating rice tillering. ATMAX1 is an important gene involved in strigolactone biosynthesis through encoding the protein P450 in Arabidopsis. Based on sequence BLASTp, we identified five homologous genes of ATMAX1 in rice, i.e., OsMAXla, OsMAXlb, OsMAXlc, OsMAXld and OsMAXle. Among them, OsMAXla and OsMAXle showed stable and high expression in rice tissues. In addition, we observed that OsMAXla and OsMAXle can rescue the branched phenotype and the influences caused by MAX1 mutation in Arabidopsis. Moreover, the expression of OsMAX1a and OsMAXle can respond to phosphate deficiency and different phytohormones, especially GR24, a strigolactone analogue. Therefore, it is concluded that OsMAX1a and OsMAX1e are involved in the biosynthesis of strigolactones and regulated rice tillering.展开更多
A spontaneous mutation, tentatively named d63, was derived from the twin-seedling progenies of rice crossed by diploid SARIII and Minghui 63. Compared with wild-type plants, the d63 mutant showed multiple abnormal phe...A spontaneous mutation, tentatively named d63, was derived from the twin-seedling progenies of rice crossed by diploid SARIII and Minghui 63. Compared with wild-type plants, the d63 mutant showed multiple abnormal phenotypes, such as dwarfism, more tillers, smaller flag leaf and reduced seed-setting rate and 1000-grain weight. In this study, two F2 populations were developed by crossing between d63 and Nipponbare, d63 and 93-11. Genetic analysis indicated that d63 was controlled by a single recessive gene, which was located on the short arm of chromosome 8, within the genetic distance of 0.40 cM from RM22195. Hence, D63 might be a new gene as there are no dwarf genes reported on the short arm of chromosome 8.展开更多
水稻分蘖角度是构建理想株型的关键因素,雄性不育株系是杂交水稻育种的重要种质资源。为进一步解析分蘖角度和花粉发育的调控机制,本研究通过农艺性状调查、育性检测、扫描和透射电镜观察等方法,比较野生型S40和经甲基磺酸乙酯(EMS)诱...水稻分蘖角度是构建理想株型的关键因素,雄性不育株系是杂交水稻育种的重要种质资源。为进一步解析分蘖角度和花粉发育的调控机制,本研究通过农艺性状调查、育性检测、扫描和透射电镜观察等方法,比较野生型S40和经甲基磺酸乙酯(EMS)诱变得到的散生雄性不育突变体lpms1(lazy and partially male sterile 1)的表型差异。结果表明,与野生型相比,lpms1突变体的分蘖角度增大,育性降低,并伴随株高、粒长、穗粒数、结实率和千粒重等重要性状不同程度降低。扫描和透射电镜观察到lpms1的成熟花粉粒表面皱缩、空瘪,内容物不充实,花粉壁异常。实时荧光定量PCR(q RT-PCR)结果显示,lpms1突变会引起花粉发育相关基因的表达发生差异。遗传分析结果表明,lpms1是单基因隐性突变。利用株型紧凑材料与lpms1杂交构建F_(2)群体,结合分离群体分组分析测序方法(BSA-seq)和图位克隆方法进行基因定位。最终,LPMS1基因定位于第5号染色体插入缺失(Indel)标记A3与A4之间298 kb的范围内,该区间有38个开放阅读框(ORFs),但无分蘖角度和花粉发育相关基因报道。LPMS1是新的分蘖角度和花粉发育基因,该基因突变会同时引起水稻分蘖角度增加、育性降低。本研究结果为水稻分蘖角度和花粉发育的调控机制研究以及水稻株型和育性改良种质的创制提供了新的遗传资源。展开更多
基金supported by the Doctoral Fund of Ministry of Education of China (Grant No. 20070635005)Ministry of Major Science & Technology of Chongqing, China (Grant No. CSTC2007AA1019)
文摘Tiller angle, a very essential agronomic trait, is significant in rice breeding, especially in plant type breeding. A tiller anglo controlling 2 （tac2） mutant was obtained from a restorer line Jinhui 10 by ethyl methane sulphonate mutagenesis. The tac2 mutant displayed normal phenotype at the seedling stage and the tiller angle significantly increased at the tillering stage, A preliminary physiological research indicated that the mutant was sensitive to GA. Thus, it is speculated that TAC2 and TAC1 might control the tiller angle in the same way. Genetic analysis showed that the mutant trait was controlled by a major recessive gene and was located on chromosome 9 using SSR markers. The genetic distances between TAC2 and its nearest markers RM3320 and RM201 were 19.2 cM and 16,7 cM, respectively.
