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混合地理加权空间自回归模型的Profile GMM估计
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作者 马笑笑 王韶郡 魏传华 《应用数学》 北大核心 2023年第1期237-247,共11页
为了更好地同时考虑空间自相关性和空间异质性,本文研究一类空间自回归混合地理加权回归模型.基于Profile方法和广义矩(GMM)方法,构造了模型中未知空间自回归参数,常数回归系数和系数函数的两类Profile GMM估计.数值模拟结果表明所提出... 为了更好地同时考虑空间自相关性和空间异质性,本文研究一类空间自回归混合地理加权回归模型.基于Profile方法和广义矩(GMM)方法,构造了模型中未知空间自回归参数,常数回归系数和系数函数的两类Profile GMM估计.数值模拟结果表明所提出的估计在有限样本中表现良好. 展开更多
关键词 空间自回归 地理加权回归 广义矩估计 profile方法
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Energy properties and spatial plume profile of ionic liquid ion sources based on an array of porous metal strips 被引量:3
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作者 刘欣宇 康小明 +1 位作者 邓涵文 孙逸鸣 《Plasma Science and Technology》 SCIE EI CAS CSCD 2021年第12期128-139,共12页
An ionic liquid ion source(ILIS)is a kind of high brightness ion source capable of providing high-speed positive or negative ion beams.This paper presents a miniaturized ILIS based on an array of porous metal strips.T... An ionic liquid ion source(ILIS)is a kind of high brightness ion source capable of providing high-speed positive or negative ion beams.This paper presents a miniaturized ILIS based on an array of porous metal strips.The porous emitter array,integrated with seven 10 mm long strips,is fabricated using wire electrical discharge machining(WEDM)combined with electrochemical etching.The assembled ILIS is 30 mm×30 mm×17.5 mm in size and weighs less than 25 g.A series of experiments,including anⅠ-Ⅴcharacteristic test,a retarding potential analyzer(RPA)test,and a spatial plume distribution test,have been conducted in vacuo to characterize the performance of the ILIS.Results show that the emitted current is up to about 800μA and ion transparency is as high as 94%.Besides,RPA curves reveal that the total fragmentation rate of the emitted particles accounts for 48.8%in positive mode and 59.8%in negative mode.Further,with the increase in applied acceleration voltage,the voltage loss rises while the energy efficiency decreases.It is also found that the plume perpendicular to the strips has a higher divergence than the one parallel to the strips.A numerical simulation by COMSOL reveals that the electric field distribution between the two electrodes results in such a spatial plume profile. 展开更多
关键词 ELECTROSPRAY ionic liquid ion source retarding potential analyzer energy distribution spatial plume profile
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Optimization of Injection Parameters for Profile Control and Flooding in an Oilfield during High Water Cut Period
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作者 Meinan Wang Hui Cai +2 位作者 Xiaoqi Chen Junting Zhang Yue Xie 《Journal of Geoscience and Environment Protection》 2023年第11期73-81,共9页
In order to improve the effect of water control and oil stabilization during high water cut period, a mathematical model of five point method well group was established with the high water cut well group of an Oilfiel... In order to improve the effect of water control and oil stabilization during high water cut period, a mathematical model of five point method well group was established with the high water cut well group of an Oilfield as the target area, the variation law of water cut and recovery factor of different injection parameters was analyzed, and the optimization research of injection parameters of polymer enhanced foam flooding was carried out. The results show that the higher the injection rate, the lower the water content curve, and the higher the oil recovery rate. As the foam defoamed when encountering oil, when the injection time was earlier than 80% of water cut, the later the injection time was, the better the oil displacement effect would be. When the injection time was later than 80% of water cut, the later the injection time was, the worse the oil displacement effect would be. The larger the injection volume, the lower the water content curve and the higher the recovery rate. After the injection volume exceeded 0.2 PV, the amplitude of changes in water content and recovery rate slowed down. The optimal injection parameters of profile control agent for high water content well group in Oilfield A were: injection rate of 15 m<sup>3</sup>/d, injection timing of 80% water content, and injection volume of 0.2 PV. 展开更多
