Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-...Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression.In this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and evaluated.The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed tissues.Different combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt methods.The screened reference genes were verified by normalizing EjLGA1 in each subgroup.The obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage stress.This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat.To our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E.japonica.The use of the three most stable reference genes could increase the reliability of future quantification experiments.展开更多
Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. These viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV EN...Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. These viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV ENT infections can lead to benign or malignant tumors in which we could find both LR-HPV and HR-HPV genotypes. The objective of this study was to investigate the genotypes of HR-HPV and LR-HPV in archived tissue samples derived from both benign and malignant tumors of the ear, nose, and throat (ENT) in Ouagadougou, Burkina Faso. One hundred and twenty formalin-fixed, paraffin-embedded archived tissues of the ENT sphere from 26 benign tumors and 94 malignant tumors were included. The tissues were first deparaffinized with xylem. The extracted DNA was used to test for high-risk and low-risk HPV by Real-Time Multiplex PCR. HPV DNA was found in 57.7% (15/26) of benign tumors and 43.61% (41/94) of malignant tumors. The prevalence of HPV infection was 46.67% (56/120) in all tumors combined. The most common HPV genotypes found were HPV 11 (34.28%), HPV 6 (30%), HPV56 (14.28%) and HPV 33 (8.57%). There were 21.43% (12/56) cases of genotypes co-infections with 10 cases of double infection and 2 cases of triple infection. Both low-risk and high-risk HPV are found in ENT tumors with relatively high HPV prevalence.展开更多
Mercury is a ubiquitous contaminant known to accumulate in wildlife,particularly bird species at higher trophic levels.Knowledge of tissue-specific Hg distributions aids our understanding of Hg bioaccumulation in orga...Mercury is a ubiquitous contaminant known to accumulate in wildlife,particularly bird species at higher trophic levels.Knowledge of tissue-specific Hg distributions aids our understanding of Hg bioaccumulation in organisms.In this study,one adult and three juvenile Collared Scops Owls(Otus lettia)were studied to elucidate the bioaccumulation of Hg in body tissues.Six tissues and organs(feathers,nails,heart,liver,gizzard,and muscle),as well as gastric contents,were examined for total Hg(THg)and methylmercury(MeHg)contents,Hg isotopic compositions including mass-dependent fractionation(MDF;δ202Hg)and mass-independent fractionation(MIF;Δ199Hg andΔ201Hg),and C(δ13C)and N(δ15N)isotopic compositions.Tissue-specific THg and MeHg concentrations in the adult were in the ranges of 150–1360 ng/g and17–1060 ng/g,and lower in the juveniles at 91–419 ng/g and 67–350 ng/g,respectively.Theδ^(202)Hg values in the adult were strongly negative at-1.75‰±0.17‰compared with the juveniles at-0.99‰±0.25‰.The adult exhibited lower MIF values than the juveniles,at0.23‰±0.07‰forΔ^(199)Hg and 0.2‰±0.11‰forΔ^(201)Hg,comparedwith0.81‰±0.09‰and0.66‰±0.07‰,respectively.The lower adult MDF and MIF values suggest that the adult tended to accumulate negative Hg isotopes but the juvenile's positive Hg isotopes.Differences between adult and juvenile tissue Hg concentrations indicate that metabolic processes play an important role in Hg accumulation.展开更多
