Objective To investigate the effects of sodium arsenite（NaAsO2）on the expression of microRNA-191（miR-191）and tissue inhibitor of metalloproteinase 3（TIMP-3）in human normal hepatic cells（L-02 cells）.Methods L-0...Objective To investigate the effects of sodium arsenite（NaAsO2）on the expression of microRNA-191（miR-191）and tissue inhibitor of metalloproteinase 3（TIMP-3）in human normal hepatic cells（L-02 cells）.Methods L-02 cells were exposed to different doses of Na2As O2[0（control group）,5,25,50 and 75μmol/L]展开更多
Renal cell carcinoma(RCC)has a poor prognosis due to limited diagnosis and treatment.Thus,it is necessary to find novel prognostic biomarkers and therapeutic targets.The aberrant expression of microRNAs plays an impor...Renal cell carcinoma(RCC)has a poor prognosis due to limited diagnosis and treatment.Thus,it is necessary to find novel prognostic biomarkers and therapeutic targets.The aberrant expression of microRNAs plays an important role in RCC oncogenesis.Tissue inhibitors of metalloproteinase 3(TIMP3)acts as a downstream target of miR-181b.The aim of this study was to understand the role and molecular mechanism of miR-181b in RCC oncogenesis.The results showed that miR-181b expression was significantly higher in RCC tumour tissues,especially in those with significant invasion or metastasis.miR-181b overexpression promoted proliferation and migration of the RCC cell line 786-O,while miR-181b knockdown had the opposite effect.In addition,miR-181b was inversely correlated with TIMP3 expression in RCC tumour tissues.miR-181b overexpression reduced TIMP3 expression in RCC cell line 786-O or OS-RC-2,while miR-181b knockdown had the inverse effect.Mechanistically,a luciferase reporter assay confirmed the binding sites of miR-181b on the 3’-UTR of TIMP3,confirming the targeting effect of miR-181b on TIMP3.Overall,miR-181b promotes the development and progression of RCC by targeting TIMP3 expression,indicating the potential use of miR-181b in the diagnosis and treatment of RCC.展开更多
文摘Objective To investigate the effects of sodium arsenite（NaAsO2）on the expression of microRNA-191（miR-191）and tissue inhibitor of metalloproteinase 3（TIMP-3）in human normal hepatic cells（L-02 cells）.Methods L-02 cells were exposed to different doses of Na2As O2[0（control group）,5,25,50 and 75μmol/L]
基金This work was supported by grants Basic Scientific Research Projects of Fujian Provincial Public Welfare Scientific Research Institutes(2016R1029-2).
文摘Renal cell carcinoma(RCC)has a poor prognosis due to limited diagnosis and treatment.Thus,it is necessary to find novel prognostic biomarkers and therapeutic targets.The aberrant expression of microRNAs plays an important role in RCC oncogenesis.Tissue inhibitors of metalloproteinase 3(TIMP3)acts as a downstream target of miR-181b.The aim of this study was to understand the role and molecular mechanism of miR-181b in RCC oncogenesis.The results showed that miR-181b expression was significantly higher in RCC tumour tissues,especially in those with significant invasion or metastasis.miR-181b overexpression promoted proliferation and migration of the RCC cell line 786-O,while miR-181b knockdown had the opposite effect.In addition,miR-181b was inversely correlated with TIMP3 expression in RCC tumour tissues.miR-181b overexpression reduced TIMP3 expression in RCC cell line 786-O or OS-RC-2,while miR-181b knockdown had the inverse effect.Mechanistically,a luciferase reporter assay confirmed the binding sites of miR-181b on the 3’-UTR of TIMP3,confirming the targeting effect of miR-181b on TIMP3.Overall,miR-181b promotes the development and progression of RCC by targeting TIMP3 expression,indicating the potential use of miR-181b in the diagnosis and treatment of RCC.