关键词 High Water Cut Period profile Control Injection Rate Injection Timing Injection Volume
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Expression profiles of penaeidin from Fenneropenaeus chinensis in response to WSSV and vibrio infection by real-time PCR 被引量:3
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作者 DONGBo LIUFengsong +2 位作者 XIANGJianhai LIFuhua GAOHongwei 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2005年第2期131-140,共10页
Penaeidin from Chinese shrimp (Fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study... Penaeidin from Chinese shrimp (Fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study through time, the mRNA expression profile of penaeidin in the muscle and haemocyte tissue of Chinese shrimp infected with vibrio (Vibrio anguillarum) and WSSV (white spot syndrome virus). Research results showed that the same pathogens infection experiments produced similar gene expression profile in different tissues while different expression profiles appeared in the same tissues infected by different exterior pathogens. In vibrio infection experiments, a 'U' like expression profile resulted. Expression levels of penaeidin increased and surpassed the non-stimulated level, indicating that penaeidin from Chinese shrimp has noticeable antimicrobial activities. In WSSV infection experiments, the expression profile appeared as an inverse 'U' with the expression of penaeidin gradually decreasing to below baseline level after 24 h. The expression of antimicrobial peptides gene in mRNA level in response to virus infection in shrimp showed that international mechanisms of virus to haemocytes and microbial to haemocytes are completely different. Decline of penaeidins expression levels may be due to haemocytes being destroyed by WSSV or that the virus can inhibit the expression of penaeidins by yet undiscovered modes. The expression profiles of penaeidin in response to exterior pathogen and the difference of expression profiles between vibrio and WSSV infection provided some clues to further understanding the complex innate immune mechanism in shrimp. 展开更多
关键词 penaeidin Fenneropenaeus chinensis expression profile WSSV Vibrio anguillarum real-time PCR
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Clustering gully profiles for investigating the spatial variation in landform formation on the Chinese Loess Plateau
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作者 CHENG Yao LI Jing-wei +1 位作者 XIONG Li-yang TANG Guo-an 《Journal of Mountain Science》 SCIE CSCD 2021年第10期2742-2760,共19页
The gully is the most dynamic and changeable landform unit on the Loess Plateau,and the characteristics of gully landforms are key indicators of gully evolution.Different gully profiles are connected and combined thro... The gully is the most dynamic and changeable landform unit on the Loess Plateau,and the characteristics of gully landforms are key indicators of gully evolution.Different gully profiles are connected and combined through runoff nodes.Thus,it is necessary to cluster gully profiles into a gully profile combination(GPC)to reveal the spatial variation in gully landforms throughout the Loess Plateau.First,the gradient and gully evolution index(GEI)of two sample areas in Changwu and Suide in Shaanxi Province,China are calculated and analysed based on GPC.Then,the gradient and GEI are calculated by using 90-m-resolution digital elevation model(DEM)data for the severe soil erosion area with the basin as the research unit.On this basis,the spatial variation in the development degree is analysed with Getis-Ord Gi*.The results show that the degree of gully undercutting decreases from southeast to northwest under the influence of rainfall.Due to the soil properties,the loess in the northwest is more prone to collapse,resulting in the decrease of GEI from northwest to southeast.The development degree of gullies is closely related to rivers.The strong erosive capacity of rivers leads to greater differences in gullies within the basin.At the same time,the skewness and kurtosis of the gully index in the basin are correlated;when the distribution of the gully index in the basin is less normal,the distribution of the gully index is more concentrated.These results reveal the spatial variation characteristics of the Loess Plateau based on GPC. 展开更多