Tissue dielectric properties can vary upon the incident of an acoustic wave.The goal of this study is to quantify this change due to the acoustoelectric effect(AE),and to obtain the frequency-dependent dielectric prop...Tissue dielectric properties can vary upon the incident of an acoustic wave.The goal of this study is to quantify this change due to the acoustoelectric effect(AE),and to obtain the frequency-dependent dielectric properties of tissues exposed to low-intensity focused ultrasound(LIFU).The dielectric properties of the blood,brain,chest muscle,heart,kidney,leg muscle,liver,lung,pancreas,and spleen of rats were measured by an open-ended coaxial probe method.The acoustic intensity of LIFU focus was 2.97 MPa(67.6 W/cm^(2)),3.95 MPa(120 W/cm^(2)),and 5.17 MPa(204 W/cm^(2)),respectively,and the measurement frequency band was 0.1–7.08 GHz.The measurement results show that with the LIFU modulation,the conductivity and dielectric constant decreased in the high-frequency band,and on the contrary,they increased in the lowfrequency band,and the larger the acoustic intensity was,the more obvious the phenomenon was.This work contributes to a better understanding of the mechanisms by which ultrasound acts on the dielectric properties of biological tissues.It is expected that the findings from this study will provide a basis that the response of tissue to LIFU modulation can be monitored by noninvasive techniques such as microwave-induced thermoacoustic imaging(MTI)and microwave imaging,present a new idea for improving the endogenous contrast between different biological tissues in MTI and acoustoelectric imaging,and possibly lead to the development of a new imaging method based on the relaxation time of tissue after LIFU modulation.展开更多
Understanding of the heat transport within living biological tissues is crucial to effective heat treatments. The heat transport properties of living biological tissues with temperature-dependent properties are explor...Understanding of the heat transport within living biological tissues is crucial to effective heat treatments. The heat transport properties of living biological tissues with temperature-dependent properties are explored in this paper. Taking into account of variable physical properties, the governing equation of temperature is first derived in the context of the dualphase-lags model(DPL). An effective method, according to the Laplace transform and a linearization technique, is then employed to solve this nonlinear governing equation. The temperature distribution of a biological tissue exposed to a pulsed heat flux on its exterior boundary, which frequently happens in various heat treatments, is predicted and analyzed. The results state that a lower temperature can be predicted when temperature dependence is considered in the heating process.The contributions of key thermal parameters are different and dependent on the ratio of phase lag and the amplitude of the exterior pulsed heat flux.展开更多
The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery,which begins before the onset of the preterm labor process.T...The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery,which begins before the onset of the preterm labor process.Therefore,it is important to resolve the changes of collagen flbers during cervical remodeling for the prevention of preterm labor.Herein,we assessed the spatial organization of collagen flbers in a three-dimensional(3D)context within cervical tissues of mice on day 3,9,12,15 and 18 of gestation.We found that the 3D directional variance,a novel metric of alignment,was higher on day 9 than that on day 3 and then gradually decreased from day 9 to day 18.Compared with two-dimensional(2D)approach,a higher sensitivity was achieved from 3D analysis,highlighting the importance of truly 3D quantification.Moreover,the depthdependent variation of 3D directional variance was investigated.By combining multiple 3D directional variance-derived metrics,a high level of classification accuracy was acquired in distinguishing different periods of pregnancy.These results demonstrate that 3D directional variance is sensitive to remodeling of collagen fibers within cervical tissues,shedding new light on highly-sensitive,early detection of preterm birth(PTB).展开更多