文摘Tillering is an important agronomic trait which has a direct impact on plant type and grain yield. Strigolactones are a class of important phytohormones regulating rice tillering. ATMAX1 is an important gene involved in strigolactone biosynthesis through encoding the protein P450 in Arabidopsis. Based on sequence BLASTp, we identified five homologous genes of ATMAX1 in rice, i.e., OsMAXla, OsMAXlb, OsMAXlc, OsMAXld and OsMAXle. Among them, OsMAXla and OsMAXle showed stable and high expression in rice tissues. In addition, we observed that OsMAXla and OsMAXle can rescue the branched phenotype and the influences caused by MAX1 mutation in Arabidopsis. Moreover, the expression of OsMAX1a and OsMAXle can respond to phosphate deficiency and different phytohormones, especially GR24, a strigolactone analogue. Therefore, it is concluded that OsMAX1a and OsMAX1e are involved in the biosynthesis of strigolactones and regulated rice tillering.
文摘A spontaneous mutation, tentatively named d63, was derived from the twin-seedling progenies of rice crossed by diploid SARIII and Minghui 63. Compared with wild-type plants, the d63 mutant showed multiple abnormal phenotypes, such as dwarfism, more tillers, smaller flag leaf and reduced seed-setting rate and 1000-grain weight. In this study, two F2 populations were developed by crossing between d63 and Nipponbare, d63 and 93-11. Genetic analysis indicated that d63 was controlled by a single recessive gene, which was located on the short arm of chromosome 8, within the genetic distance of 0.40 cM from RM22195. Hence, D63 might be a new gene as there are no dwarf genes reported on the short arm of chromosome 8.
文摘水稻分蘖角度是构建理想株型的关键因素,雄性不育株系是杂交水稻育种的重要种质资源。为进一步解析分蘖角度和花粉发育的调控机制,本研究通过农艺性状调查、育性检测、扫描和透射电镜观察等方法,比较野生型S40和经甲基磺酸乙酯(EMS)诱变得到的散生雄性不育突变体lpms1(lazy and partially male sterile 1)的表型差异。结果表明,与野生型相比,lpms1突变体的分蘖角度增大,育性降低,并伴随株高、粒长、穗粒数、结实率和千粒重等重要性状不同程度降低。扫描和透射电镜观察到lpms1的成熟花粉粒表面皱缩、空瘪,内容物不充实,花粉壁异常。实时荧光定量PCR(q RT-PCR)结果显示,lpms1突变会引起花粉发育相关基因的表达发生差异。遗传分析结果表明,lpms1是单基因隐性突变。利用株型紧凑材料与lpms1杂交构建F_(2)群体,结合分离群体分组分析测序方法(BSA-seq)和图位克隆方法进行基因定位。最终,LPMS1基因定位于第5号染色体插入缺失(Indel)标记A3与A4之间298 kb的范围内,该区间有38个开放阅读框(ORFs),但无分蘖角度和花粉发育相关基因报道。LPMS1是新的分蘖角度和花粉发育基因,该基因突变会同时引起水稻分蘖角度增加、育性降低。本研究结果为水稻分蘖角度和花粉发育的调控机制研究以及水稻株型和育性改良种质的创制提供了新的遗传资源。