关键词 Gully profile combination spatial variation Digital elevation model Loess landform Terrain analysis
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Analysis of Temperature Profiles and Cycle Time in a Large-Scale Medical Waste Incinerator
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作者 Veilla E. Matee Samwel V. Manyele 《Engineering(科研)》 2015年第10期717-732,共16页
Temperature profiles and cycle times in a large-scale medical waste incinerator installed in a referral hospital were used to assess the performance and functionality of incinerator. The study was conducted using data... Temperature profiles and cycle times in a large-scale medical waste incinerator installed in a referral hospital were used to assess the performance and functionality of incinerator. The study was conducted using data collected from 8 cycles per days for 67 days. For proper combustion and destruction of toxic components in the primary chamber and destruction of pollutants and toxic components in the flue gas, it is desired to reach the maximum temperature in the chambers faster and maintain this maximum temperature for an extended time interval. The primary and secondary temperatures T1 and T2, respectively, were recorded at an interval of one minute for different cycles. Different amounts of wastes with varying proportions of sharps and other wastes were loaded into the incinerator and temperature profiles recorded. The analysis shows that the incinerator works at primary temperature less than the required recommended by manufacturer while the secondary chamber operates between 600 and above 950℃, although higher temperatures up to 1020℃ were observed. The average load preparation time was observed to be 14.6 minutes, while the chamber preheating time before daily initial loading was 25.45 minutes. Both temperature profiles were observed to have similar shapes for all combustion cycles studied, except when incinerator malfunctioning occurred. The average cycle time was established to be 32.7 minutes and 28.97 minutes based on time to drop to 550℃ after the maximum temperature and loading time intervals, respectively, although longer cycle times were observed. Temperature drop in both combustion chambers as a result of waste charging was observed in the interval of 5 minutes. The chamber heating rate was observed to decrease exponentially with time during both preheating and incineration operation. 展开更多
关键词 Medical WASTE Management INFECTIOUS WASTE PATHOLOGICAL WASTE Sharps WASTE INCINERATION Temperature profile Cycle time HEATING Rate
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A Calculation Method of Profile Error Influenced by Tool Error for Two-Axis Coordinated CNC Machining of a Spatial Barrel-Cam
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作者 YIN Ming-Fu, LU Chuan-Yi, CHEN Jin-Kui Xi’an University of Technology, Xi’an 710048, P. R. China Shandong Institute of Technology, Zibo 255012, P. R. China 《International Journal of Plant Engineering and Management》 2001年第2期92-97,共6页
Taking the CNC machining for the spatial barrel-cam with rectilinear translating and a conical roller follower as an example, the calculation method and the law of the profile error influenced by the tool error is given.
关键词 spatial barrel-cam CNC machining profile error calculation method
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Show Time通用电气Profile Wine Chiller酒柜
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《新潮电子》 2005年第15期106-106,共1页
在遥远优雅的欧罗巴大陆,葡萄酒的生产不是作为一种工业,而是种植、天气、土壤、人的劳动与传统工艺,宗教相融合的种神圣仪式,随之而来,饮用和储藏也极其讲究。所以,就不难明白那些对美酒情有独钟的人为什么会如何“金屋藏娇”而... 在遥远优雅的欧罗巴大陆,葡萄酒的生产不是作为一种工业,而是种植、天气、土壤、人的劳动与传统工艺,宗教相融合的种神圣仪式,随之而来,饮用和储藏也极其讲究。所以,就不难明白那些对美酒情有独钟的人为什么会如何“金屋藏娇”而费尽心思了。如果你恰好有此雅好,通用电气的这台独立式中心酒窖或许可以帮你打点妥当。它能够存放最多29瓶你最喜爱的酒,易用的电子触摸控制允许你选择红和白葡萄酒的理想温度,使其长久保持浓郁的香醇。 展开更多
关键词 通用电气 profile SHOW time WINE 传统工艺 白葡萄酒 触摸控制 独立式 美酒 酒窖
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Methods to Improve the Long Distance Time-Varying Channel Transmission Performance of Expendable Profiler
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作者 ZHENG Yu ZHANG Xiao-yang +2 位作者 WANG Xiao-rui GUO Xing-xin LI Hong-zhi 《China Ocean Engineering》 SCIE EI CSCD 2019年第6期753-761,共9页