Polarimetry encompasses a collection of optical techniques broadly used in a variety of fields.Nowadays,such techniques have provided their suitability in the biomedical field through the study of the polarimetric res...Polarimetry encompasses a collection of optical techniques broadly used in a variety of fields.Nowadays,such techniques have provided their suitability in the biomedical field through the study of the polarimetric response of biological samples(retardance,dichroism and depolarization)by measuring certain polarimetric observables.One of these features,depolarization,is mainly produced by scattering on samples,which is a predominant efiect in turbid media as biological tissues.In turn,retardance and dichroic efiects are produced by tissue anisotropies and can lead to depolarization too.Since depolarization is a predominant efiect in tissue samples,we focus on studying difierent depolarization metrics for biomedical applications.We report the suitability of a set of depolarizing observables,the indices of polarimetric purity(IPPs),for biological tissue inspection.We review some results where we demonstrate that IPPs lead to better performance than the depolarization index,which is a well-established and commonly used depolarization observable in the literature.We also provide how IPPs are able to significantly enhance contrast between difierent tissue structures and even to reveal structures hidden by using standard intensity images.Finally,we also explore the classificatory potential of IPPs and other depolarizing observables for the discrimination of difierent tissues obtained from ex vivo chicken samples(muscle,tendon,myotendinous junction and bone),reaching accurate models for tissue classification.展开更多
The cavitation dynamics and mechanical stress in viscoelastic tissues, as the primary mechanisms of some ultrasound therapies, are extremely complex due to the interactions of cavitation bubble with adjacent bubbles a...The cavitation dynamics and mechanical stress in viscoelastic tissues, as the primary mechanisms of some ultrasound therapies, are extremely complex due to the interactions of cavitation bubble with adjacent bubbles and surrounding tissues.Therefore, the cavitation dynamics and resultant mechanical stress of two-interacting bubbles in the viscoelastic tissues are numerically investigated, especially focusing on the effects of the adjacent bubble. The results demonstrate that the mechanical stress is highly dependent on the bubble dynamics. The compressive stress and tensile stress are generated at the stage of bubble expansion and collapse stage, respectively. Furthermore, within the initial parameters examined in this paper, the effects of the adjacent bubble will distinctly suppress the radial expansion of the small bubble and consequently lead its associated stresses to decrease. Owing to the superimposition of two stress fields, the mechanical stresses surrounding the small bubble in the direction of the neighboring bubble are smaller than those in other directions. For two interacting cavitation bubbles, the suppression effects of the nearby bubble on both the cavitation dynamics and the stresses surrounding the small bubble increase as the ultrasound amplitude and the initial radius of the large bubble increase, whereas they decrease with the inter-bubble distance increasing. Moreover, increasing the tissue viscoelasticity will reduce the suppression effects of the nearby bubble, except in instances where the compressive stress and tensile stress first increase and then decrease with the tissue elasticity and viscosity increasing respectively. This study can provide a further understanding of the mechanisms of cavitation-associated mechanical damage to the adjacent tissues or cells.展开更多
目的:从临床指标方面系统评价并比较自体软组织替代材料与结缔组织移植物联合冠向复位瓣技术治疗天然牙牙龈退缩的疗效。方法:计算机检索PubMed、EMBASE、Web of Science、维普、万方、知网等数据库中关于自体软组织替代材料或结缔组织...目的:从临床指标方面系统评价并比较自体软组织替代材料与结缔组织移植物联合冠向复位瓣技术治疗天然牙牙龈退缩的疗效。方法:计算机检索PubMed、EMBASE、Web of Science、维普、万方、知网等数据库中关于自体软组织替代材料或结缔组织移植物(CTG)治疗牙龈退缩的随机对照试验,检索时限从2013年1月至2023年12月。结果:最终纳入14个随机对照研究,1117个牙龈退缩位点。Meta分析显示,术后6个月时,CTG与异种胶原基质(XCM)比较能更好地降低牙龈退缩深度,与富血小板纤维蛋白(PRF)、脱细胞真皮基质(ADM)比较无明显区别;CTG与PRF、ADM、XCM比较角化龈宽度增量更明显;根面覆盖率指标差异无统计学意义;PRF、XCM可以明显降低患者术后的疼痛感。结论:研究表明,自体软组织替代材料与CTG相比,对于治疗牙龈退缩的效果并没有显著差异,但是前者可以降低患者术后疼痛感。展开更多
基金funded by the Natural Science Foundation of Fujian Province(2021J05240)Fujian Provincial Science and Technology Project(2021N5014,2022N5006)+1 种基金Research Project of Putian Science and Technology Bureau(2021ZP08,2021ZP09,2021ZP10,2021ZP11)Scientific Research Project of Putian University(2018064).
