RNA editing exists extensively in the higher plant mitochondria, and is a required step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind ...RNA editing exists extensively in the higher plant mitochondria, and is a required step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind of phenomenon that is attributed to mitochondrial genome mutations. The research materials used are the gametophytic male sterility line (A), maintainer line (B) and F1 hybrid (F1) of HL-type CMS rice. cDNAs and DNAs of atp6 and coxII have been obtained from A, B and F1 by PCR and RT-PCR. Comparing sequences of cDNAs and DNAs, 18 and 15 editing sites were found respectively in the transcripts of atp6 and coxll. A, B and F1 shared the same editing sites. RNA editing improves hydrophobicity and conservation of the predicted protein as compared with other organisms.展开更多
基金This work was supported by the National Natural Science Foundation of China (Grant No. 3070568).
文摘RNA editing exists extensively in the higher plant mitochondria, and is a required step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind of phenomenon that is attributed to mitochondrial genome mutations. The research materials used are the gametophytic male sterility line (A), maintainer line (B) and F1 hybrid (F1) of HL-type CMS rice. cDNAs and DNAs of atp6 and coxII have been obtained from A, B and F1 by PCR and RT-PCR. Comparing sequences of cDNAs and DNAs, 18 and 15 editing sites were found respectively in the transcripts of atp6 and coxll. A, B and F1 shared the same editing sites. RNA editing improves hydrophobicity and conservation of the predicted protein as compared with other organisms.
基金Ministry of EducationCulture+13 种基金SportsScienceand Technology of Japan Grants-in-Aid for Scientific Research(编号:1739025119390235与20023008负责人:Shin K)Ministry of HealthLaborand Welfare of Japan Grant(编号:H18-Kokoro-017负责人:Shin K)日本肌萎缩侧索硬化症协会基金(编号:875 P.H.S.)上海浦江人才计划资助项目(编号:09PJ1409300负责人:潘卫东)上海市中医药事业发展3年行动计划(重大研究)项目(ZYSNXD-CC-ZDYJ028负责人:蔡定芳)