To improve the transmission performance of XCTD channel, this paper proposes a method to measure directly and fit the channel transmission characteristics by using frequency sweeping method. Sinusoidal signals with a ... To improve the transmission performance of XCTD channel, this paper proposes a method to measure directly and fit the channel transmission characteristics by using frequency sweeping method. Sinusoidal signals with a frequency range of 100 Hz to 10 k Hz and an interval of 100 Hz are used to measure transmission characteristics of channels with lengths of 300 m, 800 m, 1300 m, and 1800 m. The correctness of the fitted channel characteristics by transmitting square wave, composite waves of different frequencies, and ASK modulation are verified. The results show that when the frequency of the signal is below 1500 Hz, the channel has very little effect on the signal. The signal compensated for amplitude and phase at the receiver is not as good as the uncompensated signal.Alternatively, when the signal frequency is above 1500 Hz, the channel distorts the signal. The quality of signal compensated for amplitude and phase at receiver is better than that of the uncompensated signal. Thus, we can select the appropriate frequency for XCTD system and the appropriate way to process the received signals. Signals below1500 Hz can be directly used at the receiving end. Signals above 1500 Hz are used after amplitude and phase compensation at the receiving end. 展开更多
关键词 expandable conductivity temperature depth(XCTD)profiler time-varying channel modeling frequency sweeping method amplitude and phase compensation transmission performance
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A Dedicated UML Profile for Real Time Geomantic Modelisation and Code Generation
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作者 Ben Youssef Malek Lbath Ahmed Belhadj Ziad 《通讯和计算机(中英文版)》 2010年第10期25-32,共8页
关键词 实时应用 FEMLAB UML 代码生成 数据库应用程序 风水 统一建模语言 概念模型
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基于Matrix Profile的可扩展时间序列的算法研究
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作者 袁鸿燕 崔明星 《信息与电脑》 2020年第22期72-77,共6页
信息化时代的到来,正改变着人们的生活和学习方式.从时间序列数据库中提取频繁模式是一项重要的数据挖掘任务,这些模式也称为模体.发现时间序列中的模体是数据挖掘领域的新课题,在各个领域都拥有良好的应用前景,为许多预测开辟了新途径... 信息化时代的到来,正改变着人们的生活和学习方式.从时间序列数据库中提取频繁模式是一项重要的数据挖掘任务,这些模式也称为模体.发现时间序列中的模体是数据挖掘领域的新课题,在各个领域都拥有良好的应用前景,为许多预测开辟了新途径.虽然模体数据挖掘技术应用广泛,但在水文领域的应用研究还有待深入.本文以发现基于相似度的时间序列定长模体为基础目标,研究基于Matrix Profile的可扩展时间序列的算法,即STAMP算法,发现利用卷积来计算点积很明显优于直接计算,并对STAMP算法和BF算法进行了比较. 展开更多
关键词 时间序列 模体挖掘 Matrix profile
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Effect of varying spatial orientations on build time requirements for FDM process: A case study 被引量:1
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作者 Sandeep Rathee Manu Srivastava +1 位作者 Sachin Maheshwari Arshad Noor Siddiquee 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2017年第2期92-100,共9页
In this research, effect of varying spatial orientations on the build time requirements for fused deposition modelling process is studied. Constructive solid geometry cylindrical primitive is taken as work piece and m... In this research, effect of varying spatial orientations on the build time requirements for fused deposition modelling process is studied. Constructive solid geometry cylindrical primitive is taken as work piece and modeling is accomplished for it. Response surface methodology is used to design the experiments and obtain statistical models for build time requirements corresponding to different orientations of the given primitive in modeller build volume. Contour width, air gap, slice height, raster width, raster angle and angle of orientation are treated as process parameters. Percentage contribution of individual process parameter is found to change for build time corresponding to different spatial orientations. Also, the average of build time requirement changes with spatial orientation. This paper attempts to clearly discuss and describe the observations with an aim to develop a clear understanding of effect of spatial variations on the build time for Fused Deposition Modelling process. This work is an integral part of process layout optimization and these results can effectively aid designers specially while tackling nesting issues. 展开更多
关键词 FUSED deposition modeling spatial orientation PROCESS parameters Response Surface Methodology BUILD time