文摘Loquat(Eriobotrya japonica Lindl.)is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components.Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression.In this study,eight candidate reference genes were selected from our previously published RNA-seq data,and primers for each candidate reference gene were designed and evaluated.The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples,including 12 subgroups of developing or abiotic-stressed tissues.Different combinations of stable reference genes were screened according to a comprehensive rank,which was synthesized from the results of four algorithms,including the geNorm,NormFinder,BestKeeper andΔCt methods.The screened reference genes were verified by normalizing EjLGA1 in each subgroup.The obtained suitable combinations of reference genes for accurate normalization were GAPDH,EF1αand ACT for floral development;GAPDH,UBCE and ACT for fruit setting;EF1α,GAPDH and eIF2B for fruit ripening;ACT,EF1αand UBCE for leaves under heat stress;eIF2B,UBCE and EF1αfor leaves under freezing stress;EF1α,TUA and UBCE for leaves under salt stress;ACT,EF1αand eIF2B for immature pulp under freezing stress;ACT,UBCE and eIF2B for immature seeds under freezing stress;EF1α,eIF2B and UBCE for both immature pulp and seeds under freezing stress;UBCE,TUB and TUA for red-fleshed fruits under cold-storage stress;eIF2B,RPS3 and TUB for white-fleshed fruits under coldstorage stress;and eIF2B,UBCE and RPS3 for both red-and white-fleshed fruits under cold-storage stress.This study obtained different combinations of stable reference genes for accurate normalization in twelve subgroups of developing or abiotic-stressed tissues in loquat.To our knowledge,this is the first report to obtain stable reference genes for normalizing gene expression of abiotic-stressed tissues in E.japonica.The use of the three most stable reference genes could increase the reliability of future quantification experiments.
文摘Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. These viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV ENT infections can lead to benign or malignant tumors in which we could find both LR-HPV and HR-HPV genotypes. The objective of this study was to investigate the genotypes of HR-HPV and LR-HPV in archived tissue samples derived from both benign and malignant tumors of the ear, nose, and throat (ENT) in Ouagadougou, Burkina Faso. One hundred and twenty formalin-fixed, paraffin-embedded archived tissues of the ENT sphere from 26 benign tumors and 94 malignant tumors were included. The tissues were first deparaffinized with xylem. The extracted DNA was used to test for high-risk and low-risk HPV by Real-Time Multiplex PCR. HPV DNA was found in 57.7% (15/26) of benign tumors and 43.61% (41/94) of malignant tumors. The prevalence of HPV infection was 46.67% (56/120) in all tumors combined. The most common HPV genotypes found were HPV 11 (34.28%), HPV 6 (30%), HPV56 (14.28%) and HPV 33 (8.57%). There were 21.43% (12/56) cases of genotypes co-infections with 10 cases of double infection and 2 cases of triple infection. Both low-risk and high-risk HPV are found in ENT tumors with relatively high HPV prevalence.
基金supported by National Natural Science Foundation of China(NSFC No.42103080)。
文摘Mercury is a ubiquitous contaminant known to accumulate in wildlife,particularly bird species at higher trophic levels.Knowledge of tissue-specific Hg distributions aids our understanding of Hg bioaccumulation in organisms.In this study,one adult and three juvenile Collared Scops Owls(Otus lettia)were studied to elucidate the bioaccumulation of Hg in body tissues.Six tissues and organs(feathers,nails,heart,liver,gizzard,and muscle),as well as gastric contents,were examined for total Hg(THg)and methylmercury(MeHg)contents,Hg isotopic compositions including mass-dependent fractionation(MDF;δ202Hg)and mass-independent fractionation(MIF;Δ199Hg andΔ201Hg),and C(δ13C)and N(δ15N)isotopic compositions.Tissue-specific THg and MeHg concentrations in the adult were in the ranges of 150–1360 ng/g and17–1060 ng/g,and lower in the juveniles at 91–419 ng/g and 67–350 ng/g,respectively.Theδ^(202)Hg values in the adult were strongly negative at-1.75‰±0.17‰compared with the juveniles at-0.99‰±0.25‰.The adult exhibited lower MIF values than the juveniles,at0.23‰±0.07‰forΔ^(199)Hg and 0.2‰±0.11‰forΔ^(201)Hg,comparedwith0.81‰±0.09‰and0.66‰±0.07‰,respectively.The lower adult MDF and MIF values suggest that the adult tended to accumulate negative Hg isotopes but the juvenile's positive Hg isotopes.Differences between adult and juvenile tissue Hg concentrations indicate that metabolic processes play an important role in Hg accumulation.
