为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、...为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、香茅醛、柠檬醛)进行了定性和定量分析。该方法简便、高效,在25 min内山苍子精油中各成分分离良好。各成分在一定范围内均线性关系良好(R2>0.999),精密度、稳定性及重复性均符合要求,平均加标回收率为93.40%~107.35%。结果表明:该方法符合方法学验证要求,可用于山苍子精油的多成分含量测定,并为其质量评价提供依据。展开更多
The method was proposed for detecting the pesticide residues such as organophosphorus and carbamates by GC/MS/SIM.The method is rapid and highly sensitive for 15 pesticide residues.
The application of MS in newborn screening is one of the most important branches of MSs current clinical application. In this paper we first reported a new method of newborn screening for Phenylketonuria (PKU) by GC...The application of MS in newborn screening is one of the most important branches of MSs current clinical application. In this paper we first reported a new method of newborn screening for Phenylketonuria (PKU) by GC-CI-MS-SIM, which was relatively cheap, fast, sensitive and accurate. In this method Phenylalanine (Phe) and Tyrosine (Tyr) were extracted from dry blood spots with 0.1% mol/L HCl-methanol. The protein in the extraction was gotten rid of by centrifugor. Phe and Tyr were reacted with 3 0 mol/L HCl-butanol, then the products Phe and Tyr butyl esters were reacted with trifluoroacetic anhydride. The final derivations from Phe and Tyr, alanine, 3-phenyl-N-(trifluoroacetyl)-, butyl este and L-tyrosine, N(trifluoroacetyl)-, butyl ester, trifl were analyzed by GC-CI-MS-SIM. The contents of the two amino acids in blood samples were determined by calculating the areas of their final derivations with exterior standard. Finally, the molar ratios of Phe to Tyr in blood sample were calculated as the basis of PKU diagnosis. Because chemical ionization and selective ion measurement were used in this method, the limits of detection for Phe and Tyr were low and the sensitivity was excellent. About 30 blood samples were analyzed by this method, and the results prove that this method could successfully discriminate between normal infants and PKU positive ones. The price of instrument GC-MS is cheaper than that of MS-MS. Therefore it was capable for large-scale screening in developing countries.展开更多
文摘为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、香茅醛、柠檬醛)进行了定性和定量分析。该方法简便、高效,在25 min内山苍子精油中各成分分离良好。各成分在一定范围内均线性关系良好(R2>0.999),精密度、稳定性及重复性均符合要求,平均加标回收率为93.40%~107.35%。结果表明:该方法符合方法学验证要求,可用于山苍子精油的多成分含量测定,并为其质量评价提供依据。
文摘The method was proposed for detecting the pesticide residues such as organophosphorus and carbamates by GC/MS/SIM.The method is rapid and highly sensitive for 15 pesticide residues.
文摘The application of MS in newborn screening is one of the most important branches of MSs current clinical application. In this paper we first reported a new method of newborn screening for Phenylketonuria (PKU) by GC-CI-MS-SIM, which was relatively cheap, fast, sensitive and accurate. In this method Phenylalanine (Phe) and Tyrosine (Tyr) were extracted from dry blood spots with 0.1% mol/L HCl-methanol. The protein in the extraction was gotten rid of by centrifugor. Phe and Tyr were reacted with 3 0 mol/L HCl-butanol, then the products Phe and Tyr butyl esters were reacted with trifluoroacetic anhydride. The final derivations from Phe and Tyr, alanine, 3-phenyl-N-(trifluoroacetyl)-, butyl este and L-tyrosine, N(trifluoroacetyl)-, butyl ester, trifl were analyzed by GC-CI-MS-SIM. The contents of the two amino acids in blood samples were determined by calculating the areas of their final derivations with exterior standard. Finally, the molar ratios of Phe to Tyr in blood sample were calculated as the basis of PKU diagnosis. Because chemical ionization and selective ion measurement were used in this method, the limits of detection for Phe and Tyr were low and the sensitivity was excellent. About 30 blood samples were analyzed by this method, and the results prove that this method could successfully discriminate between normal infants and PKU positive ones. The price of instrument GC-MS is cheaper than that of MS-MS. Therefore it was capable for large-scale screening in developing countries.