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Researches on the PAM/Cr^3+ Gel for Deep Profile Control 被引量:1
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作者 ChangXuejun LiuXuejun +1 位作者 WangQunyi GaoShusheng 《Petroleum Science》 SCIE CAS CSCD 2004年第1期65-69,共5页
The profile control hard-gel is composed of polyarylamide (5-6g/L), whose molecular weight is 4,000,000 - 7,000,000 and hydrolysis degree is 17.6%, and cross-linking oxidation-reduction agent (Na2Cr2O7 + NH4Cl), with ... The profile control hard-gel is composed of polyarylamide (5-6g/L), whose molecular weight is 4,000,000 - 7,000,000 and hydrolysis degree is 17.6%, and cross-linking oxidation-reduction agent (Na2Cr2O7 + NH4Cl), with an delayed organic acid crosslinker which contains lactic acid/propionic acid/ethanoic acid and ethylene glycol. After research of the influence factors, such as pH, temperature, salinity and the dosage of delayed crosslinker, the optimum condition(pH 5.2, temperature 55℃, salinity < 7g/L) was found. Gelation time (12-144h) can be controlled by adjusting the dosage of the delayed crosslinker. Deep profile control experiments are carried out on heterogeneous models, which contains three serial high permeable and low permeable cores arranged in a parallel. After water flooding (total recovery, 24.3%), the first, second and third high-permeable cores each are sealed in turn by the profile control agent, and the total displacement recovery increases to 46.8%, 62.2% and 69.1% respectively. So, the greater the sealed depth, the larger the enhancing recovery will be. Finally, the oil displacement mechanisms of deep profile control are discussed. 展开更多
关键词 Deep profile control hard-gel delayed cross-linker gelation time heterogeneous model sealed depth
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A Novel Method for the Reconstruction of Road Profiles from Measured Vehicle Responses Based on the Kalman Filter Method 被引量:2
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作者 Jianghui Zhu Xiaotong Chang +2 位作者 Xueli Zhang Yutai Su Xu Long 《Computer Modeling in Engineering & Sciences》 SCIE EI 2022年第3期1719-1735,共17页
The estimation of the disturbance input acting on a vehicle from its given responses is an inverse problem.To overcome some of the issues related to ill-posed inverse problems,this work proposes a method of reconstruc... The estimation of the disturbance input acting on a vehicle from its given responses is an inverse problem.To overcome some of the issues related to ill-posed inverse problems,this work proposes a method of reconstructing the road roughness based on the Kalman filter method.A half-car model that considers both the vehicle and equipment is established,and the joint input-state estimation method is used to identify the road profile.The capabilities of this methodology in the presence of noise are numerically demonstrated.Moreover,to reduce the influence of the driving speed on the estimation results,a method of choosing the calculation frequency is proposed.A road vibration test is conducted to benchmark the proposed method. 展开更多
关键词 Road profile reconstruction inverse problems precise integration Kalman filter spatial resolution
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Design and Analysis of Spatial Modulation Based Orthogonal Time Frequency Space System 被引量:3
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作者 Yingchao Yang Zhiquan Bai +4 位作者 Ke Pang Piming Ma Haixia Zhang Xinghai Yang Dongfeng Yuan 《China Communications》 SCIE CSCD 2021年第8期209-223,共15页
In this paper,we design a spatial modulation based orthogonal time frequency space(SMOTFS)system to achieve improved transmission reliability and meet the high transmission rate and highspeed demands of future mobile ... In this paper,we design a spatial modulation based orthogonal time frequency space(SMOTFS)system to achieve improved transmission reliability and meet the high transmission rate and highspeed demands of future mobile communications,which fully utilizes the characteristics of spatial modulation(SM)and orthogonal time frequency space(OTFS)transmission.The detailed system design and signal processing of the SM-OTFS system have been presented.The closed-form expressions of the average symbol error rate(ASER)and average bit error rate(ABER)of the SM-OTFS system have been derived over the delay-Doppler channel with the help of the union bounding technique and moment-generating function(MGF).Meanwhile,the system complexity has been evaluated.Numerical results verify the correctness of the theoretical ASER and ABER analysis of the SM-OTFS system in the high signal-to-noise ratio(SNR)regions and also show that the SM-OTFS system outperforms the traditional SM based orthogonal frequency division multiplexing(SM-OFDM)system with limited complexity increase under mobile conditions,especially in high mobility scenarios. 展开更多