基金the National Natural Science Foundation of China(Grant Nos.82071940 and 62001075)Chongqing Municipal Education Commission Youth Fund,China(Grant Nos.KJQN20200607 and KJQN20200610).
文摘Tissue dielectric properties can vary upon the incident of an acoustic wave.The goal of this study is to quantify this change due to the acoustoelectric effect(AE),and to obtain the frequency-dependent dielectric properties of tissues exposed to low-intensity focused ultrasound(LIFU).The dielectric properties of the blood,brain,chest muscle,heart,kidney,leg muscle,liver,lung,pancreas,and spleen of rats were measured by an open-ended coaxial probe method.The acoustic intensity of LIFU focus was 2.97 MPa(67.6 W/cm^(2)),3.95 MPa(120 W/cm^(2)),and 5.17 MPa(204 W/cm^(2)),respectively,and the measurement frequency band was 0.1–7.08 GHz.The measurement results show that with the LIFU modulation,the conductivity and dielectric constant decreased in the high-frequency band,and on the contrary,they increased in the lowfrequency band,and the larger the acoustic intensity was,the more obvious the phenomenon was.This work contributes to a better understanding of the mechanisms by which ultrasound acts on the dielectric properties of biological tissues.It is expected that the findings from this study will provide a basis that the response of tissue to LIFU modulation can be monitored by noninvasive techniques such as microwave-induced thermoacoustic imaging(MTI)and microwave imaging,present a new idea for improving the endogenous contrast between different biological tissues in MTI and acoustoelectric imaging,and possibly lead to the development of a new imaging method based on the relaxation time of tissue after LIFU modulation.
基金Project supported by the National Science Foundation of China (Grant Nos.51676086 and 51575247)。
文摘Understanding of the heat transport within living biological tissues is crucial to effective heat treatments. The heat transport properties of living biological tissues with temperature-dependent properties are explored in this paper. Taking into account of variable physical properties, the governing equation of temperature is first derived in the context of the dualphase-lags model(DPL). An effective method, according to the Laplace transform and a linearization technique, is then employed to solve this nonlinear governing equation. The temperature distribution of a biological tissue exposed to a pulsed heat flux on its exterior boundary, which frequently happens in various heat treatments, is predicted and analyzed. The results state that a lower temperature can be predicted when temperature dependence is considered in the heating process.The contributions of key thermal parameters are different and dependent on the ratio of phase lag and the amplitude of the exterior pulsed heat flux.
基金supported by the National Natural Science Foundation of China (61905214,62035011,11974310 and 31927801)National Key Research and Development Program of China (2019YFE0113700 and 2017YFA0700501)Natural Science Foundation of Zhejiang Province (LR20F050001).
文摘The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery,which begins before the onset of the preterm labor process.Therefore,it is important to resolve the changes of collagen flbers during cervical remodeling for the prevention of preterm labor.Herein,we assessed the spatial organization of collagen flbers in a three-dimensional(3D)context within cervical tissues of mice on day 3,9,12,15 and 18 of gestation.We found that the 3D directional variance,a novel metric of alignment,was higher on day 9 than that on day 3 and then gradually decreased from day 9 to day 18.Compared with two-dimensional(2D)approach,a higher sensitivity was achieved from 3D analysis,highlighting the importance of truly 3D quantification.Moreover,the depthdependent variation of 3D directional variance was investigated.By combining multiple 3D directional variance-derived metrics,a high level of classification accuracy was acquired in distinguishing different periods of pregnancy.These results demonstrate that 3D directional variance is sensitive to remodeling of collagen fibers within cervical tissues,shedding new light on highly-sensitive,early detection of preterm birth(PTB).
基金the financial support of Spanish MINECO(PID2021-126509OB-C21,and Fondos FEDER)Catalan Government(2017-SGR-001500).
