An rooting experiment of tissue culture plantlets was carried out with sterile plantlets obtained from the stem segments of a good clone of Camellia oleifera as materials. The results showed that basic medium and illu...An rooting experiment of tissue culture plantlets was carried out with sterile plantlets obtained from the stem segments of a good clone of Camellia oleifera as materials. The results showed that basic medium and illumination condition are factors crucial to rooting of C. oleifera. With 1/4MS as basic medium, the treat- ment with the addition of 0.5 mg/L NAA and soaking in 2 000 mg/L KIBA, containing 30 mg/L sucrose and subjected to dark culture of 20 d was the optimal treat- ment, achieving a rooting rate of 86. 7%.展开更多
DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study...DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study, methylation-sensitive amplified polymorphisms （MSAP） were used to investigate DNA methylation profiles of cassava polyploidy following cold treatment to identify candidate genes involved in response to cold stress. The result showed that the genome-wide DNA methylation polymorphisms accounted for 34.02%-42.56% in SC8 and its autotetraploid exposed to 5 ~C for 2, 8, 24 and 48 h, respectively. The methylation levels of SC8 at 2 h-cold stress were the highest during 48h under cold treatments. With the time extension within 48 h under cold stress, the methylation levels gradually decreased to the same level as the control but DNA methylation levels of cassava autotetraploid were stable within 48 h. For future analysis of the methylation extent, the cold stress induced more DNA methylation than demethylation in SC8, where DNA methylation was consistent with demethylation in its autotetraploid. The expression analysis demonstrated increase in the transcription of one methylated gene and decrease in the transcription of two demethylated genes. The results revealed that gene methylations in specific sites would be a rapidly epigenetic response to cold stress, further elucidating the methylation functions in its autotetraploid.展开更多
基金Supported by Camellia oleifera Industry Development Fund of Hunan Province
文摘An rooting experiment of tissue culture plantlets was carried out with sterile plantlets obtained from the stem segments of a good clone of Camellia oleifera as materials. The results showed that basic medium and illumination condition are factors crucial to rooting of C. oleifera. With 1/4MS as basic medium, the treat- ment with the addition of 0.5 mg/L NAA and soaking in 2 000 mg/L KIBA, containing 30 mg/L sucrose and subjected to dark culture of 20 d was the optimal treat- ment, achieving a rooting rate of 86. 7%.
文摘DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study, methylation-sensitive amplified polymorphisms （MSAP） were used to investigate DNA methylation profiles of cassava polyploidy following cold treatment to identify candidate genes involved in response to cold stress. The result showed that the genome-wide DNA methylation polymorphisms accounted for 34.02%-42.56% in SC8 and its autotetraploid exposed to 5 ~C for 2, 8, 24 and 48 h, respectively. The methylation levels of SC8 at 2 h-cold stress were the highest during 48h under cold treatments. With the time extension within 48 h under cold stress, the methylation levels gradually decreased to the same level as the control but DNA methylation levels of cassava autotetraploid were stable within 48 h. For future analysis of the methylation extent, the cold stress induced more DNA methylation than demethylation in SC8, where DNA methylation was consistent with demethylation in its autotetraploid. The expression analysis demonstrated increase in the transcription of one methylated gene and decrease in the transcription of two demethylated genes. The results revealed that gene methylations in specific sites would be a rapidly epigenetic response to cold stress, further elucidating the methylation functions in its autotetraploid.