关键词 orthogonal time frequency space(OTFS) spatial modulation based OTFS(SM-OTFS) delay-Doppler domain average symbol error rate(ASER) average bit error rate(ABER)
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Study of Frequency Effects on Hardness Profile of Spline Shaft Heat-Treated by Induction 被引量:1
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作者 Habib Hammi Abderazzak El Ouafi Noureddine Barka 《Journal of Materials Science and Chemical Engineering》 2016年第3期1-9,共9页
This paper is devoted to the study of frequency effects on hardness profile of AISI 4340 spline shaft heat-treated by induction through an extensive 3D finite element method simulation and structured experimental inve... This paper is devoted to the study of frequency effects on hardness profile of AISI 4340 spline shaft heat-treated by induction through an extensive 3D finite element method simulation and structured experimental investigation. Based on coupled electromagnetic and thermal fields analysis, the 3D model is used to estimate the temperature distribution and the hardness profile. The proposed study examines the hardening process parameters, such as frequency, induced current density and heating time, known to have an influence on hardened surface and builds the simulation model step by step. The established model can provide not only an accurate prediction of temperature distribution and hardness profile but also a comprehensive analysis of machine parameters effects, especially the frequency. The numerical results achieved by this model are good and present a great agreement to the experimental data. 展开更多
关键词 Induction Heating Splines Shaft Hardness profile Current Density Heating time FREQUENCY
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Route Temporal⁃Spatial Information Based Residual Neural Networks for Bus Arrival Time Prediction 被引量:1
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作者 Chao Yang Xiaolei Ru Bin Hu 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2020年第4期31-39,共9页
Bus arrival time prediction contributes to the quality improvement of public transport services.Passengers can arrange departure time effectively if they know the accurate bus arrival time in advance.We proposed a mac... Bus arrival time prediction contributes to the quality improvement of public transport services.Passengers can arrange departure time effectively if they know the accurate bus arrival time in advance.We proposed a machine⁃learning approach,RTSI⁃ResNet,to forecast the bus arrival time at target stations.The residual neural network framework was employed to model the bus route temporal⁃spatial information.It was found that the bus travel time on a segment between two stations not only had correlation with the preceding buses,but also had common change trends with nearby downstream/upstream segments.Two features about bus travel time and headway were extracted from bus route including target section in both forward and reverse directions to constitute the route temporal⁃spatial information,which reflects the road traffic conditions comprehensively.Experiments on the bus trajectory data of route No.10 in Shenzhen public transport system demonstrated that the proposed RTSI⁃ResNet outperformed other well⁃known methods(e.g.,RNN/LSTM,SVM).Specifically,the advantage was more significant when the distance between bus and the target station was farther. 展开更多
关键词 bus arrival time prediction route temporal⁃spatial information residual neural network recurrent neural network bus trajectory data
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A STUDY ON EQUILIBRIUM COASTAL PROFILES OF THE CLOSE TIDAL FLAT—A Case Study of Fengxian Tidal Flat 被引量:1