文摘Polarimetry encompasses a collection of optical techniques broadly used in a variety of fields.Nowadays,such techniques have provided their suitability in the biomedical field through the study of the polarimetric response of biological samples(retardance,dichroism and depolarization)by measuring certain polarimetric observables.One of these features,depolarization,is mainly produced by scattering on samples,which is a predominant efiect in turbid media as biological tissues.In turn,retardance and dichroic efiects are produced by tissue anisotropies and can lead to depolarization too.Since depolarization is a predominant efiect in tissue samples,we focus on studying difierent depolarization metrics for biomedical applications.We report the suitability of a set of depolarizing observables,the indices of polarimetric purity(IPPs),for biological tissue inspection.We review some results where we demonstrate that IPPs lead to better performance than the depolarization index,which is a well-established and commonly used depolarization observable in the literature.We also provide how IPPs are able to significantly enhance contrast between difierent tissue structures and even to reveal structures hidden by using standard intensity images.Finally,we also explore the classificatory potential of IPPs and other depolarizing observables for the discrimination of difierent tissues obtained from ex vivo chicken samples(muscle,tendon,myotendinous junction and bone),reaching accurate models for tissue classification.
基金Project supported by the National Natural Science Foundation of China (Grant No.11904042)the Natural Science Foundation of Chongqing,China (Grant No.cstc2019jcyj-msxmX0534)the Science and Technology Research Program of Chongqing Municipal Education Commission,China (Grant No.KJQN202000617)。
文摘The cavitation dynamics and mechanical stress in viscoelastic tissues, as the primary mechanisms of some ultrasound therapies, are extremely complex due to the interactions of cavitation bubble with adjacent bubbles and surrounding tissues.Therefore, the cavitation dynamics and resultant mechanical stress of two-interacting bubbles in the viscoelastic tissues are numerically investigated, especially focusing on the effects of the adjacent bubble. The results demonstrate that the mechanical stress is highly dependent on the bubble dynamics. The compressive stress and tensile stress are generated at the stage of bubble expansion and collapse stage, respectively. Furthermore, within the initial parameters examined in this paper, the effects of the adjacent bubble will distinctly suppress the radial expansion of the small bubble and consequently lead its associated stresses to decrease. Owing to the superimposition of two stress fields, the mechanical stresses surrounding the small bubble in the direction of the neighboring bubble are smaller than those in other directions. For two interacting cavitation bubbles, the suppression effects of the nearby bubble on both the cavitation dynamics and the stresses surrounding the small bubble increase as the ultrasound amplitude and the initial radius of the large bubble increase, whereas they decrease with the inter-bubble distance increasing. Moreover, increasing the tissue viscoelasticity will reduce the suppression effects of the nearby bubble, except in instances where the compressive stress and tensile stress first increase and then decrease with the tissue elasticity and viscosity increasing respectively. This study can provide a further understanding of the mechanisms of cavitation-associated mechanical damage to the adjacent tissues or cells.
文摘目的:从临床指标方面系统评价并比较自体软组织替代材料与结缔组织移植物联合冠向复位瓣技术治疗天然牙牙龈退缩的疗效。方法:计算机检索PubMed、EMBASE、Web of Science、维普、万方、知网等数据库中关于自体软组织替代材料或结缔组织移植物(CTG)治疗牙龈退缩的随机对照试验,检索时限从2013年1月至2023年12月。结果:最终纳入14个随机对照研究,1117个牙龈退缩位点。Meta分析显示,术后6个月时,CTG与异种胶原基质(XCM)比较能更好地降低牙龈退缩深度,与富血小板纤维蛋白(PRF)、脱细胞真皮基质(ADM)比较无明显区别;CTG与PRF、ADM、XCM比较角化龈宽度增量更明显;根面覆盖率指标差异无统计学意义;PRF、XCM可以明显降低患者术后的疼痛感。结论:研究表明,自体软组织替代材料与CTG相比,对于治疗牙龈退缩的效果并没有显著差异,但是前者可以降低患者术后疼痛感。