Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissue...Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissues,but the applications are thus far mainly limited by the accuracy and precision of the TCSPC-FLIM technique.Here we present a comprehensive in-vestigation on the perforance of two data analysis methods,the first moment(M_(1))method and the conventional least squares(Fitting)method,in quantifying fuorescence lifetime.We found that the Mp method is more superior than the Fitting method when the lifetime is short(70-400ps)or the signal intensity is weak(<10^(3) photons).展开更多
基金supported by National Basic Research Program of China(Grant No.2011CB910401)the Science Fund for Creative Research Group of China(Grant No.61121004)+1 种基金the National Natural Sci-ence Foundation of China(Grant Nos.30970691 and 61275059)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of China,and the Program for New Century Excellent Talents in University of China(Grant No.NCET-10-0407).
文摘Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissues,but the applications are thus far mainly limited by the accuracy and precision of the TCSPC-FLIM technique.Here we present a comprehensive in-vestigation on the perforance of two data analysis methods,the first moment(M_(1))method and the conventional least squares(Fitting)method,in quantifying fuorescence lifetime.We found that the Mp method is more superior than the Fitting method when the lifetime is short(70-400ps)or the signal intensity is weak(<10^(3) photons).