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作者 LI Heng peng, YANG Gui shan(Nanjing Institute of Geography and Limnology, the Chinese Academy of Sciences, Nanjing 210008, P.R. China) 《Chinese Geographical Science》 SCIE CSCD 2002年第1期55-60,共6页
The article analyses the temporal spatial changes of profiles by EOF (Empirical Orthogonal Function) analysis and DTM analysis of GIS. These profiles, which are not affected by engineering, are chosen from the coast w... The article analyses the temporal spatial changes of profiles by EOF (Empirical Orthogonal Function) analysis and DTM analysis of GIS. These profiles, which are not affected by engineering, are chosen from the coast with successive field monitoring data from 1990 to 1999. Temporal and spatial EOF indicates the obvious stability of coast profile parameters in Fengxian tidal flat. In spatial scale, high tidal flats and deep water terraces are in a balance state while upper clino with steep slopes are sensitive and the stability is easy to be destroyed. In temporal scale, the erosion and deposition in this area are kept in balance in a whole. There are almost no change below 8- 9.5m. At the same time, it is the lower limit of tidal affection and the erosion and deposition process from it to high tidal flat keep in balance for many years. So the closure depth is appointed to from 8m to 9.5 m (Wusong datum mark). 展开更多
关键词 经验正交函数分析 空间分析 GIS 平衡剖面 海岸剖面 空间分析 DTM
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Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling 被引量:3
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作者 Kang Kang Xiao Peng +1 位作者 Jun Luo Deming Gou 《Journal of Animal Science and Biotechnology》 SCIE 2012年第2期51-59,共9页
MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the posttranscriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Der... MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the posttranscriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation. Deregulation of miRNAs i n serum or plasma has been associated with many diseases including cancers and cardiovascular diseases, suggesting the possible use of miRNAs as diagnostic biomarkers. However, the detection of the small amount of miRNAs found in serum or plasma requires a method with high sensitivity and accuracy. Therefore, the current study describes polymerase chain reaction (PCR)-based methods for measuring circulating miRNAs. Briefly, the procedure involves four major steps: (1) sample collection and preparation; (2) global miRNAs profiling using quantitative real-time PCR (qRT-PCR); (3) data normalization and analysis; and (4) selection and validation of miRNA biomarkers. In conclusion, qRT-PCR is a promising method for profiling of circulating miRNAs as biomarkers. 展开更多
关键词 BIOMARKER circulating microRNAs profilING quantitative real-time PCR
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Spatial Pattern and Time Series Dynamics of Spondylis buprestoides Adults
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作者 Shuyong ZHOU Huihua CHEN Ningyu XIANG 《Plant Diseases and Pests》 CAS 2012年第2期38-41,共4页
Spondylis buprestoides adults in Pians masoniana forests in Xianju Dabei Dixi Forestry Center were continuously investigated during 2006 and 2011. According to the survey data, multiple spatial pattern indicators of a... Spondylis buprestoides adults in Pians masoniana forests in Xianju Dabei Dixi Forestry Center were continuously investigated during 2006 and 2011. According to the survey data, multiple spatial pattern indicators of adult population were calculated, and the relationship between various indicators and density was analyzed. The K values of negative binomial distribution less affected by density were selected to describe the spatial pattern and time series dynamics of S. buprestoides adults. The results indicated that S. buprestoides adults showed aggregated distribution in the forest, but the aggregation degree varied with the season. There were 2 obvious diffusion peaks during May and June as well as September and October each year. The aggregation trend within a generation was aggregation-diffusion-aggregation. 展开更多
关键词 Spondylis buprestoides POPULATION spatial pattern time series dynamics
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