Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitorin...Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.展开更多
基于光时域反射技术(Optical Time Domain Reflectometry, OTDR)的光纤分布式传感器可以实现对整个传感光纤空间可分辨的分布式测量,相比点式传感器具有极大的技术及应用成本优势。而传统的基于模拟探测的OTDR光纤分布式传感器在空间分...基于光时域反射技术(Optical Time Domain Reflectometry, OTDR)的光纤分布式传感器可以实现对整个传感光纤空间可分辨的分布式测量,相比点式传感器具有极大的技术及应用成本优势。而传统的基于模拟探测的OTDR光纤分布式传感器在空间分辨率及动态范围上存在性能瓶颈。基于单光子探测的光子计数OTDR光纤分布式传感系统通过数字化的探测和记录方式,可以突破传统OTDR系统的性能极限。本文对光子计数OTDR系统技术及发展进行了综述,旨在通过本文的综述,明确基于单光子探测的光子计数OTDR系统的优势及限制,以及该技术的未来发展趋势,促进基于OTDR技术的光纤分布式传感器的进一步发展。展开更多
Fluorescence lifetime measurement in the time domain requires excitation from a well separated single bunch using synchrotron light sources. In the colliding mode of the Beijing Electron Positron Collider Ⅱ （BEPCⅡ...Fluorescence lifetime measurement in the time domain requires excitation from a well separated single bunch using synchrotron light sources. In the colliding mode of the Beijing Electron Positron Collider Ⅱ （BEPCⅡ）, a hybrid filling pattern was realized such that a single bunch was placed in the middle of a large gap between two multi-bunch groups. Detection of fluorescence lifetime, based on the excitation of the light pulse from this designated single-bunch, was established at Beamline 4B8 of the Beijing Synchrotron Radiation Facility （BSRF）. The timing signal of the BEPCII was utilized as a trigger to gate this fluorescence event. L-Tryptophan amino acid, a known lifetime standard, was selected to assess the lifetime measurement performance. The measured lifetime was consistent in both colliding and single-bunch mode with the time resolution down to 450 ps. Moreover, both the bunch purity and the fine structure of the hybrid filling pattern were characterized.展开更多
基金support from the National Key R&D Program of China(2017YFA0700500)National Natural Science Foundation of China(61775144/61525503/61620106016/61835009/81727804)+2 种基金(Key)Project of Department of Education of Guangdong Province(2015KGJHZ002/2016KCXTD007)Guangdong Natural Science Foundation(2014A030312008,2017A030310132,2018A030313362)Shenzhen Basic Research Project(JCYJ20170818144012025/JCYJ20170818141701667/JCYJ20170412105003520/JCYJ20150930104948169).
文摘Fluorescence lifetime imaging microscopy(FLIM)is increasingly used in biomedicine,material science,chemistry,and other related research fields,because of its advantages of high specificity and sensitivity in monitoring cellular microenvironments,studying interaction between proteins,metabolic state,screening drugs and analyzing their efficacy,characterizing novel materials,and diagnosing early cancers.Understandably,there is a large interest in obtaining FLIM data within an acquisition time as short as possible.Consequently,there is currently a technology that advances towards faster and faster FLIM recording.However,the maximum speed of a recording technique is only part of the problerm.The acquisition time of a FLIM image is a complex function of many factors.These include the photon rate that can be obtained from the sample,the amount of information a technique extracts from the decay functions,the fficiency at which it determines fluorescence decay parameters from the recorded photons,the demands for the accuracy of these parameters,the number of pixels,and the lateral and axial resolutions that are obtained in biological materials.Starting from a discussion of the parameters which determine the acquisition time,this review will describe existing and emerging FLIM techniques and data analysis algo-rithms,and analyze their performance and recording speed in biological and biomedical applications.
文摘基于光时域反射技术(Optical Time Domain Reflectometry, OTDR)的光纤分布式传感器可以实现对整个传感光纤空间可分辨的分布式测量,相比点式传感器具有极大的技术及应用成本优势。而传统的基于模拟探测的OTDR光纤分布式传感器在空间分辨率及动态范围上存在性能瓶颈。基于单光子探测的光子计数OTDR光纤分布式传感系统通过数字化的探测和记录方式,可以突破传统OTDR系统的性能极限。本文对光子计数OTDR系统技术及发展进行了综述,旨在通过本文的综述,明确基于单光子探测的光子计数OTDR系统的优势及限制,以及该技术的未来发展趋势,促进基于OTDR技术的光纤分布式传感器的进一步发展。
基金Supported by National Natural Science Foundation of China (10635060,20871116)Innovation Fund of Institute of HighEnergy Physics (IHEP)
文摘Fluorescence lifetime measurement in the time domain requires excitation from a well separated single bunch using synchrotron light sources. In the colliding mode of the Beijing Electron Positron Collider Ⅱ （BEPCⅡ）, a hybrid filling pattern was realized such that a single bunch was placed in the middle of a large gap between two multi-bunch groups. Detection of fluorescence lifetime, based on the excitation of the light pulse from this designated single-bunch, was established at Beamline 4B8 of the Beijing Synchrotron Radiation Facility （BSRF）. The timing signal of the BEPCII was utilized as a trigger to gate this fluorescence event. L-Tryptophan amino acid, a known lifetime standard, was selected to assess the lifetime measurement performance. The measured lifetime was consistent in both colliding and single-bunch mode with the time resolution down to 450 ps. Moreover, both the bunch purity and the fine structure of the hybrid filling pattern were characterized.
